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T t T Tt t MIDDLE SCHOOL GENETICS SINGLE TRAIT PUNNETT SQUARE ¼ OBJECTIVE 2 • THE STUDENT WILL DEMONSTRATE AN UNDERSTANDING OF LIVING SYSTEMS AND THE ENVIRONMENT STUDENT EXPECTATION • 8.11 STUDENTS WILL MAKE PREDICTIONS ABOUT POSSIBLE OUTCOMES OF VARIOUS GENETIC COMBINATIONS OF INHERITED CHARACTERISTICS T T T TT t Tt HIGHLIGHT OF OBJECTIVE 2 • SINGLE-TRAIT PUNNETT SQUARES MAY BE USED, AND STUDENTS WILL BE EXPECTED TO PREDICT GENETIC OUTCOMES. ¾ 75% 3:1 TOOLS TO KNOW A PUNNET SQUARE IS A TOOL USED TO PREDICT THE POSSIBLE GENOTYPES FOR THE OFFSPRING OF TWO KNOWN PARENTS. PARENT’S GENES PARENT’S GENES TERMS TO KNOW ALLELES DIFFERENT FORMS OF A TRAIT THAT A GENE MAY HAVE HOMOZYGOUS AN ORGANISM WITH TWO ALLELES THAT ARE THE SAME HETEROZYGOUS AN ORGANISM WITH TWO DIFFERENT ALLELES FOR A TRAIT T,t TT, tt Tt, Gg TERMS TO KNOW HYBRID SAME AS HETEROZYGOUS DOMINANT A TRAIT THAT DOMINATES OR COVERS UP THE OTHER FORM OF THE TRAIT Tt, Gg REPRESENTED BY AN UPPERCASE LETTER T G OR RECESSIVE THE TRAIT BEING DOMINATED OR COVERED UP BY THE DOMINATE TRAIT REPRESENTED BY A LOWER CASE LETTER t g or TERMS TO KNOW PHENOTYPE THE PHYSICAL APPEARANCE OF AN ORGANISM (WHAT IT LOOKS LIKE) TALL, SHORT, GREEN, WRINKLED GENOTYPE THE GENE ORDER OF AN ORGANISM (WHAT ITS GENES LOOK LIKE) TT, GG, Tt, gg Gg, tt RATIO THE RELATIONSHIP IN NUMBERS BETWEEN TWO OR MORE THINGS 3:1, 2:2, 1:2:1 HOW TO USE A MONOHYBRID (ONE TRAIT) PUNNETT SQUARE THE PARENTS’ ALLELES GO ON THE OUTSIDE OF THE SQUARE BB X bb b b B B HOW TO USE A MONOHYBRID (ONE TRAIT) PUNNETT SQUARE THE PARENTS’ ALLELES GO ON THE OUTSIDE OF THE SQUARE b THE ORDER DOES NOT MATTER IN THE BOXES, BUT UPPERCASE FIRST IS A GOOD RULE b B B b B b B B b B b DROP THE LETTERS ON THE TOP, INTO EACH SQUARE MOVE EACH LETTER ON THE SIDE, INTO EACH SQUARE HOW TO USE A MONOHYBRID (ONE TRAIT) PUNNETT SQUARE WHAT DO THE RESULTS SHOW? RESULTS: B B PHENOTYPIC: IF B IS THE DOMINANT ALLELE FOR BLACK AND b IS THE RECESSIVE ALLELE FOR BROWN 100% BLACK b Bb GENOTYPIC: 100% Bb b Bb THEN WE CAN MAKE PREDICTIONS ABOUT THE OUTCOMES Bb 4:0 RATIO, BLACK TO BROWN Bb 4:0 ALL Bb HOW TO USE A PUNNETT SQUARE LET’S LOOK AT ANOTHER PUNNETT SQUARE AND PREDICT THE OUTCOME WHAT ARE THE RESULTS? T t PHENOTYPIC: T T T Tt T IS THE DOMINANT ALLELE FOR TALLNESS t IS THE RECESSIVE ALLELE FOR SHORTNESS 75% TALL 25% SHORT 3 TO 1 RATIO: TALL TO SHORT GENOTYPIC: t T t t t 1TT: 2Tt: 1tt 1:2:1 RATIO 25 %TT, 50% Tt, 25% tt PRACTICAL APPLICATION OF PUNNETT SQUARES THE ALLELES OF A PARTICULAR SPECIES OF DOG CAN BE EITHER D (NORMAL HEIGHTH) OR d (DWARF). THE HETEROZYGOUS (Dd) AND HOMOZYGOUS DOMINANT (DD) FORM OF THIS DOG LOOK THE SAME (TALL). IF YOU FOUND A STRAY DOG OF THIS BREED, HOW COULD YOU DETERMINE ITS GENOTYPE? PRACTICAL APPLICATION OF PUNNETT SQUARES COULD A DOG BE CROSSED WITH ANOTHER DOG TO DETERMINE IF HE WAS PUREBRED FOR TALLNESS? WHAT GENOTYPE SHOULD THE DOG HAVE THAT IS BEING USED FOR THE CROSS? PRACTICAL APPLICATION OF PUNNETT SQUARES IF THE DOG IS PUREBRED (DD), IT DOESN’T MATTER WHAT YOU CROSS IT WITH, THE OFFSPRING WILL ALWAYS LOOK LIKE THE DOMINANT. D D DD d Dd Dd D D DD DD D DD DD D D d Dd Dd d Dd D DD D Dd PRACTICAL APPLICATION OF PUNNETT SQUARES WHAT WOULD BE THE MOST EFFECTIVE CROSS FOR DETERMINING IF THE DOG IS HETEROZYGOUS (Dd) ? CROSSING IT WITH A PUREBRED (DD) WILL NOT HELP. D WHAT WOULD THE RESULTS BE IF YOU CROSSED IT WITH ANOTHER HETEROZYGOUS? WHAT WOULD THE RESULTS BE IF YOU CROSSED IT WITH A HOMOZYGOUS RECESSIVE (dd)? d D DD Dd d Dd dd d d D d Dd dd Dd dd PRACTICAL APPLICATION OF PUNNETT SQUARES D d D DD Dd d d Dd dd d THE HETEROZYGOUS CROSS WOULD ONLY GIVE YOU A 25% CHANCE OF THE RECESSIVE TRAIT APPEARING. D d Dd dd Dd dd THE MOST EFFECTIVE CROSS WAS USING THE HOMOZYGOUS RECESSIVE. THIS WOULD GIVE A 50% CHANCE OF THE RECESSIVE TRAIT APPEARING. THIS PROCESS IS CALLED A TEST CROSS. IN A LITTER OF DOGS, IF A RECESSIVE DOG APPEARS, THEN YOU KNOW THAT THE ORIGINAL DOG WAS NOT A PUREBRED. TAKS FORMATTED ITEMS IN DROSOPHILA MELANOGASTER (FRUIT FLIES), RED EYE COLOR (R) IS DOMINANT OVER BROWN EYE COLOR (r). IF THE FLIES IN THE PICTURE WERE CROSSED, WHAT PERCENT OF THEIR OFFSPRING WOULD BE EXPECTED TO HAVE BROWN EYES? ANSWER: 50% TAKS FORMATTED ITEMS H 1. WHICH OF THE FOLLOWING HAS THE hh GENOTYPE? A. B. C. D. 1&3 2 4 NONE H 4 1 h 3 2 2. WHICH OF THE FOLLOWING IS A TRUE STATEMENT? A. B. C. D. h INDIVIDUAL 4 IS RECESSIVE INDIVIDUALS 1 & 3 ARE HETEROZYGOUS INDIVIDUAL 2 IS DOMINANT ALL INDIVIDUALS ARE FEMALE TAKS FORMATTED ITEMS 3. IF B IS THE ALLELE FOR BLACK FUR AND b IS THE ALLELE FOR WHITE FUR, WHAT PERCENT WOULD BE BLACK? A. B. C. D. 25% 50% 100% 75% B b B BB Bb b Bb bb 4. WHAT FRACTION IS HOMOZYGOUS DOMINANT IN THE ABOVE CROSS? A. B. C. D. 1/2 1/4 1/3 3/4 TAKS FORMATTED ITEMS B 5. IN THIS CROSS, WHAT IS THE RATIO OF BB TO Bb? A. B. C. D. 3:1 4:1 2:2 0:4 B B BB BB b Bb Bb BIBLIOGRAPHY • Mendel picture provided by wikipedia.com •Dog and tree pictures provided by Microsoft ClipArt •Graphics from TEA/ TAKS information booklet Middle School Science Grade 8 •Dice graphic from Microsoft Clipart