Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Polydiacetylene Vesicles: Direct Biosensors with a Colorimetric Response Margaret A. Schmitt Samuel H. Gellman Group University of Wisconsin, Madison February 20, 2003 Outline Biosensors Definition and Introduction Direct and Indirect Polydiacetylenes Polymerization Reaction Chromic Response to Environmental Changes Harnessing Chromic Response in a Useful Biosensor Construct Variables Associated with Designing an Appropriate PDA Biosensor for a Variety of Systems Biosensors Device incorporating a biological sensing element directly connected to a signal transducer Biosensor design research attempts to couple Nature’s “lock-and-key” interactions with cleverly engineered signal transduction mechanisms Molecular recognition assumes many forms: enzyme-substrate, antibody-antigen, and receptorligand interactions Two-fold utility: Basic science level: develops an understanding of complex biological processes Applied science level: broad applicability in industrial and medicinal settings Analyte “recognition” Signal Biologically Sensitive Element X No interaction No signal Importance of Biosensing Techniques Glucose level monitoring in individuals with diabetes 1 3 2 4 http://www.healthchecksystems.com/bioscanner.htm Rapid detection of toxins and other biological warfare agents Response Designing Usable Biosensors Response Conc. Analyte Conc. Analyte Signal must have a direct relationship to quantity of material being analyzed Sensor must demonstrate specificity and selectivity in recognizing a single compound or group of compounds in a varied mixture Biosensor Detection Ranges -1 Detection limit of sensor must be within a relevant range Sensor must have a reasonable response time Glucose Cholesterol Metabolites: mM range -3 Log Concentration (1/mol) -2 -4 Iron -5 -6 Antibodies/Antigens: nM - pM range -7 -8 Syphilis Rubella -9 Rh Antigen -10 Hepatitis -11 -12 Indirect vs. Direct Biosensors Indirect: Relies on detection of a labeled ligand after a binding event has occurred. Direct: Binding event is directly linked to a signal transduction event for detection in real-time. Indirect Biosensor: ELISA REPORTER SUBSTRATE REPORTER REPORTER Substrate turnover and signal detection ANTIBODY BINDER Wash Wash ANTIBODY Incubate ANTIBODY Incubate BINDER BINDER TARGET BINDER TARGET BINDER TARGET Based upon tight binding between an antigen and antibody Labeling agents for ligands include fluorescent probes and radioisotopes Most commonly used reporter enzyme horseradish peroxidase (HRP), which upon reaction with substrate produces a bright green color Direct Biosensor: SPR Optical detection method for studying interactions between a soluble analyte and immobilized ligand Binding of the analyte molecule changes the refractive index in a way that is approximately proportional to the mass of the molecules which have entered the interface Stoichiometry of binding can be examined http://www.astbury.leeds.ac.uk/Facil/spr.htm Advantages and Disadvantages of Indirect and Direct Biosensors Indirect Advantages Amount of material required Can detect virtually any material (ELISA) Sensitivity Signal amplification Disadvantages Labeled ligands or secondary reagents required Background problems – washing is necessary Direct Advantages Binding event results in signal transduction Signal measures only the desired interaction Disadvantages Specialized machinery is often required Signals more difficult to amplify Time-consuming “Ideal” Biosensors Response is directly coupled to recognition event: Direct Signal readily detectable without the use of expensive or large instrumentation Adaptable to detect many types of analytes Outline Biosensors Definition and Introduction Direct and Indirect Polydiacetylenes Polymerization Reaction Chromic Response to Environmental Changes Harnessing Chromic Response in a Useful Biosensor Construct Variables Associated with Designing an Appropriate PDA Biosensor for a Variety of Systems Diacetylene Polymerization Topochemical polymerization reaction Reaction is very sensitive to the surrounding environment and packing of substituents Reacting carbon atoms must be less than 4 Å away from each other or polymerization is not likely to occur R1 + R2 R1 R1 + R2 R2 R2 R1 R1 R2 R2 R1 Mechanism of Polymerization Reaction R1 R1 + R2 R1 R1 hν + R2 R1 C C R2 R2 R1 C R1 C R2 C R2 R1 R1 R2 R2 R1 R2 C R2 R2 R1 R1 C R2 R2 C R2 C R2 R1 R2 R1 R2 C R1 R1 R1 PDA Response to Environmental Changes R2 R1 R1 R2 R2 R1 R1 R1 R1 R1 R2 R2 R2 R1 R1 R1 C C R2 R2 R2 R2 n R2 R1 C C R2 R1 C C R2 Blue Phase (ppm) 131.6 107.4 C C R2 n No butatrienic structure indicated in either blue or red form as indicated by Carbon >C= −C≡ R1 R1 13C NMR Red Phase (ppm) 132.0 103.6 Tanaka, H.; et. al. Macromolecules 1989, 22, 1208. Effect of Side Chain Conformation on Chromatic Response Carbon δ-CH2 α-CH2 ε-CH2 β,γ-CH2 Blue (ppm) 66.6 37.3 32.9 24.5 Red (ppm) 65.5 37.8 32.6 26.4 R1 R1 R2 Only β,γ-carbons show significant shift between 2 phases Conformational change around backbone single bonds is minimal as α-carbon chemical shifts to not change significantly R1 O O N H R2 Tanaka, H.; et. al. Macromolecules 1989, 22, 1208. Polydiacetylenes as Biosensors R1 R1 R1 R2 R2 R2 R1 R1 R2 R2 n Incorporation into vesicles Methodology of assay Physical changes in vesicles and relationship to color change Variables associated with appropriate biosensor design Position of diacetylenic functionality Incorporation of recognition element ? Two Supramolecular Approaches for Utilizing Polydiacetylenes in Sensors Immobilization of polymer as a thin film on a solid glass support HO O HO O HO O HO O HO O HO O Solution-based sensors incorporating PDA vesicles (liposomes) HO O HO O HO O O HO OH O O HO OH O O HO OH O O OH O OH O OH Advantages of Vesicles Liposomes can be made more simply and reproducibly Vesicle assays and analysis can be done in a 96-well plate format Liposomes mimic the cell membrane more closely than thin films Ability to immobilize and remain functional on a surface HO O HO O HO O HO O HO O HO O Vesicle Immobilization onto Au Films Use lipid with disulfide containing headgroup to immobilize vesicles on gold Disulfide remains oxidized, reducing vesicle aggregation Vesicles remain highly monodisperse over periods of 3 days in buffer Stanush, I.; Santos, J.; Singh A. J. Am. Chem. Soc. 2001, 123, 1008. PDA Vesicle Stability Stable at 4ºC in solution for months Can be made stable to lyophilization and resuspension Not sensitive to white light Show no evidence of fusion to form large aggregates Not destroyed osmotically by salts PDA Vesicles: Synthesis Diacetylenic monomer molecules self-assemble into an ordered array by the same driving forces which occur in the formation of biological membranes Vesicle formation is encouraged by sonication Monitor polymerization reaction by appearance of a deep blue color Design of Colorimetric Assay Analyze peptide-membrane interactions Utilize well-characterized antimicrobial peptides and related mutants to examine interactions at vesicle surface and colorimetric response (CR) Amphiphilic peptides severely disrupt membrane surface and may insert into membrane and form pores Vesicles contained 6:4 mole ratio of TRCDA and phospholipid (e.g. DMPC) OH O ( )m O O O O O P O O O- + N DMPC M = 6; N = 7 TRCDA ( )n Kolusheva, S.; Boyer, L.; Jelinek, R. Nature Biotechnology 2000, 18, 225. Kolusheva, S.; Shahal, T.; Jelinek, R. Biochemistry 2000, 39, 15851. Colorimetric Assay Vesicle solutions buffered with Tris to pH 8.5 Incubate peptide and vesicles for 30 min at 27ºC and measure CR Control: no peptide Cells containing amphiphilic peptides Kolusheva, S.; Boyer, L.; Jelinek, R. Nature Biotechnology 2000, 18, 225. Kolusheva, S.; Shahal, T.; Jelinek, R. Biochemistry 2000, 39, 15851. Colorimetric Response (CR) ABlue ABlue ABlue ARed 0 ABlue ARed f100 ABlue ABlue ARed 0 Calculation of quantitative value for extent of color transition from initial blue state to final red state A: absorbance at the “blue” (~640nm) or “red” (~500nm) Depending upon background levels and non-specific interactions, interactions can be detected with as little as 5-7% CR Kolusheva, S.; Boyer, L.; Jelinek, R. Nature Biotechnology 2000, 18, 225. Kolusheva, S.; Shahal, T.; Jelinek, R. Biochemistry 2000, 39, 15851. Non-Specific PDA-Analyte Interactions Measure CR with pure PDA vesicles to determine changes due to interactions between analyte and negatively charged PDA portion of vesicles Even at μM concentrations, melittin can be detected above background Kolusheva, S.; Boyer, L.; Jelinek, R. Nature Biotechnology 2000, 18, 225. Kolusheva, S.; Shahal, T.; Jelinek, R. Biochemistry 2000, 39, 15851. Negative Controls Expose vesicles to mismatched analyte to rule out CR resulting from non-specific interactions with the recognition element Examine response due to presence of peptides not expected to be membrane active (e.g. neuropeptides) Peptide-membrane interactions are non-specific; use to ensure CR is due only to membrane interactions and disruption and not presence of other analytes No peptide Antimicrobial Peptides Neuropeptide (no membrane interaction) Kolusheva, S.; Boyer, L.; Jelinek, R. Nature Biotechnology 2000, 18, 225. Kolusheva, S.; Shahal, T.; Jelinek, R. Biochemistry 2000, 39, 15851. Polydiacetylenes as Biosensors R1 R1 R1 R2 R2 R2 R1 R1 R2 R2 n Incorporation into vesicles Methodology of assay Physical changes in vesicles and relationship to color change Variables associated with appropriate biosensor design Position of diacetylenic functionality Incorporation of recognition element ? Mechanisms of Biochromatic Response Effective length of conjugation in the polymer shortens as a result of desired interaction resulting in a strong blue-red color transition Insertion of viral membrane or toxin hydrophobic domains into the PDA bilayer Multipoint interactions of the receptor at the PDA-vesicle surface changing packing of lipid headgroups near surface Observation of Physical Changes in Vesicles in Conjunction with CR OH O Detection of antibody-epitope recognition ( )m O O O HA peptide-epitope is presented at the N-terminus of a hydrophobic -helix designed to span lipid bilayers O O P O -O O + N DMPC m = 6; n = 7 TRCDA ( )n Kolusheva, S.; et. al. J. Am. Chem. Soc. 2001, 123, 417. Antibody-Epitope Interaction Results in a Physical Change Prior to addition of antibody Vesicles Blue Incubated with HA antibody Vesicles Red Incubated with incorrect antibody Vesicles Blue Kolusheva, S.; et. al. J. Am. Chem. Soc. 2001, 123, 417. Varied Mechanisms of Membrane Interaction Evidence of phospholipase activity : PLA2, PLC, and PLD Enzymes which hydrolyze cell membrane phospholipids Each enzyme cleaves PC in a different location, but activity of each results in a similar colorimetric response PLA2 PLC O O O O P O -O O PLD + N O Jelinek, R.; et. al. Chem. Biol. 1998, 5, 619. Okada, S.; Jelinek, R.; Charych, D. Angew. Chem. Int. Ed. 1999, 38, 655. Cleavage Products Disrupt Membrane O OH + O OH O O O P O N O - + O OH + O HO O O P - O O N + PLC (phosphodiesterase) Cleavage product is 1,2-diacylglycerol Lipid chains spread apart and expose hydrocarbon core to aqueous surface PLC PLD (phosphodiesterase) O O O O O Cleavage products leave membrane matrix Forms “pits” in membrane surface, resulting in changes in lipid packing PLA2 O PLA2 (acyl hydrolase) O P HO O + HO N + Cleavage product is phosphatidic acid (PA) PA has affinity for Ca2+ ions in buffer Interaction with cations results in vesicle condensation PLD Jelinek, R.; et. al. Chem. Biol. 1998, 5, 619. Okada, S.; Jelinek, R.; Charych, D. Angew. Chem. Int. Ed. 1999, 38, 655. Polydiacetylenes as Biosensors R1 R1 R1 R2 R2 R2 R1 R1 R2 R2 n Incorporation into vesicles Methodology of assay Physical changes in vesicles and relationship to color change Variables associated with appropriate biosensor design Position of diacetylenic functionality Incorporation of recognition element ? Location of Polymerization Group 10,12-PDAs have a much more rigid hydrophobic chain prior to the diacetylene moiety OH O ( Strong connection between conformation of alkyl chain and polymer electronic properties 5,7-PDAs are expected to be more responsive to environmental changes )m m = 0 or 6 m = 0: TCDA and DCDA m = 6: TRCDA and PCDA ( )n Thermochromism of 5,7- and 10,12-PDAs Examine thermochromism in response to incubation at 50ºC as a function of time Vesicles composed of 5,7-PDAs express an enhanced response compared to 10,12-PDAs Drawback of this enhanced response is that 5,7-PDAs are more readily affected by properties of their solution: salt content, pH, etc Okada, S.; et. al. Acc. Chem. Res. 1998, 31, 229. Location of Polymer Backbone and Effective Biochromic Response Positive response to cholera toxin with 5,7-PDA vesicles (and ganglioside receptor) No response to cholera toxin with 10,12PDA vesicle Positive response to E. coli with 2,4-PDA vesicles (and sialic acid receptor) No response to E. coli with 10,12-PDA vesicles OH O OH O OH O O OH vs. vs. ( )n ( )n ( )n ( )n Pan, J.; Charych, D. Langmuir 1997, 13, 1365. Ma, Z.; et. al. J. Am. Chem. Soc. 1998, 120, 12678. Incorporation of Recognition Element Incorporated on separate membranespanning peptide in antibody-epitope studies Synthetically attach recognition element to lipid containing diacetylene moiety HO OH HO COOH AcHN O O HO O N H O O O N H Incorporate recognition element through a lipid in the system which does not contain a diacetylene moiety, and therefore cannot be polymerized Synthetic Attachment of Recognition Element Bifunctional molecule incorporates both the recognition element (sialic acid) and the reporter diacetylene moiety Surface lectin of influenza virus (hemagglutinin) binds terminal glycosides (sialic acid residues) on cell surface glycoproteins and glycolipids HO OH HO COOH AcHN O O HO O N H O O O N H O 10,12-pentacosadiynoic acid (PCDA) HO Reichert, A.; et. al. J. Am. Chem. Soc. 1995, 117, 829. PDA Vesicle Detection of Influenza Virus HA binds cell surface sialic acid residues and initiates viral infection Detection of as little as 11 HAUs of virus particle (~11 x 107 virus particles) 90 Colorimetric Response [%] 80 70 60 50 40 30 20 10 0 0 10 20 30 40 50 60 70 Amount of Virus [HAU] Reichert, A.; et. al. J. Am. Chem. Soc. 1995, 117, 829. Incorporation of Recognition Element on a Non-Polymerizable Lipid Useful when receptor of interest is already lipid linked or when attaching receptor to diacetylenic lipid may be synthetically challenging Gangliosides are lipid molecules that reside on the surface of the cell membrane and display carbohydrate recognition groups Cholera toxin recognizes GM1 ganglioside 5,7-docosadiynoic acid (DCDA) Pan, J.; Charych, D. Langmuir 1997, 13, 1365. Detection of Cholera Toxin Detection of slightly less than 100 μg/ml cholera toxin Response is slightly sigmoidal Binding cooperativity – binding one ligand makes the vesicle more accessible for others Polymer side chain conformations – once the effective conjugated length of the vesicle is perturbed as the result of toxin binding, subsequent perterbation is more favorable Pan, J.; Charych, D. Langmuir 1997, 13, 1365. Screening a Library with PDA Vesicles NH2 Examine structure-activity relationships in a library of amphiphilic co-polypeptides Relationship between polypeptide -amino acid composition and interaction with phospholipids found in cell membranes Suggest important factors for designing new antimicrobial peptides N H O Lys N H O N H O N H Ala N H Ile O Phe N H O Leu O Val Wyrsta, M.; Cogen, A.; Deming, T. J. Am. Chem. Soc. 2001, 123, 12919. Detection of Peptide-Membrane Interactions Most membrane-active peptides are of intermediate chain length and high hydrophobic content Peptides containing -helix favoring amino acids interact with vesicles and produce a colorimetric response Peptides containing β-sheet favoring amino acids do not produce any colorimetric response Ala, Phe, and Leu: α-helix favoring Ile and Val: β-sheet favoring B) Lys/Ala peptides C) Lys/Phe peptides D) Lys/Leu peptides E) Lys/Ile peptides F) Lys/Val peptides Blue = negative Red/Orange = positive Wyrsta, M.; Cogen, A.; Deming, T. J. Am. Chem. Soc. 2001, 123, 12919. Future Directions Continue to examine the mechanism of PDA biochromic response Apply vesicle methodology in evaluation of compounds with unknown activity (e.g. potential antimicrobial peptides or enzyme inhibitors) Correlate colorimetric response with desired biological interaction Examine biochromic responses in new constructs and immobilized vesicles ? Conclusions Polydiacetylene vesicles mimic the properties of cell signaling by directly coupling a bio-recognition event to signal transduction Recognition events in a PDA vesicle result in a visible colorimetric signal which changes from blue to red PDAs are able to detect peptide-membrane interactions, antibodyepitope recognition, enzyme binding and catalysis, and virus and toxin molecule recognition If a relationship between the colorimetric response of PDAs and desired bio-recognition events can be shown, PDA vesicles could become a useful sensing technique with a wide variety of applications Acknowledgements Gellman Group Nick Fisk Terra Potocky Tim Peelen Jon Lai Marissa Rosen Erin Carlson