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Aquatic Restoration Lakes Unit 6, Module 24 November 2003 Objectives Students will be able to: differentiate between restoration, rehabilitation, and reclamation. compare and contrast functional restoration and structural restoration. define lake restoration and describe its major goals. describe current statistics regarding lake stressors. identify special challenges encountered in lake restoration. define and identify ecoregions in Minnesota. indicate preventative steps used to control sources of water pollution. describe the impact of phosphorus in a lake environment. discuss measures used to limit the impacts of phosphorus on lakes. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s2 Objectives cont. assess the effectiveness of lake restoration through analysis of site examples. identify methods used to control levels of algal growth in lakes. discuss the effectiveness and concerns of lake dredging. describe the goals and principles of biomanipulation used in lake restoration. describe four types of zooplankton and explain their importance. discuss the effectiveness and concerns of using algicides. identify physical methods used for controlling weed growth in lakes. discuss chemical and biological methods for controlling weed growth in lakes. relate the principles of lakescaping. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s3 Overview Introduction 1. Lake Restoration 2. Stream Restoration 3. Wetland Restoration Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s4 Restoration Defined “Return an ecosystem to a close approximation of its condition prior to a disturbance” (Berger 1990) “Act of restoring to the original state or a healthy or vigorous state” (Bradshaw 1996) Historic conditions previously existing on the site will be re-established, including the entire function, structure, and genetic composition Return of fundamental processes by which ecosystems (biological and non-biological elements) work Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s5 Restoration, rehabilitation or reclamation? Restoration to historic conditions as a goal is often either impossible due to land use changes in the watershed or too expensive Rehabilitation: returns certain functions and structures of the natural ecosystem at a previous state, but not the original condition Reclamation: provides services by returning certain functions and structures bringing the ecosystem to a useful state, but something other than the original condition Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s6 Function or structure? What are you trying to restore? Examples of Functions Surface and groundwater storage, recharge, supply Floodwater and sediment retention Transport of organisms, nutrients, sediments Nutrient cycling Biomass production, food web support, species maintenance Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s7 Function or structure? What are you trying to restore? Examples of Structure Soil condition Geological condition Hydrology Water quality Topography Morphology Flora and fauna Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s8 Ecosystem Function Reclamation Original Ecosystem Restoration Rehabilitation Degraded Ecosystem (Modified from Bradshaw 1996) Developed by: Axler and Reed Ecosystem Structure Updated: 11/26/03 Lakes U6-m24a-s9 Setting Project Goals “No one paradigm or context for setting restoration goals” (Ehrenfeld 2000) In the past, goals usually focused on: • Single species • Drawback: changes might be counterproductive for other species • Ecosystem functions • Drawback: managers might either have specific techniques in mind or only focus on obvious degradations • Services • Drawback: could negatively impact functions or species Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s10 Basic Steps for Setting Goals 1. Identify stressors causing degradation Consider what scale should be used 2. Determine goals What are the ecological and socio-economic limitations? 3. Develop techniques to reverse degradation What are the key processes involved with each stressor? 4. Adaptive management What measurements should be taken during the project to adapt restoration techniques? Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s11 Lakes vs Rivers vs Wetlands Lakes Major impacts affect water quality Most restoration methods focus on physical, chemical and biological manipulation of the water and sediments Rivers Major impacts effect channel shape and bed sediments Most restoration methods focus on manipulation of the physical environment (channel) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s12 Lakes vs. Rivers vs. Wetlands Wetlands Major impacts effect wetland hydrology Restoration methods focus on restoring original hydrology Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s13 Overview Introduction Lake Restoration Stream Restoration Wetland Restoration Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s14 Lake Restoration – a definition “Shift lake to a valuable, resilient system in which ecosystem processes (structures and functions) are maintained for a given state when subjected to disturbance” (Ludwig) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s15 Lake Restoration – another definition “Emulate a natural, self-regulating system that is integrated ecologically with the landscape in which it occurs” (NRC 1992) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s16 Lake Restoration – and yet another “To return the lake to a long-term, steady state condition similar to its pre-disturbance condition and in accord with reasonably attainable conditions, as dictated by the characteristics of the ecological region” (Cooke et al. 1993) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s17 Restoration vs Management vs Protection Restoration Return to a previously acceptable condition (or as close as possible Management Improvement to enhance the stated beneficial uses such as swimming, fishing, water supply, wildlife habitat, etc Protection Prevention of adverse impacts Continued management and possible reapplication of restoration techniques Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s18 State of the Lakes Approximately 40.6 million acres of lakes in U.S. (navigable waters including reservoirs) Great Lakes Status State of the Lakes Conference is a biennial conference that presents estimates of the environmental health of the Great Lakes http://www.epa.gov/glnpo/solec/ Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s19 State of the Lakes Of the 17.3 million acres assessed in 2000, 45% impaired for one or more intended uses Aquatic life support, fish consumption, primary and secondary contact, drinking water supply, agriculture (irrigation and stock watering), industrial Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s20 EPA, 2000 Lake Stressors Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s21 Lake stressors STRESSOR ACRES Unknown 3,663,901 21.1 Agriculture 3,158,393 18.2 Hydro-modification 1,413,624 8.2 Urban runoff 1,369,327 7.9 Natural sources 1,066,925 6.2 Atmospheric deposition 983,936 5.7 Municipal point sources 943,715 5.4 Land disposal 856,586 4.9 Construction 691,100 4.0 Grazing 615,125 3.5 Habitat Modification 540,207 3.1 Developed by: Axler and Reed PERCENT Updated: 11/26/03 Lakes U6-m24a-s22 General classes of human activities and the stressors associated with them glei.nrri.umn.edu Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s23 How human activities link to specific stressors glei.nrri.umn.edu Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s24 Resultant problems Nuisance algae Nuisance vascular plants Acidification Anoxia and related issues Toxic substances Pathogens Non-algal color and turbidity Sediment buildup Undesirable fisheries In other words …. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s25 Special challenges in lake restoration 1. Reservoirs Different hydrology and morphometry Large inflow tributary Generally large watershed, many shallow bays Many competing uses results in diverse restoration goals 2. Ecoregion concept Not all lakes are created equally Some lakes will always be more productive than others Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s26 Special challenges in lake restoration Reservoirs Different hydrology and morphometry Large inflow tributary from far upstream Generally large watershed, many shallow bays Many competing uses Results in diverse restoration goals; more recently this includes actual dam removal Ecoregion concept Not all lakes are created equally Some will always be more productive than others Productive lakes are not necessarily “bad”; they may be naturally more eutrophic and support better fisheries Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s27 Increased algae isn’t always bad… Increased algal growth leads to decreased water clarity (secchi depth) BUT More food at base of food web leads to increased fish yield BUT Not always the fish you want Schematic from NALMS. 1990. The lake and reservoir restoration guidance manual. 2nd edition. North American Lake Management Society and USEPA Office of Water, Washington, D.C. EPA-440/4-90-006 August 1990. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s28 What are Ecoregions ? Areas with similar: Climate Landuse Soils Topography “Potential” natural vegetation Minnesota has seven major ecoregions Four ecoregions contain most of the lakes Water quality varies greatly from south to north Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s29 Minnesota’s Ecoregions Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s30 1.0-3.3 ft 8-15 ft 5-11 ft 1.6-3.3 ft Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s31 Source control – Prevention - 1 Prevention: The First Step • Ensure that point sources are controlled by best available technologies (BATs) • Regulated by the National Pollutant Discharge Elimination System – NPDES Permits • Improve on-site wastewater collection and treatment systems • Leaky systems add nutrients, organic matter and pathogens to lakes Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s32 Fix problem septics systems and leaky sewer lines (infiltration & exfiltration) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s33 Onsite wastewater treatment - Minnesota Issues • 30% of Minnesotans use onsite (decentralized) septic treatment systems; ~25% nationally • ~50% failing or improperly designed (>250,000 residences in Minnesota) • ‘Limited’ soils, wet spring, high water table, frozen soils, small lots, sensitive water supplies • Immediate public health hazards • Longer-term nutrient issue (eutrophication) • Development pressures on lakes increasing • Conventional systems may be ineffective with few or no available alternatives; unique cold climate problems Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s34 Source control – Prevention - 2 Control urban and agricultural runoff Control erosion stabilize the shoreline; protect the riparian zone Maintain vigorously growing filter zone of grass, shrubs and trees next to water surface Stabilize eroding bluffs Route drainage away Establish vegetation Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s35 Source Control - 3 Capture pollutants (retention and detention basins, constructed wetlands) Control fertilizer usage and grass clipping runoff “Manage” hydrology to reduce peak flows Reduce impervious surface Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s36 Lake Restoration General goals: Control excessive plant growth Control nuisance algae Improve clarity (algae and/or suspended sediment) Improve habitat for desirable species Remove or control nuisance organisms Alleviate oxygen depletion Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s37 Lake restoration methods Methods • Physical Controls • Mechanical manipulation of water and/or sediment • Chemical Controls • Precipitants and herbicide addition to control “limiting nutrients (P), suspended sediment, acidification or organisms (algae, invasive “weeds”, “trash” fish) • Biological Controls • Introduction or removal of organisms for controlling algal blooms, invasive weeds, or other aquatic nuisance species (exotic and invasive) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s38 Phosphorus review The next eight slides are duplicates from Unit 1 Modules 2+3 (Lake Ecology) They review the basic characteristics of phosphorus and its distribution in lakes before the next set of slides that focus on in-lake P control They discuss the link between anoxia, sediment P- release and algal blooms using examples from WOW lakes in the urban Twin City region of Minnesota. Lake Onondaga, NY and Shagawa Lake, MN are other good examples Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s39 Where does phosphorus come from? Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s40 Phosphorus – external sources Watershed discharge from tributaries (Non-point) Strongly tied to erosion (land use management) Stormwater (urban and rural) runoff Agricultural & feedlot runoff On-site domestic sewage (failing septic systems) Sanitary sewer exfiltration (leaky sewer lines) Atmospheric deposition in more pristine areas from dust, soil particles and waterfowl (Non-point) Municipal wastewater (treated), combined sewer overflows (CSOs) and sanitary sewer overflows (SSOs); industrial discharges – mostly Point Source Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s41 Phosphorus – internal sources Mixing from anoxic bottom waters with high PO4-3 tied to Fe redox reactions at “high” O2 (just > ~1 mg/L), Fe forms insoluble ferric salts (Fe+3 ) that precipitate, settle to the bottom, and adsorb PO4-3. This prevents much from diffusing up into the hypolimnion under anoxic, reducing conditions (O2 <1 mg/L), the ferric ion is reduced to the soluble ferrous ion (Fe+2 ) which dissolves – allowing sediment phosphate to diffuse up into the water wind mixing from storms and during fall destratification can reinject this high-P water to the surface causing algal blooms • Where lakes have been historically polluted by high Pinputs, this reservoir of P can exceed annual watershed inputs (see Halsteds Bay & Shagawa data) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s42 Phosphorus – lake budget Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s43 Halsteds Bay late summer mixing events • Run the color mapper from April 1999 through 2002 focusing on storm events in mid August 1999 and 2000 • START with MAP = TEMP and plot = DO to show variable stratification • Then switch to MAP = DO and PLOT = TEMP to show anoxic events and discuss the release of P from sediments that swamps annual P-inflow from watershed Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s44 Medicine Lake– Algal blooms & mixing events - 1 Background: • Medicine Lake is extremely productive because of historically high nutrient enrichment from its watershed (go to http://lakeaccess.org/lakedata/lawnfertilizer/mainlawn.htm) • Major blooms of algae can be detected in the RUSS data set as: • supersaturated O2 (why ?) • increased pH (why ?) • increased chlorophyll-a or turbidity (why ?) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s45 Medicine Lake – Algal blooms & mixing events-2 Thursday Color = O2 Saturday Line = pH Sunday Friday-midnight STRATIFY RE- STRATIFY MIX Developed by: Axler and Reed MIX Updated: 11/26/03 Lakes U6-m24a-s46 Halsteds Bay – Algal blooms & mixing events- 3 Why did the phosphorus in the bottom water drop so dramatically in August 1999 in Halsteds Bay ? P levels drop Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s47 Halsteds Bay – Algal blooms & mixing events- 4 First, focus on the ice-free season water quality • relatively high epilimnion (surface)TP ~ 75-150 ugP/L • chlor-a (algae ) builds up steadily to levels > 50 ug/L Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s48 Halsteds Bay – Algal blooms & mixing events- 5 See how secchi drops as chlorophyll increases ? Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s49 Halsteds Bay – Algal blooms & mixing events- 6 Now see how much TP is in the hypolimnion Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s50 Halsteds Bay – Algal blooms & mixing events- 7 Summary slide without animation Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s51 Medicine Lake - Storm mixing events •This sequence runs from 1-5 from Aug 29-30, 1999 C Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s52 Phosphorus inactivation Goals: • • Control algal blooms and noxious algae Increase water transparency Mechanisms: • • • Decrease water column Pconcentration Decrease internal P-loading from sediments Decrease suspended particle concentration (due to algae and/or inorganic silt and clay) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s53 Phosphorus inactivation assumptions Assumptions: P concentration limits algal growth Reducing ortho-P and particulate- P will result in significantly reduced algal growth The water quality benefits outweigh the cost of the treatment The beneficial effects of the treatment will persist for some number of years this includes public perceptions as well as actual water quality improvements and economic costs Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s54 Phosphorus precipitation and inactivation Summary of method: • • • • Aluminum, iron or calcium salts are added Floc forms an adsorptive lattice that binds orthophosphate and small suspended particles (algae, silts and clays) Floc coagulates and settles to the bottom Overall effect: • Improved clarity • Reduced water column P-concentration • Reduced rate of P-release from the sediments Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s55 Alum treatment - reactions Chemical Reactions: • Aluminum salts such as alum (Al2(SO4)3. 14H2O)) and sodium aluminate (Na2Al2O4) form an insoluble, hydroxide floc that adsorbs strongly charged anions such as phosphate (-3) particularly well: Al+3 (from dissolved salt) + H2O Al (OH)3 + H+ • Some orthophosphate binds directly to the salts to form insoluble aluminum phosphate that precipitates out of solution: Al+3 + HnPO4-3 AIPO4 (solid precipitate) + nH+ where n = 1 or 2 depending on pH Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s56 Alum and Lime Lab - dosage rates • Jar tests to estimate dosage • Adapted from drinking water treatment methods for particle removal • Boat application • Surface propwash or deep injection via hose • Barge for surface or deep delivery Developed by: Axler and Reed www.teemarkcorp.com/sweetwater/ Updated: 11/26/03 Lakes U6-m24a-s57 Alum application - notes • Dosage: typically 50-500 gallons/acre depending on • Ratio of internal to external P-loading • Sediment-P levels • Morphometry (volume, thermocline, sediment area, etc) • Liquid alum contains 4.4% Al+3 • Liquid sodium aluminate contains 10.4% Al+3 • Buffering: 1 part sodium aluminate : 2 parts alum (vol) • Project managers assume <50% efficiency in terms of the expected removal of P based on theoretical removal and on how evenly the floc blankets the lake bottom • Effectiveness 7~10 years typically Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s58 Alum treatment - summary Effectiveness: • • • • Widely used, high success Rapid, fairly long-term (<10-15 years) Needs to be buffered (pH 6-8) to reduce toxicity Not effective if external loading and sedimentation are not controlled • Works well for lakes with extensive littoral and wetland areas • Effective in spite of anoxia which decreases the effectiveness of iron treatment Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s59 Other coagulants - Iron Iron Usually added as ferric chloride (FeCl3) at 3-5 mg Fe/L Forms an insoluble ferric hydroxide complex [Fe(OH)3.nH2O] that precipitates as a rusty floc Adsorbs phosphate and also reacts with phosphate to form Fe(PO4) that is insoluble However - the floc dissolves and P is released under anoxic, reducing conditions where Fe+3 (ferric ion) is reduced to Fe+2 (ferrous ion) which is soluble Very effective in water treatment plants where oxygenated conditions prevail Also shown to be effective in shallow, eutrophic lakes in the Netherlands despite occasional anoxia Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s60 Other coagulants - Calcium Usually added as either lime [Ca(OH2)], powdered limestone [CaCO3], or a mixture At high pH and high Ca+2, the lime dissociates to form small particles of calcium carbonate that precipitates Ca(OH)2 + CO2 CaCO3 + H2O (lime reaction) Large surface area for adsorbing PO4 and particles Demonstrated effectiveness in small agriculturally impacted lakes in Alberta, Canada (called dugouts) Naturally hard water Drinking water problems associated with blooms of blue-green algae (cyanobacteria) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s61 Calcium – acid rain mitigation Calcium minerals have also been added to lakes, streams and even watersheds to buffer the effects of acid rain www.epa.gov/airmarkets/acidrain/index.html “Liming” neutralizes the acid which improves the pH and causes aluminum to convert to an inert, nontoxic form. It is not a “cure” for the problem. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s62 Calcium – Thrush Lake, MN liming Acid deposition sensitive streams, lakes and watersheds were limed as tests of strategies for mitigating potential acidification impacts May, 1988 • +4.5 tonnes of powdered CaCO3 doubled alkalinity • Too small a dose to affect TP Acid Precipitation Mitigation (APMP) Program funded by the US Fish and Wildlife Service (late 1980’s) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s63 Coagulants - safety issues Handling large quantities of chemicals Liquid alum and ferric chloride are acidic and corrosive. Buffered alum, either with sodium aluminate or calcium carbonate is more convenient Quick-lime, CaO is sometimes used but it is very caustic and dangerous to handle Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s64 Phosphorus precipitation examples (alum) Example: West Twin Lake, Ohio • Dimictic • Area: 30 ha • Max. depth: 11 m Impacts: • Frequent algal blooms Goal: • Lower P in epilimnion by reducing hypolimnetic P Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s65 West - East Twin Lake experimental design Treatment methods (dosed in summer 1975) • Divert wastewater inputs (1971-1972) at both sites to minimize external P-loading • Treat West Twin Lake hypolimnion with alum (26 mg Al/l in the form of liquid aluminum sulfate) • Use East Twin Lake as a “control” Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s66 West - East Twin Lake results Rates of release in bottom meter of water “Control” West Twin Lake (mgP/m2.d) East Twin Lake (mgP/m2.d) 1973 4.24 2.83 1974 1.51 2.80 1975 2.68 2.67 1976 0.37 1.76 1978 0.67 3.34 1980 0.00 1.06 1989 2.02 4.05 “Alum” Developed by: Axler and Reed • P-release decreased for about 7 yrs • Possibly until 1989 (~16 yrs) Updated: 11/26/03 Lakes U6-m24a-s67 West - East Twin Lake - more results Evaluation: • Initial increase of P in the treatment lake was most likely from an untreated littoral-wetland area • Internal loading was reduced for > 6 years • Even with the so-called “control” lake, the success of the combination of techniques used (diversion and alum treatment) was not clear for the long-term Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s68 Mirror Lake, WI - alum treatment Background: Hypereutrophic urban lake • Area: 13 ac (5.3 ha) Zmax: 13 m • Major cause: stormwater-P (>50% of P-inputs) Developed by: Axler and Reed Mirror Lake, Waupaca County, WI Updated: 11/26/03 Lakes U6-m24a-s69 Mirror Lake alum treatment - results VOLUME WEIGHTED MEAN PHOSPHORUS Graph from Holdren, C., W. Jones, and J. Taggart. 2001 Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s70 Mirror Lake - results Restoration strategy: • 1976- divert storm sewers;decrease P-loading >50% • 1978- alum application to bind sediment-P and fall re-aeration to prevent winter-kill of fish from low O2 Interval TP-water (ug/L) P-release (mgP/m2/d) Re-alum 1978 >80 1.3 Alum 1978-1981 ~ 20 0.07 Post-alum 1990 Developed by: Axler and Reed ~ 20-32 0.20 Updated: 11/26/03 Lakes U6-m24a-s71 Dilution flushing Goal: Control Nuisance Algae Method: Dilute the concentration of nutrients within the lake by adding nutrientpoor water, reducing growth Flush algae out of the lake faster than they can reproduce Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s72 Dilution flushing - limitations Limitations: Finding an economically affordable source of low nutrient water for flushing Potential impacts to downstream structures and ecosystems due to increased discharge Usually feasible only for small systems Commonly used for small urban park and golf course ponds Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s73 Aeration & Circulation– Goals & Methods Goals: Improve habitat for fish and invertebrates Disrupt algal buildup and growth Drinking water improvement (taste/odor/staining) Methods: Add air or O2 to epilimnion, hypolimnion or under ice Sustain fish during O2 stress Inhibit P release from sediments Precipitate P from anoxic hypolimnetic water Oxidize organic matter and toxic H2S and NH3 Oxygenation may use gas bubbling or liquid (LOX) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s74 Aeration and Circulation – systems Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s75 Aeration and Circulation – methods (cont) Methods: Circulate water horizontally to alleviate low oxygen or disperse localized algal scums (bays and coves) Mix water vertically for aeration and/or to light limit algae where nutrient control is not feasible Mix high CO2 water into epilimnion to retard scum- forming species of blue-green algae (cyanobacteria) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s76 Aeration and Circulation - problems “Side” effects: May exacerbate algal and turbidity problems by re- suspending: Surface sediments Sediment- P Hypolimnetic- P, NH3, and H2S Anoxic water View August 1999 Halsteds Bay, MN sequence to see how surface water quality can be affected Resting blue-green algae “spores” Many species of noxious bloom formers form resting stages that settle to the bottom and are re-introduced during mixing events that bring them and nutrients into the euphotic zone Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s77 Hypolimnetic Aeration Goals: Introduce O2 into hypolimnion for: Habitat improvement for fish Eliminate taste and odor problems in drinking water sources (especially reservoirs) Precipitate Fe and Mn (to reduce staining) Inhibit sediment P-release Developed by: Axler and Reed anoxia Updated: 11/26/03 Lakes U6-m24a-s78 Hypolimnetic Aeration Either avoid destratification •0 •Eutrophic To maintain thermal regime for a “2-story” fishery To avoid introducing excessive nutrients and toxic H2S and NH3 into surface waters Or destratify if desirable to overall goals Developed by: Axler and Reed T NO3 anoxia NH4 PO4 Updated: 11/26/03 Lakes U6-m24a-s79 Hypolimnetic aeration with destratification Holdren, C., W. Jones, and J. Taggart. 2001. Managing Lakes and Reservoirs. NALMS. EPA 841-B-01-006. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s80 Case study: Camanche Reservoir, CA “Speece” cones are one of many commercial systems available for aerating reservoir hypolimnia and reservoir tailwaters Comanche Reservoir Issues • • • • Drinking water and irrigation Salmon and trout fishery Hydropower and flood control Downstream discharge problems associated with low hypolimnetic O2 and high H2S Developed by: Axler and Reed www.eco2tech.com Liverpool Water Updated: 11/26/03 Lakes Witch Barge U6-m24a-s81 Hypolimnetic withdrawal- goals Goals: • Remove nutrient-rich water from hypolimnion • Reduce overall nutrient levels to control algae • Improve O2 levels in hypolimnion to provide fish habitat, further reduce P-loading, and reduce sediment of toxic metals, ammonia, and H2S Method • Discharge the water in the bottom layer of a lake by siphoning, pumping, or selective release (dam) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s82 Hypolimnetic withdrawal- summary Summary: • • Relatively inexpensive Potential for long-term effectiveness by increasing O2 and reducing internal P-loading • May need for > 3-5 years to measure success • Removing too much water may destratify lake • May adversely impact stream water quality! • Creates downstream impacts due to low O2, H2S, high nutrients, thermal changes • May entrain and harm sensitive organisms Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s83 Sediment oxidation - goals Goals: Promote denitrification to reduce sediment BOD and ultimately P-release Depletes organic matter in sediments reducing internal phosphorus loading Improves P-binding with iron hydroxide complexes Prevents sulfate reduction (eliminates H2S) Oxidize the top 10-20 cm of lake sediments NO3- N2 (gas) = denitrification (anoxic) heterotrophic ("burn" organic matter using NO3-, not O2) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s84 Sediment oxidation - procedure Procedure: • Ca(NO3)2 (calcium nitrate) solution injected into the sediment to stimulate denitrification • FeCl3 (ferric chloride) may be added to precipitate H2S as FeS (ferrous sulfide) in anoxic zones and to produce Fe(OH)3 (ferric hydroxide) in oxic zones to adsorb phosphorus • Ca(OH)2 (lime) may also be added to raise pH to an optimal level for denitrification Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s85 Sediment oxidation - summary Summary: • Potential to reduce phosphorus release from sediments by 50-80% (but few case studies) • Expected to be less effective if internal P-loading is low relative to external (from the watershed) • May require iron additions to enhance P-binding • May decrease sediment oxygen demand (SOD) • Nitrate addition may exacerbate eutrophication • Relatively few studies so there have been few comprehensive evaluations of the method Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s86 Dredging: Lake sediment removal Goals: • Control excessive macrophyte growth • Remove excess nutrient-rich muck • Deepen and increase lake volume • Remove resting cysts of noxious algae • Remove contaminants Developed by: Axler and Reed In some cases it may be necessary to remove sediment rather than treat it Updated: 11/26/03 Lakes U6-m24a-s87 Dredge methods Dry – drain most of lake to be able to use conventional heavy construction equipment Wet – partial drawdown with excavation done from barges or shoreland cranes Hydraulic dredging – usually suction from a cutter head mounted on a barge. Silt curtain may be used to contain the transport of turbid water from site Usually only 10-20% solids in the pumped slurry Pneumatic dredging – air pressure to force sediment out of the lake 50-70% solids in the effluent stream Less impact from off-site transport of TSS Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s88 Dredging effectiveness and concerns Most effective if source of sediment controlled Better success with lakes that have: Smaller watersheds Lower sedimentation rates Shallow depths Long residence times Organically rich sediments May cause short-term algal blooms and clarity loss May resuspend and mobilize nutrients and toxic substances Sediment and process water disposal must be considered Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s89 Dredging schematics Clamshell dredge scoops sediments into scows that are towed to a designated disposal area and dumped Hydraulic cutterhead suction- transfers sediment slurry via connecting pipeline to settling basin on shore or adjacent basin bottom Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s90 Biomanipulation - goals Goals: • Control algal abundance where nutrient regulation is not feasible • Increase herbivore density to lower algal biomass • Manipulate food web to reduce #s of planktivorous fish and enhance the biomass of large bodied cladoceran zooplankton (e.g. Daphnia sp.) • Limit abundance of fish that disturb sediments and enhance internal P-loading and O2 depletion Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s91 Biomanipulation – principles Principles: • Noxious algal blooms in some lakes, usually urban, are impossible to control by decreasing nutrients – at least without enormous cost and decades to recover • Managing algae by enhancing the zooplankton that eat them sidesteps the more difficult nutrient issue • Cladoceran zooplankton reproduce faster and are better grazers than the smaller copepods and rotifers • Cladocerans are more vulnerable to fish predation and so planktivorous fish must be regulated or removed Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s92 Biomanipulation – zooplankton review The next few slides are taken from Unit 1 Module 2+3: Lake Ecology and summarize the characteristics of the major groups of zooplankton Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s93 Zooplankton – major groups Major groups Crustaceans Cladocerans Copepods Rotifers Ciliated protozoans many flagellated forms are photosynthetic phytoplankton Insects Migrating benthos (Mysids, Neomysids, Diaporeia…) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s94 Zooplankton – key features - cladocerans Cladocerans (e.g. water fleas) Size: 100 –300 microns Migration – can be 10’s of meters daily Slow moving (relative to copepods and hungry fish) Selective feeders (edible vs inedible algae) Parthenogenic – “r-selection”, rapid reproduction Very effective at clearing the water column Daphnia Bosmina Developed by: Axler and Reed Chydorus Holopedium Updated: 11/26/03 Lakes U6-m24a-s95 Zooplankton – key features - copepods Calanoids, Cyclopoids and Harpacticoids: Size: wide range overlaping cladocerans Cyclopoids often predatory Faster moving – less affected by fish predation Selective feeders (edible vs inedible algae) Many lifestages and slower growing – “k-selection” Distributed more evenly over day, seasons, depth Calanoid Developed by: Axler and Reed cyclopoid harpacticoid Updated: 11/26/03 Lakes U6-m24a-s96 Zooplankton - rotifers Size: small <50 microns Migration – can be 10’s of meters daily Slow moving but small size offers some protection from adult planktivorous fish Less selective feeders (algae, bacteria, protozoans, detritus, ???; not well understood) Parthenogenic – “rselection”, rapid reproduction Developed by: Axler and Reed Keratella Polyarthra Kellicottia Updated: 11/26/03 Lakes U6-m24a-s97 Other regulators of lake productivity - Grazing Top-down Model Bottom-up Model High rates of nutrient driven algal • Nutrient inputs drive algal growth growth are decreased by intense zooplankton grazing pressure (usually cladoceran Daphnids) Fishless lakes with low invertebrate predation on grazers Lakes where planktivorous fish are regulated by predatory fish (game fish) –usually managed In these cases, algae are not nutrient limited management tool = biomanipulation Developed by: Axler and Reed • Classic Pyramid N+P Updated: 11/26/03 Lakes U6-m24a-s98 Potential Top-down effects on food chains Low Predators HIGH Predators HIGH Planktivores Low Planktivores Low Zoops HIGH Algae = Smaller Zoops less grazing Low Secchi O2 stress high pH ?? Developed by: Axler and Reed Larger Zoops more grazing HIGH = Zoops Higher Secchi less O2 stress lower pH ?? Updated: 11/26/03 Lakes Low Algae U6-m24a-s99 Biomanipulation – fish management Methods • Fish control • Intensive netting • Rotenone (poison) • Stock increased #’s of piscivorous fish • Selective catch or catch restrictions • Control conditions for fish and zoop growth and survival Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s100 Biomanipulation- Summary Summary: • Considered experimental • Requires complex knowledge of food web processes (shallow lakes are particularly poorly understood) • Herbivores may not consume certain blue-greens • May be more successful in lakes without large-bodied zooplankton • May require external loading to also be controlled • Currently considered only a management tool- not a restoration technique Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s101 Lake Mendota Biomanipulation Project Lake Mendota –large, urban, limnologically “famous” lake in Madison, WI • Eutrophic with blooms of bluegreen algae • Sewage effluents diverted out of basin entirely by 1971 • Continued nonpoint pollution from agricultural and urban runoff • 1987: attempt to control blooms by a massive stocking of walleye to reduce planktivorous fish Developed by: Axler and Reed 1988 – hot summer causes summerkill of Ciscos, the major planktivore zoops increase and algae decrease for few years Ciscos recover, anglers hammer the walleye, zoops decrease and algae are back Updated: 11/26/03 Lakes U6-m24a-s102 Algicide application – copper sulfate Goal: Reduce or eliminate algae CuSO4 application (“bluestone”) = most common Can be used in potable waters Inhibits photosynthesis and N2-fixation Often chelated to maintain solubility in alkaline or hardwater, and in colored (I.e. high humic) water Widely used for periphyton control Dosage 1-2 ppm in hard water and 0.3 –0.5 in soft water Usually surface applied to treat only the upper few meters or small coves and bays or shoreline Application: broadcast granules, spray slurry, tow mesh bags filled with granules or powder behind boat Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s103 Algicides – copper sulfate notes Heavily used to remove taste and odor due to the periphytic blue-green Oscillatoria in many drinking water reservoirs Concerns about accumulation of copper in sediments and impacts on benthos Toxic to zooplankton and young fish at pre-dilution treatment concentrations Not equally toxic to all species of algae, in particular some of the obnoxious target species are less sensitive Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s104 Algicides – more about copper sulfate Will not prevent blue-green cyanobacterial “toxins” – may actually release them during treatment May deplete O2 by creating sudden influx of detritus Not a permanent solution! May need multiple applications throughout the year and from year to year Although its use is generally not favored it has not proven to be a major problem in most cases Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s105 Algicides – organic herbicides Few non-copper based algicides Endothallic acid in its Hydrothol formulation Can be effective, especially for blue-greens But is very toxic to zooplankton and is generally used only for spot application Water use restricted for several days post-treatment Biodegrades rapidly and is a hydrocarbon that does not persist and does not bioaccumulate Diquat Contact herbicide; sometimes applied with copper Usually spot application for periphyton Toxic to zooplankton but less so to fish Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s106 Aquatic herbicides – commonly used • Developed by: Axler and Reed There are many formulations, some marketed under several different names. Check with state and federal regulatory agencies for specific information Updated: 11/26/03 Lakes U6-m24a-s107 Algicides – barley straw Mode of action: Not well understood but presumed to be phenolic compounds that are by-products of decomposition Decaying straw does not kill the algae already present, but it prevents new algae from forming Barley straw is not considered detrimental to fish health or production The anti-algal activity is only produced when the straw is rotting in a well oxygenated environment. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s108 Algicides – barley straw application Typical dose: 100-250 kg/ha (~90-220 lbs/acre) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s109 Shading – dyes Goal: Attenuate light to limit algal growth Dyes are non-toxic but often regulated as algicides Usually bluish gray How much tidy bowl is needed to keep Tahoe blue ? Potential to affect stratification, O2 , higher plants and food webs Relatively expensive per volume Surface covers used for some drinking water reservoirs Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s110 Aquatic plant management – overview Nuisance growths of native and exotic macrophytes (“weeds”) are often considered to be a major problem and a focus for restoration They may be a problem because excessive growth interferes with recreation, aesthetic values and even habitat; they may also degrade water quality by mobilizing sediment nutrients and by accelerating O2 depletion during senescence (annual die-off) Because they are extremely important components of aquatic ecosystems, initial efforts should focus on defining the perceived problem, identifying the potential negative impacts of “weed” removal and then determining the “best” control measures Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s111 Plants – macrophytes – key features Curlyleafed Pondweed • Can be excessive from nutrient enrichment – especially by exotic or invasive species at disturbed sites • Ecological importance: • structural habitat & spawning site • food (invertebrates, fish, wildlife) • stabilize shoreline and sediments • nutrient cycling (sed- N&P) • may light-limit phytoplankton in productive systems • Difficult to re-establish Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s112 Watertransparency transparency –– clear state Water clearvsvsturbid turbid state • Are they really a problem ? • If not, leave them alone because removing them may create worse problems that are difficult and costly to reverse Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s113 Water clearvs vsturbid turbidstate state Watertransparency transparency –– clear -2-2 Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s114 Shallow lakes vs deeper lakes - “switches” Usually more productive – higher Aw:Ao ratio Plants vs algae Natural predominance of macrophytes over algae. Human impacts can switch them from clearwaterplants to turbid water-algae state maintained by Poor fish management (carp, exotics, …) Inadequate shoreline protection of emergent veg Boat damage Pesticide and nutrient runoff (fish, grazers, plants) Susceptible to very obnoxious algal blooms Difficult to reestablish clearwater-macrophyte state Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s115 Aquatic vascular plants- key features Reproduce sexually by flowers and seeds and/or asexually from stem fragments and shoots from roots Need light therefore highly turbid lakes won’t have dense beds of submergent plants Expansive shallow areas most conducive to extensive beds Soft sediments and high energy shorelines can limit rooting Mineral nutrition mostly via roots and so they are difficult to control by water column nutrient reduction Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s116 Weed control – physical methods 1. Benthic barriers Goal: Smother and light-limit plants; seal nutrients Natural – adding sand, clay, gravel Artificial – plastic sheets, geotextiles Major issues: Regulatory permits Expense - usually small beaches or dock areas Porous versus non-porous synthetic coverings Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s117 Weed control – physical methods 2. Dredging Goal: Remove vegetation, seeds, substrate, nutrients Dry – drain or drawdown water level Wet – draglines, backhoes, scoops, etc Hydraulic suction or cutterhead Major issues: Regulatory permits Turbidity and nutrient release May cause algal blooms Extreme habitat disturbances In-situ and off-site containment needed Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s118 Weed control – physical methods 3. Mechanical removal Goal: Remove vegetation (shoots and/or roots) Hand pulling (see Lake Smarts reference) Cutting w/o collection Harvesting w/collection Rotatilling, hydroraking, … Major issues: Regulatory permits Fragmentation may spread infestation and decrease O2 Can be selective (or not) Can increase turbidity, algae and disrupt habitats Can increase chances for invasive and exotic sp Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s119 Weed control – physical methods 4. Water level control – Drawdown Goal: Destroying vegetation by drying, freezing or mechanical removal Mostly used for modifying fish and waterfowl habitat May be intermittent or prolonged Allows for shoreline manipulation and modification Major issues: Regulatory permits Requires proper “plumbing” and disposal area Shoreline erosion. habitat and aesthetics loss Loss of water supply during drawdown May promote undesirable growth of some species Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s120 Weed control – physical methods 5. Dyes and surface covers Goal: Light limit vegetation Dyes increase extinction coefficient for light Covers may be on bottom or at surface Major issues: Regulatory permits – dye treated as an herbicide Dyes may flush out – expensive in larger system (useful in park and ornamental ponds) Dyes – may affect aesthetics Covers – interfere with recreation Covers – can affect O2 exchange with atmosphere Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s121 Weed control – chemical methods 6. Herbicides (see also algicide slides) Goal: Destroying vegetation Mostly used for fish and waterfowl habitat May be intermittent or prolonged Allows for shoreline manipulation and modification Major issues: Regulatory permits; some have swimming, drinking, fishing, irrigation, and water use restrictions (check label and general permit) Non selective removal may make matters worse Toxicity to non-target invertebrates and fish Habitat and aesthetics loss Dissolved O2 depletion from rotting vegetation Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s122 Herbicides – the major chemicals used Copper sulfate – more often used for algae Endothall – non-selective contact herbicide Aquathol has lower toxicity to animals than Hydrothol formulation, but is a poorer algicide Diquat – non-selective contact herbicide Low toxicity to fish but may be toxic to zoops Ineffective in muddy water Glyphosate - non-selective contact herbicide Low toxicity to fish but may be toxic to zoops Ineffective in muddy water Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s123 Herbicides – more chemicals Fluoridone – Systemic (kills entire plant) herbicide that is potentially selective based on concentration Requires extended contact time (>30 days) Typically applied to whole lake, not smaller areas Recommended for selective control of Eurasion watermilfoil midwest- mixed results because of difficulty in controlling lake-wide dosage 2,4-D – Systemic herbicide with some potential for selectivity based on dose and timing Time delays for recreation and agriculture Not for use in water supplies Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s124 Aquatic herbicide application www.ecy.wa.gov/ www.prolakemgt.com www.aquaticanalysts.com Developed by: Axler and Reed •aquat1.ifas.ufl.edu/-intro.html Updated: 11/26/03 Lakes U6-m24a-s125 Weed control – biological goals 7. Fish, insects or pathogens Goal: Consume or destroy vegetation; restore native species Usually involves introducing non-native species Fish - sterile grass carp used most often Insects - weevils and other species targeting Eurasian watermilfoil and other exotics Microbial pathogens – fungi, bacteria, viruses Largely experimental in aquatic systems except for fungal infection that may work well with herbicides Re-establish native species, especially in disturbed areas to restrict expansion of pest Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s126 Weed control – biological issues 7. Fish, insects or pathogens Major issues: Regulatory permits – grass carp often banned Unintended foodweb and vegetation alteration may make matters worse (overgrazing and nontarget consumption by carp if densities are too high) Usually not expected to eradicate a pest species Difficult to simulate ecosystem responses (control species may become pests) Integrated pest management involving combinations of control strategies may work best Re-establishing native plants and minimizing disturbance may be a good long-term strategy Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s127 Weed control – biological methods Triploid grass carp (Ctenopharyngodon idella): the most commonly used and effective biological control currently available. (www.aquat1.ifas.ufl.edu/mach2.jpg) Milfoil weevil (Euhrychiopsis lecontei): a native insect that has a preference for the exotic nuisance plant over its “natural” plant food; being used to help control the exotic Eurasian watermilfoil (www.fw.umn.edu/research/milfoil/milfoilbc.html) Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s128 Lakescaping Lakescaping is the process of restoring (re-vegetating) a shoreline to correct an erosion problem or to improve the fisheries and water quality of the lake or river At the heart of the lakescaping concept is the creation of a buffer zone along the shoreline. A buffer zone is a natural strip of vegetation along a property's frontage. Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s129 Lakescaping - principles • Emergent vegetation, e.g. bulrushes and cattails, reduce shoreline erosion caused by wind and boat traffic and reduce sediment resuspension • Plants provide a filter strip that helps absorbs lawn fertilizer and pesticide runoff before it reaches the lake • Buffer zones reduce lawn chemical usage because the resulting lawn is smaller, and native plants in the buffer zone do not need chemicals • Unmowed shoreline wildflowers, grasses and sedges are less hospitable to Canada geese- i.e. less bird feces Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s130 Aquatic plant control- field images Hydroraking www.aquaticanalysts.com Rototilling www.aquaticanalysts.com Developed by: Axler and Reed Lake Drawdown www.dnr.cornell.edu/ext/wetlands Clamshell Bucket Dredge www.aquaticanalysts.com Updated: 11/26/03 Lakes U6-m24a-s131 Developed by: Axler and Reed Updated: 11/26/03 Lakes U6-m24a-s132