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Page 1 of 4 FORM NO.: F-013-00 SUPERSEDES DATE: New ISSUE DATE: Jan. 15, 2008 LIFELINE CELL TECHNOLOGY MATERIAL SAFETY DATA SHEET FOR HUMAN CELL CULTURES (Biosafety Level 2) Lifeline cultures are not hazardous as defined by OSHA 1910.1200. However, as live cells they are potential biohazards. SECTION 1 – PRODUCT AND COMPANY INFORMATION Product Name: Human Renal Medullary Epithelial Cells P1 Product Number: FC-0018 Company Address: Lifeline Cell Technology 32 East Frederick Street Walkersville, MD 21793 Technical Phone: Fax: Emergency Phone: (877) 845-7787 (301) 845-0885 (877) 845-7787 SECTION 2 – COMPOSITION / INFORMATION ON COMPONENTS Liquid Cell Culture: Either frozen or growing cells shipped in liquid cell culture medium (a mixture of components that may include, but is not limited to: inorganic salts, vitamins, amino acids, carbohydrates and other nutrients dissolved in water). Synonyms: Not Applicable. SECTION 3 – HAZARDS IDENTIFICATION Hazardous Ingredients: Frozen cultures may contain up to 10% Dimethyl sulfoxide (DMSO.) This cell line has been screened for Hepatitis B, Hepatitis C, HIV-1 and other adventitious agents. Potential Health Effects: Eye: May cause irritation of the eye. Skin: May cause skin irritation. Inhalation: May be irritating to mucus membranes and upper respiratory tract. Ingestion: May cause irritation if swallowed. SECTION 4 – FIRST AID MEASURES Oral Exposure: If swallowed, rinse out mouth with water provided person is conscious. Call a physician. FOR PRODUCT ORDERING CONTACT CUSTOMER SERVICE: 10 EAST FREDERICK STREET, WALKERSVILLE, MD 21793 TOLL FREE: (877) 845-7787 FAX: (301) 845-2405 WWW.LIFELINECELLTECH.COM Page 2 of 4 FORM NO.: F-013-00 SUPERSEDES DATE: New ISSUE DATE: Jan. 15, 2008 Dermal Exposure: In case of contact with skin, flush with copious amounts of water for at least 15 minutes. If irritation continues, call a physician. Eye Exposure: In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. If irritation continues, call a physician. Inhalation: If inhaled, remove to fresh air. If breathing is difficult, call a physician. SECTION 5 – FIRE FIGHTING MEASURES Materials: Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Wear self contained breathing apparatus for fire fighting if necessary. SECTION 6 – ACCIDENTAL RELEASE MEASURES Methods for Cleaning Up: Contain the spill and decontaminate using suitable disinfectants such as chlorine bleach or 70% ethyl or isopropyl alcohol. Wear chemical goggles, rubber boots, and rubber gloves. SECTION 7 – HANDLING AND STORAGE Handling: Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or repeated exposure. This cell line is of human origin and requires handling at Biosafety Level 2 containment [U.S. Government Publication Biosafety in Microbiological and Biomedical Laboratories (CDC, 1999)]. Cell lines may fall under regulation 29 CFR 1910.1030 Bloodborne Pathogens. Use universal precautions when handling this product. Further recommendations: Lifeline recommends that appropriate safety procedures be used when handling all cell lines, especially those derived from human material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice (Fleming, et al., 1995), the Journal of Tissue Culture Methods (Caputo, 1988), and in the U.S. Government Publication, Biosafety in Microbiological and Biomedical Laboratories (CDC, 1999). This publication is available in its entirety in the Center for Disease Control Office of Health and Safety’s web site at http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm. Storage: Keep container tightly closed. Store below -135°C. Protect from light. SECTION 8 – EXPOSURE CONTROLS AND PERSONAL PROTECTION Engineering Controls: Safety shower and eye wash stations are required. Use of Certified Biological Safety Cabinet is recommended. Ventilation: Area ventilation is generally adequate. Personal Protective Equipment: Respiratory: Respirator not required. FOR PRODUCT ORDERING CONTACT CUSTOMER SERVICE: 10 EAST FREDERICK STREET, WALKERSVILLE, MD 21793 TOLL FREE: (877) 845-7787 FAX: (301) 845-2405 WWW.LIFELINECELLTECH.COM Page 3 of 4 FORM NO.: F-013-00 SUPERSEDES DATE: New ISSUE DATE: Jan. 15, 2008 Hand: Compatible chemical resistant gloves required. Eye: Safety glasses or goggles required. General Hygiene Measures: Wash hands thoroughly after handling. SECTION 9 – PHYSICAL / CHEMICAL PROPERTIES Appearance: Colored liquid, color may vary with pH. SECTION 10 – STABILITY AND REACTIVITY Stability: Stable. Hazardous polymerization will not occur. SECTION 11 – TOXICOLOGICAL INFORMATION Primary Irritant Effect: This product is a potential irritant to eyes and skin. This product may be harmful if ingested or inhaled. This cell line has been screened for Hepatitis B, Hepatitis C, HIV-1 and other adventitious agents. Cell lines may fall under regulation 29 CFR 1910.1030 Bloodborne Pathogens. Complete toxicological properties have yet to be determined. SECTION 12 – ECOLOGICAL INFORMATION Not Applicable. SECTION 13 – DISPOSAL CONSIDERATIONS Appropriate Method of Disposal: Dispose of cultures and exposed materials by autoclaving at 121°C for 20 minutes or exposing to 10% chlorine bleach for at least 10 minutes. Follow all Federal, State and local regulations. SECTION 14 – TRANSPORT INFORMATION Not Applicable. SECTION 15 – REGULATORY INFORMATION Not Applicable. SECTION 16 – OTHER INFORMATION Disclaimer: This product is for research and development use only. Not for clinical or household use. FOR PRODUCT ORDERING CONTACT CUSTOMER SERVICE: 10 EAST FREDERICK STREET, WALKERSVILLE, MD 21793 TOLL FREE: (877) 845-7787 FAX: (301) 845-2405 WWW.LIFELINECELLTECH.COM Page 4 of 4 FORM NO.: F-013-00 SUPERSEDES DATE: New ISSUE DATE: Jan. 15, 2008 Warranty: The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regards to appropriate safety precautions. Lifeline Cell Technology reserves the right to revise this MSDS periodically as new information becomes available. It does not represent any guarantee of the properties of the product. Lifeline Cell Technology shall not be held liable for any damage resulting from handling or from contact with the above product. FOR PRODUCT ORDERING CONTACT CUSTOMER SERVICE: 10 EAST FREDERICK STREET, WALKERSVILLE, MD 21793 TOLL FREE: (877) 845-7787 FAX: (301) 845-2405 WWW.LIFELINECELLTECH.COM