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Cellular Division copyright cmassengale 1 Cell Division All cells are derived from pre- existing cells New cells are produced for growth and to replace damaged or old cells Differs in prokaryotes (bacteria) and eukaryotes (protists, fungi, plants, & animals) copyright cmassengale 2 Identical Daughter Cells Two identical daughter cells Parent Cell copyright cmassengale 3 Chromosomes copyright cmassengale 4 Prokaryotic Chromosome The DNA of prokaryotes (bacteria) is one, circular chromosome attached to the inside of the cell membrane copyright cmassengale 5 Chromosomes in Dividing Cells Duplicated chromosomes are called chromatids & are held together by the centromere Called Sister Chromatids copyright cmassengale 6 Karyotype A picture of the chromosomes from a human cell arranged in pairs by size First 22 pairs are called autosomes Last pair are the sex chromosomes XX female or XY male copyright cmassengale 7 Cell Reproduction https://www.youtube.com/ watch?v=9nsRufogdoI Mitosis and Meiosis song 3:50min. copyright cmassengale 8 Types of Cell Reproduction Asexual reproduction involves a single cell dividing to make 2 new, identical daughter cells Mitosis & binary fission are examples of asexual reproduction Sexual reproduction involves two cells (egg & sperm) joining to make a new cell (zygote) that is NOT identical to the original cells Meiosis is an example copyright cmassengale 9 Cell Division in Prokaryotes Prokaryotes such as bacteria divide into 2 Parent cell identical cells by the process of binary fission Chromosome Single chromosome replicates makes a copy of itself Cell wall forms Cell splits between the chromosomes dividing the cell 2 identical daughter cells copyright cmassengale 10 Animation of Binary Fission copyright cmassengale 11 The Cell Cycle copyright cmassengale 12 Five Phases of the Cell Cycle G1 - primary growth phase S – synthesis; DNA replicated G2 - secondary growth phase collectively these 3 stages are called interphase M - mitosis C - cytokinesis copyright cmassengale 13 Cell Cycle copyright cmassengale 14 Interphase - G1 Stage 1st growth stage after cell division Cells mature by making more cytoplasm & organelles Cell carries on its normal metabolic activities copyright cmassengale 15 Interphase – S Stage Synthesis stage DNA is copied or replicated Two identical copies of DNA Original DNA copyright cmassengale 16 Interphase – G2 Stage 2nd Growth Stage Occurs after DNA has been copied All cell structures needed for division are made (e.g. centrioles) Both organelles & proteins are synthesized copyright cmassengale 17 What’s Happening in Interphase? What the cell looks like Animal Cell What’s occurring copyright cmassengale 18 Sketch the Cell Cycle DNA Copied Cells Mature Daughter Cells Cells prepare for Division Cell Divides into Identical cells copyright cmassengale 19 Mitosis https://www.youtube.com/watch ?v=VlN7K1-9QB0 1.29..MIN. ANIMATION… copyright cmassengale 20 Mitosis Division of the nucleus Also called karyokinesis Only occurs in eukaryotes Has four stages Doesn’t occur in some cells such as brain cells copyright cmassengale 21 Four Mitotic Stages Prophase Metaphase Anaphase Telophase copyright cmassengale 22 Spindle Fiber attached to Chromosome Kinetochore Fiber Chromosome copyright cmassengale 23 Review of Prophase What the cell looks like What’s copyright happening cmassengale 24 Sketch The Spindle copyright cmassengale 25 Review of Metaphase What the cell looks like What’s occurring copyright cmassengale 26 Anaphase Sister Chromatids being separated copyright cmassengale 27 Anaphase Review What the cell looks like What’s occurring copyright cmassengale 28 Comparison of Anaphase & Telophase copyright cmassengale 29 Cytokinesis Cleavage furrow in animal cell Cell plate in plant cell copyright cmassengale 30 Daughter Cells of Mitosis Have the same number of chromosomes as each other and as the parent cell from which they were formed Identical to each other, but smaller than parent cell Must grow in size to become mature cells (G1 of Interphase) copyright cmassengale 31 Identical Daughter Cells What is the 2n or diploid number? 2 Chromosome number the same, but cells smaller than parent cell copyright cmassengale 32 Draw & Learn these Stages copyright cmassengale 33 Draw & Learn these Stages copyright cmassengale 34 Eukaryotic Cell Division Used for growth and repair Produce two new cells identical to the original cell Cells are diploid (2n) Prophase Metaphase Chromosomes during Metaphase of mitosis Anaphase Telophase Cytokinesis copyright cmassengale 35 Mitosis Animation Name each stage as you see it occur? copyright cmassengale 36 Mitosis in Onion Root Tips Do you see any stages of mitosis? copyright cmassengale 37 Test Yourself over Mitosis copyright cmassengale 38 Identify the Stages ? Early, Middle, & Late Prophase ? ? Metaphase Late Prophase Late Anaphase Anaphase ? ? Telophase copyright cmassengale ? ? Telophase & Cytokinesis 39 Locate the Four Mitotic Stages in Plants Anaphase Telophase Metaphase Prophase copyright cmassengale 40 Uncontrolled Mitosis If mitosis is not controlled, unlimited cell division occurs causing cancerous tumors Oncogenes are special proteins that increase the chance that a normal cell develops into a tumor cell copyright cmassengale Cancer cells 41 https://www.youtube.com/wa tch?v=JcZQkmooyPk MITOSIS…..6.20 MIN. 10 FACTS!!!!!! copyright cmassengale 42 Meiosis Gamete Production 43 Meiosis Similar in many ways to mitosis Several differences however Involves 2 cell divisions Results in 4 cells with 1/2 the normal genetic information. Vocabulary: Diploid (2N) - Normal amount of genetic material Haploid (N) - 1/2 the genetic material. 44 Meiosis Meiosis results in the formation of haploid cells. In Humans, these are the Ova (egg) and sperm. Ova are produced in the ovaries in females Process is called oogenesis Sperm are produced in the testes of males. Process is called spermatogenesis Meiosis occurs in 2 phases: Meiosis I & Meiosis II 45 Spermatogenesis & Oogenesis Sperm formation Egg formation 46 Meiosis I Prior to division (S phase), amount of DNA doubles 47 Metaphase I During Metaphase I homologous chromosomes lineup along the metaphase plate or EQUATOR Areas of homologous chromosomes connect at areas called CHIASMATA Genes are exchanged at these connections 48 Crossing Over Segments of homologous chromosomes break and reattach at similar locations. Results in new genetic combinations of offspring. 49 Crossing-Over Crossing-over multiplies the already huge number of different gamete types produced by independent assortment copyright cmassengale 50 Chiasmata 51 Anaphase I During Anaphase I, each HOMOLOGOUS CHROMOSOME is pulled to opposite sides of the cell. Unlike mitosis, the CENTROMERES DO NOT BREAK. Nuclei MAY OR MAY NOT reform following division. CYTOKENESIS may or may not occur. 52 Meiosis II DNA DOES NOT double Chromosomes randomly line-up along metaphase plate like regular mitosis. During Anaphase II, CENTROMERES BREAK and each SISTER CHROMATID is pulled to opposite sides of the cell. Nuclei reform and cytokinesis usually occurs (although it is often unequal). Sister chromatids 53 Meiosis: Two Part Cell Division Sister chromatids separate Homologs separate Meiosis I Meiosis II Diploid Diploid copyright cmassengale Haploid 54 Overview of Meiosis 55 Meiosis Animation copyright cmassengale 56 https://www.youtube.com/wa tch?v=16enC385R0w BOZEMAN…..8.25 MIN. 10 FACTS !!!!!!! https://www.youtube.com/wa tch?v=rqPMp0U0HOA Meiosis..crossing over 6:45 10 facts!!!!! copyright cmassengale 57 Comparison of Divisions Mitosis Meiosis 2 Number of divisions 1 Number of daughter cells 2 4 Yes No Same as parent Half of parent Where Somatic cells Germ cells When Throughout life At sexual maturity Genetically identical? Chromosome # Role Growth and repair copyright cmassengale Sexual reproduction 58