Download TDS Dexamethasone

Dexamethasone
NF-kB and MAPK inhibitor
Catalog # tlrl-dex
For research use only
Version # 13K15-MM
PrODucT iNFOrMATiON
METHODS
content:
100 mg Dexamethasone provided lyophilized
Preparation of stock solution (1 mg/ml)
To obtain a 1 mg/ml stock solution:
1. Weigh 5 mg of dexamethasone.
2. Add 5 ml ethanol to 5 mg dexamethasone. This provides a
solution at 1 mg/ml (2.55 mM).
3. Vortex until complete solubilization.
4. Aliquote and store at 4˚C.
5. Once dexamethasone is solubilized, dilutions can be
prepared by adding sterile culture medium. To obtain a
solution at 20 mg/ml (51 mM), add 49 ml of culture medium
to 1 ml of the stock solution. Aqueous solutions should not be
stored for more than one day.
Storage and stability:
- Dexamethasone is shipped at room temperature. Store
lyophilized product at 4°C. Lyophilized product is stable for 1
year when properly stored.
- Solutions of dexamethasone are stable for 1 month at 4°C.
Protect from light.
DEScriPTiON
Dexamethasone is a synthetic glucocorticoid compound with
potent anti-inflammatory activities. It represses a large set of
pro-inflammatory genes by blocking NF-kB and MAPK
activation during TLR engagement inhibits NF-kB and
specific MAPKs depending on the TLR ligand and whether the
adapters MyD88 and TRIF are activated individually or
coincidently1.
Working concentration: 100 nM (40 ng/ml)
1. Bhattacharyya S. et al., 2010. TAK1 targeting by glucocorticoids
determines JNK and IkappaB regulation in Toll-like receptorstimulated macrophages. Blood. 115(10):1921-31.
NF-kB and MAPK inhibition:
To assess the role of NF-kB and MAPK, pretreat cells, such as
macrophages or HEK293 cells transfected with one or several
TLR genes, for 1 hour with or without dexamethasone
at 100 nM and then incubate at 37°C with the appropriate TLR
ligand for 1 to 6 hours. Detect NF-kB and MAPK inhibition
using the appropriate detection system.
cHEMicAl PrOPErTiES
rElATED PrODucTS
cAS number: 50-02-2
Synonym: Prednisolone F
Formula: C22H29FO5
Molecular weight: 392.46
approximately 25 mg/ml in ethanol
Solubility:Dexamethasone
Appearance: White powder
Product
pUNO1-hTLR2 (plasmid with human TLR2 gene)
puno1-htlr2
pUNO-mTLR2 (plasmid with mouse TLR2 gene)
puno-mtlr2
Piceatannol
Pam3CSK4 (TLR2 ligand)
tlrl-pms
pUNO-hTLR3 (plasmid with human TLR3 gene)
puno-htlr3
pUNO-mTLR3 (plasmid with mouse n°
TLR3
gene)
puno-mtlr3
CAS
10083-24-6
Poly(I:C) HMW (TLR3 ligand)
tlrl-pic
OH
Molecular Weight: 228,24
Chemical Formula: C14H12O3
OH
HO
OH
n° CAS 501-36-0
Resveratrol
Molecular Weight: 331,34
Chemical Formula: C20H14FN3O
F
N
OH
N
H
N
n° CAS 152121-30-7
SB202190
Molecular Weight: 475,62
Chemical Formula: C26H41N3O5
O
H
N
O
N
H
O
H
N
O
O
O
F
H 3C
HO
H
n° CAS 133407-82-6
MG 132
Molecular Weight: 392,46
Chemical Formula: C22H29FO5
H
Molecular Weight: 244,24
Chemical Formula: C14H12O4
HO
H
CH3
OH
CH3
OH
O
OH
OH
OH
n° CAS 50-02-2
Dexamethasone
n° CAS 10083-24-6
Piceatannol
n° CAS 50-02-2
OH
CH3
HO
H 3C
O
H
F
O
OH
CH3
OH
HO
H
TECHNICAL SUPPORT
Toll free (US): 888-457-5873
MG
Outside US:
(+1) 132
858-457-5873
Europe: +33 562-71-69-39 n° CAS 133407-82-6
E-mail: [email protected]
Website: www.invivogen.com
O
N
H
N
OH
Chemical Formula: C14H12O4
Molecular Weight: 244,24
Chemical Formula: C22H29FO5
Molecular Weight: 392,46
O
catalog code
O
N
3950 Sorrento Valley Blvd. Suite 100
San Diego, CA 92121 - USA
H