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Chapter 1 Topic 3 The cell membrane & the cell wall Chapter outline – The cell membrane • The cell membrane regulates the flow of materials into and out of a cell. • It is selectively permeable, meaning that only certain materials can pass through the membrane. • A cell membrane consists of a bilayer of phospholipids with protein molecules jutting through one or both layers. The cell wall – Prokaryotic cell wall • The prokaryotic cell wall consists of long polymers called peptidoglycans. • Some prokaryotic cell walls have a second, outer layer composed of lipoproteins and lipopolysaccharides. – Eukaryotic cell wall • The eukaryotic cell wall has three parts: the primary cell wall, the middle lamella, and the secondary cell wall. • The primary cell wall, composed mainly of cellulose, is the innermost layer of the wall. • The middle lamella contains polysaccharides called pectins. • The secondary cell wall is the outermost layer. It consists of cellulose and a strengthening material called lignin. Terms to know • • • • • • • • • • • • active transport - Transport in which a cell must expend energy. Active transport occurs against a concentration gradient. basal body - A structure found at the connection of cilia and flagella with the cell membrane. It is composed of microtubules in a circular configuration of nine triplets. cell membrane - The structure which surrounds the cell and regulates the movement of materials into and out of the cell. It is composed mostly of phospholipids. cell wall - A structure found in most prokaryotes and some eukaryotes which gives the cell greater structure. In prokaryotes, it is composed of peptidoglycans, and in eukaryotes, it consists or polysaccharides, pectins, and lignin. cilia - Tiny hairs along the outside of the cell membrane which are used to move the cell and capture food particles. chloroplast - The organelle in which photosynthesis takes place. It contains chlorophyll. chromoplast - An organelle in which photosynthesis take place. It contains pigments other than chlorophyll, resulting in a color other than green. contractile vacuole - An organelle which pumps excess water of a cell to prevent it from bursting. endoplasmic reticulum - A network of tunnels which extend away from the nucleus, used for the transport of proteins. facilitated diffusion - A method of transport across the cell membrane by which carrier proteins bond to a molecule on one side of the membrane, move through the membrane, and then release it on the other side. flagella - Large hairs which can whip back and forth to propel a cell. Golgi body - Stacks of membranous pouches which act as a transport station, packaging proteins from the endoplasmic reticulum and placing them into tiny vesicles. Terms to know continued…. • • • • • • • • • • • • • • • intermediate filament - A part of the cytoskeleton with a strong, ropelike structure which gives the cell strength and helps it to maintain its shape. leucoplast - Colorless plastids in autotrophs which store starch, proteins, and lipids. lysosome - A sac similar to a vacuole which contains powerful digestive enzymes used to break down large food particles. microfilament - A part of the cytoskeleton which consists of actin and aids in cell movement. microtrabeculae - Tiny fibers which interconnect all of the structures within the cell and help to give the cell shape. microtubule - Fibers which extend from the center of the cell to the cell membrane. They are involved in cell reproduction and are part of the composition of cilia and flagella. mitochondria - The organelle in which cellular respiration occurs. nucleolus - A structure within the nucleus at which ribosomes are created. nucleus - The organelle in eukaryotes which contains the cells DNA and thus indirectly controls protein production and the rest of the cell. passive transport - A form of transport which allows highly polar molecules to move through the cell membrane without the expenditure of energy. This may occur either through protein channels or facilitated diffusion. peroxisome - An organelle similar to a vacuole which contains oxidizing enzymes which can help neutralize toxic substances. pseudopod - Extensions of the cytoplasm toward which the rest of the cytoplasm tends to flow. Pseudopodia can be used for movement and the capture of prey. ribosome - Structures found mainly in the endoplasmic reticulum whose function is to synthesize protein based upon the code of a messenger RNA molecule. vacuole - Membrane-bound sacs within a cell used to hold food particles, water, etc. vesicle - A tiny vacuole, often used to carry protein molecules packaged at the Golgi bodies. Plasma Membrane • The plasma membrane is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell • The general structure of a biological membrane is a double layer of phospholipids Animation: Membrane Selectivity Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings The fluid-mosaic model of the cell membrane Outside of cell (a) TEM of a plasma membrane Fig. 6-7 The plasma membrane Inside of cell 0.1 µm Carbohydrate side chain Hydrophilic region Hydrophobic region Hydrophilic region Phospholipid Proteins (b) Structure of the plasma membrane Extracellular components and connections between cells help coordinate cellular activities • Most cells synthesize and secrete materials that are external to the plasma membrane • These extracellular structures include: – Cell walls of plants – The extracellular matrix (ECM) of animal cells – Intercellular junctions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Cell Walls of Plants • The cell wall is an extracellular structure that distinguishes plant cells from animal cells • Prokaryotes, fungi, and some protists also have cell walls • The cell wall protects the plant cell, maintains its shape, and prevents excessive uptake of water • Plant cell walls are made of cellulose fibers embedded in other polysaccharides and protein Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings • Plant cell walls may have multiple layers: – Primary cell wall: relatively thin and flexible – Middle lamella: thin layer between primary walls of adjacent cells – Secondary cell wall (in some cells): added between the plasma membrane and the primary cell wall • Plasmodesmata are channels between adjacent plant cells Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Middle lamella 1 µm Central vacuole Cytosol Plasma membrane Plant cell walls Plasmodesmata Fig. 6-28 Plant cell walls Secondary cell wall Primary cell wall STOP !!! Don’t overworked The Extracellular Matrix (ECM) of Animal Cells • Animal cells lack cell walls but are covered by an elaborate extracellular matrix (ECM) • The ECM is made up of glycoproteins such as collagen, proteoglycans, and fibronectin • ECM proteins bind to receptor proteins in the plasma membrane called integrins Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 6-30 Extracellular matrix (ECM) of an animal cell Collagen Proteoglycan complex EXTRACELLULAR FLUID Polysaccharide molecule Carbohydrates Fibronectin Core protein Integrins Proteoglycan molecule Plasma membrane Proteoglycan complex Microfilaments CYTOPLASM ASSIGNMENTS. Due on 29th June 2011 before 5 pm. One of the functions of the cytoskeleton in animal cells is to give shape to the cell. Plant cells have a fairly rigid cell wall surrounding the plasma membrane. Does this mean that a cytoskeleton is unnecessary for a plant cell? Defend your answer in terms of other functions of the cytoskeleton. Functions of the ECM: Support Adhesion Movement Regulation Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Intercellular Junctions • Neighboring cells in tissues, organs, or organ systems often adhere, interact, and communicate through direct physical contact • Intercellular junctions facilitate this contact • There are several types of intercellular junctions – – – – Plasmodesmata Tight junctions Desmosomes Gap junctions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Plasmodesmata in Plant Cells • Plasmodesmata are channels that perforate plant cell walls • Through plasmodesmata, water and small solutes (and sometimes proteins and RNA) can pass from cell to cell Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 6-31 Plasmodesmata between plant cells Cell walls Interior of cell Interior of cell 0.5 µm Plasmodesmata Plasma membranes Tight Junctions, Desmosomes, and Gap Junctions in Animal Cells • At tight junctions, membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid • Desmosomes (anchoring junctions) fasten cells together into strong sheets • Gap junctions (communicating junctions) provide cytoplasmic channels between adjacent cells Animation: Tight Junctions Animation: Desmosomes Animation: Gap Junctions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 6-32 Intercellular junctions in animal tissues Tight junction Tight junctions prevent fluid from moving across a layer of cells 0.5 µm Tight junction Intermediate filaments Desmosome Gap junctions Space between cells Plasma membranes of adjacent cells Desmosome 1 µm Extracellular matrix Gap junction 0.1 µm See U Next Class !!!