Download Evolution of Genes and Chromosomes

Evolving Resistance:
Research Goals and
Goods
Georgiana May
Brett Couch
Ethy Cannon
University of Minnesota
Education
Training
Evolution
Genes

Map
Information
Genome
QuickTime™ and a
TIFF (LZW) decompressor
are needed to see this picture.
Resistance
Resources
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Ever since Grube et al.
Chromosome 11S
I2
R3,7
QuickTime™ and a
TIFF (LZW) decompressor
are needed to see this picture.
RB
I2- homologous
sequences in Solanaceae
Tomato
Potato
S. dem.
Eggplant
Pepper
Tobacco
Couch et al. MPMI 2006
I2- h NBS sequences
under positive selection
Positive
Selection
NBS
LRR
NBS and LRR regions reassociate through time
Contributions and
resources
• Birth-death model applies to R
gene evolution
• 232 sequences from 6 species
– positive selection
– evolving function?
seek new resistance function in
fewer than 10 lineages
• Brett Couch
– David Schladt
I2 lineages maintained
12 million years
Ancient
duplications
Contributions and
resources
• Long term maintenance of R
gene lineages - 12 my
• 232 sequences from 6 species
– maintained through speciation
– maintain function?
seek non-host R in fewer than 10
lineages
• Brett Couch
I2-h map to
chromosomes 8,9,11
in tomato
8
B
11
9
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B
B
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B
IL Block
B BAC contig
250 tomato BAC
clones hybridized to
I2 probes
Oligo probes
hybridize to
specifc I2
homologs
Map I2-containing BAC clones
to IL segment
PCR length polymorphisms in parent lines
BAC end polymorphisms segregate in
the L. pinnellii mapping population
Contributions and
resources
• Verified Pan, Fluhr results
– chromosomes 8,9,11
– not on 3,4 (Pan)
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• New region - 9 I
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• Ancient chromosomal
duplications carry R gene
duplications
Information
Genes

Genome
• Brett Couch
– Deren Eaton

GENEOLOGY
 Information
GENOME
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R
Baumgarten et al. 2002
Goods
QuickTime™ and a
TIFF (LZW) decompressor
are needed to see this picture.
solevol
demo site
Ethalinda Cannon
examples
QuickTime™ and a
TIFF (LZW) decompressor
are needed to see this picture.
Resistance
Resources
• 75 distinct I2-h lineages
– over 6 species
– http://solevol.ccgb.umn.edu/
• Genome locations
• Development of
evolutionary models
• Training
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Education
Training
Evolution
Genes

Map
Information
Genome
QuickTime™ and a
TIFF (LZW) decompressor
are needed to see this picture.
Resistance
Resources
Q
u i ck Ti m e ™ an d a
TI n
F ee
F ( LZ
)o
d s
ec ee
om
ar e
de W
d t
t hpr
is es
p
Q ui
ck W
Ti) m
e ™
deass or
T I
Fne
F
( LZ
co an
m
a e
r
ed
ed t
ode
s ee
hpr
t
i s
pi c t
u r e.
so
r e
i ct ur
.
Q
u( LZ
i ck Ti) d
m ec
e ™
an des
a
TI n
F ee
F
om
ar e
de W
d t o s
ee
t hpr
is p
so
r e
i ct ur
.