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Who discovered cells? • Robert Hooke was the first person to use the term “cell” in 1665. • He was using a microscope to observe a thin slice of cork, he called the dead chambers he saw “cells”. • Cells vary considerably in size. The smallest cell, a type of bacterium known as a mycoplasma, measures 0.0001 mm (0.000004 in) in diameter; 10,000 mycoplasmas in a row are only as wide as the diameter of a human hair. Among the largest cells are the nerve cells that run down a giraffe’s neck; these cells can exceed 3 m (9.7 ft) in length. Human cells also display a variety of sizes, from small red blood cells that measure 0.00076 mm (0.00003 in) to liver cells that may be ten times larger. About 10,000 average-sized human cells can fit on the head of a pin. Prokaryotic cells • Smallest and simplest cells. • Single-celled organism that lacks a nucleus and other internal compartments. • Cannot carry out many specialized functions. • First lived 3.5 billion years ago. Example: bacteria Eukaryotic cells • Ten times larger than prokaryotic cells. • Organism whose cells have a nucleus. • Specialized • Found 1.5 billion years ago. • Some are single – celled. The Cell Theory • Formed after Schleiden, Schwann, and Virchow’s observations of cells. • Has three parts: 1. All living things are made of one or more cells. 2. Cells are the basic units of structure and function in organisms. 3. All cells arise from existing cells. 2 Types of Eukaryotic Cells • ANIMAL PLANT Organelle • Membrane bound compartments inside cells. • A structure that carries out specific activities in the cell. Cytoplasm Semifluid that fills the cell. Composed of about 65 percent water, the cytoplasm is packed with up to a billion molecules per cell, a rich storehouse that includes enzymes and dissolved nutrients, such as sugars and amino acids. Cytoskeleton • Microscopic fibers. • Crisscrosses the cytoplasm, anchoring the organelles in place and providing shape and structure to the cell. Cell Membrane / Plasma membrane All living cells have a cell membrane that separates them from their surroundings, much like the walls of this room separate us from the rest of the school. Characteristics of the Cell Membrane 1. Made up of lipids and proteins called a phospholipid bilayer. 2. Acts as a barrier. 3. Controls what enters and leaves the cell. (Selective permeability) 4. Sometimes called the fluid mosaic model because it is not rigid. The Cell Membrane The Nucleus • Controls almost all cell functions. • Surrounded by a porous double membrane called the nuclear membrane or envelope. • Large, dark structure. The Nucleolus • Found inside the nucleus. • Composed of RNA and proteins that are involved in ribosome formation. • It can be thought of as the brain because it contains the genetic material. Ribosomes • Tiny bead like structures. • Found in both prokaryotes and eukaryotes. • Manufacture proteins. • Composed of both RNA and proteins. Endoplasmic Reticulum • 2 types: 1. Rough ER 2. Smooth ER • Serves as a transport system by moving proteins and other substances through the cell. • The rough ER has ribosomes located on its surface and the smooth ER does not. Rough ER The Golgi Apparatus • Vesicles that contain newly made proteins. • Modifies, collects, packages and distributes molecules after they are made at the ER and other cell locations. • Flattened, membrane bound sacs. The Golgi Apparatus Lysosomes • Small, spherical organelles that contain the cells digestive enzymes. • They act as a clean up crew. • They are like the toilet of the cell because they digest waste products. Vacuoles • Store materials like water, salt proteins, and carbohydrates. • Plant cells have a very large central vacuole that is filled with fluid. Mitochondria • Change the chemical energy stored in food into compounds the cell can use. • Makes almost all of the cells ATP. • Sometimes called the powerhouse of the cell. Chloroplasts • Found only in plant cells and algae. • Use light energy (sun) to make carbohydrates from carbon dioxide and water that the mitochondria can use. • Chloroplasts and mitochondria supply the energy needed to power plant cells. • Where photosynthesis takes place. Chloroplast in onion cells: • Chloroplasts, minute green, spherical structures, are essential to the process of photosynthesis, in which captured sunlight is combined with water and carbon dioxide in the presence of the chlorophyll molecule to produce oxygen and sugars, which can be used by animals. Without the process of photosynthesis, the atmosphere would not contain enough oxygen to support animal life. ANIMAL VS. PLANT CELLS ANIMAL PLANT Cell membrane Cell wall and cell membrane. Large central vacuole. Chloroplasts that give plants a green color. PLANT CELL ANIMAL CELL Cell Specialization • Occurs when cells change to perform certain functions better. • Sometimes called complex multicellularity. Ex. Rod and Cone cells in your eye are specially adapted to allow you to see colors. Cellular Organization 1. Cells – nerve cells, red and white blood cells. 2. Tissues – distinct group of cells with similar structure and function. Ex. Muscles 3. Organs – tissues organized into a specialized structure with a specific function. Ex. Heart 4. Organ System – various organs that carry out a major body function. Ex. Circulatory system Cells and Homeostasis • Homeostasis – when organisms make internal adjustments based on external changes. • One way cells maintain homeostasis is by controlling the movement of substances across their cell membranes. • Sometimes this movement requires energy (active transport) and sometimes it does not (passive transport). PASSIVE TRANSPORT • NO ENERGY REQUIRED! • 2 Types 1. Diffusion – movement of substances from an area of higher concentration to one of lower concentration in an attempt to reach equilibrium. 2. Osmosis – diffusion of water molecules through a selectively permeable membrane. ACTIVE TRANSPORT • REQUIRES ENERGY! • Particles move against the concentration gradient. • Involves carrier proteins found in the cell membrane that act as “elevators” or “carriers” of the material into the cell. Antoni van Leeuwenhoek • Improved the simple microscope. • Built over 240 microscopes. • Discovered living creatures in pond water which he called “animalcules”. They were actually single-celled organisms. • 1st to discover and describe bacteria. MICROSCOPES • 2 common kinds: 1. Light microscope – light passes through one or more lenses to produce an enlarged image of a specimen. 2. Electron microscope – forms an image of a specimen using a beam of electrons rather than light. Compound Microscope • Light microscope that uses two lenses. • Used to view living and small organisms. Maximum magnification is 2000x YOU NEED TO BE ABLE TO IDENTIFY ALL THE PARTS OF A COMPOUND MICROSCOPE! Compound Microscope Calculating magnification • Multiply the power of the eyepiece (ocular) by the power of the objective being used. Ex. Eyepiece 10x Objective 40 x TOTAL MAGNIFICATION 400X The Electron Microscope • 2 Types 1. Transmission (TEM)– electron beam is directed at a very thin slice of specimen that has been stained. 2 dimensional picture. Magnify up to 200,000x. Always black and white. 2. Scanning (SEM)- 3 dimensional image of the specimens surface only. Specimen is coated in a thin layer of metal. Magnify up to 60,000x.