Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Grup de Sensors & Biosensors GSB http://sensors.uab.es Info: [email protected] GSB [Fundación (1983)] Permanentes (6) Contratados (4) Postdocs (1) Postgrados > 25 Salvador Alegret, CU Jordi Bartrolí, CU Julian Alonso, CU Esteve Martínez, TU Manel del Valle, TU Francisco Céspedes, AU Dmitri Muraviev, RyC Ma. José Esplandiu, RyC Ma. Isabel Pividori, LU Mireia Baeza, LU Arben Merkoçi*, ICN GSB taxonomía evolutiva 1983 Potentiometric electrodes/ FIA Sensors 1993 2003 GSB 1988 Biosensor BioSens ABI Artificial Biosensing Interfaces Integrated Analytical Systems Converging technologies GRUP de SENSORS i BIOSENSORS - TECNOLOGIAS TRANSDUCTORES OPTICOS SPR SISTEMAS ANALITICOS INTEGRADOS SENSORES FIBRA OPTICA SENSORES OPTOELECTRONICOS (BIO) ANALIZADORES AUTOMATIZADOS ELECTRODOS SELECTIVOS DE IONES TECNOLOGIA CONVENCIONAL TRANSISTORES DE EFECTO DE CAMPO (ISFET) TECNOLOGIA MICROELECTRONICA CROMOFOROS MEMBRANAS OPTICAS TECNOLOGIA BIOQUIMICA IONOFOROS POLIMEROS Y COMPOSITES MATERIALES BIOCOMPOSITES (BIO)ANALIZADORES (BIO)ANALIZADORES SENSORES IMPEDIMETRICOS DISPOSITIVOS DISPOSITIVOS 1 SOLO USO 1 SOLO USO (SERIGRAFIA) (SERIGRAFIA) LENGUAS ELECTRONICAS POTENCIOMETRICAS ADITIVOS MEMBRANAS POTENCIOMETRICAS ENZIMAS ADN ANTICUERPOS SENSORES SENSORESQUIMICOS QUIMICOS SENSORES AMPEROMETRICOS SISTEMAS BIOMIMETICOS QUÍMICA FIA SIA ELECTRICOS BIOSENSORES BIOSENSORES INMUNOSENSORES INMUNOSENSORES GENOSENSORES GENOSENSORES LENGUAS ELECTRONICAS VOLTAMPEROMETRICAS INFORMATICA NANOCOMPÓSITOS NANOCOMPÓSITOS NANOPARTICULAS NANOPARTICULAS NANOTUBOS NANOTUBOSDE DECC CONVERGING TECHNOLOGIES WILSON & WILSON’S COMPREHENSIVE ANALYTICAL CHEMISTRY EDITED BY D. BARCELÓ INFO VOLUME XXXIX INTEGRATED ANALYTICAL SYSTEMS NANO BIO BY S. ALEGRET, Ed. COGNO NANO-BIO-INFO-COGNITIVE SYSTEMS NANO-BIO-ANALYTICAL SYSTEMS ELSEVIER AMSTERDAM LONDON NEW YORK OXFORD PARIS SHANNON TOKYO [email protected] 1. ION-SELECTIVE ELECTRODES (ISE) PVC membrane / graphite-polymer composite Tubular electrodes Integrated flow-continuous systems 2. ION-SELECTIVE FIELD EFFECT TRANSISTORS (ISFET) Chip (micro) Probe (meso) Gauge (macro) 3. ANALYSERS FOR ENVIRONMENTAL MONITORING BASED ON THE FIA TECHNIQUE (FLOW INJECTION ANALYSIS) AND ON POTENTIOMETRIC DETECTION 4. NETWORK OF AUTOMATIC STATIONS FOR WATER QUALITY CONTROL In collaboration with ADASA Sistemas, S.A [email protected] Desarrollo y evaluación de analizadores automáticos para el control de parámetros de interés medioambiental en aguas Estación Automática de Control de la Calidad RED AUTOMÁTICA DE MONITORIZACIÓN DE LA CALIDAD DE LAS AGUAS (XACQA) RÍO ANOIA EN MARTORELL RÍO LLOBREGAT EN CASTELLBELL RIERA DE RUBÍ EN RUBÍ RED AUTOMÁTICA DE MONITORIZACIÓN DE LA CALIDAD DE LAS AGUAS (SAICA-EBRO) EBRO EN MENDAVIA ZADORRA EN ARCE IREGUA EN ISLALLANA EBRO EN MIRANDA GÁLLEGO EN ZARAGOZA [email protected] 5. MICROSISTEMAS DE ANALISIS TOTAL Tecnología de silicio VENTANAS PARA LA INTEGRACION DE DETECTORES OPTICOS Y TIPO ISFET MICROSISTEMAS DE ANALISIS TOTAL ANALIZADOR DE ION PLOMO CON DETECCION POTENCIOMETRICA Tecnología LTCC Reference electrode Ground Ground ISE ISE connection to external set-up a Hydraulic circuit Connections b Cl- solution Carrier/Sample c Conditioning solution d 90 E= -69 + 33.log(ppm Pb 2+ + 367) 80 70 60 E (mV) Waste 50 40 30 20 10 1,5 2,0 2,5 3,0 log (ppmPb2+) 3,5 4,0 4,5 MICROSISTEMAS DE ANALISIS TOTAL ANALIZADOR DE ION NITRITO CON DETECCION COLORIMETRICA 2.6cm DISPOSITIVO LTCC Ventana Óptica Imagen RX 2.2cm 2.5 0,6 Abs=-3.31·10-3+0.048·[NITRITO] 2.0 ABSORBANCIA 0,5 Absorbance 0,4 0,3 0,2 0.1ppm 0.5ppm 1ppm 2ppm 5ppm 7ppm 10ppm 0,1 0,0 -0,1 0 20 40 60 80 100 Time (s) 120 140 160 180 1.5 Q=5.83µl/s volumen muestra y reactivo=29µl Rango 0.1-10ppm r2=0.9959 1.0 0.5 0.0 0 2 4 [NO 6 -] 2 (ppm) 8 10 12 6. INTEGRATED WAVEGUIDE ABSORBANCE OPTODES ACTIVATED WITH IONSELECTIVE MEMBRANES In collaboration with the National Microelectronics Centre of Barcelona (CNM) and the Optical Fibers Group from the University of Zaragoza input Rib ARROW waveguide 10x4 microns minimum losses 600-800 nm Isolating cladding cut waveguide zone core elimination membrane deposition 4 microns output Rib ARROW waveguide 50x4 microns no cladding [email protected] 7. ELECTROCHEMICAL IMMUNOSENSING BASED ON AFFINITY PLATFORM Graphite-polymer biocomposite [email protected] 8. ELECTROCHEMICAL IMMUNOSENSING BASED ON mag-GEC Magnetic beads / graphite-polymer composite Magnetic Particles MP-COOH MB-Tosyl MB-ProtA Size (μm) Surface Active Group 0.196 Tx-HRP Tx Antibiotics Pesticides Covalent Binding 2.8 2.8 Antibody Coupling Strategy Covalent Binding (Protein A) Pollutants Affinity Binding The competitive assay is performed in a solution containing: For atrazine determination, specific antibodies were covalently coupled to MB-Tosyl, MPCOOH and MB-ProtA magnetic beads. For sulfonamides specific antibodies, Abs were covalently coupled to MB-Tosyl magnetic beads. 1.- Antibody modified magnetic particles 2.- Spiked sample Tx 3.- Competitor Tracer (Tx-HRP) TX-HRP [email protected] ELECTROCHEMICAL IMMUNOSENSING STRATEGY BASED ON mag-GEC Magnetic beads / carbon-polymer composite A B HRP-antiDig digoxigenin probe mismatch DNA C DNA target M-GEC A) Magnetic beads incubations occurs in solution (hybridization of DNA, enzyme or antibody labeling) B) The capture of the modified magnetic beads on the magneto sensor is produced by the magnetic affinity with the magnet inside the sensor body and C) Schematic representation of the electrochemical strategy based on m-GEC electrode. magnetic bead Scanning electron microphotographs showing the captured magnetic beads on the surface of m-GEC magneto sensor. Resolution: 2 µm (1), 10 µm (2), 50 µm (3), 100 µm (4) and 500 µm (5). Acceleration voltage: 15 kV. Number of magnetic beads: 6.2 x 106. HRP biotinylated probe HQ ne- streptavidin magnet m-GEC electrode surface 1 2 2 µm 3 5 10 µm 4 500 µm 50 µm 100 µm H2O2 H 2O 9. ELECTROCHEMICAL GENOSENSING BASED ON GOLD NANOPARTICLES DIRECT VOLTAMMETRIC DETECTION Physical Adsorption open circuit Au0 Au0 Au0 Electrochemical oxidation 0.1 M HCl Cleaning +1.25 V +1.25 V (vs. Ag/AgCl), 120 s Electrochemical Reduction CV (+1.25 V to 0 V) Au0 Au0 AuIII+ III+AuIII+ Au M.Pumera, C.Mills, A.Merkoçi, S.Alegret; Electrochimica Acta, 2005, (published in Web ) [email protected] Au-NPs (Ø < 50 nm) / MBs (Ø < 10 µm) GOLD NANOPARTICLES. Direct electrochemical detection Direct electrochemical detection of magnetically trigged hybridization based Au67 – DNA – paramagnetic bead conjugate A C B Au DNA E Au Au DNA2 DNA1 Au Au (A) introduction of streptavidin coated paramagnetic beads (B) immobilization of the biotinylated probe (DNA2) onto the paramagnetic beads (C) addition of the 1:1 Au67-DNA1 target (D) accumulation of Au67-DNA1/DNA2-paramagnetic bead conjugate on the surface of magnetic electrode (E) magnetically trigged direct DPV electrochemical detection of gold quantum dot tag in Au67-DNA1/DNA2-paramagnetic bead conjugate. Langmuir, 21, 9625-9629 (2005) D Au Au Au Au Au Au Au Au [email protected] 10. ELECTROCHEMICAL SENSORS BASED ON CARBON NANOTUBES CNT-polymer composite Carbon nanotubes integration in sensors CNTs as surface modifiers , , CNT composites [email protected] [email protected] 11. Matrices de sensores Sistemas Sistemas biomimèticos biomimèticos Matriz de sensores Ôe-nose Ôe-tongue Colaboraciones requeridas Validación interlaboratorios de los prototipos desarrollados en muestras reales Desarrollo de instrumentación analítica