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Practical 1 NEXT WEEK??? Aghhhhhh!!! Study Tips Review your quizzes Know material on handouts Multiple choice Short answer Fill-in-the-blank Goals for Today’s Lab 1) Diversity of cells 2) Function of cell organelles 3) The Cell Cycle What is a Cell? Cells are the building blocks of the body Form the tissues There’s lots of different kinds of cells…. The Diversity of Cells Epithelial Cells Red Blood Cells Skeletal muscle cells Cartilage cells Gametes Cell Anatomy – The Plasma Membrane Separates the cell from outside environment Selective permeability Example: H2O in/out easily Glucose not as easily Cell Anatomy - Cytoplasm Contents of cell outside the nucleus Contains the organelles Cellular Anatomy - Nucleus Control center of the cell Contains the DNA Surrounded by nuclear membrane The Organelles Machinery of the cell Enable cells to perform functions Ex: produce ATP, proteins Kitchen Cell Nucleus Organelles Organelles – The Ribosome Location of protein synthesis Free-floating or attached to endoplasmic reticulum (Rough ER) Organelles – Endoplasmic Reticulum Rough ER stores and transports proteins made on ribosomes Organelles – The Golgi Apparatus Flattened sacs that package proteins for export out of the cell. Organelles – Lysosomes Contain digestive enzymes Digest foreign substances entering the cell Organelles – The Peroxisome Contains the catalase enzyme Controls the accumulation of H2O2 in body Organelles – Mitochondria Powerhouse of the cell Produces ATP through breakdown of glucose LAB ACTIVITY 1 Look at electron micrograph of cell - Identify cell structures and organelles Why do Cells Reproduce? 1. Growth 2. Replace old or damaged cells Ex: Sunburn How Often do Cells Divide? It depends on the tissue Some Constantly Divide 1) Skin Cells 2) Stomach Lining Some Almost Never Divide 1) Nerve Cells 2) Muscle Cells The Cell Cycle Interphase • Most of a cell’s life • DNA in long strands called “chromatin” • Nuclear envelope intact • DNA replication occurs during “S Stage” Mitosis • Division of the nucleus • One cell forms 2 identical daughter cells • 4 stages i) Prophase ii) Metaphase iii) Anaphase iv) Telophase i) Prophase • Chromatin shortens into chromosomes • Chromosomes are 2 threads called “Sister Chromatids” joined at centromere Centromere i) Prophase, Cont. •Nuclear envelope breaks apart •Sister chromatids migrate to center of cell ii) Metaphase • Sister chromatids line up along the “metaphase plate” • Mitotic Spindle iii) Anaphase • Centromere of sister chromatids break • Each chromosome is pulled to opposite ends of the cell by mitotic spindle iv) Telophase • Chromosomal movement stops • Chromosomes uncoil into thin chromatin • Nuclear envelope forms • Cytokinesis Cytokinesis • Division of the cytoplasm • “Cleavage furrow” squeezes cells apart • Formation of two identical daughter cells Daughter Cell Cleavage Furrow Daughter Cell Mitosis vs. Meiosis vs. Cytokinesis Mitosis: Division of the NUCLEUS in the cell Meiosis: Division of GAMETES Cytokinesis: Division of CYTOPLASM at the end of Mitosis Which stage of Mitosis are these cells in? LAB ACTIVITY 2: Identify the different stages of mitosis on the cell slides under microscope LAB ACTIVITY 3: Interactive Genetics 1. 2. 3. 4. 5. 6. 7. 8. 9. Open up the C Drive Go to “Program Files” folder Open up “Interactive Genetics” folder Double click on “Start.exe” file Program should now open….. Click on Topic II Click on “Mitosis & Meiosis Do Mitosis Movie Do Problem 1