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LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson Chapter 3 Water and Life Lectures by Erin Barley Kathleen Fitzpatrick © 2011 Pearson Education, Inc. Overview: The Molecule That Supports All of Life • Water is the biological medium on Earth • All living organisms require water more than any other substance • Most cells are surrounded by water, and cells themselves are about 70–95% water • The abundance of water is the main reason the Earth is habitable © 2011 Pearson Education, Inc. Concept 3.2: Four emergent properties of water contribute to Earth’s suitability for life • Four of water’s properties that facilitate an environment for life are – Cohesive behavior – Ability to moderate temperature – Expansion upon freezing – Versatility as a solvent © 2011 Pearson Education, Inc. Cohesion of Water Molecules • Collectively, hydrogen bonds hold water molecules together, a phenomenon called cohesion (Capillary action- the transport of water against gravity in plants) • Adhesion is an attraction between different substances, for example, between water and plant cell walls © 2011 Pearson Education, Inc. Animation: Water Transport Right-click slide/select “Play” © 2011 Pearson Education, Inc. • Surface tension is a measure of how hard it is to break the surface of a liquid • Surface tension is related to cohesion © 2011 Pearson Education, Inc. Moderation of Temperature by Water • Water absorbs heat from warmer air and releases stored heat to cooler air • Water can absorb or release a large amount of heat with only a slight change in its own temperature • Ocean as climate moderator of Earth © 2011 Pearson Education, Inc. Heat and Temperature • Kinetic energy is the energy of motion • Heat is a measure of the total amount of kinetic energy due to molecular motion • Temperature measures the intensity of heat due to the average kinetic energy of molecules © 2011 Pearson Education, Inc. • The Celsius scale is a measure of temperature using Celsius degrees (°C) • A calorie (cal) is the amount of heat required to raise the temperature of 1 g of water by 1°C • The “calories” on food packages are actually kilocalories (kcal), where 1 kcal = 1,000 cal • The joule (J) is another unit of energy where 1 J = 0.239 cal, or 1 cal = 4.184 J © 2011 Pearson Education, Inc. Water’s High Specific Heat • The specific heat of a substance is the amount of heat that must be absorbed or lost for 1 g of that substance to change its temperature by 1ºC • The specific heat of water is 1 cal/g/ºC • Water resists changing its temperature because of its high specific heat © 2011 Pearson Education, Inc. • Water’s high specific heat can be traced to hydrogen bonding – Heat is absorbed when hydrogen bonds break – Heat is released when hydrogen bonds form • The high specific heat of water minimizes temperature fluctuations to within limits that permit life © 2011 Pearson Education, Inc. Figure 3.5 Los Angeles (Airport) 75° 70s (°F) 80s San Bernardino 100° Riverside 96° Santa Ana Palm Springs 84° 106° Burbank 90° Santa Barbara 73° Pacific Ocean 68° 90s 100s San Diego 72° 40 miles Evaporative Cooling • Evaporation is transformation of a substance from liquid to gas • Heat of vaporization is the heat a liquid must absorb for 1 g to be converted to gas • As a liquid evaporates, its remaining surface cools, a process called evaporative cooling • Evaporative cooling of water helps stabilize temperatures in organisms and bodies of water © 2011 Pearson Education, Inc. Floating of Ice on Liquid Water • Ice floats in liquid water because hydrogen bonds in ice are more “ordered,” making ice less dense • Water reaches its greatest density at 4°C • If ice sank, all bodies of water would eventually freeze solid, making life impossible on Earth © 2011 Pearson Education, Inc. Figure 3.6 Hydrogen bond Ice: Hydrogen bonds are stable Liquid water: Hydrogen bonds break and re-form Water: The Solvent of Life • A solution is a liquid that is a homogeneous mixture of substances • A solvent is the dissolving agent of a solution • The solute is the substance that is dissolved • An aqueous solution is one in which water is the solvent • Coffee and sugar © 2011 Pearson Education, Inc. • Water can also dissolve compounds made of nonionic polar molecules • Even large polar molecules such as proteins can dissolve in water if they have ionic and polar regions © 2011 Pearson Education, Inc. Hydrophilic and Hydrophobic Substances • A hydrophilic substance is one that has an affinity for water • A hydrophobic substance is one that does not have an affinity for water • Oil molecules are hydrophobic because they have relatively nonpolar bonds • A colloid is a stable suspension of fine particles in a liquid © 2011 Pearson Education, Inc. Solute Concentration in Aqueous Solutions • Most biochemical reactions occur in water • Chemical reactions depend on collisions of molecules and therefore on the concentration of solutes in an aqueous solution © 2011 Pearson Education, Inc. Concept 3.3: Acidic and basic conditions affect living organisms • A hydrogen atom in a hydrogen bond between two water molecules can shift from one to the other – The hydrogen atom leaves its electron behind and is transferred as a proton, or hydrogen ion (H+) – The molecule with the extra proton is now a hydronium ion (H3O+), though it is often represented as H+ – The molecule that lost the proton is now a hydroxide ion (OH–) © 2011 Pearson Education, Inc. Figure 3.UN02 + 2 H 2O Hydronium ion (H3O+) Hydroxide ion (OH) • Though statistically rare, the dissociation of water molecules has a great effect on organisms • Changes in concentrations of H+ and OH– can drastically affect the chemistry of a cell © 2011 Pearson Education, Inc. • Concentrations of H+ and OH– are equal in pure water • Adding certain solutes, called acids and bases, modifies the concentrations of H+ and OH– • Biologists use something called the pH scale to describe whether a solution is acidic or basic (the opposite of acidic) © 2011 Pearson Education, Inc. Acids and Bases • An acid is any substance that increases the H+ concentration of a solution • A base is any substance that reduces the H+ concentration of a solution © 2011 Pearson Education, Inc. The pH Scale • In any aqueous solution at 25°C the product of H+ and OH– is constant and can be written as [H+][OH–] = 10–14 • The pH of a solution is defined by the negative logarithm of H+ concentration, written as pH = –log [H+] • For a neutral aqueous solution, [H+] is 10–7, so pH = –(–7) = 7 © 2011 Pearson Education, Inc. Figure 3.10 H+ H+ H+ H+ OH + OH H H+ + H H+ Acidic solution Increasingly Acidic [H+] > [OH] pH Scale 0 1 Battery acid 2 Gastric juice, lemon juice 3 Vinegar, wine, cola 4 Tomato juice Beer Black coffee 5 6 Neutral solution OH OH OH H+ OH OH OH OH + H Basic solution Neutral [H+] = [OH] 7 8 Increasingly Basic [H+] < [OH] OH OH H+ H+ OH OH OH + H+ H+ H Rainwater Urine Saliva Pure water Human blood, tears Seawater Inside of small intestine 9 10 Milk of magnesia 11 Household ammonia 12 13 Household bleach Oven cleaner 14 Buffers • The internal pH of most living cells must remain close to pH 7 • Buffers are substances that minimize changes in concentrations of H+ and OH– in a solution • Most buffers consist of an acid-base pair that reversibly combines with H+ © 2011 Pearson Education, Inc. Acidification: A Threat to Water Quality • Human activities such as burning fossil fuels threaten water quality • CO2 is the main product of fossil fuel combustion • About 25% of human-generated CO2 is absorbed by the oceans • CO2 dissolved in sea water forms carbonic acid; this process is called ocean acidification © 2011 Pearson Education, Inc. Figure 3.11 CO2 CO2 + H2O H2CO3 H2CO3 H+ + HCO3 H+ + CO32 CO32 + Ca2+ HCO3 CaCO3 • As seawater acidifies, H+ ions combine with carbonate ions to produce bicarbonate • Carbonate is required for calcification (production of calcium carbonate) by many marine organisms, including reef-building corals © 2011 Pearson Education, Inc. • The burning of fossil fuels is also a major source of sulfur oxides and nitrogen oxides • These compounds react with water in the air to form strong acids that fall in rain or snow • Acid precipitation is rain, fog, or snow with a pH lower than 5.2 • Acid precipitation damages life in lakes and streams and changes soil chemistry on land © 2011 Pearson Education, Inc. LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson Chapter 3 Water and Life Questions prepared by Ruth Buskirk University of Texas at Austin Lectures by Jung Choi Erin Barley Georgia Institute of Technology Kathleen Fitzpatrick © 2011 Pearson Education, Inc. The four emergent properties of water that are important for life are: a) Cohesion, expansion upon freezing, high heat of evaporation, and capillarity b) Cohesion, moderation of temperature, expansion upon freezing, and solvent properties c) Moderation of temperature, solvent properties, high surface tension, and capillarity d) Heat of vaporization, high specific heat, high surface tension, and capillarity e) Polarity, hydrogen bonding, high specific heat, and high surface tension The four emergent properties of water that are important for life are: a) Cohesion, expansion upon freezing, high heat of evaporation, and capillarity b) Cohesion, moderation of temperature, expansion upon freezing, and solvent properties c) Moderation of temperature, solvent properties, high surface tension, and capillarity d) Heat of vaporization, high specific heat, high surface tension, and capillarity e) Polarity, hydrogen bonding, high specific heat, and high surface tension Water has an unusually high specific heat. This is directly related to which one of the following? – At its boiling point, water changes from liquid to vapor. – More heat is required to raise the temperature of water. – Ice floats in liquid water. – Salt water freezes at a lower temperature than pure water. – Floating ice can insulate bodies of water. Water has an unusually high specific heat. This is directly related to which one of the following? – At its boiling point, water changes from liquid to vapor. – More heat is required to raise the temperature of water. – Ice floats in liquid water. – Salt water freezes at a lower temperature than pure water. – Floating ice can insulate bodies of water. Surfactants reduce surface tension of a liquid. Which of the following would result if water was treated with surfactants? a) Surfactant-treated water droplets will form a thin film instead of beading on a waxed surface. b) Surfactant-treated water will form smaller droplets when dripping from a sink. c) Water striders will sink. d) All of the above will occur e) Only a and c will occur. Surfactants reduce surface tension of a liquid. Which of the following would result if water was treated with surfactants? a) Surfactant-treated water droplets will form a thin film instead of beading on a waxed surface. b) Surfactant-treated water will form smaller droplets when dripping from a sink. c) Water striders will sink. d) All of the above will occur e) Only a and c will occur. A calorie is defined as: a) The amount of energy required to raise the temperature of 1 gram of water by 1 degree F. b) The amount of energy required to raise the temperature of 1 gram of water by 1 degree C. c) The amount of energy released by metabolism of one gram of glucose. d) The amount of energy contained in one gram of fat. e) The amount of energy required to raise the temperature of 1 liter of water by 1 degree C. A calorie is defined as: a) The amount of energy required to raise the temperature of 1 gram of water by 1 degree F. b) The amount of energy required to raise the temperature of 1 gram of water by 1 degree C. c) The amount of energy released by metabolism of one gram of glucose. d) The amount of energy contained in one gram of fat. e) The amount of energy required to raise the temperature of 1 liter of water by 1 degree C. In humans, blood pH is around 7.4, and a decrease in blood pH to 6.4 would be fatal. A drop by 1 pH unit represents which of these? a) b) c) d) e) 1/10 as many H+ ions in the solution 1/7 as many H+ ions in the solution 1/2 as many H+ ions in the solution twice as many H+ ions in the solution ten times as many H+ ions in the solution In humans, blood pH is around 7.4, and a decrease in blood pH to 6.4 would be fatal. A drop by 1 pH unit represents which of these? a) b) c) d) e) 1/10 as many H+ ions in the solution 1/7 as many H+ ions in the solution 1/2 as many H+ ions in the solution twice as many H+ ions in the solution ten times as many H+ ions in the solution The chemical equilibrium between carbonic acid and bicarbonate acts as a pH regulator in our blood. If the blood pH begins to rise, what will happen? H2CO3 ↔ HCO3– + H+ Carbonic acid Bicarbonate ion Hydrogen ion a) reaction proceeds to the right; more carbonic acid dissociates b) reaction proceeds to the right; more carbonic acid forms c) reaction proceeds to the left; more carbonic acid dissociates d) reaction proceeds to the left; more carbonic acid forms The chemical equilibrium between carbonic acid and bicarbonate acts as a pH regulator in our blood. If the blood pH begins to rise, what will happen? H2CO3 ↔ HCO3– + H+ Carbonic acid Bicarbonate ion Hydrogen ion a) reaction proceeds to the right; more carbonic acid dissociates b) reaction proceeds to the right; more carbonic acid forms c) reaction proceeds to the left; more carbonic acid dissociates d) reaction proceeds to the left; more carbonic acid forms Scientists are concerned about increased concentrations of atmospheric carbon dioxide due to increased fossil fuel combustion and deforestation. In addition to major effects on global temperatures, increased levels of CO2 can threaten aquatic organisms in which way? a) forming more carbonic acid in a solution that raises the pH of seawater b) forming more carbonic acid that decreases the concentration of carbonate ions in seawater c) increasing photosynthesis rates in aquatic plants and algae d) decreasing the oxygen available for cellular respiration in aquatic organisms e) increasing the levels of harmful sulfur oxides and nitrous oxides Scientists are concerned about increased concentrations of atmospheric carbon dioxide due to increased fossil fuel combustion and deforestation. In addition to major effects on global temperatures, increased levels of CO2 can threaten aquatic organisms in which way? a) forming more carbonic acid in a solution that raises the pH of seawater b) forming more carbonic acid that decreases the concentration of carbonate ions in seawater c) increasing photosynthesis rates in aquatic plants and algae d) decreasing the oxygen available for cellular respiration in aquatic organisms e) increasing the levels of harmful sulfur oxides and nitrous oxides LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson Chapter 4 Carbon and the Molecular Diversity of Life Lectures by Erin Barley Kathleen Fitzpatrick © 2011 Pearson Education, Inc. Overview: Carbon: The Backbone of Life • Living organisms consist mostly of carbon-based compounds (ORGANIC) • Organic chemistry is the study of compounds that contain carbon • Organic compounds range from simple molecules to colossal ones • Most organic compounds contain hydrogen atoms in addition to carbon atoms © 2011 Pearson Education, Inc. • Vitalism, the idea that organic compounds arise only in organisms, was disproved when chemists synthesized these compounds • Mechanism is the view that all natural phenomena are governed by physical and chemical laws © 2011 Pearson Education, Inc. Organic Molecules and the Origin of Life on Earth • Stanley Miller’s classic experiment demonstrated the abiotic synthesis of organic compounds • Experiments support the idea that abiotic synthesis of organic compounds, perhaps near volcanoes, could have been a stage in the origin of life © 2011 Pearson Education, Inc. Figure 4.2 EXPERIMENT “Atmosphere” CH4 Water vapor Electrode Condenser Cooled “rain” containing organic molecules H2O “sea” Sample for chemical analysis Cold water Concept 4.2: Carbon atoms can form diverse molecules by bonding to four other atoms • Electron configuration is the key to an atom’s characteristics • Electron configuration determines the kinds and number of bonds an atom will form with other atoms © 2011 Pearson Education, Inc. The Formation of Bonds with Carbon • With four valence electrons, carbon can form four covalent bonds with a variety of atoms ability makes large, complex molecules • In molecules with multiple carbons, each carbon bonded to four other atoms has a tetrahedral shape • However, when two carbon atoms are joined by a double bond, the atoms joined to the carbons are in the same plane as the carbons © 2011 Pearson Education, Inc. Figure 4.3 Name and Comment Molecular Formula (a) Methane CH4 (b) Ethane C2H6 (c) Ethene (ethylene) C2H4 Structural Formula Ball-andStick Model Space-Filling Model Figure 4.4 Hydrogen (valence 1) Oxygen (valence 2) Nitrogen (valence 3) Carbon (valence 4) Figure 4.5 (c) Double bond position (a) Length Ethane Propane (b) Branching Butane 1-Butene 2-Butene (d) Presence of rings 2-Methylpropane (isobutane) Cyclohexane Benzene Hydrocarbons • Hydrocarbons are organic molecules consisting of only carbon and hydrogen • Many organic molecules, such as fats, have hydrocarbon components • Hydrocarbons can undergo reactions that release a large amount of energy © 2011 Pearson Education, Inc. Figure 4.6 Nucleus Fat droplets 10 m (a) Part of a human adipose cell (b) A fat molecule Isomers • Isomers are compounds with the same molecular formula but different structures and properties – Structural isomers have different covalent arrangements of their atoms – Cis-trans isomers have the same covalent bonds but differ in spatial arrangements – Enantiomers are isomers that are mirror images of each other © 2011 Pearson Education, Inc. Animation: Isomers Right-click slide / select “Play” © 2011 Pearson Education, Inc. Figure 4.7 (a) Structural isomers (b) Cis-trans isomers cis isomer: The two Xs are on the same side. trans isomer: The two Xs are on opposite sides. (c) Enantiomers CO2H CO2H H NH2 CH3 L isomer NH2 H CH3 D isomer • Enantiomers are important in the pharmaceutical industry- Dopamine • Two enantiomers of a drug may have different effects • Usually only one isomer is biologically active • Differing effects of enantiomers demonstrate that organisms are sensitive to even subtle variations in molecules © 2011 Pearson Education, Inc. Animation: L-Dopa Right-click slide / select “Play” © 2011 Pearson Education, Inc. Figure 4.8 Drug Condition Ibuprofen Pain; inflammation Albuterol Effective Enantiomer Ineffective Enantiomer S-Ibuprofen R-Ibuprofen R-Albuterol S-Albuterol Asthma Concept 4.3: A few chemical groups are key to the functioning of biological molecules • Distinctive properties of organic molecules depend on the carbon skeleton and on the molecular components attached to it • A number of characteristic groups can replace the hydrogens attached to skeletons of organic molecules © 2011 Pearson Education, Inc. The Chemical Groups Most Important in the Processes of Life • Functional groups are the components of organic molecules that are most commonly involved in chemical reactions • The number and arrangement of functional groups give each molecule its unique properties © 2011 Pearson Education, Inc. Figure 4.UN02 Estradiol Testosterone • The seven functional groups that are most important in the chemistry of life: – – – – – – – Hydroxyl group Carbonyl group Carboxyl group Amino group Sulfhydryl group Phosphate group Methyl group © 2011 Pearson Education, Inc. Figure 4.9-a CHEMICAL GROUP Hydroxyl Carbonyl Carboxyl STRUCTURE (may be written HO—) NAME OF COMPOUND Alcohols (Their specific names usually end in -ol.) Ketones if the carbonyl group is within a carbon skeleton Carboxylic acids, or organic acids Aldehydes if the carbonyl group is at the end of the carbon skeleton EXAMPLE Ethanol Acetone Acetic acid Propanal FUNCTIONAL PROPERTIES • Is polar as a result of the electrons spending more time near the electronegative oxygen atom. • Can form hydrogen bonds with water molecules, helping dissolve organic compounds such as sugars. • A ketone and an aldehyde may be structural isomers with different properties, as is the case for acetone and propanal. • Ketone and aldehyde groups are also found in sugars, giving rise to two major groups of sugars: ketoses (containing ketone groups) and aldoses (containing aldehyde groups). • Acts as an acid; can donate an H+ because the covalent bond between oxygen and hydrogen is so polar: Nonionized Ionized • Found in cells in the ionized form with a charge of 1 and called a carboxylate ion. Figure 4.9-b Amino Sulfhydryl Phosphate Methyl (may be written HS—) Amines Organic phosphates Thiols Cysteine Glycine • Acts as a base; can pick up an H+ from the surrounding solution (water, in living organisms): Nonionized Ionized • Found in cells in the ionized form with a charge of 1+. Glycerol phosphate • Two sulfhydryl groups can react, forming a covalent bond. This “cross-linking” helps stabilize protein structure. • Contributes negative charge to the molecule of which it is a part (2– when at the end of a molecule, as above; 1– when located internally in a chain of phosphates). • Cross-linking of cysteines in hair proteins maintains the curliness or straightness of hair. Straight hair can be “permanently” curled by shaping it around curlers and then breaking and re-forming the cross-linking bonds. • Molecules containing phosphate groups have the potential to react with water, releasing energy. Methylated compounds 5-Methyl cytidine • Addition of a methyl group to DNA, or to molecules bound to DNA, affects the expression of genes. • Arrangement of methyl groups in male and female sex hormones affects their shape and function. ATP: An Important Source of Energy for Cellular Processes • One phosphate molecule, adenosine triphosphate (ATP), is the primary energytransferring molecule in the cell • ATP consists of an organic molecule called adenosine attached to a string of three phosphate groups © 2011 Pearson Education, Inc. Figure 4. UN04 Adenosine Figure 4. UN05 Reacts with H2O Adenosine Adenosine ATP Inorganic phosphate ADP Energy The Chemical Elements of Life: A Review • The versatility of carbon makes possible the great diversity of organic molecules • Variation at the molecular level lies at the foundation of all biological diversity © 2011 Pearson Education, Inc. LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson Chapter 4 Carbon and the Molecular Diversity of Life Questions prepared by William Wischusen Louisiana State University Lectures by Jung Choi Erin Barley Georgia Institute of Technology Kathleen Fitzpatrick © 2011 Pearson Education, Inc. What functional group is commonly used in cells to transfer energy from one organic molecule to another? •a) carboxyl •b) sulfhydryl •c) hydroxyl •d) phosphate •e) amino What functional group is commonly used in cells to transfer energy from one organic molecule to another? •a) carboxyl •b) sulfhydryl •c) hydroxyl •d) phosphate •e) amino The Miller-Urey experiment, shown below, a) showed that the conditions of early Earth were inhospitable to life b) demonstrated that amino acids and other organic molecules form under conditions that may have existed on Earth before life began c) proved that life could originate from inorganic chemicals d) both B and C The Miller-Urey experiment, shown below, a) showed that the conditions of early Earth were inhospitable to life b) demonstrated that amino acids and other organic molecules form under conditions that may have existed on Earth before life began c) proved that life could originate from inorganic chemicals d) both B and C Based on carbon’s valence (4), how many different molecular shapes can be made from 6 carbons? – – – – – 1 2 4 6 more than 6 Based on carbon’s valence (4), how many different molecular shapes can be made from 6 carbons? – – – – – 1 2 4 6 more than 6 Given a chemical formula for an organic molecule, (e.g., C6H12O6), one can usually deduce its a) b) c) d) e) Structure Molecular weight Solubility in water All of the above Molecular weight and solubility in water Given a chemical formula for an organic molecule, (e.g., C6H12O6), one can usually deduce its a) b) c) d) e) Structure Molecular weight Solubility in water All of the above Molecular weight and solubility in water Is this molecule soluble in water? – yes – no Is this molecule soluble in water? – yes – no Compared to a carbon atom with only single covalent bonds, a carbon with a double bond a) Shares fewer electron pairs with other atoms b) Is more flexible in the spatial arrangement of its bonds c) Has a planar configuration of its electron orbitals rather than a tetrahedral configuration d) Is more polar Compared to a carbon atom with only single covalent bonds, a carbon with a double bond a) Shares fewer electron pairs with other atoms b) Is more flexible in the spatial arrangement of its bonds c) Has a planar configuration of its electron orbitals rather than a tetrahedral configuration d) Is more polar The general structure of amino acids are shown in this figure. What functional groups are highlighted in salmon and yellow, respectively? a) Amino and carboxyl b) Amino and carbonyl c) Hydroxyl and carbonyl d) Methyl and carboxyl e) Methyl and hydroxyl The general structure of amino acids are shown in this figure. What functional groups are highlighted in salmon and yellow, respectively? a) Amino and carboxyl b) Amino and carbonyl c) Hydroxyl and carbonyl d) Methyl and carboxyl e) Methyl and hydroxyl