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Honors Biology CHAPTER 8 REVIEW “Sex in the Cell City” #1 Contrast asexual and sexual reproduction: • ASEXUAL • 1 parent • Forms identical cells • Forms 2 daughter cells • SEXUAL • 2 parents • Forms different cells • Forms 4 daughter cells #2 Compare: Chromatin, Chromosomes, Chromatids ANSWER • ALL ARE DNA + PROTEIN • Chromatin – loose in interphase • Chromosomes – dense and coiled in mitosis- connected by a centromere Chromatid – condensed - paired with identical sister chromatid To determine the number of chromosomes, count the centromere regions • There are 3 chromosomes here There are 6 chromosomes here #3 What do you call… • A fertilized egg • Zygote • Fertilization • Joining of the egg and sperm #4 • What do you call asexual reproduction in prokaryotes? • Binary fission • What do you call asexual reproduction in eukaryotes? • mitosis Asexual and Sexual Repro • How do the chromosome numbers compare before and after reproduction in each? • EXAMPLE: If the original cell had 20 chromosomes, how many chromosomes would each cell have after each type of reproduction? ANSWER • Sexual Reproduction -resulting cells have half the number of chromosomes • (start with 20 – end with 10 chromosomes) • Diploid to haploid • Asexual Reproduction -resulting cells have the identical number of chromosomes • (start and end with 20 chromosomes) • Diploid to diploid #5Contrast DNA in prokaryotes and eukaryotes • Shape • Size • complexity ANSWER #5 Chromosomes differ in… • PROKARYOTES • EUKARYOTES • One Circular • Many Long chromosome strands of chromosomes • Smaller (3,000 genes in • Larger ( 100,000 bacterium) genes in humans) • simpler • More complex #4 Compare the number of genes: • Humans to bacteria • ANSWER: • 100,000 to 3,000 6. Put in correct order • Telophase • Anaphase • Metaphase • Prophase • Prometaphase 6. ANSWER • P PM M A T –Prophase –Prometaphase –Metaphase –Anaphase –Telophase 7. How does nuclear membrane change? •In prometaphase •dissolves •In telophase •reforms 8. What is…? •Cytokinesis •Division of the cytoplasm 8. How is cytokinesis different in… • PLANT CELLS • ANIMAL CELLS 8. ANSWER • PLANT CELLS • Form cell plate • ANIMAL CELLS • Form a cleavage furrow by pinching in the cytoplasm 8. (continued) Define: • Cell plate • Cleavage furrow • Cleavage Furrow • Pinching of the cytoplasm by actin • Cell plate pulling like a • In plant cells the drawstring cell wall will form 8. ANSWER 9. What goes on in each? •G1 •S •G2 ANSWER • G1grows in size, increases organelles • S DNA synthesis (replicates) • G2 makes centrioles for cell division + growth What is the purpose of… • Mitosis? A. make bigger cells • B. make exact copies of cells in two daughter cells • C. make copies of cells with twice the number of chromosomes • D. decrease the number of cells ANSWER •B. make exact copies of cells in two daughter cells 10. Locate • Chromatids • Centromeres • Asters • Centrioles • spindles 10. ANSWER Chromatids Centromeres asters Spindles centrioles 11. Spindles • What is the difference between the kinetochore and the non-kinetochore spindles? • Kinetochore spindles are attached to the chromatids and shorten • Non-kinetochore spindles go pole-to-pole and lengthen the cell 11. What makes the… • Chromatids move to the poles (centrioles)? • Kinetochore spindles shorten and motor proteins power the movement 11. ANSWER • kinesin motor protein • Motor Proteins (dynein)-use ATP to grab and move the chromatid 12. Growth Factor • Chemical produced outside of the cell to start the cells to begin the cell cycle • EX: when you have a cut • 12. What is the purpose of a growth factor? • naturally occurring substance capable of stimulating cellular growth • Remember the mice would healing article at the beginning of the year? What did VEGF do? (grape-seed article) • Create blood vessels if at an injury site 13. MATCHING 1 Cells divide until reach a definite limit (other cells, walls) 2 cells need contact to grow 3 cells continue to grow Uncontrollably A. anchorage dependence B. cancer cells C. densitydependent inhibition 13. MATCHING ANSWERS 1-C Cells divide until reach a definite limit (other cells, walls) 2-A cells need contact to grow 3-B cells continue to grow Uncontrollably A. anchorage dependence B. cancer cells C. densitydependent inhibition 14. MATCH THE CANCERS • • • • • A. carcinoma B. sarcoma C. lymphoma D. leukemia E. metastasis • 1. spread of cancer cells beyond site • 2. cancer from bone marrow • 3. cancer from outer skin or linings • 4. support tissue cancers (bone, muscle) • 5. cancer of lymphatic system 14. MATCH THE CANCERS • • • • • 3 A. carcinoma 4 B. sarcoma 5 C. lymphoma 2 D. leukemia 1 E. metastasis • 1. spread of cancer cells beyond site • 2. cancer from bone marrow (makes WBC) • 3. cancer from outer skin or linings • 4. support tissue cancers (bone, muscle) • 5. cancer of lymphatic system 14. MATCH THE CANCERS A. Benign B. Malignant C. tumor • 1. tumor growth spread to other tissue • 2. abnormal growth but not beyond original site • 3. abnormal growth of cells 14. MATCH THE CANCERS A. 2 Benign B. 1 Malignant C. 3 tumor • 1. tumor growth spread to other tissue • 2. abnormal growth but not beyond original site • 3. abnormal growth of cells 15. What is the name of the gene that... • Gene that causes breast cancer • P27 (on chromosome 12 FYI) • Spellchecks DNA for errors and repairs them? • p53 16. • MITOSIS a. 2n to 2n (diploid to diploid) b. forms cells identical to parent cell • MEIOSIS • a. 2n to n • (diploid to haploid) • b. forms cells different from parent cell 16. (cont.) • MITOSIS • c. 1 divison • d. forms 2 daughter cells • e. makes somatic cells • MEIOSIS • c. 2 divisions • d. forms 4 daughter cells • e. makes sex cells 17. What is the name of… • The protein that signals each phase of the cell cycle to start? • Cyclins • (build up and break down) How do cyclins and cdk’s work together? • Cdk is an enzyme that attaches to cyclin to form MPF (maturation promotion factor) to begin that part of the cell Cycle Phosphate group activates with energy 18. How are cancer cells... •Abnormal in the cell cycle? ANSWER • Do not have a properly functioning cell cycle control systemgrow uncontrollably 18. What body cells... • Divide a lot? • Not at all after formed? 18. What cells… • Divide a lot • skin • blood • digestive tract • Don’t divide after formed • Nerve (brain) • muscle 18. What part of the cell cycle… • Is when cells that do not divide leave the cell cycle or go to differentiate? • G0 19. Name the Phase of Mitosis • Chromatin thickens • Prophase • prometaphase • nuclear envelope disappears • nuclear envelope reforms • telophase 19. Name the Phase of Mitosis • Centrioles moving to the opposite poles • spindle fibers form • cell plate forms • Prophase • prophase • Telophase/ cytokinesis 19. Name the Phase of Mitosis • Chromosomes line up at the equator • Metaphase • cytoplasm divides • cytokinesis Identify the Phase of Mitosis • anaphase Identify the Phase of Mitosis • prophase Identify the Phase of Mitosis • telophase Identify the Phase of cell cycle • Interphase Identify the Phase of Mitosis • prometaphase Identify the Phase of Mitosis • Metaphase 20. Somatic or Sex Cell? • • • • • • • • Skin Somatic (body cell) Sperm Sex Blood Somatic Egg sex 21. Difference between… • Sex chromosomes and autosomes? • ANSWER: Sex chromosomes are X and Y (usually #23) Autosomes are rest of chromosomes, in human karyotype #1-22 22. What is the difference… • Between diploid and haploid (and symbols)? • ANSWER:Diploid is 2n with 2 sets of chromosomes in a cell • Haploid is n with one set of chromosmes in a cell • What is the diploid and the haploid number for humans? • ANSWER: diploid – 2n = 46 • haploid – n = 23 If given the diploid number, what is the haploid number? • Diploid • 40 • Haploid • 20 • 24 • 12 • 16 •8 23. Which is not a male? • XXY • XY • XO • XXXY • Which is the normal male? • XO is not a male and XY is the normal male 24. How are they related? • Tetrad and synapsis • Tetrad are 4 homologous chromosomes and synapsis is the joining of these 4 to form a tetrad 25. Crossover • When does it occur? • During prophase I • Between which two chromosomes does it occur? • Two homologous chromosomes • What is the site where it occurs? • chiasma 25. What is a chiasma? A B C 25. CHIASMA ANSWER Point where crossing over occurs between two inner chromosomes 26. How many possible combinations? • What is the formula? • 2n • What does n stand for? • Haploid number • How many combos if n = 2? •4 26. (cont.) How many possible combinations? • How many combos if n = 3? • 8 = (2 x 2 x 2) • How many combos if n = 23? • About 8 million Which does not belong? • Skin cell • Diploid • Gamete • Somatic cell • ANSWER: gamete Which does not belong? • • • • • • 2N Haploid Gamete Sperm Egg ANSWER: 2N 27. Who determines… • The sex of the offspring? Father or mother • • • • • FATHER WHY? If he donates an X = child is girl (XX) If he donates a Y = child is a boy (XY) Mother only can donate an X 28. What are homologous chromosomes? What are sister chromatids? • ANSWER: Sister chromatids are exact duplicates (from S phase) • Homologous chromosome are two sister chromatids from the Father and two sister chromatids from the Mother (Example : 4 of the #1 chromosomes) #29 What are gametes? • • • • • A. autosomes B. female chromosomes C. sex cells D. daughter cells ANSWER: Gametes are sex cells. For Human they are sperm (male) and egg (female) 29. What is the difference between • A gamete and a zygote? • A gamete is a sex cell (like egg and sperm) and a zygote if a fertilized egg (the joining of egg and sperm) 30. What phase of meiosis? 30. What phase of meiosis? A1 A2 M1 M2 P2 4 daughter cells P1 30. What phase of meiosis? 30. What phase of meiosis? • • • • • • • • • • • • A-I B. P1 early C.P1 late D. M1 E. F. A1 G. T1 H. Interkinesis I. P2 J. M2 K. A2 L. T2 M.4 daughter cells #31 Meiosis or Mitosis? • • • • • • Makes cells that are haploid? Meiosis Makes cells that are diploid? Mitosis Makes sex cells meiosis 31. Mitosis or Meiosis? • Makes identical cells? • Mitosis • Makes similar, but not identical cells? • Meiosis 31. Mitosis or Meiosis? •Has 1 division •Mitosis •Has 2 divisions •Meiosis 31. Mitosis or Meiosis? • Which makes sex cells? • Meiosis • Which makes somatic cells? • Mitosis 31. How many cells form? • At the end of meiosis? • 4 haploid • At the end of mitosis? • 2 diploid 32. What 3 factors increase genetic variability after meiosis? • 1. independent orientation of chromosomes at metaphase I (which chromosome lined up on each side) • 2. random fertilization (which sperm joined which egg) • 3. crossing over • (ALSO MUTATION…coming in next unit) In what phase… • Does synapsis (joining of tetrads) form? • Prophase I • Prophase II • Metaphase I • Metaphase II • ANSWER: Prophase 1 Hair color • Is a gene on a chromosome. The variations of it, such as brown, red, or black would be known as _________. • Alleles are the choices of a gene What is this showing? • Crossing over • It occurs during what phase? • Prophase I If the testicle cell has • 50 chromosomes, how many chromosomes does the sperm have? 100 50 25 10 • ANSWER: 25 Give the missing info • If the Diploid Number is… • 46 • 18 • 72 • Then the Hapoid Number is… • 23 • 9 • 36 Which phase of meiosis? • Where are the chromatids? • Meiosis I or II? • Anaphase I Which phase of meiosis? • Where are the chromosomes going? How many cells? • Anaphase II