Download Brenda Green-Jarvis

Brenda Green-Jarvis
442 Lynn Dr. Nashville, TN 37211 615/831-1892 [email protected]
LinkedIn: Brenda Jarvis ResearchGate: Brenda M. Jarvis
SUMMARY
Academic research scientist with extensive scientific experience and skilled in 3D spheroid cell culture,
immunofluorescence, confocal microscopy, Elisa, and Western blotting is seeking a research position.
OBJECTIVE
Highly organized, detail oriented, and broadly trained scientist with as well as a hands-on get-it-done attitude
seeking an opportunity for assay and product development and scientific achievement.
TECHNIQUES and SKILLS
Protein Biochemistry
Protein Purification
- dye-ligand chromatography
- ion exchange
chromatography
- Preparative Electrophoresis
- Solution Isoelectric Focusing
with Rotofer Cell
ELISA
Binding Kinetics ELISA
MMP Activity ELISA
Zymographs of MMP Activity
Western Blot Analysis
Molecular and Cellular Biology
Plasmid Subcloning
Site Directed Mutagenesis
Mammalian Cell Culture
In situ Hybridization
Immunofluorescence
In vitro migration assays
Expression of GFP and Luc in
Mammalian cells
Confocal Microscopy
FACS analysis
3D Spheroid Mammalian Cell
Culture
Animal Imaging
Bioluminescence
Fluorescence
Image Analysis
Animal Care
Manage colony and breeding
Necropsy
Harvesting Primary Tissue
Writing Animal Protocols to
Vanderbilt standards from grant
drafts
Computer Skills
Image Analysis
Microsoft Office
GraphPad Prism
ImageJ
Adobe Acrobat
Adobe Photoshop
ChemDraw
Endnote
Organizational Skills
Image Preparation
Basis Statistical Analysis
Creation of Data Analysis Templates
Set up electronic lab organization.
Organized lab materials, including
tissue sample storage and
computer files.
Ordered supplies and monitored lab
expenses
Set up and maintained a computer
imaging and densitometry station
for gel analysis and cell culture
documentation.
Supervised projects of summer
program students, medical
students, and graduate students.
Effective at trouble shooting
experiments and identifying
solutions in an academic research
lab
Drug Studies
Dose Response Analysis using
Incucyte for data acquisition
PROFESSIONAL EXPERIENCE
VANDERBILT, Nashville, TN
Research Assistant II (position eliminated, no grant funding)
Managed lab supplies and ordering.
Subcloning and DNA plasmid preparation
Learned new technique with drug dose response analysis using Incucyte Imaging system.
Helped to close out the lab.
2015-2016
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VANDERBILT, Nashville, TN
2009-2015
Research Assistant II (position eliminated, no grant funding)
Managed lab supplies, safety inspections, animal protocols.
Learn new techniques including FACS, Confocal Microscopy and 3D cell culture
• Worked out 3D gel cell culture and staining to get consistent 3D spheroid growth and staining for both
Matrigel and collagen I 3D gels.
• Created procedure for imaging and measuring blood vessels in dab stained slide.
• Debugged an immunofluorescence western blot technique and showed how changing to the Odyssey
system was not only more cost effective long term for the lab but also more environmentally friendly.
• Analyzed a historical mouse colony statistics to show that alpha2 integrin beta3 integrin double knock out
mice have a reduced litter size and frequency of litter.
• Discovered an error in the genotyping procedure used in the lab which resulted in 1 out of 4 heterozygote
mice being mistyped as knock out mice. Directed efforts to determine what inhibited the PCR reaction
and what would allow a functional and accurate genotyping procedure for a particularly difficult PCR.
• Determined that the lab inventory was out of date and reagents were lost or missing. Redesigned the old
Microsoft Access database in the more lab friendly Microsoft Excel and verified the current reagent
inventory.
FREEMAN PHOTOGRAPHERS, Nashville, TN
Data Manager
Input data into client database; created reports; backed up data.
• Developed and beta tested new Microsoft Access client database
2006-2009
MEHARRY MEDICAL COLLEGE, Nashville, TN
2005-2006
Research Assistant Professor
Project title: Quantifying the Contribution of TNF-alpha to Tumor Trowth and Angiogenesis Using In Vitro and In
Vivo Imaging Techniques
• Developed user manuals for microscope stations.
VANDERBILT, Nashville, TN
2002-2005
Postdoctoral Fellow, Cancer Imaging
Project title: Characterization of Tumor angiogenesis with MRI, Ultrasound and Optical Techniques
• Developed imaging technique for measuring tumor blood vascular volume
• Developed software analysis process for ultrasound quantitation of ovarian tumor blood volume
VANDERBILT, Nashville, TN
1999-2002
Postdoctoral Fellow
Project title: Development and characterization of EGFR Kinase Mutants
• Introduced new technique for making super competent cells producing rare 2KB DNA fragment bearing
plasmid
MEHARRY MEDICAL COLLEGE, Nashville, TN
Graduate Student
Project title: Interactions of Alpha-2HSGlycoprotein with Matrix Metalloproteinases
• Invented new procedure for isolating Fetuin from cultured cells or blood
• Creating Pictorial Equipment Manuals
• Developed user manuals for imaging stations.
EDUCATION
Doctorate in Philosophy, Biochemistry, Meharry Medical College
1993-1999
Bachelors of Science, Biology, University of Tennessee, Chattanooga
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AWARDS
2006 AACR Minority-Serving Institution Faculty Scholar Award in Cancer Research AACR Annual Meeting 2006
Travel Award
2005 AACR Minority-Serving Institution Faculty Scholar Award in Cancer Research “Anti-Angiogenesis and Drug
Delivery to Tumors: Bench to Bedside and Back” Travel Award
2004 SMI Young Investigator Travel Award
1999 Meharry Medical College Scientific Development Award
PUBLICATIONS:--JOURNAL ARTICLES
Wilson WD, Valet AS, Andreotti RF, Green-Jarvis B, Lyshchik A, Fleischer AC. Sonographic Quantification of Ovarian
Tumor Vascularity Journal of Ultrasound in Medicine 2006
L. Yang, L. DeBusk, K. Fukuda, B. Green-Jarvis, Y. Shyr, B. Fingleton, L. M. Matrisian, D. P. Carbone and PN. C. Lin,
Expansion of Myeloid Immune Suppressor Gr+CD11b+ Cells inTumor-bearing Host Directly Promote Tumor
Angiogenesis. Cancer Cell 2004 6: 409-421
Ochieng, J., Green, B., Evans, S., James, O., and Warfield, P. Modulation of the Biological functions of Galactin-3 by
Matrix MetalloproteinasesBiochemBiophysActa 1998 Jan8;1379(1):97-10
Ochieng, J., and Green, B. The Interactions of Alpha 2HS Glycoprotein with MetalloproteinasesBiochemMolBiolInt
1996 Sep;40(1):13-20.
Ochieng, J., Warfield, P., and Green., B. Interactions of Gelatinases with Soluble and Immobilized Fetuin and
AsialofetuinArch BiochemBiophys 1995 Sep 10;322(1):250-5.
PUBLICATIONS:--ABSTRACTS
Shelia Johnson, Brenda Jarvis, Billy Hudson,Celestial Jones-Paris, Bobby Madamanchi, Roberto Vanacore, Amber
Crabtree, Mary Zutter. The Expression of Collagen IV and Laminin V in Mammary Epithelium Vanderbilt
Aspirnaut Program 2014
Bobby Madamanchi, Norma Ramirez, Zhengzhi Li, Richard Friedman, Brenda Jarvis, Kent Dickeson, and Mary Zutter
Alpha2Beta1 and Anoikis – A Final Hurdle on the Road to Metastasis Vanderbilt Cancer Sympoisium 2012
Bobby Madamanchi, Norma Ramirez, Zhengzhi Li, Richard Friedman, Brenda Jarvis, Kent Dickeson, and Mary Zutter
Alpha2Beta1 and Anoikis – A Final Hurdle on the Road to Metastasis Vanderbilt Cancer Symposium 2011
Fleischer, A. C. ,Andreotti, R. F., Wilson, W. D., Valet, A.S, Green-Jarvis, B., and Lyshchik, A. Sonography of Ovarian
Tumors Using Quantitated Color Doppler Sonography vs Morphology AIUM 2007
O'Brien, C., Green-Jarvis, B., and Khabele, D. The Effects of Aspirin (ASA) and Paclitaxel (PTX) in COX-1 Expressing
Ovarian Cancer Cells Vanderbilt-Ingram Cancer Center Retreat 2006: Inflammation and Cancer
O'Brien, C., Green-Jarvis, B., and Khabele, D. The Effects of Aspirin (ASA) and Paclitaxel (PTX) in COX-1 Expressing
Ovarian Cancer Cells Meharry Medical College 50th Annual Student Research Day 2006
Green-Jarvis, B., O’Brien, C. Parl, A., Montgomery Rice, V. and Khabele, D. HDAC inhibitors and VEGF in Epithelial
Ovarian Cancer. James Symposium 2006
O'Brien, C., Green-Jarvis, B., and Khabele, D. The Effects of Aspirin (ASA) and Paclitaxel (PTX)in COX-1 Expressing
Ovarian Cancer Cells James Symposium 2006.
Wilson, Wendy D., Green-Jarvis, Brenda, and Fleischer, Arthur C. Sonographic Quantification of Ovarian Tumor
Vascularity 2006 Annual Meeting of WFUMB (World Federation for Ultrasound in Medicine and Biology)
Green-Jarvis, Brenda, O’Brien, Charmaine, Parl, Angelika, Montgomery Rice, Valerie, and Khabele, Dineo. HDAC
Inhibitors and VEGF in Epithelial Ovarian Cancer 2005 AACR Anti-Angiogenesis and Drug Delivery to Tumor:
Bench to Bedside and Back
Wilson, Wendy D., Green-Jarvis, Brenda, and Fleischer, Arthur C. Sonographic Quantification of Ovarian Tumor
Vascularity 2005 Annual Meeting of AIUM (Amer. Inst. of Ultrasound in Med.)
Green-Jarvis, B., Jacobs, P.M., Lee, H., Holburn, G., Lin, C, and Price, R A Technique for Monitoring Extracranial
Tumor Angiogenesis with a MRI Blood Pool Agent, Ferumoxytol 2005 AACR
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Li Yang, Laura DeBusk, Kori Fukuda, Barbara Fingleton, Brenda Jarvis-Green,Yu Shyr, Lynn Matrisian, David
Carbone, P. Charles Lin. Expansion of myeloid immune suppressorGr+CD11b+ cells in tumor-bearing host
directly promotes tumor angiogenesis 2005 AACR
Green-Jarvis, B., Lin, C, and Price, R.A Histological Method for Validation of Blood Volume Imaging Methods 2005
Cancer Imaging Retreat
Green-Jarvis, B., Lee, H., Holburn, G., Lin, C, and Price, R. A Technique for Monitoring Tumor Angiogenesis with
Ferumoxytol 2004 Society for Molecuar Imaging
Green-Jarvis, B., Price, R., Lee, H., Williams, M., Jacobs, P., and Lin, C. MRI Assessment of Tumor
Microvascularity2003 World Congress on Medical Physics and Biomedical Engineering
Green-Jarvis B., Yankeelov T, Luci J, Lin C, and Price R. MRI Assessment of Microvascularity Third Annual Joint HostTumor Interactions Program and Department of Cancer Biology Retreat November 21-22, 2003
Chavous TD, Gao W, Jarvis B, Buchowski MS. Markers of bone metabolism in sickle cell disease: Relationship to age
and gender. FASEB Journal, 1999; 13, 5:679.11
Green-Jarvis, B., King, A., Overall, C.M., and Ochieng, J. Interactions of Fetuin with Gelatinases Proceedings of the
American Association of Cancer Research Annual Meeting,1999; 40:4676
PUBLICATIONS:--ACKNOWLEDGEMENTS
Park, S., Wang, X., Chen, Z., Powers, A.C., Magnuson, M. A., Head, W. S., Piston, D. W., and Bell, G.I. Optical
Imaging of Pancreatic Beta Cells in Living Mice Expressing a Mouse Insulin I Promoter-firefly Luciferase
Transgene Genesis 43:80-86.
Das, S.K., and Murkerjee, S. Heterogeneity of human red blood cell membrane: Co-existence of heavy and light
membranes Molecular and Cellular Biochemistry 1999 196:141-49