Download IR1. The ecophysiological mechanisms for salt and drought stress

Ecophysiological characterization of Coccoloba uvifera
L.: the case of a salt and drought stress tolerant plant
species for sand dune reclamation in Cuba
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
Overall Academic Objective (OAO): strengthen research and training on abiotic,
especially salt and drought, stress of economically interesting species at Granma
University (GU).
Overall Developmental Objective (ODO): improve capacity building for research
in salt and drought stress at GU.
Specific Academic Objective (SAO): strengthen research and training on
ecophysiological, nutritional and phyto-chemical characterization and fruit
production under salt and drought stress of C. uvifera in saline coastal soils of
Cuba.
Specific Developmental Objective (SDO): develop the capacities for
ecophysiologically trained researchers to reduce climate change vulnerability of
the coastal areas of Cuba.
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
Intermediate Results (IR)
IR1. The ecophysiological mechanisms for salt and drought stress tolerance of C. uvifera
are known.
IR2. Nutritional and phytochemical compounds of C. uvifera are characterized and known.
IR3. The visibility of the Abiotic Stress Group of the Biotechnology Study Centre, as an
excellence center for ecophysiological salt and drought stress research in Cuba has
increased
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
IR1.
The
ecophysiological
mechanisms for salt
and drought stress
tolerance of C. uvifera
are known.
1.1.
Preparation
of experiments
1.1.2
Literature review
about drought and salt stress in
relation to the selected and
similar species
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
IR1. The ecophysiological
mechanisms for salt and
drought stress tolerance
of C. uvifera are known.
1.1.
Preparation
of
experiments
North
South
Playa Guardalavaca,
Holguin province
Latitude: 21°07′30″N
Longitude: 75°49′44″O
East
Playa Las Coloradas,
Granma province
Latitude: 19° 56' 00" N
Longitude: 77° 41' 00" W
1.1.3
Definition
of
experimental design and practical
requirements
Playa Ancon, Sancti Spiritus
province
Latitude: 21° 44' 00" N
Longitude: 80° 01' 00" W
Middle
Cayo Coco, Ciego de Avila
province
Latitude: 22º 32' 10.06" N
Longitude: 78º 21' 19.44" W
West
Playa Varadero, Matanzas
province
Latitude: 23°10'28"N
Longitude: 81°12'43"W
Playa Rancho Luna,
Cienfuegos province
Latitude: 22°02′20″N
Longitude: 80°25′38″W
IR1. The ecophysiological
mechanisms for salt and
drought stress tolerance
of C. uvifera are known.
1.1.
Preparation
of
experiments
1.1.3
Definition
of
experimental design and practical
requirements
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
IR1.
The
ecophysiological
mechanisms for salt and
drought stress tolerance of C.
uvifera are known.
1.1.
Preparation
of
experiments
1.1.5
Training of the personnel
responsible for the manipulation of the
equipment and the measurements
IR1.
The
ecophysiological
mechanisms for salt and
drought stress tolerance of C.
uvifera are known.
1.2.
Experiments
in
controlled conditions
1.2.1 Evaluation of the response to
salt and drought stress of C. uvifera
during germination
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
IR1.
The
ecophysiological
mechanisms for salt and
drought stress tolerance of C.
uvifera are known.
Control
5 dS.m-1
1.2.
Experiments
in
controlled conditions
1.2.1 Evaluation of the response to
salt and drought stress of C. uvifera
during germination
15 dS.m-1
Salt stress
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
25 dS.m-1
100 % field
capacity
IR1.
The
ecophysiological
mechanisms for salt and
drought stress tolerance of C.
uvifera are known.
1.2.
Experiments
in
controlled conditions
1.2.1 Evaluation of the response to
salt and drought stress of C. uvifera
during germination
Drought stress
0% field
capacity
50 % field
capacity
IR1.
The
ecophysiological
mechanisms for salt and
drought stress tolerance of C.
uvifera are known.
1.2.
Experiments
in
controlled conditions
1.2.2 Evaluation of ecophysiological
responses
(gas
exchange,
photosynthesis,
chlorophyll
fluorescence, plant-water-relations,
leaf area index) of C. uvifera to salt
and drought stress at seedling and
sapling stage.
Salt stress
Control
15 dS.m-1
IR1.
The
ecophysiological
mechanisms for salt and
drought stress tolerance of C.
uvifera are known.
Control
15 dS.m-1
1.2.
Experiments
in
controlled conditions
1.2.2 Evaluation of ecophysiological
responses
(gas
exchange,
photosynthesis,
chlorophyll
fluorescence, plant-water-relations,
leaf area index) of C. uvifera to salt
and drought stress at seedling and
sapling stage.
Salt stress
Control
15 dS.m-1
IR1.
The
ecophysiological
mechanisms for salt and
drought stress tolerance of C.
uvifera are known.
1.2.
Experiments
in
controlled conditions
1.2.2 Evaluation of ecophysiological
responses
(gas
exchange,
photosynthesis,
chlorophyll
fluorescence, plant-water-relations,
leaf area index) of C. uvifera to salt
and drought stress at seedling and
sapling stage.
Salt stress
North
1.3. Experiments in field
conditions.
1.3.2 Fruit collection of C.
uvifera accessions in
selected ecosystems.
South
Playa Guardalavaca,
Holguin province
Latitude: 21°07′30″N
Longitude: 75°49′44″O
East
Playa Las Coloradas,
Granma province
Latitude: 19° 56' 00" N
Longitude: 77° 41' 00" W
IR2.
Nutritional
and
phytochemical compounds
of
C.
uvifera
are
characterized and known.
2.1 Intensive literature
review on C. uvifera about
the
nutritional
and
medicinal compounds
IR2.
Nutritional
and
phytochemical compounds
of
C.
uvifera
are
characterized and known.
2.2 Evaluation of nutritional
quality of C. uvifera
2.3
Phytochemical
screening and antibacterial
activity evaluation of C.
uvifera
Metabolitos
Alcaloides
Coumarinas
Ácidos grasos
Resinas
Triterpenos y/o esteroides
Saponinas
Aminoácidos libres
Glicósidos cardiotónicos
Carbohidratos reductores
Fenoles y/o taninos
Quinonas
Antocianidinas
Flavonoides
Principios amargos
Mucílagos
Cabo Cruz
Pilón
Manzanillo
Hojas
Corteza del tallo
Fruto
Hojas
Corteza del tallo
Fruto
Hojas
Corteza del tallo
Fruto
Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac Dcm Et Ac
- + - +++ - +++ - - +++ - +++ - +++ - - +++ - +++ - +++ ++ ++
++ ++
++ ++
+ ++
++ ++
++ ++
+ ++
++ ++
++ ++
+
+
+
++
+
+
-
-
+
+
-
+
++
+
+
-
+
+
+
-
Metabolites
DCM
Alkaloids
Coumarins
Fatty Acid
Resins
Triterpenes/Steroids
Saponines* (ultrasonido)
Saponines* (maceración)
Free amino acid
Cardiotonic Glycosides
Reducers Carbohydrates
Phenols/Tanines
Quinones
Antocianidines
Flavonoids
Better compounds
Mucilages
+
+
-
+
++
+
+
-
+
+
++
+
-
+
+
+
+
-
Leaves
EtOH EtOAc
+
++
-*
+*
++
+++
+
+
-
+
+
+++
+
-
+
+
+
-
+
+
-
- +
++ +
+ +
+ +++
+
-
+
+
+
+
-
North of east region
Bark of the shaft
DCM
EtOH EtOAc
++
+
-
++
++
+
+++
+
+
-
+
+
++
-
+
++
+
+
+
+
+
+
+
+
+
+
+
+
-
+
+
+
+
-
+
++
+
+
+
+
+
++
+
-
DCM
Fruits
EtOH
EtOAc
+
+
+
-
++
++
-*
+*
+
++
+++
+
+
-
+
++
++
-
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
Prof.Dr. Raúl Carlos López Sánchez
Local promoter
Prof.Dr. Raúl Carlos López Sánchez
Local promoter