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Navigating Expression Data in
FlyBase
2011 Drosophila Research
Conference
How To Find Expression Data
When & where is my favorite gene expressed?
Report Pages
GBrowse
BROWSE
What genes are expressed at a given time & tissue?
SEARCH
TermLink
QuickSearch
QueryBuilder
Similarity-Based Comparison
Two Kinds of Expression Data In FlyBase
Old School:
paper by paper, gene by gene
painstaking & qualitative
High Throughput:
data set by data set
expansive & quantitative
Browsing Curated Expression Data
Browsing of Curated Expression Data
Browsing of Curated Expression Data
Location of External Expression Data
Location of External Expression Data
Reporter Expression Data
Reporter Expression Data
Curated Expression Data
Reporter Expression Data
Browse High-Throughput Expression Data:
Gene Reports
Browse High-Throughput Expression Data:
Gene Reports
Browse High-Throughput Expression Data:
Gene Reports
Browse High-Throughput Expression Data:
Gene Reports
Browse High-Throughput Expression Data:
Gene Reports
GBrowse: MicroArray Expression Data
GBrowse: RNA-Seq Coverage Data
GBrowse: RNA-Seq Coverage Data
GBrowse: Other High-Throughput Data Tracks
GBrowse: Exon Junctions
Searching Expression Data
Literature-curated
TermLink
High-throughput
Similaritybased
QuickSearch
Granularity
QueryBuilder
TermLink: A single parameter
Searching Curated Data: Term Link
Searching Curated Data: Term Link
Searching Curated Data: Term Link
CV Term Reports: Identifying related records
Searching Curated Data: Term Link
Searching Curated Data: Quick Search
Searching Curated Data: Quick Search
Searching Curated Data: Quick Search
Searching Curated Data: Query Builder
Searching Curated Data: Query Builder
Searching Curated Data: Query Builder
Searching Curated Data: Query Builder
Searching Curated Data: Query Builder
Searching High-Throughput Data: QuickSearch
Searching High-Throughput Data: QuickSearch
Searching High-Throughput Data: QuickSearch
Searching High-Throughput Data: HitList
Refinement
Searching High-Throughput Data: HitList
Refinement
Searching High-Throughput Data: HitList
Refinement
Searching High-Throughput Data: HitList
Refinement
Similarity-based searches
Similarity-based searches
• Based upon temporal expression pattern
• Correlation of increases and decreases in
expression levels
• Absolute expression level not important
• GO terms matching those for the input
gene are highlighted.
Summary
• There are different ways to search expression data depending upon:
• the type of data
• the type of search you want to do
• Literature curated data and high throughput data must be searched
separately.
• Literature curated data and high throughput data can both be
browsed on the gene report.
• Use the gene expression report to view ALL the curated data for a
particular gene.
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