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CELL MEMBRANES
and
TRANSPORT
1
The Cell
2
Cell Membrane
All living things are surrounded by a
membrane.
 A cell membrane is also known as plasma
membrane.
 Controls exchange of materials such as
nutrients and waste between cells and their
environment.
 Has other important functions
for example to enable cells to receive
hormones.

3
Cell Membrane
 Fluid
Mosaic Model - proteins embedded
in a phospholipid bilayer.
4
Fluid mosaic model
FLUID- because individual phospholipids and proteins
can move around freely within the layer, like it’s a
liquid.
MOSAIC- because of the pattern produced by the
scattered protein molecules when the membrane is
viewed from above.
5
Cell membranes are made of
PHOSPHOLIPIDS

HYDROPHILIC heads
(water liking)
-Attracted to the water

HYDROPHOBIC tails
(water fearing)
-repel water
A Phospholipid
6
A Phospholipid Bilayer
Phospholipids can form:
BILAYERS
-2 layers of phospholipids
with hydrophobic tails
protected inside by the
hydrophilic heads.
The PHOSPHOLIPID
BILAYER is the basic
structure of membranes.
7
 Bilayer:

2 layers of phospholipids
The heads stay on the outside and the tails
stay on the inside.
8
Cell Membrane
 Cell



surface proteins
a. Channel proteins - transport food and
other molecules into the cell and transport
wastes out of the cells.
b. Receptor proteins - gather information
about the cell’s surroundings.
c. Cell surface markers - identify the type
of cell, important for cell recognition.
9
Movement of selected molecules
across the cell membrane
10
Cell Membrane
 Permeability


of the cell membrane
1. Semi permeable/selectively permeable only certain substances can pass across the
membrane.
2. Factors that determine whether a
molecule can pass through a membrane or
not:
• a. size
• b. type
11
Transport Mechanisms

Transport Mechanisms - moving material in and
out of the cell

Concentration gradient - the difference in the
amount of a substance inside and outside of the
cell



1. Going “with the gradient”-moving from high to low
concentration
2. Going “against the gradient”-moving from low to
high concentration
3. Equilibrium exists when the concentration of
molecules is the same throughout a space (inside and
outside the cell)
12
Transport Mechanisms
 Two
categories of transport based on
concentration gradient and the need for
energy

1. Passive transport does not require energy
• goes with the gradient.

2. Active transport requires energy
• goes against the gradient.
13
Methods of Transport
Across Membranes
1. Diffusion
2. Osmosis
3. Facilitated Diffusion
4. Active Transport
14
Methods of Transport
Across Membranes
Diffusion -passive transport - no
energy expended
2. Osmosis - Passive transport of water
across membrane
3. Facilitated Diffusion - Use of proteins to
carry molecules or ions across
4. Active Transport- requires energy to
transport molecules against a
concentration gradient – energy is
in the form of ATP
1.
15
Types of Transport
16
Passive Transport Mechanisms
Diffusion
Movement of molecules from an area of
high concentration to an area of low
concentration.
 Movement from one side of a membrane to
another
 Example: smoke across a room, food
coloring dropped into water, oxygen in
lungs

17
Diffusion
Diffusion in action
18
Passive Transport Mechanisms
Osmosis
 Diffusion


of water
Direction of osmosis-the direction of water
flow depends upon the concentration of solute
and solvent (water)
Tonicity-term used to compare 2 solutions
(usually the inside and the outside of the cell)
• a. Hypotonic - less solute (more water)
• b. Hypertonic - more solute (less water)
• c. Isotonic - equal amounts of solute and water
19
Passive Transport Mechanisms
Osmosis


Water will flow from a hypotonic
solution to a hypertonic solution
Will water move in an isotonic
solution?
• Yes, but it won’t be noticeableequilibrium is established
20
Osmosis
How Osmosis
works
21
Solutions
 Solutions
are made of solute and a
solvent
 Solvent - the liquid into which the
solute is poured and dissolved.
 Solute - substance that is dissolved
or put into the solvent.

Salt and sucrose are solutes.
22
Passive Transport Mechanisms

Turgor pressure/osmotic pressurepressure due to water in cell.
• Analogy: air pressure in a tire.


When a cell has high turgor pressure, it is
bigger and stiffer.
When a cell has low turgor pressure, it is
smaller and flimsy.
23
Passive Transport Mechanisms
 Plasmolysis
- wilting of a cell due to loss
of turgor pressure
 Cytolysis
- bursting of a cell due to an
increase in turgor pressure.


How do plant cells avoid cytolysis? Cell walls
How do unicellular freshwater organisms
avoid bursting? Contractile vacuoles.
24
Plant and Animal Cells put into
various solutions
25
Passive Transport Mechanisms
26
Passive Transport Mechanisms
27
Passive Transport Mechanisms
 Facilitated
diffusion - transport of
specific molecules across a membrane
with the help of a channel protein

An example of a molecule that is often
transported in this manner is glucose
How Facilitated Diffusion Works
28
Active Transport Mechanism
 Active
Transport Mechanism
(requires energy in the form of ATP)
 Ex.

Sodium-potassium pump - pumps sodium ions
out and forces potassium ions in.
• Important for conducting nerve impulses and
muscle contraction.

Proton pump-transport protons through
membranes of chloroplasts and
mitochondria
29
Active Transport Mechanism
 Endocytosis
- process of bringing larger
molecules into the cell by engulfing
them.


1. Pinocytosis – liquids
2. Phagocytosis - solids
• amoebas eat this way
• white blood cell engulf bacteria
30