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Cell Membrane Passive Vs Active Transport Cell Unit Outline • Diffusion and osmosis • Active vs Passive transport • Mitosis and meiosis ACOS # 2 • Describe cell processes necessary for achieving homeostasis, including active and passive transport, osmosis, diffusion, exocytosis and endosytosis – Identify functions of carbohydrates, lipids, proteins, and nucleic acids – Compare reactions of plant and animal cells in isotonic, hypotonic and hypertonic solutions ACOS # 2 – Explain how surface area, cell size, temperature, light an pH affect cellular activities – Apply the concept of fluid pressure to biological systems; examples – blood pressure, turgor pressure, bends, strokes ACOS # 2 – Terms • Active transport – when the cell must use energy to move substances in or out • Bends – decompression sickness; caused by nitrogen gas under pressure • Concentration gradient – when there is a greater amount (of molecules) in one place than there is in another ACOS # 2 – Terms • Endocytosis – type of active transport when a cell brings large materials inside • Exocytosis – type of active transport when cells move materials to the outside • Diffusion – type of passive transport when molecule ‘spread out’ • Homeostasis – steady state; the process of maintaining correct conditions for life ACOS # 2 – Terms • Hypertonic – a concentrated solution • Hypotonic – a less concentrated solution • Isotonic – two solutions are in equal concentration • Osmosis – type of passive transport; when water diffuses down its concentration gradient ACOS # 2 – Terms • Passive transport – when the cell can use the energy of the molecules to move materials in or out and not its own energy • pH – unit measuring acidity (blood) • Surface area – the ratio of outside of a cell compared to the total volume of the cell ACOS # 2 – Terms • Stroke – rupture of a blood vessel (usually from high blood pressure) • Turgor – pressure created by water in the water vacuole of plant cells Cell Membrane • • • • Cell have to move materials in or out What do they move in - ? What do they move out - ? What structure controls what gets in or out? Cell Membrane • Controls what materials enter or exit the cell Passive Transport • PASSIVE TRANSPORT – the cell does not have to spend its energy for molecules to get in/out • Molecules are in constant motion • The energy from the motion may be enough to let some molecules ‘leak’ through the membrane • Molecules ‘want’ to ‘spread out’ Concentration Gradient • Molecules may be closer together in one area than they are in another • The difference between the concentration of molecules in one area vs the other area is called a: • CONCENTRATION GRADIENT Diffusion • Diffusion – when molecules move from where they are close together to spread apart • Whenever there is a CONCENTRATION GRADIENT, molecules will diffuse • The molecules will try to spread out • Molecules move from an area of high concentration to an area of low concentration Diffusion Types of Passive Transport • Diffusion – molecules ‘spread out’; move from an area of high concentration to an area of lower concentration • Small molecules diffuse through the membrane easily; – Gases like oxygen and carbon dioxide Diffusion • Molecules move from an area of high concentration into the area of lower concentration “down the CONCENTRATION GRADIENT” • Oxygen diffuses into blood cells from the lungs • Carbon dioxide diffuses from blood to the lungs Alveoli ALVEOLUS Oxygen CO2 Oxygen Lots of CO2 No O2 Oxygen CO2 CO2 CO2 CO2 CO2 CO2 Oxygen Lots of CO2 No O2 Oxygen CO2 CO2 CO CO2 2 Oxygen Lots of O 2; No CO2 CO2 CO2 Lots of CO2 No O2 Diffusion In Your Lungs • Carbon dioxide (CO2) DIFFUSES down its CONCENTRATION GRADIENT out of the red blood cells • Oxygen DIFFUSES down its CONCENTRATION GRADIENT into the red blood cells Diffusion In Your Body • In your body, all your body cells (muscles, skin, stomach, nerves) produce carbon dioxide and use up oxygen • Carbon dioxide DIFFUSES out of the body cells and into the blood cells • Oxygen DIFFUSES from blood cells into body cells Oxygen CO2 CO CO2 2 CO2 CO2 Oxygen Smoking prevents diffusion Passive Transport - Diffusion • The movement of molecules down a concentration gradient from an area of high concentration to an area of low concentration – A type of PASSIVE TRANSPORT because the molecules have enough energy to move across the membrane Passive Transport - Osmosis • A type of diffusion • Water molecules move down their concentration gradient from an area where there is more water to the area where there is less water Osmosis • Cells are a solution of sugars, proteins, minerals, salts, etc. • Cells are surrounded by a solution • The amount of water in the cell compared to the amount of water outside of the cell is the concentration gradient Solutions • Made of two parts: • Solute = substance that gets dissolved; ex. Sugar, salts, minerals; tea, kool-aid • Solvent = substance that does the dissolving, usually water • The amount of sugar (solute) in the water is the CONCENTRATION Osmosis • Three terms are used to describe the solution inside the cell compared to the solution outside the cell: – Hypertonic – Hypotonic – Isotonic Osmosis • Hypertonic = a solution that has MORE solutes than the other solution. Hyper = higher • Hypotonic = a solution that has LESS solutes than the other solution. Hypo - low • Isotonic = solutions that have EQUAL amounts of solutes ISOTONIC 1% 1% Water moves from the lower number into the higher number The solution is HYPOTONIC 0% 1% The cell is HYPERTONIC Water moves from the lower number into the higher number The cell is HYPOTONIC 1% 3% The solution is HYPERTONIC 1% Equilibrium 1% Water always moves from the area of high WATER concentration into the area of low WATER concentration Solution is____________ Cell = 1% salts 1% 99% water Solution = 3% salts 3% Size of the cell will… 97% water Solution is ______________ Cell = 1% salts 1% 99% water Solution – 0% salts 0% Cell will ……… 100 % water Osmosis • Water will move from HYPOtonic into HYPERtonic 1% 3% 1% 2% Hypertonic solution Plasmolysis Hypotonic solution Turgor pressure = water pressure placed on plant cell walls 1% 0% 1% 1% 1% 3% Osmosis Salt water fish 3% 1% salt Fresh water fish 0% 1% 0% -freshwater 1% salt 0% 0% salt 3% salt water 1% 3% salt water 3% Osmosis Passive Transport- Facilitated Diffusion • Some molecules are too big and need ‘help’ to fit through the membrane – Ex. Glucose • A special molecule embedded in the membrane acts as a ‘channel’ to let glucose in • INSULIN Glucose Glucose Glucose Insulin Transport protein Insulin Glucose Insulin Insulin Insulin Passive Transport: Review • Cell spends no energy for molecules to move inside • 3 types: – Diffusion – Osmosis – Facilitated diffusion Active Transport Cell Has to Use Energy To Move Substances Across The Membrane Active Transport • Why does the Cell have to use its own energy? 1. Must move particles AGAINST the concentration gradient 2. Particles are too LARGE Types of Active Transport • Endocytosis • Exocytosis Endocytosis • Large particles brought into the cell • TOO BIG • Cell membrane ‘pinches’ in Endocytosis/Exocytosis Phagocytosis Amoeba Endocytosis Endocytosis Endocytosis Endocytosis Phagocytosis Exocytosis • Large particles are moved to outside of the cell • Opposite of endocytosis • Two reasons: 1. Excretion – removal of wastes 2. Secretion – substance used by another cell (INSULIN) Exocytosis Cell Transport: Review • • • • Passive Diffusion Osmosis Facilitated diffusion • • • • Active Pumps Endocytosis Exocytosis