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Gregor Mendel Mendel’s Conclusions • In his crosses each trait had a Dominant Factor which masked, or dominated the other factor for a specific trait. • the other trait we call the Recessive Factor. • In a true-breeding cross (BBxbb) the recessive factor is masked in F1 but appears in F2 Mendel’s Conclusions • Law of Segregation: Each organism contains two factors for each trait; factors segregate in the formation of gametes. When two gametes combine during fertilization, the offspring have two factors controlling a specific trait. • Law of Independent Assortment: states that factors for different characteristics are distributed to gametes independently. Genetics Vocabulary • Gene - the unit of heredity; a section of DNA sequence encoding a single protein • Alleles - two genes that occupy the same position on homologous chromosomes and cover the same trait • Locus - a fixed location on a strand of DNA where a gene or one of its alleles is located Genetics Vocabulary • Homozygous - having identical genes (one from each parent) for a particular characteristic. • Heterozygous - having two different genes for a particular characteristic. Genetics Vocabulary • Phenotype - appearance • Genotype - genetic makeup Genetics Vocabulary • Dominant - the trait that appears in the heterozygous condition. • Recessive - the trait that is masked in the heterozygous condition. Genetics Vocabulary • Monohybrid cross - cross involving a single pair of genes, one trait • P – “parents” • F1 – “kids” • F2 – “grandkids” Genetics Vocabulary • Punnett squares - used to aid in predicting the probability that certain traits will be inherited by offspring • Probability - is the likelihood that a specific event will occur or is the likely outcome a given event will occur from random chance How To: Monohybrid Crosses On the Back of Your Notes Steps Notes Examples Monohybrid Crosses (only one trait) • Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a white male rabbit and a heterozygous black female rabbit. Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a White male rabbit and a heterozygous black female rabbit. Black is dominant to white in bunnies. Step Notes 1. Determine the Will be stated in dominant and the problem Recessive trait Example Black is dominant to white in bunnies Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a White male rabbit and a heterozygous black female rabbit. Black is dominant to white in bunnies. Step Notes Example 2. Assign letters for the trait Dominant trait gets the capital letter B = black b = white Recessive traits gets the lower case letter Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a White male rabbit and a heterozygous black female rabbit. Black is dominant to white in bunnies. Step Notes Example 3. Determine Each parent has White male = bb genotype for two alleles parents Heterozygous black female = Bb Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a White male rabbit and a heterozygous black female rabbit. Black is dominant to white in bunnies. Step Notes 4. Put parents on Separate the square parental alleles Example Bb x bb B b b b Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a White male rabbit and a heterozygous black female rabbit. Black is dominant to white in bunnies. Step Notes Example 5. Determine genotype of offspring Punnett square holds offspring genotypes Bb x bb Genotype = letter code B b b Bb bb b Bb bb Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a White male rabbit and a heterozygous black female rabbit. Black is dominant to white in bunnies. Step Notes Example 6. Determine genotype ratio Count offspring in the Punnett square genotype ratio = 2 Bb: 2 bb There are two correct ways to represent your data 2/4 Bb, 2/4 bb Ineeda Know Ph.D. is trying to identify the potential genotypes of offspring of a White male rabbit and a heterozygous black female rabbit. Black is dominant to white in bunnies. Step Notes Example 7. Determine phenotype ratio Phenotype = what they look like *ratio MUST add up to the number of boxes* Count offspring in the Punnett square phenotype ratio 2 black:2 white Practice Problems A pure-breed white flower crosses with a pure-breed purple flower. Purple is dominant to white. • • • • Determine the genotypes of the flowers. Show the punnett square What is the genotype of the F1 generation What is the phenotype of the F1 generation Genotype = Pp Phenotype = Purple More Practice A homozygous black guinea pig is crossed with a heterozygous black guinea pig . B = black b = brown • Determine the genotypes of the parental guinea pigs. • Show the punnett square. • What are the genotypes? • What are the genotype ratio? • What are the phenotypes? • What are the phenotype ratios? Genotypes = BB and Bb Genotype Ratio = 2BB:2Bb Phenotypes = All Black Even More Practice If you had a black guinea pig how could you tell it’s genotype was homozygous and heterozygous? Use a test cross: cross with a pure breed recessive and look at the F1 generation