Download Fatty acid oxidation

Fatty acid oxidation
Fatty acid oxidation (FAO) is the primary metabolic
pathway in a variety of tissues, becoming particularly
important during periods of glucose deprivation. In
organs such as liver and skeletal muscle, FAO can provide
over 75% of cellular ATP while in cardiac tissue it can be
responsible for up to 90% of cellular energy requirements.
The primary pathway for the degradation of fatty
acids is mitochondrial fatty acid β oxidation. Long
chain fatty acids (LCFAs) are imported into the
mitochondria as acyl carnitine. Once inside, acylCoAs
are released to undergo an iterative four-step oxidation
until the entire chain is oxidized to acetylCoA, while
carnitine returns to facilitate further LCFA transport.
The Acetyl CoA produced typically enters the TCA,
although in liver it can also fuel the production of ketone
bodies, an important energy source for other tissues.
Both TCA and β oxidation contribute to the pool of reducing
equivalents (NADH and FADH2) which, in turn, drive
the activity of the ETC and subsequent ATP generation.
www.amsbio.com | [email protected]
To facilitate the convenient measurement of FAO-driven respiration, AMSBIO offers the
MitoXpress® Xtra FAO Kit (MXC-500). The kit is designed for use with the MitoXpress® Xtra HS
- Oxygen Consumption Assay (MX-200) and contains the 18C unsaturated fatty acid Oleate as
substrate, supplied as a 2:1 BSA conjugate. The kit also contains a buffer tablet and L-Carnitine
for convenient preparation of measurement media and two FAO modulators, Etomoxir and FCCP.
Etomoxir inhibits CPT1 thereby preventing Oleate import, limiting the supply of reducing equivalents to the
ETC, and, in turn, reducing oxygen consumption. The remaining ETC activity is driven by non-long chain FAO.
FCCP treatment induces maximal ETC activity by dissipating the mitochondrial membrane potential,
while the increased demand for reducing equivalents causes a concomitant increases in FAO
activity. This increase is limited where exogenous LCFA is unavailable or where import is inhibited.
Products
Description
MitoXpress® Xtra Fatty Acid Oxidation Kit
MitoXpress® Xtra HS - Oxygen Consumption Assay
MitoXpress® Xtra HS - Oxygen Consumption Assay
Pack Size
96 reactions
96 reactions
4x96 reactions
Cat No
MXC-500
MX-200
MX-200/4
Description
pH-Xtra™ - Glycolysis Assay
pH-Xtra™ - Glycolysis Assay
Pack Size
96 reactions
4x96 reactions
Cat No
PH-200
PH-200/4
MitoXpress® Intra - Oxygen Concentration Assay
96 reactions
MX-300
MitoXpress® Intra - Oxygen Concentration Assay
5x96 reactions
MX-300/5
Other Products
AMSBIO is the global source of all the products listed here. All trademarks mentioned here are the property of their respective owners.
© AMSBIO / AMS Biotechnology (Europe) Ltd Dec 2016