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The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD). Furthermore, it has a proven activator domain (A) and repressor domain (R). Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote cell growth Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit cell growth. mRNAs with HD alone never occur. WT mRNAs with A-R-HD code for non-functional proteins. Arl Lab:_______________ 20pts E1 E2 1 A 2 R HD Express TxFactor E1 Express TxFactors E1, E2, E3 E1 and E2 are both needed to drive RBP typeA expression E1, E2, and E3 (all3) needed to drive RBP typeB expression E1, E2, and E3 (all3) needed to drive Arl expression E3 means enhancer binding site RBP means RNA binding protein Express TxFactors E1, E2 Q1(15pts). Quiz 8: Biol 203 Fall 2013 Name:_______________ RBP binding sites (where RBP would bind in the transcribed RNA) If RBP typeA suppresses 3’ acceptor site usage in the unspliced RNA at position 1, then mark cells below where R-HD mRNAs will be made. If RBP typeB suppresses 3’ acceptor site usage in the unspliced RNA at position 2, then mark cells below where A-HD mRNAs will be made. 30 cells in WT Antennal Tissue A null allele within a RBP gene will give the same phenotype within the 30 cells as a null allele for Arl. Which one?______ Q2(5pts). Using a flow chart and a few words: please describe how first strand cDNA is derived from an mRNA? What does the first 5’ nucleotide of this first strand cDNA correspond to in the mRNA?__________________ What does the last 3’ nucleotide of this first strand cDNA correspond to in the mRNA? __________________ What does the first 5’ nucleotide of this first strand cDNA correspond to in the gDNA? __________________ What does the last 3’ nucleotide of this first strand cDNA correspond to in the gDNA? __________________ The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD). Furthermore, it has a proven activator domain (A) and repressor domain (R). Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote cell growth Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit cell growth. mRNAs with HD alone never occur. WT mRNAs with A-R-HD code for non-functional proteins. Arl Lab:_______________ 20pts E1 E2 1 A 2 R HD Express TxFactor E1 Express TxFactors E1, E2, E3 E1 and E2 are both needed to drive RBP typeA expression E1, E2, and E3 (all3) needed to drive RBP typeB expression E1, E2, and E3 (all3) needed to drive Arl expression E3 means enhancer binding site RBP means RNA binding protein Express TxFactors E1, E2 Q1(15pts). Quiz 8: Biol 203 Fall 2013 Name:_______________ RBP binding sites (where RBP would bind in the transcribed RNA) If RBP typeB suppresses 3’ acceptor site usage in the unspliced RNA at position 2, then mark cells below where A-HD mRNAs will be made. If RBP typeA suppresses 3’ acceptor site usage in the unspliced RNA at position 1, then mark cells below where R-HD mRNAs will be made. 30 cells in WT Antennal Tissue A null allele within a RBP gene will give the same phenotype within the 30 cells as a null allele for Arl. Which one?______ Q2(5pts). Using a flow chart and a few words: please describe how first strand cDNA is derived from an mRNA? What does the first 5’ nucleotide of this first strand cDNA correspond to in the gDNA?__________________ What does the last 3’ nucleotide of this first strand cDNA correspond to in the gDNA? __________________ What does the first 5’ nucleotide of this first strand cDNA correspond to in the mRNA? __________________ What does the last 3’ nucleotide of this first strand cDNA correspond to in the mRNA? __________________
