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Introducción al mundo microbiano Víctor de Lorenzo Centro Nacional de Biotecnología CSIC, Madrid (Spain) Nuestro sistema de interés NO2 CH3 Cl Cl Cl Cl NH2 CH3 (CH)n CH3 CH3 NO2 Cl CH3 OH Cl CH3 OH BIO-REMEDIACION A Tóxico BIO-REMEDIACION A Tóxico B Inocuo BIO-REMEDIACION A Tóxico B Inocuo Catalizador biológico BIO-REMEDIACION A Tóxico B Inocuo Catalizador biológico BIO-REMEDIACION CO2 + H2O mineralización B C transformación B inmovilización Donde buscar esos Biocatalizadores? Approximate timing of major events in the history of life on Earth Los tres dominios del árbol de la vida LUCA Propiedades microbianas de interés medioambiental • Rutas biodegradativas para compuestos recalcitrantes • Mineralización de especies químicas toxida • eliminación del azufre orgánico del c. fósiles • biosensoers • Química verde • Tolerancia a metales pesados • volatilización • ensayos de biodisponibilidad/toxicidad • bioacumulacion:eliminación de iones tóxicos • biotransformaciones y re-speciacion • Producción de surfactantes: bioremediacion de petróleo Internal structure of bacterial and archaeal cells (prokaryotes) as compared with plant and animal cells (eukaryotes) Internal structure of bacterial and archaeal cells (prokaryotes) as compared with plant and animal cells (eukaryotes) Shapes and characteristics of free-living microorganisms Shapes and characteristics of free-living microorganisms. A ciliated protozoan Features of viruses Fossilized stromatolite Cultivos de enriquecimiento Growth curve of bacteria in laboratory medium Streaking plates to obtain isolated colonies Serial dilution of cultures to obtain isolated colonies Plaque assay for determining number of infectious viral particles in a sample Bacteria undergoing cell division Gram stain procedure Structure of gram-positive cell well Structure of gram-negative cell wall Structure of flagella Bacterial motion mediated by flagella Chemotaxis Circular representation of a genome sequence Overview of transcription and translation and antibiotics that inhibit these processes at different levels The disk diffusion antimicrobial susceptibility test Antimicrobial susceptibility assays that give MIC values Antimicrobial susceptibility assays that give MIC values Mutations occurring at different sites in the DNA Célula procariótica (bacterias) Nutrientes Contaminantes Membrana Ribosomas ADN
