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Glycolysis and Cellular Respiration Go to Section: Slide # 2 Cells Need Energy 1. Organisms obtain energy from food 2. Glucose is main source of food for cells 3. Cells break chemical bonds of glucose; energy released 4. Energy is stored in ATP 5. Making ATP is part of cellular respiration Go to Section: Slide # 3 Cellular Respiration Equation Cellular Respiration produces carbon dioxide and water, which are used as starting materials (reactants) in photosynthesis Enzymes C6H12O6 + 6 O2 6 CO2 + 6 H2O + 36 ATP Reactants Products Slide # 4 Overview of Cellular Respiration Pathways Glucose Glycolysis Krebs cycle Fermentation (without oxygen) Go to Section: Electron transport Alcohol or lactic acid Slide # 5 Glycolysis: Breaking Sugar 1. Occurs in cytoplasm 2. Glucose (C6H12O6) is broken down into 2 molecules of pyruvic acid (C3H6O3) 3. Does not use oxygen (anaerobic) 4. Net gain of 2 ATP’s 4 ATP produced — 2 ATP consumed 2 ATP net gain Also produces: 2 NADH 2 pyruvic acids Glycolysis occurs in prokaryotes & eukaryotes An overview of the process of glycolysis. Cellular Respiration: The Krebs Cycle & Electron Transport Go to Section: Slide # 7 Cellular Respiration 1. Cellular respiration: process that releases energy by breaking the chemical bonds of glucose in the presence of oxygen. a. Aerobic b/c it uses oxygen b. Occurs in the mitochondria c. Occurs in eukaryotic cells such as: Go to Section: Slide # 8 After Glycolysis: Cellular Respiration 1. About 90% of energy from glucose remains in pyruvic acid 2. Purpose of the Krebs cycle: a. Release the remaining energy & capture the highly energized electrons by NAD+ & FAD (electron carriers) 3. Produces CO2 as waste cytoplasm Slide # 9 The Breakdown of Pyruvic Acid 1. Pyruvic acid diffuses into matrix of the mitochondria 2. Each pyruvic acid is converted into acetyl-CoA a. Produces 2 CO2 b. Produces 2 NADH c. Transfers hydrogen atoms & highly energized electrons to NAD+ & FAD Slide # 10 Results of the Krebs Cycle Break down of Acetyl-CoA yields: 1 Acetyl-CoA 2 Acetyl-CoA 1 ATP 3 CO2 4 NADH 1 FADH2 2 ATP 6 CO2 8 NADH 2 FADH2 ATP and CO2 diffuse out of the mitochondria NADH and FADH2 move to the cristae Slide # 11 ETC: Oxidative Phosphorylation 1. Uses the H (carried by NADH & FADH2) to build up a H+ gradient in the inner membrane space to make 32 ATPs 2. Oxygen is consumed & water produced 3. ADP is phosphorylated (to ATP) in the presence of O2 Inner Membrane Space Slide # 12 The Electron Transport Chain 1. The ETC is located on inner membrane of the mitochondria. (cristae) 2. Oxygen is final acceptor of electrons. 3. Produces 32 ATP’s. a. the most efficient form of ATP production Proton Gradient builds up on one side of the membrane Slide # 13 The Electron Transport Chain 1. NADH & FADH2 carry pairs of electrons (from glycolysis & Krebs cycle) to ETC. 2. As electrons are passed along ETC, the energy in the electrons is used to pump H+ into inter membrane space. a. Creates a high concentration of H+ in inter membrane space b. H atoms diffuse back into core of mitochondria through transport proteins (chemiosmosis) c. Transport proteins use flow of H atoms to bond last P onto ADP to create ATP (phosphorylation) Fermentation: Anaerobic My muscles are on fire! 1. Respiration that does not use O2 2. Releases net gain of 2ATP’s 3. Most of the energy from glucose remains in the organic molecules (lactic acid or alcohol) produced from fermentation. Lactic Acid fermentation: a. Glucose is broken down into lactic acid b. Used to produce yogurt, buttermilk and sour cream c. Occurs in muscle cells during intense exercise d. Lactic acid causes muscle cells to BURN Alcoholic fermentation: (Occurs in Yeast) a. Glucose is broken down into alcohol & CO2 b. CO2 given off by yeast causes bread dough rise c. Process is also used to make biofuels out of crop residue Slide # 15 Sequence these 3 1 2 Oxidative phosphorylation Glycolysis Krebs cycle