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Dr. SUKENDAH What is Biotechnology Biotechnology is the application of scientific and engneering principles to the processing of materials by biological agent to provide goods and services Aplikasi prinsip-prinsip ilmu dan rekayasa dalam pengolahan bahan-bahan menggunakan agen biologis untuk menghasilkan barang dan jasa What is Biotechnology What is Biotechnology manipulation of genes is called genetic engineering or recombinant DNA technology genetic engineering involves taking one or more genes from a location in one organism and either Transferring them to another organism Putting them back into the original organism in different combinations Related Knowledge & Industry Biologi Molekular Genetika Molekular Mikrobiologi Biokimia Biologi Sel Rekayasa Proses Bioteknologi Diagnosa Kesehatan Industri Farmasi Industri Fermentasi Industri Kimia Lingkungan dan Energi Industri Pangan dan Pakan Goals of Biotechnology To understand more about the processes of inheritance and gene expression To provide better understanding & treatment of various diseases, particularly genetic disorders To generate economic benefits, including improved plants and animals for agriculture and efficient production of valuable biological molecules Sejarah Perkembangan Jaman sebelum Louis Pasteur: Minuman beralkohol, makanan fermentasi Jaman Louis Pasteur: Alkohol, asam organik, pengolahan limbah Jaman antibiotika: Antibiotika, vaksin, transformasi obat Jaman pasca antibiotika: Asam amino, protein sel tunggal, imobilisasi Jaman bioteknologi modern: Antibodi monoklonal, rekayasa genetika Drought-tolerance in APX-expressing plants Water was withheld from transgenic tobacco plants that express ascorbate peroxidase (APX) and wildtype control plants (WT) for 18 days then the plants were re-watered for 2 days before the photograph was taken. Wildtype plants showed severe damaged while transgenic plants showed very little damage. Insect Resistance Testing of “Bt” cotton for resistance to bollworms Resistance to Fungal Disease Transgenic Wild-type Sunflower White mold resistance Control and transgenic tomato plants that express an oxalate oxidase gene construct two days after inoculation with the phytopathogenic fungus Sclerotinia which causes stem-rot diseases in a wide variety of economically important plants. Herbicide Resistance current: soybean, corn, canola, cotton, alfalfa coming: sugarbeet (on hold), lettuce, strawberry, wheat (on hold), Turf grass resistance gene from bacteria is source Modification of Cotton Fiber Length Transgenic Cotton that Express KC22 Biological production Food & Medicine production Industrial Fermentation