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Navigating Expression Data in FlyBase 2011 Drosophila Research Conference How To Find Expression Data When & where is my favorite gene expressed? Report Pages GBrowse BROWSE What genes are expressed at a given time & tissue? SEARCH TermLink QuickSearch QueryBuilder Similarity-Based Comparison Two Kinds of Expression Data In FlyBase Old School: paper by paper, gene by gene painstaking & qualitative High Throughput: data set by data set expansive & quantitative Browsing Curated Expression Data Browsing of Curated Expression Data Browsing of Curated Expression Data Location of External Expression Data Location of External Expression Data Reporter Expression Data Reporter Expression Data Curated Expression Data Reporter Expression Data Browse High-Throughput Expression Data: Gene Reports Browse High-Throughput Expression Data: Gene Reports Browse High-Throughput Expression Data: Gene Reports Browse High-Throughput Expression Data: Gene Reports Browse High-Throughput Expression Data: Gene Reports GBrowse: MicroArray Expression Data GBrowse: RNA-Seq Coverage Data GBrowse: RNA-Seq Coverage Data GBrowse: Other High-Throughput Data Tracks GBrowse: Exon Junctions Searching Expression Data Literature-curated TermLink High-throughput Similaritybased QuickSearch Granularity QueryBuilder TermLink: A single parameter Searching Curated Data: Term Link Searching Curated Data: Term Link Searching Curated Data: Term Link CV Term Reports: Identifying related records Searching Curated Data: Term Link Searching Curated Data: Quick Search Searching Curated Data: Quick Search Searching Curated Data: Quick Search Searching Curated Data: Query Builder Searching Curated Data: Query Builder Searching Curated Data: Query Builder Searching Curated Data: Query Builder Searching Curated Data: Query Builder Searching High-Throughput Data: QuickSearch Searching High-Throughput Data: QuickSearch Searching High-Throughput Data: QuickSearch Searching High-Throughput Data: HitList Refinement Searching High-Throughput Data: HitList Refinement Searching High-Throughput Data: HitList Refinement Searching High-Throughput Data: HitList Refinement Similarity-based searches Similarity-based searches • Based upon temporal expression pattern • Correlation of increases and decreases in expression levels • Absolute expression level not important • GO terms matching those for the input gene are highlighted. Summary • There are different ways to search expression data depending upon: • the type of data • the type of search you want to do • Literature curated data and high throughput data must be searched separately. • Literature curated data and high throughput data can both be browsed on the gene report. • Use the gene expression report to view ALL the curated data for a particular gene.