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Abstracts of Papers Results: SgA concentration correlated with the severity and nature of AHS. The SgA concentration reduced twice at the disease height: in the mild cases 3.0±0.2 mg/ml, in the moderate cases 2.3±0.5 mg/ml, in severe cases 2.1±0.3 mg/ml compared with healthy children. During recovery indices were significantly lower than the norm 5.0±0.3 mg/ ml in mild cases, 4.6±0.3 mg/ml in moderate cases and 3.9±0.2 mg/ ml in severe cases. Concentrations of lysozyme (mild cases 22.1±0.1 mkg/ml in the period of stomatitis appearance and 66±0.1 mkg/ml during recovery; moderate cases 19±0.2 mkg/ml and 48±0.1 mkg/ ml respectively) reflected severity of the disease. Conclusion: Local immunity indices in the oral cavity makes the use of local immunocorrectors in complex AHS therapy pathogenetically reasonable. 5. POSTERS WITHOUT DISCUSSION PND1. Dental caries among 12-year-old children in northern Poland. K. Emerich*, B. Adamowicz-Klepalska. Dept. Paediatric Dentistry, Medical University Gdansk, Poland Aim: In 1987, 1995, 2003 and 2007 dental surveys were carried out in the Gdansk region of Poland investigating the prevalence of dental caries in 12-year-old school children. Method: Sampling and examination were performed according to WHO standards. A sample of 180 children in 1987, 1995, 2003 were selected from different living environments of a city and rural area around the city. In 2007 a sample of 300 children was examined. Results: The caries prevalence (% of caries-free children) and caries experience (mean DMFT scores) changed in some groups significantly in the period from 1987-2007. However, there was big change in caries experience in children living in a rural area. The average DMFT score of all 12-year-olds dropped from 4.07 in 1987 to 3.75 in 2007. Conclusion: Because of scarce public resources for oral health care and current caries trends in Poland, a national health policy emphasizing prevention rather then curative care is needed. PND 2. DMF structure in 12-year-old children in Belgrade elementary schools. V. Zdravkovic*, J. Tekic, G. Puletic-Lukic, et al. Colgate Palmolive Adria, Dental School, Centre of public health care, Belgrade, Serbia. Aim: This was to determine a DMF structure and a state of oral health among 5thgrade students attending elementary schools in Belgrade, Serbia. Method: A parallel analysis of epi-emiological data obtained during two research periods: in 1999 and in 2007. A systematic check-up of the 5th-graders from Belgrade elementary schools was utilized as a research instrument. During a check-up, a dental probe and a dental mirror were used, under a reflective lighting. An evaluation was done, based on a parallel analysis, a value of static coefficient of DMF structure index and a progression of tooth decay in both generations of the fifth graders. Results: DMF structure among the 5th-graders in 1999 was: Among 427 children, of both genders, subjects of a check-up, ages 11-12 (attending the fifth grade), out of 11,524 teeth present at the time, affected by caries were 321 (24.54), extracted teeth 237 (18.1%), teeth with fillings 750 ( 57.3%). There were 1308 DMF. 2007. Among 834 children, of both genders, subjects of a check-up, ages 11-12 (attending a 5thgrade), out of 22,527 teeth present at the time, affected by caries were 596 ( 20.9%), extracted teeth 638 (22.3%), teeth with fillings 1622 ( 56.89%). There were 2856 DMF. Conclusion: The state of oral health comes as a result of improper application of hygienic, dietary and health education measures at this age. Continual implementation of health education measures and early treatment of caries on the first permanent molars is a priority when work 54 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 PND 3. Checkup: Oral and dentalat 7-years old urban and rural children. T. Saulic, et al.. Faculty of Medicine in Podgorica, Public Health Institution Podgorica, Montenegro. Aim: This was to determinate temporary teeth DMFT structure and to assess if there was distinction in caries dispersion in urban and rural areas. Method: A cross-sectional study was performed in October-November 2005. It included the checkups of 988 6 year old children (300 from rural and 688 from urban areas). For the elaboration of the data oral health indices and statistical test were used. Results: Average number of teeth with caries per a examinee for children of this age was DMFT-1,95. Children in urban areas had lower values DMFT-1.5 in relation with children in rural areas DMFT-2.09. Percentage of teeth with caries were higher of children in rural areas D-95.3% in relation with children in urban areas D-90.2%. As for filled teeth percentage is higher of children in urban areas D-9.7% in relation with children in rural areas D-4.7%. Conclusion: Children in rural areas had caries more often than children in urban areas and higher percentage filled teeth with caries than children in urban areas. Importance of nowdays preventive measures and programs needs to be underlined in order to improve and maintain oral health state. PND 4. Caries prevalence of First Permanent Molars in Children. E.S. Boyarkina*, L.P. Kiselnikova. Moscow State University of Medicine and Dentistry, Russia. Aim: This was to study intensity, clinical course features of fissure caries in children at the age from 6 to 12 years old. Method: 313 children were clinically examined at the age from 6 to12, according to the WHO criteria. Results: DMF (mean) was 2.7. Decayed teeth prevailed (D=.2, F=0.5, M=0). Fissure sealants were revealed in 5% children. Intensity level of fissure caries was very low at the age of 6 (DF=0.9 (D=0,,7; F=0.2)) and was mean at the age of 12 (DF=2.9 (D=2.3; F=0.0.). By the age of 12 more often caries occurred in central and distal pits (54.4% and 36.8%), on all fissures of occlusal surface (24.2%) of upper М1; on all fissures of occlusal surface (72%), distal pit (2.8%) and buccal pit (29%) of lower М1. Conclusions: 1. High caries affection level was revealed, the first role in its structure played caries lesion of first permanent molars (it more often took place in central and distal pits of upper and lower М1). By the age of 12 caries extended on all fissures of occlusal surface. 2. Preventive resin restoration is indicated on early stages of fissure caries to preserve unaffected tooth tissues as much as possible. PND 5. Longitudinal study of oral health in pre-school children: three years study of caries experience. R. Ivančaková1*, Z. Broukal,2, E. Lenčová2, et al. 1Dept.Paed. Den- tistry, Faculty of Medicine, Univ. Hosp., Hradec Králové. 2Institute of Dent. Research, Prague, Czech Republic. Aim: To collect basic knowledge on factors influencing primary prevention and caries experience in pre-school children. Method: Caries experience, dental status, oral hygiene and salivary levels of cariogenic streptococci were examined in the 3 years prospective study among older pre-school children in relation to the dynamic of caries increment and with regard to the educational level of their mothers. Criteria for including the child in the study: informed consent of parents, no systemic disease of the child. Children were examined using standard methodology recommended by WHO. Parameters calculated: % caries free, dt, dmft, ri, sci, presence of dental plaque on upper incisors. Results: Mean caries increment between second and third year of study did not differ significantly amounting to 0.3 Abstracts of Papers new decayed teeth per year however it was highest among children caries free in the onset of study. Higher caries experience significantly positively correlated with the presence of dental plaque and in the final of study also with the salivary levels of cariogenic streptococci. Mothers of caries free children assigned significantly higher educational level than those of children with tooth decay and on the contrary the proportion of children at need of restorative care was highest among mothers with elementary education level. Conclusion: There is a need of effective primary preventive intervention in pre-school children. The educational level of mothers can be taken as one of important causes of the inequalities in oral health of children. Supported by Grant No. NR/8331-3, Grant Agency, Ministry of Health, CZ. PND 6. Dental caries in permanent teeth of children, living in the area of endemic fluorosis. S. S. Bogomolova , L. P. Kiselnikova, Dept of Children’s Therapeutic Stomatology, SMSU, Moscow, Russia Aim: This was to study the prevalence and the intensity of dental caries in permanent teeth of children, living in the area of endemic fluorosis. Method: A random sample was selected of 25 children, aged 7 to 10 yrs of age, living in the area which contains high concentration of fluoride (F) in water supply (Moscow Region, the city of Odintsovo). F-concentration for this city is 3.5 mg/L. Results: Examination of 25 children, aged 7 to 10 yrs revealed different forms of dental fluorosis (13 children had very mild fluorosis, 9 – mild fluorosis, and 3 children had severe fluorosis). The prevalence of caries was 76 % (19 persons of 25 in total). According to the grading of the World Health Organization such prevalence of caries (31-80 %) is considered as mild. The caries DMFT index was 2.32. Examination of the state of permanent teeth of children, living in the area of endemic fluorosis revealed that tooth decay occurrence was much more frequent for permanent molars. Localization of caries was observed on the occlusal, vestibular surfaces and on the crown tubercles as well. Conclusion: The destructive form of dental fluorosis accompanied by the tooth decay is very common for permanent teeth of children living in the area of endemic fluorosis PND 7. Early age children caries and its’ complications. A. V. Tokareva*, L. P. Kiselnikova, MSMSU, Children Clinic “Dental Fantasy”, Moscow, Russia. Aim: To evaluate the caries treatment in early age children. Method: Examination and treatment under GA of 25 children in the age 1.5 – 4.5 yeas including 9 children in the age of 1.5 – 3 years and 16 children of 3.1 – 4.5 years were carried out on the bases of Clinic “Dental Fantasy”. Results: Maxillary incisors and first molars caries among children in the age of 1.5-3 years. Index dmf composed 6.5. Upper incisors, canines, the first and the second upper and lower molars caries was revealed among children in the age of 3.1-4.5 years. Index dmf equals to 13.5. During treatment of children in the age 1.5 – 4.5 yeas conditions in 62% of cases restorations of teeth on caries and its’ complications occasion. Endodontic treatment was required in 25% of cases and in 13% of teeth were extracted. During treatment among children of 3.1 – 4.5 years teeth restorations were done in 67%, endodental treatment was carried out in 62% of cases including 83% by extirpation method and in 17% of cases pulp amputation was done. 33% of affected teeth were extracted on the occasion of chronicle periodontitis. Conclusion: A large requirement is for restoration measures and prosthetic dentistry is required during the period of formed temporary occlusion. PND 8. Characteristics of Children Attending Emergency Dental Clinic at a Hospital in Jeddah, Saudi Arabia. M. Al-Malik*, R. Al- Zohairy, Dental Dept., Armed Forces Hospital, Jeddah, Saudi Arabia. Objective: This was to investigate the characteristics of children presenting for emergency dental care at Armed Forces Hospital, Jeddah. Method: Data were collected over 4 weeks period for all children under 15 years old visiting the centre: day of presentation, session of the day, sex, age, medical history, source of referral, diagnosis and treatment provided. Results: 192 children attended the centre during the period of the study (93 male, 99 female). Number of children visiting the hospital was higher on Saturday, which is the first day of the week (50 patients). The largest percentage of patients attended the morning sessions (92%). 42% were patients attending for the first time whereas 57% attended as follow-up. 85% were self refereed compared to 15% refereed by others clinics. Main reason for visiting the clinic was caries (79%). 69% received definitive treatment for their dental problem. Conclusion: There is a need to educate parents for the importance of early attendance of the child to the dentist to start preventive measures and early treatment when necessary is stressed. PND 9. The complications of untreated caries and pulpar infections in children of Kosova. T. Ademaj*, et al.. Dept Pediatric Dent, Dent School, Med Faculty, Univ Prishtina Aim: To determine the prevalence of complications of untreated caries in children of Kosova. Method: The examination included 707 children, 541 school and 166 preschool children. The dental examination was done in the dental offices of three primary schools and of one kindergarten. The periapical changes were assessed using retroalveolar radiographic images. The dental examination was based on the criteria of World Health Organization for oral health assessment. Results: The mean age of the children was 9.7 ± 3.4 (mean ± SD). The mean of the teeth present was 24.0 ± 2.2. Mean dmft was 6.4 for preschool, and DMFT 5.8 for school children. The prevalence of complications as a result of untreated caries and pulpar infections was 17.9%. The commonest complications were: apical chronic periodontitis with or without fistula, pulpar necrosis and gangrene, abscess, phlegmona, etc. The complications were found more in the mandibula (54.3%), than in maxilla (45.7%). The tooth most affected with complications of the permanent dentition was the first molar (66.1%), and of the primary dentition was the second molar (41.6%). The Oral Hygiene Index showed the presence of the dental plaque in 64% of the children. Conclusion: The consequences deriving from the untreated caries and pulpar infections in our children are very grave. The teeth affected with these complications are usually extracted, due to the lack of treatment of the caries and pulpar pathologies. There is no preventive measures undertaken to prevent caries. PND 10. Application of radiological methods in diagnosis and treatment of pulpitis in temporary teeth. L. P. Kiselnikova*, O. S. Kovylina, I. Scherbina. Moscow State Univ. of Medicine and Dentistry, Moscow, Russia. Aim: To examine the possible applications of radiological methods in diagnosis and treatment of pulpitis in temporary teeth. Method: This method has been used during the examination of 255 temporary molars with children aged 4 to 7. Normally in intact temporary teeth an optical density of bone tissue is not lower than 192 units, according to the results of densitometry. Results: These data of 55 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 Abstracts of Papers densitometry have been used to determine the indications for optimal treatment methods in pulpitis of temporary teeth. We recommend to use pulpotomy for treatment of pulpits of multiroot teeth with developed roots; without physiological resorbtion and limited between 192 till 128 units of optical density of the initial densitometric index in the area of bifurcation. According to our data the number of complications after pulpitis treatment using the method of pulpotomy is 3.5% (2 years after treatment). We recommend to use method of devitalized amputation with the following mummification of the crown pulp for treatment of pulpits of multiroot teeth with developed roots and limited between 128 till 64 units of optical density of the initial densitometric index. The number of complications after 2 years is 2%. We recommend to use pulpectomy for treatment one root teeth and pulpitis of multiroot with the initial densitometric index of optical density under 64 units in the explored area. The number of complications after 2 year is 0.7%. Conclusion: We recommend using densitometry to determine the degree of changes in periodontal tissue and determine the indications for optimal treatment methods in pulpitis of temporary teeth PND 11. The frequency and patency of accessory canals in furcation areas of primary molars. H. S. Noh*, T. S. Jeong H. S. Lee, et al., Dept Pediatric Dentistry, School of Dentistry, Pusan National University, Pusan, Korea. Aim: This was to investigate the morphological characteristics of accessory canals at pulpal floor and furca areas of primary molars including their frequency and patency, which plays as connecting channel and is known as the main etiology of complicating the pulpal diseases in children. Method: 40 primary molars without any resorption of furca area were selected and sectioned for the specimen suitable to evaluate the accessory canals from pulpal floor, furca area and longitudinally. These were observed under SEM to investigate the frequency. And their patency as a connecting channel was evaluated after dye penetration with vacuum pressure by observing under stereo-microscope. Results: In SEM study, the frequency of accessory foramina viewed from the furca and pulpal floor was 60% and 35% respectively. Likewise the diameter of the accessory canals at orifice area was within the range of 11~107㎛ and 12~62 ㎛ respectively. The frequency of patent accessory canals connecting between pulp and furca evidenced by dye penetration was 50% among the 20 specimen teeth tested. Conclusion: The accessory canals at furca area seemed to play a significant role in pulp-furca combined pathoses in primary molars. PND 12. Microbiological assessment of polymer & conventional carbide bur in caries removal: in vivo. A. Ölmez*, E. Erdemli, G. Akça, et al. Dept. Paediatric Dentistry, School of dentistry, University of Gazi, Ankara, Turkey. Aim: This was to study the microbiological effectiveness of a polymer and conventional carbide bur in caries removal. Method: Participants consisted of 24 patients (16 boys, 8 girls) aged 5 to 9 years that presented with two carious lesions on the occlusal surfaces of primary molars requiring no access drilling throughout enamel. One of the primary molars was excavated with smartbur, other carious primary molar with carbide bur in the same appointment. Sample taking and caries removing have been done in this way; (1) first sample (from the carious dentine), (2) caries removal (by smartbur or carbide bur), (3) second sample (from the caries-free dentine), respectively. After caries removal, restorative procedures were finished. For microbiological procedures, Tryptone-yeast extract-cysteine-sukrozbacitracin agar for the isolation of mutans streptococci (MS), Rogosa Lactobacilli Selective Agar for the isolation of lactobacilli, blood agar for the isolation of aerob microorganisms and Schaedler agar for the 56 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 isolation of anaerob microorganisms were used. After counting the colonies on the agar plates, number of CFU/ml was calculated. Results: There were statistically significant differences in the numbers of CFU between before and after preparation for both types of burs (p<0.05).There were no statistically significant differences between the numbers of CFU in the carious dentin, comparing the polymer and carbide bur group for all the media used (p>0.05). Conclusion: The polymer bur is as effective as the conventional bur microbiologically in caries removal PND 13. Microstructure of enamel and dentine in primary teeth after the preparation with different burs. T. V. Fedulova*, L. P. Kiselnikova, E. A. Skatova. Moscow State Medical Stomatological University, Department of Children Therapeutic stomatology, Moscow, Russia. Aim: This was to compare assessment of enamels and dentine’s surfaces of primary teeth, prepared with different shape burs. Method: It was studying a sections of hard tissues like enamel and dentine’s surfaces with scanning electronic microscope after the preparation with different types of burs: tungsten carbide with 6 cutting sides; diamond dental drill with ISO 534 – green stripe; diamond dental drill with ISO 524 –red stripe. Results: The enamel of primary tooth had a microscope and splits after the preparation with hard-alloy burs. This outcome we revealed only with the usage of hard-alloy dental drills. Scanning microscope helps to see uneven, arraigner, rough dentine. This rind of burs cut the dentine, formed a big notches. In the contrary, after the preparation with diamond dental drill with green-mark, the enamel surface displays the smooth edge with out cracks and notches in the opposite the dentine’s structure has rough shape with little notches and sulkies. The dentine structure is the same as the one the hard-alloy burs preparation. After diamond drill preparation with red-mark, the enamel surface of primary tooth appears to be smooth, even, uniform, without splits and cracks. The dentine’s structure is smooth, even with little notches. They are less, then after the preparation with green-mark. Conclusion: The primary teeth preparation with us age of diamond bur with green-mark is the universe and optimal method. PND 14. Electric voltages and currents between amalgam and stainless steel tongue jewellery in vitro and in vivo. T. Tomaževič1*, D. Miklavčič2, G. Pucihar2,et al. 1Faculty of Medi- cine, 2Faculty of Electrical Engineering, University of Ljubljana, Slovenia. Aim: This was to evaluate the magnitude of electric voltages and currents between amalgam and stainless steel tongue jewellery (SSTJ) used for piercing. Method: The ball of the SSTJ was sunk into artificial saliva together with the ball of the same diameter made of five different amalgam brands. Electric voltages and currents between the pairs were measured. Similar measurements were performed in ten subjects with tongue piercing and amalgam filling on lower first molar. Results: Except for one amalgam brand, the voltages between amalgam and SSTJ, measured in vitro, decreased with time from –189.00 mV (SD 108.07) at the beginning of the measurement to –161.60 mV (SD 115.67) one hour later. The currents decreased with time as well, from –6.38 μA (SD 4.31) at the beginning of the measurement to –0.56 μA (SD 0.72) and –0.43μA (SD 0.51) after one minute and ten minutes, respectively. In vivo, the mean voltage of –37.5 mV (SD 66.05) was measured. The mean current was –1.33 μA (SD 6.05) at the beginning of the measurement and –0.08 μA (SD 0.07) after one minute. Conclusion: The existence of electric voltages between amalgam and SSTJ was demonstrated in vitro and in vivo. In vitro measurements showed their decrease with time. After Abstracts of Papers the contact between amalgam and SSTJ, there was also a measurable current detected, which decreased rapidly. The voltages and currents were smaller in vivo than in vitro, probably due to the presence of a pellicle on the fillings and pasivisation of the amalgam surface PND15. Microleakage of class V resin composite restorations after conventional and Er YAG Laser preparation in primary teeth. A. Vanden-Abbeele*, R. Atash. Dept. Operative (paediatric) Dentistry, Université Libre de Bruxelles, Belgium. Aim: To compare the mircoleakage of class V resin composite restorations following Er YAG Laser or conventional preparation. Method: 60 class V cavities with conventional bevel produced on the buccal surfaces of primary bovine incisors, and randomly divided to four group (n=15) Group I: T adper Scotch bond SE, Group II: Futurabond DC, Group III: AdheSE One, Group IV: Clearfil SE Bond. 60 class V cavities with ER YAG Laser produced on the buccal surfaces of primary bovine incisor and randomly divided to four group (n=15), Group V: T Adper Scotch bond SE, Group VI: Futurabond DC, Group VII: AdheSE One, Group VIII: Clearfil SE Bond. All cavities were restored with Filtek supreme plus. After thermocycling and immersion in 2 % methyl bleu the dye penetration were evaluated under a microscope. All date were analyzed by Kruskall-wallis test and Dunn’s multiple comparison. Results: The best seal was obtained by group V (adper Scotch bond SE + Laser Er YAG). Significant difference were recorded in the microleakage degree between the 2 types of cavity preparation (P< 0.05). Conclusion: Er YAG Laser treatment is recommended for composite restoration in class V cavities in primary teeth. PND16. Effect of Er YAG Laser on microleakage of contemporary adhesive to enamel and to dentine. F. Bashirtash*1, A. Vande abbeele 2, R. Atash1,2, 1Centre Dentaire Montjoie, 2Dept.(Paediatric Dentistry, ULB, Belgium. Aim: To investigate the microleakage in class V cavities following Er YAG Laser or conventional preparation restored with new bonding adhesive systems. Method: 60 class V cavities were assigned to 4 groups: Group I : T Adper Scotch bond SE, Group II: Futurabond, Group III: AdheSE One, Group IV: Clearfil SE bond. All cavities were restored with composite Fillek supreme after thermocycling and immersion in 2 % Methyl bleu, the dye penetration were evaluated under a microscope. Results: Statistically significant differences between enamel and cementum margin were observed (P < 0.05). In enamel and in cementum the best seals were obtained with T Adper Scotch bond SE to followed by clearfil SE bond, Futurabond and AdheSE One. Conclusion: Among the different adhesive systems used in this study, T Adper Scotch bond SE provide the best seal in enamel and the cementum margins. PND 17. Technique of detecting and reducing of dental phobia. A.V. Sevbitov, I. M. Makeeva, A. S. Bryzgalov et al. Moscow Medical Sechenov Academy, Moscow, Russia. Aim: To identify those factors which influence a patient’s psychological condition at the time of the dental appointment and to work out a system of measures to correct dental anxiety. Method: We examined 103 people 45 of which were under 18 years old and 68 of them were of age from 14 to 17 years old. All of those patients had clear signs of dental anxiety. Our investigation was carried out in the FGU MNII Clinic of Pediatrics and Children’s Surgery of the Russian Ministry of Public Health. Questioning was performed using special scales. During this investigation each time we carried out: common inspection, measuring pulse rate and blood pressure, determination psychological state of patients. Also we observed patient’s reaction to dentist’s actions at the time of dental appointment. Results: Almost 86% of patients of age from 14 to 16 years old and 75% of patients older 18 years old have clinically important stomatological phobia. In younger age group (14-17 years old) 12% of patients and in elder age group (older 18 years old) 25% of patients used pharmacotherapy Not only psychosomatics but also questioning using special technique is useful for detection of patient’s anxiety level. Conclusion: Psychological treatment is more effective in younger age group. Patients with stomatophobia in elder age group need pharmacotherapy more than a younger age group. PND 18. CASE REPORT. Treatment with local analgesia of a child previously treated under GA. C. Cuadros*, A. Lorente, F. Guinot, L. Bellet. Dept Paediatric Dentistry, School of Dentistry, Universitat Intern. de Catalunya, Barcelona, Spain. Background: General anesthesia (GA) is the elected treatment if the patient requires immediate, comprehensive dental care. The decision to perform dental treatment under GA is based upon age, ability to cooperate in a normal setting, medical status, and extent of treatment required. Many patients who undergo GA for dental treatment do not follow-up with 6-month recall exams, this may be associated with the outcome of GA. Case report: a male of 5 years old who was treated under GA because of Early Childhood Caries (ECC) one year and a half ago. He was referred to Universitat Internacional de Catalunya children’s clinic because of recurrent caries. Intraoral examination and radiographs showed absence of 54 and 64. There was occlusal caries in 55, 53, 51, 61, 65, 83 and 85. Teeth 34 and 44 showed affectation because of infection in teeth 74 and 84. Treatment: all restorative and surgical procedures were done under local analgesia. All decayed teeth 55, 53, 51, 61 and 83 were restored with composite. Pulpectomy of tooth 65 was done because of necrosis. Pulpotomy of 85 with MTA was performed. Teeth 55, 65, 75 and 85 were restored with preformed metal crowns. Teeth 74 and 84 were extracted previous antibiotic prophylaxis. A Nance appliance and a lower lingual arch were placed in order to maintain space. Follow-up: 6 months after the initial examination the patient remains in good oral condition. This case illustrates the need for periodic controls after treatment under GA and making the parents aware of the importance of a good oral hygiene after dental treatment. PND 19. CASE REPORT. Exposure and bonding of a maxillary impacted canine of an adolescent under IV sedation. L. Matharu*. Dental Institute Paediatric Dentistry Department, King’s College Hospital, London, UK. Background: Maxillary canine impaction has an incidence of 1 in 100 in the general population, ranging between 1% and 3% of patients. A palatally impacted canine is commonly exposed and bonded as part of orthodontic treatment, frequently under general anaesthesia. A report from the Standing Committee on Sedation for Dentistry 2007 states that intravenous sedation (IV) using a single agent may be an alternative technique in conscious sedation for children. Recognised and acceptable standards should be followed by a trained professional. Currently there are not enough IV sedation studies in children and more evidence based trials are needed. Case report: A 14 year old boy, ASA II was referred to The Paediatric Dental Department for exposure and bonding of the palatally placed 23. On examination patient seemed cooperative, however he was anxious regarding this surgical procedure, as he never had any previous dental treatment. Parents opposed to their son having a general an57 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 Abstracts of Papers aesthesia. Treatment: Following standard pre-sedation procedures, a cannula was inserted on the right anterior cubital fossa and 6 mg of midazolam titrated to achieve adequate sedation. Surgical exposure and bonding of the 23 was carried out and patient was taken to recovery and later discharged home with no adverse or side effects reported. Follow-up: The 23 was successfully positioned as part of the orthodontic treatment. Patient has no recollection of the surgical procedure and is now a candidate for routine dental treatment requiring no longer conscious sedation. PND 20. Behaviour management by the in vivo imitation technique in infant patients with small capacity for co-operation. V. B. T. Antunes*, F. G. Jimeno, L. J. B. Dalmau. Universitat Intern. de Catalunya, Spain. Aim: This was to evaluate if there is an upgrade on the behaviour and reduction on anxiety in children with small capacity for co-operation during conventional dental treatment after the In Vivo Imitation Technique application. Method: 20 children between 4 and 9 years old. A control group was taken with children with small capacity for co-operation in dental situations that needed a visit for restoration treatment, and an experimental group comprehended by children with small capacity for co-operation before the circumstances and/ or dental environment that require at least 2 visits for restoration treatment. The patient type was deter-mined by data registered on the clinical history as repeated experiences of disrupted behaviour and/or have shown dental anxiety problems to identify the no cooperation group. As for the children who assisted to the Magister in Integral Odonto-paediathry of the International University of Catalunya for the first time, a routinely clinical history was performed having special attention in the child’s attitude during the intrabucal exploration. Some variables were taken and compared: child’s anxiety autoevaluated before and after the treatment and the application of the In Vivo Imitation Technique, and the patient’s global behaviour during every visit. Results: Some statistically significant differences were found concerning the child’s global behavior perceived by the dentist and the anxiety autoevaluated by the child before and after the application of the In Vivo Imitation Technique. Conclusion: The In Vivo Imitation Technique as a learning method for proper behaviour and modification of the inadequate conduct in the dental environment proves to be valid in order to achieve co-operation in children with dental anxiety. PND 21. Effectivness of premedication agents admin-istered prior to nitrous oxide/oxygen sedation. Ö. Baygin1*, H. Bodur1, B. Isik2. 1s. Paediatric Dentistry Dept., 2Oral & Maxillo Facial Surgery, Gazi Univ., Fac. Dentisty, Ankara, Turkey. Aim: To evaluate the effectiveness of oral premedication with different agents on children scheduled for dental treatment under nitrous oxide/oxygen(N2O/O2) sedation. Method: After ethic committee’s approval and parental informed consent, 60 healthy children aged between 5-8 years, having no sedation or GA experience and incompliant with dental treatment (Frankl Behavior Scale≥3) were enrolled to this study. Children were randomly assigned to 4 groups. The treatment regimes were as follows: oral administration of 1mgr/ kg-1 Hydroxizine hydrochloride suspension 1 hour preoperatively (GroupI, n=15), oral administration of 0.7 mg.kg-1 Midazolam HCl 15 minutes preoperatively (GroupII,n=15), oral administration of 3mg.kg-1 ketamine+0.25 mg.kg-1 Midazolam HCl 15 minutes preoperatively (GroupIII,n=15) and no oral premedication was administered to the control group (GroupIV, n=15). Following premedication, 40%N2O and 60%O2 were administered to all groups. Sedation depth was evaluated using Ramsay Sedation Scale (RSS) 58 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 and data were recorded at 5-minute intervals. Data were statistically analyzed with ANOVA, Kruskal Wallis, Q-square and Friedman tests(p<0.05). Results: Achievement of sedation in terms of satisfaction/moderate satisfaction/unsatisfaction was as follows respectively: 13.3%/33.3%/54% in GroupI, 54%/20%/26% in GroupII, 33.3%/33.3%/33.3% in GroupIII and 6.7%/60%/33.3% in GroupIV. Conclusion: 0.7 mg.kg-1 midazolam was more effective than the other groups in children scheduled for dental treatments. PND 22. Amelogenesis Imperfecta- the earliest hereditary dental syndrome described and a case report with 20 years follow-up. U. Zilberman*, E. Mass, Section of paed. dentistry, Barzilai Medical Center, Ashkelon, Israel. Background: Amelogenesis Imperfecta (AI) was described in the Gar-ba IV child found at Melka Kunture, Ethiopia. It has been dated to circa 1.5 million years ago assigned to Homo erectus. The specimen comprises part of the right side of the mandible of a young child with a much worn first primary molar (dm1), errupted and unworn second primary molar (dm2) and exposed first permanent molar (M1). Visual examination indicated that all the enamel was abnormal. The peculiar appearance of the teeth, the extensive wear of dm1, the wrinkled occlusal surfaces of dm2 and M1 and the radiographic appearance resemble a known dental pathogenesis observed in modern populations, AI (MIM #301200) that represents a broad spectrum of genetic diseases affecting enamel formation in both primary and permanent dentition. The enamel abnormalities have been categorized into three major groups, hypocalcified, hypomaturation and hypoplastic. Case report: AN, a 15 years old girl was diagnosed as suffering from recessive hypoplastic AI that affected all her permanent teeth. Treatment: The treatment included coverage of her molar teeth with preformed metal crown (PMC) and composite coverage of all anterior and premolars. The final restorative treatment, at the age of 18, included 20 metal-fused ceramic crowns for all anterior, canines and pre-molar teeth. Follow-up: During the follow-up period of 17 years, no additional treatments were required except periodic plaque control. This presentation shows the antiquity of dental hereditary disorders and the need for aggressive prosthetic treatment in modern cases of hypoplastic AI. PND 23. CASE REPORT. Atypical dentine dysplasia in a 6-year-old boy. S. Ghadimi2*, H. Afshar1R. Ahmadi2. Dept Paediatric Dentistry, 1 Tehran University of Medical Sciences, 2Hamedan University of Medical Sciences, Hamedan, Iran. Background: Dentine dysplasia type I is a rare dental anomaly which is clinically characterized by almost normal looking crowns, short roots and mobile teeth. Case report: A case of an atypical dentine dysplasia type I affecting only first and second primary molars. Radiographic examinations of the teeth showed considerable short roots without pulp chamber obliteration. Apart from carious teeth with pulpal and periapical involvement, the apical area of the roots, PDL and supporting bone had normal radiographic appearance and there was no periapical pathosis. Histological analysis revealed dentinal disorganization in root dentine and multiple pulp stones in pulp chamber and root canals. The medical, dental or family history was unremarkable. Treatment: The teeth with periapical lesions were extracted and the remaining carious teeth were restored with PMC. To prevent pulpal and periapical infections, meticulous oral hygiene and effective caries-preventive instructions were given to the parents. Follow-up: After 2 years of follow-up except one premature exfoliated tooth, the others remained without any problems. Abstracts of Papers PND 24. CASE REPORT. Different Clinical Manifestations of Talon Cusp in the Primary and Permanent Dentitions. B. Celik*, E. Canoglu, Z. Cehreli. Dept Paediatric Dentistry, Faculty of Dentistry, Hacettepe University, Turkey. Background: Talon cusp is an uncommon dental anomaly that manifests as an accessory cusp-like structure, projecting from the lingual or facial surfaces of anterior teeth Cases Report: Case I: A 7-year-old boy was referred to the paediatric dentistry clinic for the treatment of anterior crowding. Talon cusps were observed on the buccal surfaces of permanent maxillary central incisors. Case II: A 4.5-year-old boy presented with a chief complaint of morphological anomaly of his maxillary anterior teeth. The patient had talon cusps on both buccal and palatal surfaces of primary maxillary central incisors. Case III: A 12-year-old girl was referred to our clinic for the management of aesthetic problems associated with her maxillary anterior teeth. Talon cusps were observed on both the buccal and palatal surfaces of permanent maxillary lateral incisors. Radiographic examination showed dens in vaginatus in permanent maxillary left lateral incisor. Treatment: No treatment was performed in Cases I and II, due to lack of occlusal and aesthetic concerns. In Case III, the caries-prone fissures of the talon cusps were restored with composite resin. Follow-up: All patients have been recalled uneventfully every six months, for a total of 1.5 years. PND 25. CASE REPORT. Concomitant hypohyperdontia and nonsyndromic oligodontia. S. Bektaş, M. Tekçiçek*, M. D. Turgut. Dept Paediatric Dentistry, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. Background: Concomitant hypohyperdontia is a very rare anomaly and described as the presence of both hypodontia and supernumerary teeth in the same region. Non-syndromic oligodontia is described as the congenital absence of more than six permanent teeth excluding the third molars. In the present case, concomitant hypo/hyperdontia and non-syndromic oligodontia in the same patient were presented. Case report: A 10 year old girl was referred to the paediatric dentistry dept. because of absence of her teeth and multiple spaces between them. Examination of the patient revealed a concave profile, protrusion of her mandibular, missing teeth, and anterior crossbite. In the radiographic examination, the absence of 12 permanent teeth both in the upper and lower jaws was noticed. Although there was an absence of the germs of the mandibular second premolars, bilateral tooth germs were detected near the distal root region of the mandibular primary first molars. Treatment: After the consultation with the dept. of genetics, it was learned that the patient had no genetic disturbance. Then, the primary second molars were extracted to facilitate the eruption of the ectopically located tooth germs. A removable partial denture for the lower jaw was inserted on the patient for functional purposes. Follow-up: Regarding the orthodontic treatment, it was decided to wait for the eruption of the permanent teeth. After three and a half months, the denture was found to be functional. PND 26. CASE REPORT. Supernumerary teeth in a twin brother with incomplete cleft lip. S. Bektaş*, M.D. Turgut, M. Tekçiçek. Dept Paediatric Dentistry, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. Background: Cleft lip and palate is a common congenital deformity encountered in the dental practice. Common dental anomalies located in the cleft region have been reported to be hypodontia, supernumerary tooth and tooth malformation. Similar dental anomalies have been reported in monozygotic twins, although a pair of dizy- gotic twin does not usually have the same anomaly. In the present case, supernumerary teeth were presented in a twin brother with an incomplete cleft lip. Case report: 7 years old dizygotic twin brothers were referred to the paediatric dentistry dept.. An unilateral incomplete cleft lip at the right side of one brother and the same type of the cleft at the left side of the other brother was detected. The clefts were noticed to be located on the lip region along with the philtrum line. Clinical and radiographical examinations revealed that the brother with the left side cleft had a supernumerary primary lateral incisor and a supernumerary permanent lateral incisor in the cleft lip region. Treatment: Carious teeth of the twins were restored and topical fluoride application was carried out in every three months. Follow-up: It was decided to follow-up the patient until the eruption of the maxillary permanent lateral incisors since the primary maxillary left lateral incisor did not cause a functional problem. This case demonstrated that patients with clefts should be inspected carefully in order to determine dental anomalies. PND 27. CASE REPORT. Cleidocranial Dysplasia and Failure to Erupt. E. Norton*, A. C. O’Connell. Division of Public and Child Dental Health, Dublin Dental School and Hospital, Ireland. Background: Cleidocranial Dysplasia (CCD) is a rare autosomal dominant condition that causes a generalised skeletal dysplasia; it is caused by a mutation in the gene encoding transcription factor CBFA1 (RUNX2). The classic feature is partial or complete absence of one or both clavicles. Intra-oral features include delayed exfoliation or resorption of the primary teeth, delayed eruption of permanent teeth, failure to erupt and multiple supernumeraries. Cases Report: Two similar cases of CCD are illustrated, focusing in particular on the failure to erupt of permanent teeth. Both cases were referred from their orthodontist to the Paediatric department of the Dublin Dental Hospital for assessment. Intraoral examination revealed a delayed dental development, with retained primary teeth, and radiographically multiple supernumeraries were visible. In both instances, the removal of the primary incisors and the relevant supernumeraries failed to result in eruption of the permanent incisors. Treatment: These cases require planned interdisciplinary care to allow timely and optimal eruption of the permanent teeth. In both these cases, the lack of eruptive potential of the incisors necessitated the use of orthodontic traction, complicated by the relative lack of anchorage. Alternative methods of exposure, traction and anchorage in the literature will be reviewed. Follow-up: Both patients are currently in active orthodontic treatment and will be reviewed regularly to time future intervention in posterior segments. PND28. CASE REPORT. Taurodontism and Klinefelter Syndrome. Dental anomalies as a hint for the diagnosis of the genetic disorder. R. Steffen*, H. van Waes. Klinik für Kieferorthopädie und Kinderzahnmedizin, Zürich, Schweiz. Background: Taurodontism is characterized by teeth with elongated pulp chambers and apical displacement of the furcation of the roots. Klinefelter Syndrom (KS) is a genetic disorder with genotype 47, XXY. Diagnosis of KS is made most often late in grown up age. The prevalence rates of taurodontism in Klinefelter syndrom patients is higher than in unaffected population. Learning disabilities and conspicuous social behaviour is also statistically related with Klinefelter syndrome. Cases report: Four patients presented with taurodontic teeth diagnosed during examination in our clinics. In every case diagnosis of taurodontism was made by x-ray. Social history showed in every case a conspicuous history (learning disabilities and school problems). Dentists recommendations enabled to ensure the diagnosis of KS. Treatment: In the presented cases, 59 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 Abstracts of Papers the finding of taurodont dentition first obtained no dental therapy, but led to further investigatory tests. The dentist’s information enabled the cooperating medical physicians the search with a direct aim. After that, in our shown cases the disorder KS was recognized. Conclusion: Male patients underlying health condition of taurodontism and learning disabilities have positive predictive value for KS. In case these findings are done during dental examination, the dentist should recommend karyotyping analysis to the patient or his parents. The paediatric dental practitioner should be familiar with taurodontism and related illnesses, especially KS, to help ensure the diagnosis of this chromosome constitution, which otherwise would remain unnoticed or in case of late diagnosis, would cost signify a loss of time in therapy. PND 29. Anomalies of tooth number and tooth size patterns. A. H. Brook*, R. C. Griffin, R. N. Smith. Int. Collabo-ating Centre in Oro-facial Genetics and Development, University of Liverpool, School of Dentistry, Liverpool. UK. Aim: To examine links between supernumerary teeth, hypodontia and crown size, determining the effect on the development of the whole dentition and so increase understanding of the aetiology of the conditions. Method: Measurements of mesiodistal and buccolingual dimensions were made using Mitutoyo electronic callipers of all teeth on study models. Mean value of 2 measurements was used and intra-operator and inter-operator reproducibility determined. The patients were young adults; 39 males and 21 females with supernumerary teeth and 60 age and sex matched controls. Results: Patients with supernumerary teeth had statistically significantly larger teeth (p<0.05) than controls, with greatest differences in the MD dimensions. In supernumerary and hypodontia patients the differences in tooth size were generalised throughout the dentition. Patients with hypodontia had smaller teeth than controls and this difference was statistically significant (p<0.05) for all teeth except the MD dimensions of some canines and premolars. Difference in size was greatest for the BL dimensions in hypodontia patients. The greater the number of missing teeth the smaller the tooth size. The hypodontia patients also showed higher variability in tooth dimensions than the control group. Conclusion: Genetic, epigenetic and environmental factors involved a complex aetiology of anomalies of tooth number also influence the size of teeth. Both supernumerary and hypodontia teeth involve the development of the whole dentition. These findings are compatible with a multifactorial aetiology of the conditions. PND 30. Dental caries in children with Down’s syndrome M. Kelmendi*1, F. Shllaku2, 1University Children’s Hospital and 2Dentistry Faculty, Prishtina, Republic of Kosova. Aim: To present the current prevalence of dental caries in children, as well as relationship between caries and predispose factors, such as : diet, oral hygiene practice, and use of fluoride supplements. 2. to compare dental status in children with DS and other population. Method: Information of dental health status, dietary intake etc. was gathered from a total of 50 children, 9-15 yr, selected at random from Elementary School in Prishtina , and 50 children with DS, aged 9-15 yr from Down’s Syndrome Kosova Center. Dental status was checked by dentist, in co-operation with paediatrician, using standardized protocol. Results: Of 50 children from the first group, 44 (88%) manifested dental caries, while 36(73.6%) children with DS have also dental caries. Questionnaire revealed also similar results for both groups, according to teeth brushing habit, regular dentist control, food habits, use of fluoride supplements etc. Conclusion: Although children with DS are protected from dental caries, our re60 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 sults show no difference between two groups, that means environmental conditions played a crucial role in the apparence of dental caries in children with DS. PND 31. The Neonatal Line - in an ultra structural view N. Sabel*1, J. G. Norén1, S. Nietzsche2. 1Gothenburg University, Sweden, 2Jena University, Germany, Aim: At birth, all primary teeth are in progress of mineralization. An incremental line in the enamel is seen which corresponds to the time of birth, termed; The Neonatal Line (NNL). The aim was to analyze the ultra structure of the NNL. Method: Manidbular right teeth were analyzed. Teeth were stored in formalin until processed into ground sections, bucco-lingual direction. Sections were etched and sputtercoated with either carbon or gold by vapor deposition. 3-D images were made in Philips SEM 515, ultra structural analysis was carried out in a field emission SEM (Gemini IMB, LEO 1530, Germany). Results: In the SEM, the NNL was observable in lower magnifications (40x), however, becoming more difficult to distinguish in higher magnifications. There was a change in diameter between the enamel prisms in pre- and postnatal enamel. A change in growth direction of the prisms was found in the region of the NNL. In the 3-D images at 400 times magnification the NNL was observable and visible as an indentation in the etched enamel, and not in non-etched enamel. Conclusions: The NNL is visible due to a change of direction of growth and a change of the diameter of the prisms. Indentation of NNL is an optical phenomenon due to the etching effect. PND 32. Enamel defects in children born in highrisk conditions: a preliminary study. E. Paiva*, R. Martín, L. Ferreira, J. et al. Dept. Paediatric Dentistry, University Complutense Madrid, Spain. Aim: This was to study the prevalence and distribution of enamel developmental defects (DDE) in children born in high-risk conditions and to investigate the correlation between the neonatal variables and the prevalence of enamel defects. Method: The study group consists of 60 children aged 4 to 8 years (Granada Hospital -Paediatric Department database) born in high-risk conditions (<2500g; need of neonatal intensive care). Control group consists of 60 children aged 6 to 8 years born in normal conditions. Teeth were examined clinically for DD, and were recorded using modified DDE Index. Results: Low birth weight/prematurely born children had a greater prevalence of DDE in primary dentition. Demarcated opacities were seen as the major defect in the study group. Conclusion: Neonatal data is important and has to be taken into account because it supplies information about oral sequels in the paediatric children born in high-risk conditions. Paediatric dentists should be able to establish preventive and therapeutic protocols for the children born in special conditions. Study support by Fondo de Investigación Sanitária (FIS) PND 33. Regularity of formation osseous face’s structures in postnatal period of developing. V. V. Stepanenko*, V. G. Smirnov.Dept. children’s Therapeutic dentistry, Moscow State Medical-Dental University, Moscow, Russia. Aim: This was to determine particularities in osseous texture of structures facial section of the head and possibilities of their use in dental practice. Method: 134 children, of different ages, were involved, using and integrated method which included classical, morph metrical (102), radiological and modern computer-based (32). Results: Research revealed, that facial bones have some particularities of age, mainly which can be determined by sequence of postnatal face’s organs developing. In comparing with early formation of eyesight as a half of brain, reveals in texture, connecting with its osseous struc- Abstracts of Papers tures. A part of the top dental plate, molar and palatine develops as a neural type, and the rest face’s bones develop as a somatic type. Less than 1 year’s old size of palatine outgrowth of the top dental plate is on average 21.7 mm, height of its body is 7.1 mm. Reverse, in the age of 16-18 years old, length of outgrowth increasing by 31.6 mm, height of dental plate is up to 41.8 mm. In accordance with dynamics of facial skeleton changing can determine physiological, postnatal formation of the organs (teeth, its rudiments). Conclusions: Consideration is due while surgical operation in organs facial section of the head, especially in cases when information is necessary, not only at the moment of the operation, but further on. PND 34. Study of dental anatomic peculiarities in juveniles and young adults based on computer tomography data. V. G. Alpatova* , L. P. Kiselnikova. Dept. Children’s Dentistry of MSMDU, Moscow, Dept. Children’s Dentistry of SPInStom, Saint-Petersburg, Russia. Aim: The aim of this study was to assess the anatomical peculiarities of teeth in juveniles and young adults. Method: 159 patient tomograms (aged 12 to 24) were analyzed as main group and 36 tomograms of patients aged 25-30 years as a control group. 83 maxilla tomograms of main and 18 of control group were investigated and 76 mandible tomograms of main and 18 of control group, accordingly. All tomograms were divided into group I: included incisors and canines (28 main and 9 control tomograms) and group II: included premolars and molars (131 main and 9 control tomograms). Attention was paid on size and structure of pulp chamber; width of root canal lumen; width of apical foramen and degree of its development; state of periodontal fissure (width, uniformity). Results: The width of apical foramen in juveniles and young adults was within the limits of 0.62mm to 0.80mm±0.01 (p<0.05) and width of periodontal fissure made 1mm to 1.2mm±0.005 (p<0.05), which exceeded the analog indices of control group with 0.52 to 0.66mm±0.009 (p<0.05) and 0.7 to 0.8mm± 0.006 (p<0.05), accordingly. Conclusion: Experience of using the dental computer tomography to explore the anatomical peculiarities testifies that this method is highly comprehensive and that during some years after eruption and completion of apexogenesis some anatomical peculiarities are kept in permanent teeth of young adults. Therefore while endodontic treatment of permanent teeth in young adults these peculiarities must be taken into account in endodontic techniques, allowing for higher treatment efficiency and lower incidence of possible complications. PND35. CASE REPORT. Anterior Crossbite. E. Ortiz*, F. Guinot, P. Martínez, L. Bellet. Universitat Internacional de Catalunya. Dentistry School Pediatric Dept., Spain. Background: Anterior crossbite refers to an abnormal lip-lingual relation between one or more incisive teeth of the maxilla and the mandible. It must be treated at an early age, to enhance dentofacial growth and development. Case report: One case of anterior crossbite in mixed dentition is presented in a patient who was 8 years and 1 month old at diagnosis. Treatment: After the diagnosis we decided to place a maxillary removable plate with springs on 11 and 21. Follow-up: One month later we observed a correct anterior occlusion. We made controls 2 months later to avoid a possible recidiva of the treatment. After three years the anterior occlusion was corrected PND36. CASE REPORT. Loss of space for the eruption of second lower left premolar. D. C. González*, S. Broch, L. J. Bellet. Dept Paediatric Dentistry, School of Dentistry, University International of Catalunya, Barcelona, Spain. Background: Premature exfoliation of the primary teeth results in the loss of space and the molar mesial drift, thereby altering the pattern of eruption of the first permanent teeth. In the last years several different new distalization devices were proposed and developed in order to minimize the patient’s complaints and optimize the biomechanical control. Molar distalization is a first therapeutic step in the treatment of Class II malocclusion without extractions. There are several systems to get the molar distalization which needs a minimal patient’s satisfaction. That`s why the “Molar Distalization” makes a patient feel comfortable and it does not require them co-operation and is absolutely aesthetic. Case report: Radiographic review of a female patient of 11 years old revealed loss of space between 36 and 34 by 36 mesialization, preventing eruption of 35. Treatment: In order to make space for the 35 a Molar Distalization device was used which was activated for the first time immediately after cementing. Then it was activated at intervals of 4 weeks. Follow-up: At first assessment eruption had begun. Two months later half of the clinical crown of 35 had erupted. PND37. CASE REPORT. Treatment of Class III Malocclusion by means of Function Regulator (FR-III). Y. R. Rhee*, Y. C. Choi, K. C. Kim, Dept Paediatric Dentistry, School of Dentistry, University of Kyunghee, Seoul, S. Korea. Background: Functional regulator III (FR-III) is used during the primary, mixed, and early permanent dentition to correct skeletal Class III malocclusion, characterized by maxillary skeletal retrusion. In mild or pseudo Class III children, this appliance can be the treatment of choice at the beginning. These cases are both Class III malocclusion with anterior crossbite that treated by FR-III appliances. Case report: 8 years old female and male attended Kyung-Hee University Dental Hospital to treat anterior crossbite; both were in mixed dentition and had Class III tendency and buccal crossbite. Treatment: Both cases were treated with FR-III. After 4 months of active treatment, the anterior crossbite corrected in one of the cases. In the other case, it took 3 months to correct anterior crossbite. In both cases FR-III was used for retention for 8 months and 20 months respectively. Space supervision was followed until the permanent dentition completed. No other appliance had been used in both cases. Follow-up: Four years after the correction of Class III, occlusions of the cases shows good stability. PND38. Mesio-distal Crown Diameters prediction of Canines and Premolars. A .Legovic*, A. Skrinjaric, D. Modrcin, et al. Private practice, Porec, Croatia. Aim: This was to determine the equations for accurate pre-diction of widths of crowns of unerupted canines (C) and premolars (P1 and P2) based upon the measured mesio-distal (MDD) and vestibulooral (VOD) diameter of crowns of erupted central (I1), lateral (I2) incisors and first permanent molars (M1). Method: Plaster casts from 180 subjects (90 boys and 90 girls) were examined. MDD and VOD of crowns of I1, I2, C, P1, P2 and M1 were measured twice on both sides in both jaws. Results: Regression equations for prediction of MDD of C, P1 and P2 crowns by using two ponders were defined as follows: Maxilla: girls: MDD 345=1.1711 x 32 or 42MDD+0.7028 x 36 or 46MDD +6.8197; boys MDD 345= 0.8073 x 12 or 22 MD + 1.0272 x 16 or 26 MD + 5.8167 Mandible: girls MDD 345= 0.8660 x 32 or 42 MD + 0.520 x 36 or 46 MD + 10.1248; boys MDD 345= 1.1311 x 16 or 26 MD + 1.4757 x 32 or 42 MD + 0.8473. Conclusion: Prediction accuracy of determined equations for mesio- distal dimater of crowns of C, P1 and P2 in both sexes and both jaws varied from 97.0 to 97. 61 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 Abstracts of Papers PND 39. CASE REPORT. Fixed functional Herbst appliance in the treatment of Class II(i) malocclusion V. Zivojinovic*. PND 41. Dependence of a level of dental diseases of pupils from a level of knowledge on questions of preventive dentistry. N. Nedeljkovic, Clinic of Orthodontics, Clinic of Preventive and Pediatric Dentistry, School of Dentistry, University of Belgrade, Serbia. I. A. Lysenkova, Y. L. Vasiliev, A. I. Rudnev. Dental Dept., Moscow State Uni. of Medicine and Dentistry, Russia. Background: Sagittal mandible deficiency is the most common cause of skeletal Class II malocclusion. Treatment objective was to stimulate sagittal mandible growth. Herbst fixed appliance use is beneficial for shortening the time required for treatment and does not depend on patient compliance. Case report: A female 13.5 years of age was referred to the Clinic of Orthodontics, School of Dentistry in Belgrade following previous unsuccessful treatment of her skeletal Class II malocclusion using an activator. The patient’s poor co-operation had led to failure of the treatment. Treatment: Patient was subjected to the Herbst treatment for 6 months followed by fixed appliance for another 8 months. Lateral cephalograms before and after the treatment were performed. Condyl and fossa articularis remodelation was assessed by superimposition of pre- and post-treatment TMJ tomograms. Promotion of oral hygiene and fluoride use was performed because orthodontic treatment carries a high caries risk and risk for periodontal disease. Skeletal and dental changes were observed after treatment (correction (Max+Mand): molar relation 6mm, overjet 7mm, skeletal 5mm, molars 1mm and incisors 2mm). Combination of Herbst and fixed appliance was effective in the treatment of dental and skeletal irregularities as well as in TMJ remodelation for a short period of time. Follow-up: In retention period, 10 months after the treatment, occlusal stability exists. Follow-up care in oral prevention is based on regular recalls at the dental office and supervision at home by the parents. Aim: This was to define the correlation between the dental status of teenagers and their knowledge of oral hygiene. Method: 100 teenagers, average age was 9-15 years were questioned on their knowledge of preventive mainten. The level of hygiene of an oral cavity, prevalence and intensity of the basic dental diseases, such as caries and periodontal diseases were also assessed. Teenagers were divided into two groups according their main source of information: advertising and the others. Results: 1. Questionnaire has shown that 37 % teenagers use advertising as the basic source of the information on means of oral cavity hygiene they get; 54 % teenagers get information mostly from parents and 9 % of them mention the dentist. 2. Prevalence of caries at children marking advertising as a source of the information on preventive maintenance was a little bit higher (93), than at children indicating family and the dentist (81). Intensity in both cases was the same: 4,66 ± 0,23. 3. The level of prevalence and intensity of periodontal diseases in 1st group was “low” while in 2nd group it was “average”. Conclusions: Oral hygiene and the condition of periodontal tissues and firm tissues of teeth is dependant on a level of pupils’ knowledge. PND 40. Association of Passive Smoking with Caries and Related Salivary Biomarkers in Young Children. A. Avşar*, Ö. Darka, B. Topaloğlu, et al. Dept Paed. Dent School of Dent, Dept Clin Microb. Dept Biostatistics School of Med, OMU, Samsun, Turkey. Aim: This was to investigate association of passive smoking as determined by salivary cotinine levels with caries and salivary pH, buffering capacity, flow rate, and microbiological counts in young children. Method: 90 passive smokers (PS) with a mean age of 5.02 years and 90 healthy age-matched children were included in this study. Family income, smoking habits, the education level of the parents, tooth brushing, and daily dietary sugar exposure of the children were recorded by questionnaire. Three categories were formed with respect to the number of cigarettes smoked. A dmft score was calculated according to the criteria recommended by the World Health Organization. The children were also investigated for stimulated salivary cotinine levels, pH, flow rate, buffering capacity, and salivary mutans streptococci and lactobacilli colonization. All data were analyzed using SPSS, version 13.0. Results: The dmft scores, salivary pH, flow rate, buffering capacity, mutans streptococci, and lactobacilli colonization in PS children were significantly higher than the healthy subjects (p<0.05). There were no significant differences in tooth brushing and dietary habits of the children, family income, and education levels of the parents between the two groups (P > 0.05). The mean cotinine levels of PS children was 1.58 ± 4.3 ng/mL. The dmft scores, buffering capacity, flow rate, mutans streptococci, and lactobacilli colonization were significantly different between each of the three categories (p<0.05). Conclusion: PS children were at an increased risk of caries in comparison with the healthy subjects. 62 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 PND 42. Effect of a New Enzyme Containing Toothpaste on Saliva SM and Buffering Capacity: A pilot study. A. Durhan*, B. Kargul. Marmara University, Dental School, Dept. of Pediatric Dentistry, Istanbul, Turkey. Aim: This was to evaluate the effect of First Teeth Baby Toothpaste (FTBT) on the counts of Streptococcus mutans and salivary buffering capacity. FTBT (Laclede, USA) contains Lactoperoxidase inhibitory enzyme system. These natural protective enzymes are found both in saliva and breast milk and are essential in reducing cariogenic bacteria. Method: Nine 1-3 years (2.4±0.58) old children with Early Childhood Caries (ECC) were included in this study. Saliva samples taken at baseline and after 1 month were assayed for .S. mutans and salivary buffering capacity. Salivary levels of SM were estimated by Dentocult SM (Orion Diagnostica Co. Ltd, Epsom, Finland). Salivary buffering capacity was evaluated using Dentobuff strips (Orion Diagnostica Co. Ltd, Epsom, Finland). Chidren were asked to use FTBT twice a day for 1 month. Results: Some significant changes were observed in salivary parameters after 1 month. The average S. mutans counts in the primary dentition of children with ECC before and after using FTBT were 105 colony forming units (CFU)/ml and 103 CFU/ml respectively. S. mutans were statistically significantly reduced relative to baseline at 1 month (p=0.017). No significant changes in saliva buffer capacity (p=0.104) after FTBT were observed. Conclusion: First Teeth Baby Toothpaste can be recommended as part of caries prevention programs targeting infants and toddlers. It is important to determine, through larger and more in depth clinical trials. PND 43. The Research of Nanostructures of Pigmented Dental Plaque in Children. V. D. Shchegoleva*, V. A. Shahnov, A. I. Vlasov, et al. MSTU, MSUMD, Russia. Aim: This was to receive theoretical footing and to verify a supposition of possibility to consider dense pigmented dental plaque to be a nanostructure. Method: The tooth structures were researched on longitudinal and transversal tooth slices by comparison the slices with pigmented dental plaque and without it from teeth removed Abstracts of Papers by medical indications. The atomic force microscope Solver P47HPRO and wide spatial range scanner SC150 (50х50х3 micron) were used. Semi-contact microscopy method was chosen. The microscope Intel was used for tooth slices macrographs. Results: Expert assessment of tooth slices was carried out through different magnifiers. Analysis of resulting tooth slices exhibited that the size of dental plaque structures was not less than 150 nanometers. These objects were about 0,8–2 microns on average, in other words their size was much bigger than nanotechnological limit of 100 nanometers. Conclusion: Pigmented dental plaque does not belong to nanostructured objects. PND 44. Study on mothers’ concern regarding their children’s oral health. I. Totolici*, C. Puscasu, D. Totolici, et al. Dept Paediatric Dentistry, Faculty of Dentistry, University Ovidius, Constanta, Romania. Aim: This was to study the relation between the mothers’ level of education and their concern regarding the treatment necessity and the prevention measures used for the children oral health. Method: 107 mothers who came in the Clinic of Paediatric Dentistry of the Faculty of Dental Medicine in Constanta, Romania during one semester period agreed to receive and fill a questionnaire. This contains 10 questions with two or three items. The questionnaire was designed in three major directions: the concern for dental treatment, the treatment needs and some knowledge regarding prevention, all related to the mothers’ years of school. The software SPSS for Windows, version 10.0 was used. Sampling error was 4.12 and the level of significance α=0,05. Results: The distribution was: group A (elementary school) 16.8%, group B (high school) 58,%, and group C (college) 25.2%. The study revealed (α = 0,05) that the mothers from group C bring earlier the children in the dental office, in comparison with group B and A. 96,30% of the mothers group C bring from the first time the children in the dental office for the reason of checking, in comparison with group A and B with 50,00%, respectively 45,16%. In chidren with mothers in group C the toothache frequency is lower (18.5%) than those from group A (61.1%) and group B (56.4%). The age when children begin for the first time toothbrushing is in majority 3 years of age for group B and C, but in the group A many children start toothbrushing at 6 even at 9 years of age. Conclusion: The mother’s level of education reflects in the oral health PND 45. Effect of fluoride varnish on development of artificial caries. S. H. Bak*, N. Y. Lee, S. H. Lee. Dept. Paediatric dentistry, Chosun University, Gwangju, South Korea. Aim: This was to evaluate the effect of fluoride (F) varnish on prevention of enamel demineralization. Method: 80 bovine enamel blocks were divided randomly into 4 groups of 20 specimens: Group 1, served as the control with no topical application of F. Group 2, treated with APF gel for 4 minutes. Group 3, treated with Fluor Protector®. Group 4, treated with CavityShield™. After 24 hours of treatment, samples were brushed with a soft bristled tooth brush to stimulate normal mechanical wear of varnish. Artificial caries lesion was induced by placing each specimen into demineralization solution at pH 4.0 for 48 hours. Then the optical density of the lesion was measured by light fluorescence induced by plasma light and the surface microhardness was measured by the vicker’s hardness test. Results: 1. The optical densities of group 3 and 4 were significantly higher than those of 2, but no significant difference was noted between group 3 and 4. 2. The surface microhardness of group 4 was the highest, followed by group 3, 2, and 1(p<0.05). 3. There were no significant difference between group 3 and 4 in optical density. However, the surface microhardness of group 4 was significantly higher than that of group 3. Conclusion: The results of present study in- dicate that the fluoride varnish is more effective than APF gel for prevention of dental caries. PND 46. The Effect of Syrup based Medicines for Children on Intraoral pH. S. Kim*, I. Y. Kim, G. L. Lee, et al. Dept Pediatric Dentistry, Pusan National University, Pusan, Korea. Aim: This was to investigate the acidity level of syrup form drugs frequently prescribed for infants and young children, and the effects on intraoral pH when rinsed with them. Method: 11 kinds of syrup form drugs prescribed by our hospital and 4 kinds of OTC syrup form drugs were selected and their pH were measured in vitro. 5 volunteers as examinee were told to wear customized mandibular vacuum-formed appliances incorporating pH electrode, rinse intraorally with 4 kinds of test drugs and the changes of plaque pH were measured every minute for 40 minutes. Also the salivary pH changes after Cough syrup rinsing with/without water rinsing were measured every 4 second for 5 minutes. The results were analyzed statistically with repeated measure ANOVA and Duncan test. Results: Average pH of 15 syrups form test drugs was pH 4.7±0.94, range of pH 3.0-6.8. After rinsing with 4 kinds of test drugs plaque pH decreased below pH 5.5,critical acidity for enamel demineralization. There was no significant difference in the curve of plaque pH profile between 4 kinds of drugs except COLDI (p>0.05). There was significant difference in salivary pH changes between rinsing syrup only and rinsing it followed by water (p<0.05). Conclusion: Special care is needed when selecting and using the syrup form drugs for children as these result in significant decrease of intraoral pH. PND 47 To compare the rate of demineralization using carbopol 907 system with carbopol 2050 system. M. Yoon.*, Kim Jong su, Yu Seung hoon. Dept Paediatric Dentistry, School of Dentistry, Uni. of Dankook. South Korea. Aim: The purpose of this experiment is to alternate the remineralizing solution of carbopol 907 system to new carbopol 2050 system Caries lesion produced by carbopol 907 system represents true natural caries lesion repeatedly. But these days, carbopol 907 system is not available in market. Method: The rate of demineralization was measured by DIFOTI (Didital Imaging Fiber-Optic Transillumination). The demineralized site was produced by carbopol 907 system and carbopol 2050 system. 42 primary teeth were prepared and divided by experimental schedule. First week, Images were taken of DIFOTI on four teeth each day from each group. After one week Images taking of DIFOTI had done every another day until 20th day. Results: The rate of demineralization of 907 system is faster than carbopol 2050 system. Conclusion: The rate of demineralization of carbopol 2050 system is slower than carbopol 907 system. PND 48. CASE REPORT. Sound teeth? Your choice! G. Stel*, J. C. Smienk1, J. J. Bruers1, 2, 1Dutch Dental Association (NMT), Nieuwegein, 2St Radboud University Medical Center, Nijmegen, Netherlands. Background: Well over 75% of the children in the Netherlands have good to very good oral health. In about one quarter of all cases this is less so, whereas in around 5% we even speak of serious oral health issues. Case report: In 2007 the Dutch Dental Association (NMT), the professional organisation of dentists, started with the program: Sound teeth? Your choice! This is a national project, which focuses on the development of coherent policies to improve oral health in youths in the Netherlands. The project should lead to a higher percentage of children with a sound dentition in all age groups and to adequate treatment of as many children as possible, preventively 63 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 Abstracts of Papers and curatively. Treatment: Sound teeth? Your choice! goes into various aspects of oral health in youths. The program aims at intensifying collective dental prevention to influence in a positive way knowledge of and attitude towards the oral health situation of (the parents of) children. Initiatives are taken to ensure that children will always regularly see a dentist from the age of two. Finally, the activities are geared towards optimising the professional dental treatment of youths. On the one hand through improvement of knowledge and skills within the profession, by means of developing a clinical guideline and task delegation protocols, on the other through setting up a nationwide referral network of paediatric dentists. Follow-up: During the program efforts will be made to achieve that the quality of oral health care is structurally guaranteed and permanent funding is available. PND 49. ‘I thought I might get bullied a bit’ The impact of oral conditions during educational transition. Z. Marshman*, J. Bradbury, M. Hall, et al. Dept. Oral Health and Development. School of Clinical Dentistry, University of Sheffield, UK. Aim: To explore experiences of young people with dental/oro-facial conditions as they undergo the transition to secondary education. Method: The theoretical framework chosen to guide the study was symbolic interactionism. A 2-week diary was developed in collaboration with young people. The diary was sent to 21 11- and 12-yearolds to complete around the time they started a new secondary school. The participants were then interviewed in their own homes about the content of the diary and their experiences during the educational transition. The interviews were audio-taped and transcribed verbatim. The data were analysed using the constant comparative method. Clinical data were obtained from the participants’ dental records. Results: 18 diaries were returned, but one young person was not interviewed for medical reasons. The clinical conditions included malocclusions, cleft lip and palate, treated incisal trauma and developmental defects of enamel. These had varying impact on the participants as they underwent the educational transition. The main themes to emerge were: ‘making friends’ and ‘fitting in’. No links between gender, severity of condition or experiences of secondary school were apparent. Conclusion: Transition to secondary education was a significant life event with oral conditions affecting young people to varying degrees. PND 50. CASE REPORT. Management of cervical root fracture using orthodontic extrusion & natural crown reattachment. T. I. Keceli*, H. C. Gungor. Dept Paediatric Dentistry, School of Dentistry, Un. Hacettepe, Ankara, Turkey. Background: Root fractures affect 0.5% to 7% of permanent teeth. Cervical root fractures, seen less frequently, have a worse prognosis compared with the fractures in the apical or middle third of the root. Case report: 10-year-old boy was brought to the paediatric dentistry clinic by her mother with the complaint of tooth fracture one day after dental trauma. Treatment: Because of the high mobility and the radiographic findings, the coronal fragment needed to be extracted. After removal of the coronal fragment, the root was filled temporarily with calcium hydroxide. Following the permanent root canal treatment, orthodontic extrusion was performed. The apical portion was elevated above the epithelial attachment and a successful coronal restoration was made using the natural crown of the traumatized tooth. Follow-up: After 6 months, the clinical and radiographic results were successful. 64 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 PND 51. CASE REPORT. Treatment of a Subluxated Central Incisor. S. Acar*, E. Sutekin, O. Koca, et al. Dept. Paedodontic Ege University, Turkey. Background: Subluxation is one of the most severe types of dental trauma in children. Case report: This report presents a case of subluxation of mature maxillary central incisor in a 14-year-old boy. Patient was referred to dental clinic at Ege University due to a severe dental trauma. The crown of central incisor was pushed palately. The root of the tooth was out, through the vestibular mucosa. Treatment: The subluxated tooth was immediately repositioned surgically within 5 hours after trauma. Under local analgesia the traumatised tooth was pushed back and repositioned in its alveolar socket and was splinted for four weeks. Chlorhexidine mouthwash and amoxicillin were prescribed. During the stabilization period, the pulp was removed and root canal was disinfected by calcium hydroxide at recalls. The final root canal treatment with guttapercha was completed after the removal of splint. During recalls intracoronal bleaching agent was applied to the tooth. Follow-up: There was no pathology and resorption found in 4 and 8 months examinations. The patient is being followed up. PND 52. CASE REPORT. Consequences of traumatic injuries in primary teeth on the permanent germ of the developing one. E. Díaz*, H. Boix, F. Guinot, et al. Dept. Paediatric Dentistry, School of Dentistry, Universitat Internacional de Catalunya, Spain. Background: The traumatic injuries in primary teeth, as the inadequate treatment of such, can have negative repercussion on the permanent germ of the developing teeth. There are multiple alterations that can take place, whose affection is going to depend on the intensity of the trauma and the stage of the development in which it takes place. The treatment of the immediate complications of a dental traumatic injury is usually limited to urgent measures, whereas the clinical revisions of any possible later complications of the development of the permanent dentition is habitually ignored. Case report: We presented a case report of a 7 years old female patient, with a coronal alteration of the 11, associated to a traumatic injury of the temporary predecessor at 3 years of age. Treatment: The first step was to expose the margins of the injury by eliminating gum with electrical scalpel. While cleaning the injury and eliminating the enamel without support, was made a pulpal exposure that was treated by covering pulp directly. Follow-up: We observed the evolution of the case in the 6 later months after the treatment. The case demonstrates the control importance of the possible later sequels of a traumatic injury in primary teeth on the permanent germ of the developing one. PND 53. Endodontic intervention in treatment of dental injuries and their complications in permanent teeth of children. V. Merglová*, V. Tzigkounakis. Charles University in Prague, Dentistry Department, Faculty of Medicine in Pilsen, Czech Republic. Aim: To determine the necessity of endodontic intervention after various dental traumas in permanent teeth with open apices and in teeth with closed apices. Method: The records in the documentations of 178 patients who were treated for dental traumas in the period 1995 – 2005 in Dentistry Department of Faculty of Medicine in Pilsen were analysed. Results: In 70 lateral luxations in teeth with open root apices, the most frequent posttraumatic complications, diagnosed within 24 months after the injury, were obliteration of root canal (19 cases, 27.1%) and pulp necrosis ( 6 cases, 8.6%). In 96 Abstracts of Papers lateral luxations in teeth with closed root apices, the most frequent complications were pulp necrosis (15 cases, 15.6%), resorption of the root (9 cases, 9.4%) and obliteration (8 cases, 8.3%). In 50 uncomplicated clinical crown fractures of teeth with open root apices, pulp necrosis (7 cases, 14%) was the most frequent posttraumatic complication, but in 51 fractures of teeth with completed root formation, the most frequent complication was periodontitis (8 cases, 15.7%). Finally, in 15 crown fractures with exposed pulp in teeth with open root apex, 6 were treated with pulp exstirpation (no posttraumatic complications were noticed), 6 with pulpotomy (3 out of them presented with periodontitis) and 3 with direct pulp capping (all 3 resulted in pulp necrosis). Conclusion: Dental traumas require frequent follow-ups. In that way dental practitioners can early diagnose posttraumatic complications, which usually require acute endodontic treatment. PND 54. CASE REPORT. Periodontal pocket as a consequence of traumatic dental extraction. C. Stiénon*, H. El Yazami, T. Vanhée, I. Loeb. CHU Saint-Pierre, Univ. Libre de Bruxelles (U.L.B.), 1000 Brussels, Belgium. Background: Occurrence of periodontal pocket with severe inflammation as a consequence of traumatic dental extraction. Case report: A 14 years-old boy presented a localized bone loss of 8 mm between right mandibular premolars (44 and 45) after an orthodontic treatment. Prior to the treatment, two temporary teeth (75 and 85) were removed. The patient complained of pain in the extraction socket site (85). Treatment: The periodontal pocket was debrided, treated with heterologous bone bank grafts and protected by a resorbable membrane (Surgicelâ). After one month, the periodontal exam shows 2 mm depth at this site. Follow-up: 2 and 6 months, 1 and 2 years after the initial therapy, the bone level has not changed. This case report emphasizes on the importance of a multi-disciplinary follow-up for children who are orthodontically treated. PND 55. WITHDRAWN PND 56. WITHDRAWN PND 57. A Prospective Study of Cleft Lip and/or Palate Incidence in BaRia Vung Tau, Vietnam. J. Lucas1*, J. Christie1, P. Lam Hai2, et al.1Melbourne Australia, 2 National Hospital, Ho Chi Minh City, Vietnam. Aim: The aim of this prospective study was to determine the incidence of cleft lip and/or palate in the province of Ba Ria Vung Tau, South Vietnam, whilst simultaneously demonstrating an interdisciplinary model of care. Method: One hundred and eighty health professionals from 2 hospitals, 8 health centres and 84 health stations in the province of Ba Ria Vung Tau were trained to record the number of infants born with/without cleft lip and/or palate over 12 months. Within 6 months of birth registration, the infants with CL and/or P were visited at home and genetic history, comorbidities and feeding methods were recorded. Results: Between June 2005 and May 2006 eleven infants with clefts were born in the southern province including 3 unilateral clefts of the lip alone, 4 unilateral cleft lip and palate, 1 palate only and 3 bilateral cleft lip and palate. The incidence of cleft lip and/or palate, provincial distribution, type of cleft and feeding methods, genetic history, medical history, the presence of co morbidities will be presented. Conclusion: The interdisciplinary model of care and the use of the data to assist in identifying the need for service development at both provincial and national levels will also be discussed. PND 58. WITHDRAWN PND 59. CASE REPORT. Marsupialization as Conser-vative Treatment of Odontogenic Keratocyst in a 12-year old child. Y. Gonzalez*, C .Palma, A. Cahuana, et al. Hospital Sant Joan de Déu, Barcelona, Spain. Background: Odontogenic Keratocyst (OKC) is a common cyst, locally aggressive and highly recurrent. It is frequently found in the retromolar area of the mandible. It is believed to arise from remnant cells of the dental lamina. Case report: A 12-year old female was referred to the Hospital due to intraoral secretion in the lower right posterior region. The patient had neither pain nor swelling. Clinically a small sinus tract was noted next to the lower right second permanent molar (47), which secreted spontaneously. There were deep probing pockets located distally to the molar. The panoramic X-ray and CT scans revealed a huge unilocular radiolucency with densely corticated margins extending from the distal area of the 47 to the ascending ramus, which enveloped a displaced third lower molar. Histologically, the lesion was consistent with OKC. Treatment: Initial therapy was focused on decompression of the lesion by means of a removable acrylic appliance with an attachment that allowed intracystic drainage. The following ortopantomograph 1 month later, showed a decrease in the size of the lesion. Under general anaesthesia the OKC was enucleated and the impacted third molar extracted. Follow-up: Evolution of the lesion is favourable 1 year post surgical treatment. However, the patient is under strict control due to the high probability of recurrence. Marsupialization with an appliance can relieve pressure within the cyst, so that subsequent enucleation can be performed more simply and conservatively. PND 60 CASE REPORT. Laser assisted treatment of a dentigerous cyst. C. Hahn*, J. R. Boj, C. Poirier, et al.Dept. Paediatric Dentistry, University of Barcelona, Spain. Background: Dentigerous cysts are benign maxillary odontogenic cysts associated with the crown of an unerupted tooth. They can expand the cortical bone to such an extent that they cause displacement of teeth and root resorption in the adjacent teeth. Case report: This is a clinical case of a dentigerous cyst in a mandibular permanent molar of a 6-year -old child. The cyst caused the displacement of the bud of the permanent second molar and root resorption of the primary second molar. Treatment: The cyst was treated by: (1) mucous fenestration using an erbium laser; (2) drainage of the fluid content; and (3) curettage of the bone cavity. Follow-up: The injury was successfully resolved in just 5 months by: (1) bone regeneration; (2) the repositioning of the displaced bud; and (3) correct eruption of the affected tooth. PND 61. WITHDRAWN PND 62. Condition dorsal surface of language and parodont at children with diseases of bodies of digestion. V. M. Elizarova*, A. V. Dikaya. The Moscow State University of Medicine and Dentistry, Faculty of children\’s therapeutic stomatology, Moscow, Russia. Aim: This was to study the condition of fabrics the parodont and dorsal surface of language at a various pathology of bodies of digestion. Method: 50 children, from 10 till 17 years, with the diagnosis were clinically surveyed for: ulcerative colitis, Crohn disease-13 (Gp A), chronic mucous colitis-19 (Gp B), chronic gastritis, gastroduo65 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008 Abstracts of Papers denitis-18 (Gp C). The group of comparison was made by 35 children without pathology (Gp D). Results: In all children it was revealed chronic marginal gingivitis. Index PMA (Parma C., 1960): Gp A-34, 82; Gp B-35, 63; Gp C-39, 40; Gp D - 22, 42. At all children were changes papillae the device of the language, shown hypertrophy filiform papillae, mainly in average and distal parts of language. The hypertrophy fungiform papillae have come to light on periphery of language and on its tip. The dense white, yellowish, greyish strike which is settling down mainly in a back and average parts of language - at 50. Longitudinal cracks and folds of language - at 22, puffiness of language, prints of a teeth - at 25, sites desquamation of epithelium on a back and lateral surfaces of language - at 8. The index of language strike (Yaegaki K., 1998) has made accordingly 2, 75 in Gp A; 2, 17 in Gp B; 2, 41 in Gp C; 1, 31 in Gp D. Index WTC (Winkel Tongue Coating, E. Winkel (1998)) has made 9, 9 in Gp A; 7, 6 in Gp B; 9, 3 in Gp C; 3, 8 in Gp D. Conclusion: Diseases of various departments of digestive system at all at children are brightly shown on a mucous membrane dorsal surface of language and in fabrics of parodont. Obtained data allow confirming the importance of methods of clinical diagnostics accessible to everyone doctor. PND 63. WITHDRAWN PND 64. WITHDRAWN PND 65. CASE REPORT. Conservative Clinical Management of Periodontal Disease in two Children. C. Palma* , Y .Gonzalez, A. Cahuana. Dept. Paediatric Dentistry Hospital Sant Joan de Déu and University of Barcelona, Spain. Background: Destructive forms of periodontal disease in children are uncommon. Generalized Aggressive Periodontitis (GAgP) can be a manifestation of a systemic disease and generally fails to respond to standard treatment. For this reason, therapeutic management remains controversial; some support conservative treatment, others radical. Cases report: A female of 4 years of age and a male of 3 years were referred to the Paediatric Dentistry Department at the Hospital Sant Joan de Déu of Barcelona due to early loss of incisors. Their medical histories were uneventful. Intra-orally they both had generalized gingival inflammation and bleeding upon probing, as well as moderate attachment loss. Moreover, severe mobility affected the remaining dentition. They were both caries free. Dental X-rays in both cases showed horizontal bone loss in the entire dentition. Blood analysis showed no association with systemic diseases. Treatment: Both children were treated with antibiotics and extractions of only severely affected teeth, keeping the remaining under frequent scaling and strict measures of oral hygiene. When gingival health was achieved, both cases were rehabilitated with prosthetic removable appliances to restore function and aesthetics. Followup: Three years after dental management both children’s periodontal health remains in good conditions that allowed the normal eruption of permanent teeth. These cases show that successful outcome can be achieved with early diagnose and conservative treatment. They are actually under strict supervision every 6 months. 66 European Archives of Paediatric Dentistry // Abstracts of EAPD Congress, 2008