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Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
Drosophila speciation on the Hawaiian Islands.
Obbard D J et al. Mol Biol Evol 2012;29:3459-3473
© The Author 2012. Published by Oxford University Press on behalf of the Society for Molecular
Biology and Evolution.
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
Western Meadowlark
Eastern Meadowlark
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
4. Mechanical isolation - genitalia don't fit; limit pollinators
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
4. Mechanical isolation - genitalia don't fit
5. Gametic Isolation - gametes transfered but sperm can't fertilize egg; this
is a common isolation mechanism in species that spawn at the same time
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
4. Mechanical isolation - genitalia don't fit
5. Gametic Isolation - gametes transfered but sperm can't fertilize egg
B. Post-Zygotic Isolation
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
4. Mechanical isolation - genitalia don't fit
5. Gametic Isolation - gametes transfered but sperm can't fertilize egg
B. Post-Zygotic Isolation
1. Genomic Incompatibility - zygote dies
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
4. Mechanical isolation - genitalia don't fit
5. Gametic Isolation - gametes transfered but sperm can't fertilize egg
B. Post-Zygotic Isolation
1. Genomic Incompatibility - zygote dies
2. Hybrid Inviability - F1 has lower survival
Crazy hybrids
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
4. Mechanical isolation - genitalia don't fit
5. Gametic Isolation - gametes transfered but sperm can't fertilize egg
B. Post-Zygotic Isolation
1. Genomic Incompatibility - zygote dies
2. Hybrid Inviability - F1 has lower survival
3. Hybrid Sterility - F1 has reduced reproductive success
Horse: 64 chromosomes
Donkey: 62 chromosomes
Mule: 63 chromosomes
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
A. Pre-Zygotic Barriers
1. Geographic Isolation (large scale or habitat)
2. Temporal Isolation
3. Behavior Isolation - don't recognize one another as mates
4. Mechanical isolation - genitalia don't fit
5. Gametic Isolation - gametes transfered but sperm can't fertilize egg
B. Post-Zygotic Isolation
1. Genomic Incompatibility - zygote dies
2. Hybrid Inviability - F1 has lower survival
3. Hybrid Sterility - F1 has reduced reproductive success
4. F2 breakdown - F1's survive but F2's have incompatible combo's of genes
AABB
x
aabb
F1: AaBb = ok
F2:
A-B- = ok
A-bb = no
aaB- = no
aabb = ok
Species and Speciation
I. Species Concepts
II. Recognizing Species
III. Making Species - Reproductive Isolation
IV. Speciation
Speciation
Speciation is not a goal, or a selective product of adaptation. It is simply
a consequence of genetic changes that occurred for other reasons
(selection, drift, mutation, etc.).
Speciation
I. Modes:
Speciation
I. Modes:
A. Allopatric: Divergence in geographically separate populations
- Vicariance - range divided by new geographic feature
A
B
C
Almost all most recent
divergence events date to 3 my,
and separate species on either
side of the isthmus
Speciation
I. Modes:
A. Allopatric: Divergence in geographically separate populations
- Vicariance - range divided by new geographic feature
- Peripatric - divergence of a small migrant population
A
B
Abert’s Squirrel
Kaibab Squirrel
Crossed grand canyon to
the north during Ice Age
and isolated.
Speciation
I. Modes:
A. Allopatric: Divergence in geographically separate populations
- Vicariance - range divided by new geographic feature
- Peripatric - divergence of a small migrant population
B. Parapatric - neighboring populations diverge, even with gene flow
Speciation
I. Modes:
A. Allopatric: Divergence in geographically separate populations
- Vicariance - range divided by new geographic feature
- Peripatric - divergence of a small migrant population
B. Parapatric - neighboring populations diverge, even with gene flow
B. Parapatric - neighboring populations diverge, even with gene flow
Hybrid Backcross??
Hybrid
Speciation
I. Modes:
A. Allopatric: Divergence in geographically separate populations
- Vicariance - range divided by new geographic feature
- Peripatric - divergence of a small migrant population
B. Parapatric - neighboring populations diverge, even with gene flow
C. Sympatric: Divergence within a single population
C. Sympatric: Divergence within a single population
Maynard Smith (1966) - hypothesized this was possible if there
was disruptive selection within a population - perhaps as a specialist
herbivore/parasite colonized and adapted to a new host.
C. Sympatric: Divergence within a single population
Maynard Smith (1966) - hypothesized this was possible if there
was disruptive selection within a population - perhaps as a specialist
herbivore/parasite colonized and adapted to a new host.
Example: Hawthorn/Apple Maggot Fly (Rhagoletis pomonella)
Hawthorn maggot fly is a
native species that breeds on
Hawthorn (Crataegus sp.)
C. Sympatric: Divergence within a single population
Maynard Smith (1966) - hypothesized this was possible if there
was disruptive selection within a population - perhaps as a specialist
herbivore/parasite colonized and adapted to a new host.
Example: Hawthorn/Apple Maggot Fly (Rhagoletis pomonella)
Europeans brought apples to
North America. They are in
the same plant family
(Rosaceae) as Hawthorn.
C. Sympatric: Divergence within a single population
Maynard Smith (1966) - hypothesized this was possible if there
was disruptive selection within a population - perhaps as a specialist
herbivore/parasite colonized and adapted to a new host.
Example: Hawthorn/Apple Maggot Fly (Rhagoletis pomonella)
Europeans brought apples to
North America. They are in
the same plant family
(Rosaceae) as Hawthorn.
In 1864, apple growers
noticed infestation by Apple
Maggot flies...which were
actually just "hawthorn
flies"...
C. Sympatric: Divergence within a single population
Maynard Smith (1966) - hypothesized this was possible if there
was disruptive selection within a population - perhaps as a specialist
herbivore/parasite colonized and adapted to a new host.
Example: Hawthorn/Apple Maggot Fly (Rhagoletis pomonella)
races breed on
their own host
plant, and have
adapted to the
different seasons
of fruit ripening.
Only a 4-6%
hybridization rate.
Temporal, not
geographic,
isolation.
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977a and 1977b. Science.
Two species of green lacewings - generalist insect predators
Chrysopa downesi has one generation in early spring
C. carnea breeds and has three generations in summer
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977a and 1977b. Science.
Two species of green lacewings - generalist insect predators
Chrysopa downesi has one generation in early spring, then
diapause
C. carnea breeds has three generations in summer, no diapause
The differences are due to responses to photoperiod
C. downesi stops reproducing and goes into diapause under long
day length (summer), whereas C. carnea reproduces under long day
length.
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977a. Science 197:592.
The species are completely interfertile in the lab:
Did reciprocal matings: C. downesi x C. carea
Reared F1 offspring under long day length (16L:8D). Found all F1 did not
enter diapause (C. carnea photoperiod response is dominant).
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977a. Science 197:592.
Did F1 x F1 cross: Found 7% (~1/16) of F2 exhibited diapause at 16L:8D.
This is consistent with a model of 2 independently assorting autosomal
genes with complete dominance at each and an additive effect.
AABB
x
aabb
F1
all A-B- phenotype
F2
A-B- = 9/16
A-bb = 3/16
C. carnea photoperiod
aaB- = 3/16
aabb = 1/16.... ~ 7% C. downesi photoperiod
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977a. Science 197:592.
F1 x C. downesi backcross had 3:1 ratio, as expected of model.
AaBb x aabb
AaBb = .25
Aabb = .25
C. carnea photoperiod
aaBb = .25
aabb = .25
C. downesi photoperiod
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977b. Science 197:1298.
How did this temporal separation get established?
C. downesi is dark green and prefers hemlock forests
C. carnea is light green and prefers fields and meadows
Difference governed by a single locus where dark is incompletely
dominant.
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977b. Science 197:1298.
How did this temporal separation get established?
C. downesi is dark green and prefers hemlock forests
C. carnea is light green and prefers fields and meadows
Difference governed by a single locus where dark is incompletely
dominant.
Hypothesize that selection for different morphs in different habitats
created the stable dimorphism, reinforced by inbreeding within the
habitats.
intermediate
heterozygote
C. Sympatric: Divergence within a single population
But can a generalist speciate sympatrically?
Tauber and Tauber. 1977b. Science 197:1298.
How did this temporal separation get established?
C. downesi is dark green and prefers hemlock forests
C. carnea is light green and prefers fields and meadows
Difference governed by a single locus where dark is incompletely
dominant.
Hypothesize that selection for different morphs in different habitats
created the stable dimorphism, reinforced by inbreeding within the
habitats.
Selection then favored early breeding in C. downesi, as that is when
insects feeding on conifers are most abundant.
Speciation
I. Modes
II. Mechanisms
Speciation
I. Modes
II. Mechanisms
A. Progressive Genomic Incompatibility
Tilley et al. 1990. PNAS. Desmognathus ochrophaeus in western NC
1. correlation between geographic
distance and genetic distance
Tilley et al. 1990. PNAS. Desmognathus ochrophaeus in western NC
2. Placed sympatric and allopatric males
and females (reciprocal mating design)
together for an evening and examined the
cloaca of female in the morning for
presence of sperm packet.
Calculated "Coefficient of Isolation":
(sum of % of sympatric matings) - (sum of
% of allopatric matings)
2 = total isolation by sexual selection
0 = no differentiation by sexual selection
Speciation
I. Modes
II. Mechanisms
A. Progressive Genomic Incompatibility
B. Hybrid Incompatibility
- Dobzhansky and Müller (1930's)
Pairs of genes that work together diverge in
different populations
Speciation
I. Modes
II. Mechanisms
A. Progressive Genomic Incompatibility
B. Hybrid Incompatibility
- Dobzhansky and Müller (1930's)
Pairs of genes that work together diverge in
different populations
A1A1B2B2 works
A1A1B1B1 lethal
A1
A2A2B2B2 works
B1
A2A2B1B1 works
B. Hybrid Incompatibility
D. melanogaster and D. simulans
B. Hybrid Incompatibility
D. melanogaster and D. simulans
Cross female D. mel. x male D. sim - no sons
B. Hybrid Incompatibility
D. melanogaster and D. simulans
Cross female D. mel. x male D. sim - no sons
- Watanabe - 1970 - isolated a mutant strain of D.
simulans (w) that could make males with D.
melanogaster....
B. Hybrid Incompatibility
D. melanogaster and D. simulans
Cross female D. mel. x male D. sim - no sons
- Watanabe - 1970 - isolated a mutant strain of D.
simulans (w) that could make males with D.
melanogaster....
- Hypothesized that this strain had a mutant gene
partner that reestablished function with the D.
melanogaster partner gene... called it "lethal hybrid
rescue" (lhr).
B. Hybrid Incompatibility
D. melanogaster and D. simulans
Cross female D. mel. x male D. sim - no sons
- Watanabe - 1970 - isolated a mutant strain of D.
simulans (w) that could make males with D.
melanogaster....
- Hypothesized that this strain had a mutant gene
partner that reestablished function with the D.
melanogaster partner gene... called it "lethal hybrid
rescue" (lhr).
- Ashburner - 1980 - isolated a mutant strain of D.
melanogaster (a) females that could breed with D.
simulans males and produce sons ...called it
"hybrid male rescue" - hmr - X-linked
B. Hybrid Incompatibility
D. melanogaster and D. simulans
SYSTEM: (s-lhr dominant)
Ancestor: lhr, mhr
Male D. simulans: s-lhr, mhr
Female D. melanogaster: lhr, m-mhr(X)
s-lhr/lhr, m-mhr(X) = INVIABLE SONS
B. Hybrid Incompatibility
D. melanogaster and D. simulans
SYSTEM: (s-lhr dominant)
D. sim = s-lhr, hmr (X) x D. mel = lhr, m-hmr (X)
SONS GET : s-lhr/lhr, m-hmr/Y (only X) .... INVIABLE
B. Hybrid Incompatibility
D. melanogaster and D. simulans
SYSTEM:
D. sim = s-lhr, hmr (X) x D. mel = lhr, m-hmr (X)
SONS GET : s-lhr/lhr, m-hmr (only X) .... INVIABLE
(w)D. sim = lhr/s-lhr, hmr (X) x D. mel = lhr, m-hmr (X)
1/2 SONS GET lhr/lhr, m-hmr (ONLY X) = VIABLE
B. Hybrid Incompatibility
D. melanogaster and D. simulans
SYSTEM:
D. sim = s-lhr, hmr (X) x D. mel = lhr, m-hmr (X)
SONS GET : s-lhr/lhr, m-hmr (only X) .... INVIABLE
(w)D. sim = lhr/s-lhr, hmr (X) x D. mel = lhr, m-hmr (X)
1/2 SONS GET lhr/lhr, m-hmr (ONLY X) = VIABLE
D. sim = s-lhr, hmr (X) x (a) D. mel = lhr, m-hmr(X)/hmr (X)
1/2 SONS GET: s-lhr/lhr, hmr (only X) = VIABLE
B. Hybrid Incompatibility
D. melanogaster and D. simulans
SYSTEM: (s-lhr dominant)
Ancestor: lhr, mhr
D. simulans: s-lhr, mhr
D. melanogaster: lhr, m-mhr
s-lhr, m-mhr = INVIABLE
B. Hybrid Incompatibility
D. melanogaster and D. simulans
Brideau et al. 2006. Science 314: 1292-1295
- isolated location of lhr gene.
- put NORMAL D. simulans gene into D. melanogaster.
- mated these D. melanogaster with Watanabe's mutant strain of D.
simulans.
- IF these two genes are partners, then 3/4 hybrids should die.
(w) D. sim = lhr/s-lhr, hmr (X)
x (b)D. mel = s-lhr/lhr, m-hmr (X)
(doesn't die....)
1/4 SONS GET : lhr/lhr, m-hmr (only X) .... VIABLE
3/4 get some other combination including s-lhr and m-hmr.. INVIABLE
Speciation
I. Modes
II. Mechanisms
A. Progressive Genomic Incompatibility
B. Hybrid Incompatibility
C. Differential Selection
C. Differential Selection
- Assumed to be primary, but few studies documenting that
reproductive isolation of phenotypes correlates with fitness differential
in different environments.
Rundle et al. (2000). Science 287:306.
C. Differential Selection
- Assumed to be primary, but few studies documenting that
reproductive isolation of phenotypes correlates with fitness differential
in different environments.
Rundle et al. (2000). Science 287:306.
Sticklebacks colonizing lakes...PHYLOGENY:
limnetic
benthic
limnetic
benthic
limnetic
benthic
C. Differential Selection
- Assumed to be primary, but few studies documenting that
reproductive isolation of phenotypes correlates with fitness differential
in different environments.
Rundle et al. (2000). Science 287:306.
Mate selection
correlates with
ecotype, not with
genetic relatedness....
example of parallel
evolution, too.
Speciation
I. Modes
II. Mechanisms
A. Progressive Genomic Incompatibility
B. Hybrid Incompatibility
C. Differential Selection
D. Hybridization
D. Hybridization
- When hybridization occurs, it show increase gene flow between
populations. How are hybrids stabilized as a reproductively isolated
group?
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
Two species of small western butterflies
have overlapping ranges.
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
Two cluster
Three cluster
Probabilities of assigning individuals from these
populations to a particular dendrogram "cluster"
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
Two cluster
Three cluster
Probabilities of assigning individuals from these
populations to a particular dendrogram "cluster"
Are the alpine populations
simply in hybrid zone, or
are they reproductively
isolated?
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
Two cluster
Three cluster
Probabilities of assigning individuals from these
populations to a particular dendrogram "cluster"
Are the alpine populations
simply in hybrid zone, or
are they reproductively
isolated?
They are fixed for several
alleles, suggesting no
gene flow.
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
Two cluster
Three cluster
Probabilities of assigning individuals from these
populations to a particular dendrogram "cluster"
Are the alpine populations
simply in hybrid zone, or
are they reproductively
isolated?
They are fixed for several
alleles, suggesting no
gene flow.
- Also used coalescence
to estimate time since a
common ancestor within
each 'species". The alpine
populations had a more
recent history (400,000
yrs) than either of the
others (1.2-1.9 my)
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
- What maintains this genetic uniqueness?
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
- What maintains this genetic uniqueness? Fidelity to Host Plant
- adaptation to an extreme habitat
Gompert et al. 2006. Science 314: 1923.
- What maintains this genetic uniqueness?
Fidelity to Host Plant
Also, their eggs don't stick to the leaf; they drop off into litter.
This may be adaptive, as winds blow leaves a long way from
original plant at high elevations. The host plant is a perennial, so
dropping into the leaf litter keeps it close to host plant.
Other species, even if they used the plant, would have eggs dispersed
from the host plant. That's bad for butterflies, 'cuz caterpillars don't
disperse too far...
D. Hybridization
- When hybridization occurs, it show increase gene flow between
populations. How are hybrids stabilized as a reproductively isolated
group?
- adaptation to extreme habitat
- sexual selection
- sexual selection
Mavarez et al. 2006. Nature 441:868
X
BACKCROSS
BACKCROSS
Mating probabilities in no-choice experiments:
strong Positive Assortative Mating
female
Male
H. mel
H. heur
H. cyn
H. mel
1.00
0.07
0.18
H. heur
0.10
1.00
0.44
H. cyn
0.12
0.02
1.00
Mate Pairing in Tetrads:
strong Positive Assortative Mating
Speciation
I. Modes
II. Mechanisms
A. Progressive Genomic Incompatibility
B. Hybrid Incompatibility
C. Differential Selection
D. Hybridization
Several ways that new gene combinations
can form and become stabilized.
(C) The distribution across markers of the proportion of H. petiolaris alleles seen in experimental hybrids. There were three
generations of crossing within the hybrid population, followed by two generations of backcrossing to H. annuus. Therefore, in
the absence of selection, one expects 1/8 of the genes to derive from H. petiolaris, with a distribution concentrated in the 1–
25% class. In regions of genome with the same gene order in H. petiolaris and H. annuus (red ), most markers fail to introgress,
but some introgress more than expected. In regions of genome that differ in gene order as a result of chromosome
rearrangements, there is almost no introgression (blue). (D) Patterns of introgression along the genomes are similar between
experimental hybrids and the natural hybrid species, H. anomalus. Three of the 17 H. anomalus chromosomes are shown. The
letters to the left (R, S, T, Q) indicate homology of these chromosomes to regions of the parental genomes. (The leftmost
chromosome is rearranged, and combines linkage blocks R and S.) Arrows to the right indicate the genetic markers. The
shading indicates the likelihood that the regions derived from H. annuus (blue) or H. petiolaris (yellow). (A, Courtesy USDA; B,
redrawn from Rogers et al. 1982; C, data from Table 1 in Rieseberg et al. 1995a; D, redrawn from Fig. 3 in Rieseberg and
Noyes 1998.)