Download Descargar PDF resumen

Grup de Sensors & Biosensors GSB
http://sensors.uab.es
Info: [email protected]
GSB
[Fundación (1983)]
Permanentes (6)
Contratados (4)
Postdocs (1)
Postgrados > 25
Salvador Alegret, CU
Jordi Bartrolí, CU
Julian Alonso, CU
Esteve Martínez, TU
Manel del Valle, TU
Francisco Céspedes, AU
Dmitri Muraviev, RyC
Ma. José Esplandiu, RyC
Ma. Isabel Pividori, LU
Mireia Baeza, LU
Arben Merkoçi*, ICN
GSB
taxonomía evolutiva
1983
Potentiometric
electrodes/
FIA
Sensors
1993
2003
GSB
1988
Biosensor
BioSens
ABI
Artificial
Biosensing
Interfaces
Integrated
Analytical
Systems
Converging
technologies
GRUP de SENSORS i BIOSENSORS - TECNOLOGIAS
TRANSDUCTORES
OPTICOS
SPR
SISTEMAS
ANALITICOS
INTEGRADOS
SENSORES FIBRA
OPTICA
SENSORES
OPTOELECTRONICOS
(BIO)
ANALIZADORES
AUTOMATIZADOS
ELECTRODOS
SELECTIVOS
DE IONES
TECNOLOGIA
CONVENCIONAL
TRANSISTORES
DE EFECTO
DE CAMPO
(ISFET)
TECNOLOGIA
MICROELECTRONICA
CROMOFOROS
MEMBRANAS
OPTICAS
TECNOLOGIA
BIOQUIMICA
IONOFOROS
POLIMEROS Y
COMPOSITES
MATERIALES
BIOCOMPOSITES
(BIO)ANALIZADORES
(BIO)ANALIZADORES
SENSORES
IMPEDIMETRICOS
DISPOSITIVOS
DISPOSITIVOS
1 SOLO USO
1 SOLO USO
(SERIGRAFIA)
(SERIGRAFIA)
LENGUAS
ELECTRONICAS
POTENCIOMETRICAS
ADITIVOS
MEMBRANAS
POTENCIOMETRICAS
ENZIMAS
ADN
ANTICUERPOS
SENSORES
SENSORESQUIMICOS
QUIMICOS
SENSORES
AMPEROMETRICOS
SISTEMAS
BIOMIMETICOS
QUÍMICA
FIA
SIA
ELECTRICOS
BIOSENSORES
BIOSENSORES
INMUNOSENSORES
INMUNOSENSORES
GENOSENSORES
GENOSENSORES
LENGUAS
ELECTRONICAS
VOLTAMPEROMETRICAS
INFORMATICA
NANOCOMPÓSITOS
NANOCOMPÓSITOS
NANOPARTICULAS
NANOPARTICULAS
NANOTUBOS
NANOTUBOSDE
DECC
CONVERGING TECHNOLOGIES
WILSON & WILSON’S
COMPREHENSIVE
ANALYTICAL CHEMISTRY
EDITED BY
D. BARCELÓ
INFO
VOLUME XXXIX
INTEGRATED ANALYTICAL SYSTEMS
NANO
BIO
BY
S. ALEGRET, Ed.
COGNO
NANO-BIO-INFO-COGNITIVE SYSTEMS
NANO-BIO-ANALYTICAL SYSTEMS
ELSEVIER
AMSTERDAM LONDON NEW YORK OXFORD PARIS SHANNON TOKYO
[email protected]
1. ION-SELECTIVE ELECTRODES (ISE)
PVC membrane / graphite-polymer composite
Tubular electrodes
Integrated flow-continuous systems
2. ION-SELECTIVE FIELD EFFECT TRANSISTORS (ISFET)
Chip (micro)
Probe (meso)
Gauge (macro)
3. ANALYSERS FOR ENVIRONMENTAL MONITORING BASED ON THE FIA
TECHNIQUE (FLOW INJECTION ANALYSIS) AND ON POTENTIOMETRIC
DETECTION
4. NETWORK OF AUTOMATIC STATIONS FOR WATER QUALITY CONTROL
In collaboration with ADASA Sistemas, S.A
[email protected]
Desarrollo y evaluación de analizadores automáticos para el
control de parámetros de interés medioambiental en aguas
Estación Automática de Control de la Calidad
RED AUTOMÁTICA DE MONITORIZACIÓN DE LA CALIDAD DE
LAS AGUAS (XACQA)
RÍO ANOIA EN MARTORELL
RÍO LLOBREGAT
EN CASTELLBELL
RIERA DE RUBÍ EN RUBÍ
RED AUTOMÁTICA DE MONITORIZACIÓN DE LA CALIDAD DE
LAS AGUAS (SAICA-EBRO)
EBRO EN MENDAVIA
ZADORRA EN ARCE
IREGUA EN ISLALLANA
EBRO EN MIRANDA
GÁLLEGO EN ZARAGOZA
[email protected]
5. MICROSISTEMAS DE ANALISIS TOTAL
Tecnología de silicio
VENTANAS PARA LA INTEGRACION
DE DETECTORES OPTICOS Y TIPO ISFET
MICROSISTEMAS DE ANALISIS TOTAL
ANALIZADOR DE ION PLOMO CON DETECCION POTENCIOMETRICA
Tecnología LTCC
Reference electrode
Ground
Ground
ISE
ISE connection to
external set-up
a
Hydraulic
circuit
Connections
b
Cl- solution
Carrier/Sample
c
Conditioning
solution
d
90
E= -69 + 33.log(ppm Pb 2+ + 367)
80
70
60
E (mV)
Waste
50
40
30
20
10
1,5
2,0
2,5
3,0
log (ppmPb2+)
3,5
4,0
4,5
MICROSISTEMAS DE ANALISIS TOTAL
ANALIZADOR DE ION NITRITO CON DETECCION COLORIMETRICA
2.6cm
DISPOSITIVO LTCC
Ventana
Óptica
Imagen RX
2.2cm
2.5
0,6
Abs=-3.31·10-3+0.048·[NITRITO]
2.0
ABSORBANCIA
0,5
Absorbance
0,4
0,3
0,2
0.1ppm
0.5ppm
1ppm
2ppm
5ppm
7ppm
10ppm
0,1
0,0
-0,1
0
20
40
60
80
100
Time (s)
120
140
160
180
1.5
Q=5.83µl/s
volumen muestra
y reactivo=29µl
Rango 0.1-10ppm
r2=0.9959
1.0
0.5
0.0
0
2
4
[NO
6
-]
2
(ppm)
8
10
12
6. INTEGRATED WAVEGUIDE ABSORBANCE OPTODES ACTIVATED WITH IONSELECTIVE MEMBRANES
In collaboration with the National Microelectronics Centre of Barcelona (CNM)
and the Optical Fibers Group from the University of Zaragoza
input Rib ARROW waveguide 10x4
microns
minimum losses 600-800 nm
Isolating cladding
cut waveguide zone
core elimination
membrane deposition 4 microns
output Rib ARROW waveguide
50x4 microns
no cladding
[email protected]
7. ELECTROCHEMICAL IMMUNOSENSING BASED ON AFFINITY PLATFORM
Graphite-polymer biocomposite
[email protected]
8. ELECTROCHEMICAL IMMUNOSENSING BASED ON mag-GEC
Magnetic beads / graphite-polymer composite
Magnetic
Particles
MP-COOH
MB-Tosyl
MB-ProtA
Size
(μm)
Surface Active
Group
0.196
Tx-HRP
Tx
Antibiotics
Pesticides
Covalent
Binding
2.8
2.8
Antibody
Coupling
Strategy
Covalent
Binding
(Protein A)
Pollutants
Affinity
Binding
The competitive assay is performed in a solution
containing:
For atrazine determination, specific antibodies
were covalently coupled to MB-Tosyl, MPCOOH and MB-ProtA magnetic beads. For
sulfonamides specific antibodies, Abs were
covalently coupled to MB-Tosyl magnetic
beads.
1.- Antibody modified magnetic particles
2.- Spiked sample
Tx
3.- Competitor Tracer (Tx-HRP)
TX-HRP
[email protected]
ELECTROCHEMICAL IMMUNOSENSING STRATEGY BASED ON mag-GEC
Magnetic beads / carbon-polymer composite
A
B
HRP-antiDig
digoxigenin
probe
mismatch
DNA
C
DNA
target
M-GEC
A) Magnetic beads incubations occurs in
solution (hybridization of DNA, enzyme or
antibody labeling) B) The capture of the
modified magnetic beads on the magneto
sensor is produced by the magnetic affinity
with the magnet inside the sensor body and C)
Schematic
representation
of
the
electrochemical strategy based on m-GEC
electrode.
magnetic
bead
Scanning electron microphotographs showing the
captured magnetic beads on the surface of m-GEC
magneto sensor.
Resolution: 2 µm (1), 10 µm (2), 50 µm (3), 100 µm (4) and
500 µm (5).
Acceleration voltage: 15 kV.
Number of magnetic beads: 6.2 x 106.
HRP
biotinylated
probe
HQ
ne-
streptavidin
magnet
m-GEC electrode surface
1
2
2 µm
3
5
10 µm
4
500 µm
50 µm
100 µm
H2O2
H 2O
9. ELECTROCHEMICAL GENOSENSING BASED ON
GOLD NANOPARTICLES
DIRECT VOLTAMMETRIC DETECTION
Physical
Adsorption
open circuit
Au0
Au0
Au0
Electrochemical oxidation
0.1 M HCl
Cleaning
+1.25 V
+1.25 V (vs. Ag/AgCl), 120 s
Electrochemical
Reduction
CV (+1.25 V to 0 V)
Au0
Au0
AuIII+ III+AuIII+
Au
M.Pumera, C.Mills, A.Merkoçi, S.Alegret; Electrochimica Acta, 2005, (published in Web )
[email protected]
Au-NPs (Ø < 50 nm) / MBs (Ø < 10 µm)
GOLD NANOPARTICLES. Direct electrochemical detection
Direct electrochemical detection of magnetically trigged
hybridization based Au67 – DNA – paramagnetic bead conjugate
A
C
B
Au
DNA
E
Au
Au
DNA2
DNA1
Au
Au
(A) introduction of streptavidin coated paramagnetic beads
(B) immobilization of the biotinylated probe (DNA2) onto the
paramagnetic beads
(C) addition of the 1:1 Au67-DNA1 target
(D) accumulation of Au67-DNA1/DNA2-paramagnetic bead
conjugate on the surface of magnetic electrode
(E) magnetically trigged direct DPV electrochemical
detection of gold quantum dot tag in
Au67-DNA1/DNA2-paramagnetic bead conjugate.
Langmuir, 21, 9625-9629 (2005)
D
Au
Au
Au
Au
Au
Au
Au
Au
[email protected]
10. ELECTROCHEMICAL SENSORS BASED ON CARBON NANOTUBES
CNT-polymer composite
Carbon nanotubes
integration in sensors
CNTs as surface modifiers
,
,
CNT composites
[email protected]
[email protected]
11. Matrices de sensores
Sistemas
Sistemas
biomimèticos
biomimèticos
Matriz de sensores
Ôe-nose
Ôe-tongue
Colaboraciones requeridas
Validación interlaboratorios de los prototipos desarrollados
en muestras reales
Desarrollo de instrumentación analítica