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The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD).
Furthermore, it has a proven activator domain (A) and repressor domain (R).
Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote cell growth
Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit cell growth.
mRNAs with HD alone never occur. WT mRNAs with A-R-HD code for non-functional proteins.
Arl
Lab:_______________
20pts
E1
E2
1
A
2
R
HD
Express TxFactor E1
Express TxFactors E1, E2, E3
E1 and E2 are both needed to drive RBP typeA expression
E1, E2, and E3 (all3) needed to drive RBP typeB expression
E1, E2, and E3 (all3) needed to drive Arl expression
E3
means enhancer binding site
RBP means RNA binding protein
Express TxFactors E1, E2
Q1(15pts).
Quiz 8: Biol 203 Fall 2013
Name:_______________
RBP binding sites (where RBP would bind in the transcribed RNA)
If RBP typeA suppresses 3’ acceptor site
usage in the unspliced RNA at position 1,
then mark cells below where R-HD mRNAs
will be made.
If RBP typeB suppresses 3’ acceptor site
usage in the unspliced RNA at position 2,
then mark cells below where A-HD mRNAs
will be made.
30 cells in
WT Antennal
Tissue
A null allele within a RBP gene will give the same phenotype within the 30 cells as a null allele for Arl. Which one?______
Q2(5pts).
Using a flow chart and a few words: please describe how first strand cDNA is derived from an mRNA?
What does the first 5’ nucleotide of this first strand cDNA correspond to in the mRNA?__________________
What does the last 3’ nucleotide of this first strand cDNA correspond to in the mRNA? __________________
What does the first 5’ nucleotide of this first strand cDNA correspond to in the gDNA? __________________
What does the last 3’ nucleotide of this first strand cDNA correspond to in the gDNA? __________________
The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD).
Furthermore, it has a proven activator domain (A) and repressor domain (R).
Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote cell growth
Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit cell growth.
mRNAs with HD alone never occur. WT mRNAs with A-R-HD code for non-functional proteins.
Arl
Lab:_______________
20pts
E1
E2
1
A
2
R
HD
Express TxFactor E1
Express TxFactors E1, E2, E3
E1 and E2 are both needed to drive RBP typeA expression
E1, E2, and E3 (all3) needed to drive RBP typeB expression
E1, E2, and E3 (all3) needed to drive Arl expression
E3
means enhancer binding site
RBP means RNA binding protein
Express TxFactors E1, E2
Q1(15pts).
Quiz 8: Biol 203 Fall 2013
Name:_______________
RBP binding sites (where RBP would bind in the transcribed RNA)
If RBP typeB suppresses 3’ acceptor site
usage in the unspliced RNA at position 2,
then mark cells below where A-HD mRNAs
will be made.
If RBP typeA suppresses 3’ acceptor site
usage in the unspliced RNA at position 1,
then mark cells below where R-HD mRNAs
will be made.
30 cells in
WT Antennal
Tissue
A null allele within a RBP gene will give the same phenotype within the 30 cells as a null allele for Arl. Which one?______
Q2(5pts).
Using a flow chart and a few words: please describe how first strand cDNA is derived from an mRNA?
What does the first 5’ nucleotide of this first strand cDNA correspond to in the gDNA?__________________
What does the last 3’ nucleotide of this first strand cDNA correspond to in the gDNA? __________________
What does the first 5’ nucleotide of this first strand cDNA correspond to in the mRNA? __________________
What does the last 3’ nucleotide of this first strand cDNA correspond to in the mRNA? __________________