Download Giulio Amadei, Ph.D.

Giulio Amadei, Ph.D.
Research Fellow
Cancer Research Laboratory Program
790 Commissioners Road E.
London, ON N6A 4L6
CANADA
Work: (+1) 519 685 -8600 x 53317
Home: (+1) 519 474-9007
E-mail:ma il@g iu lioamadei.com
CAREER PROF ILE
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Ph.D. level synthetic organic and medicinal chemist. Experience in solution and solid-phase multi-step
syntheses, modern structural elucidation methods, separation and characterization techniques, catalysis,
reaction kinetics, process and product development. Excellent knowledge of GLP and SLP regulations.
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Extensive hands-on research experience in various chemistry related fields (medicinal chemistry,
environmental chemistry, and bio -inorganic chemistry) in both academic and government settings.
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Proven abilities to quickly become expert in new subjects and techniques, to identify most important
concepts and information, and to troubleshoot problems. Excellent administrative, interpersonal, and
communication skills. Creative, analytical, focused and detailed oriented.
EDUCATION
1996–2000
Georgetown University, Washington, DC
PhD Dec. 2000 - (Organic Chemistry)
MS Nov. 1998 - (Organic Chemistry)
1987–1993
Universita' Studi Milano, Milano, Italy
BS Mar. 1993 – (Synthetic Organic Chemistry)
1981–1984
Istituto Tecnico Industriale Statale (State Industrial
Institute), Milano, Italy
Engineering technical diploma (Specialization in Industrial Chemistry)
P ERSONAL DETAILS
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Citizenship: Italian
Canada status: Permanent resident of Canada
Giu lio Amadei, Ph.D. E-mail: ma il@g iu lioamadei.com
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WORK HISTORY & ACCOM P LISHM ENTS
2007 -date
Research Fellow
(Radiology & Nuclear Medicine)
London Regional Cancer Program – London, ON − Canada
Peptides as probes to target the epidermal growth factor-like domain 7 (EGFL7) for non-invasive in
vivo imaging of angiogenesis
2006–2007 Research Associate
(Bio-inorganic Chemistry, and Coordination Chemistry)
Laboratoire de Chimie de Coordination du CNRS − Toulouse − France
Ongoing studies on the potency and modes of action of
Pt(II)–Cu(II) complexes of
phenanthroline–based ligands as interesting novel artificial nucleases. The characterization of Pt(II)
anchorage point(s) onto the oligonucleotide targets (14 -36 mers) was achieved by two independent
methods: enzymatic digestion of the formed Pt(II) adduct(s) (HPLC and LC/MS analyses), and Taq
DNA polymerase primer extension experiments (PAGE anal
The characterization of Cu(II)
oxidative cleavage point(s) was accomplished by PAGE analysis. (12 –month project)
2001–2006 Postdoctoral Fellow
(Medicinal Chemistry)
Structural Biophysics Laboratory − Molecular Aspects of Drug Design
National Cancer Institute, National Institutes of Health, Frederick, MD
Found a feasible solution to the MDRI (Multi Drug Resistance) problem affecting patients under
chemotherapy drug regimens by rational design (hydropathy analysis) and synthesis of polypeptides
mimicking transmembrane domains of ABCG2 transport protein. (3 –year project)
Successfully produced a novel class of antibiotics by ational design (computational modeling) of
inhibitors of HPPK enzyme. Structural features of the
compound were established by using
available X-ray crystal structure data of ternary HPPK−Inhibitor−ATP complexes. (2 –year project)
1996–2000 Graduate Research and Teaching Assistant
(Inorganic Chemistry, Synthetic Organic Chemistry, and Environmental Chemistry)
Georgetown University − Chemistry Department, Washington, DC
Rational design of novel tetraaza macrocyclic ligands, and their Ti(III) complexes, as a practical way
to destroy perchlorate ions, widespread contaminants of ground water in the North American
continent. (3–year project)
PATENT: Georgetown University, USA − PCT Int. Appl. 2001, 33 pp.
Earley, J. E., Sr.; Amadei, G. A.; Tofan, D. E.“Catalytic System and Method for Reducing Perchlorate
Ion”
1991–1993 Unde rgraduate Research Assistant
(Synthetic Organic Chemistry)
Universitá Studi Milano, Milano – Italy
The regulation of the conjugate addition of metal enolates of ketones and esters to Michael acceptors
was successfully accomplished by implementation of chiral and achiral Ti(IV) “ate” complexes of
those compounds. Additions of such complexes (generated in situ) to Michael acceptors, were highly
regio– and stereo selective, showing an improved 1,4 –regio selectivity when compared to the lithium
enolate counterparts. (18–month project)
Giu lio Amadei, Ph.D. E-mail: ma il@g iu lioamadei.com
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P RACTICAL SKILLS
Chemistry (Organic and Inorganic)
Synthesis
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Organic and inorganic multi-step synthetic sequences (Aliphatic, aromatic, organometallic, heterocyclic,
and coordination compounds)
Metal template synthesis of tetraaza macrocyclic ligands
Solid-phase synthesis of supported reagents (on PVA matrices)
Stereoselective reactions (i.e., asymmetric epoxidation, asymmetric conjugate additions)
Micro– and semi–microscale reactions
Physical methods
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Multinuclear FT–NMR (1H; 13C; 19F; 31P; 195Pt) and 2D experiments (i.e., HETCOR, COSY)
FT–IR; UV–Vis spectroscopies
Fluorescence spectroscopy (FluoMax 2 system)
Spectrometry: tandem GC –MS; LC –MS, MALDI–TOF [small molecules (AXIMA–CFR™ Plus system)]
Electronic circular dichroism
Reaction kinetics experiments & homogeneous and heterogeneous catalysis
X-Ray crystallography (Crystal growth techniques)
Purification techniques
TLC analytical and preparative
Flash chromatography manual and automatic (ISCO system)
Ion exchange chromatography
NP –, and RP–HPLC (Agilent Technologies & Waters systems)
LC–MS (Agilent Technologies)
Medicinal Chemistry & Molecular Biology
Development of biology protocols as applied to drug discovery
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High-throughput drug activity assays (peptides, oligonucleotides)
In -vitro drug toxicity testing
Bioinformatics as applied to drug discovery
Peptide synthesis protocols [Linear synthesizer (ABI)]
Combinatorial peptide library synthesis protocols [Parallel synthesizer APEX 396 (Advanced ChemTech)]
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Labeling protocols (γ– P–ATP), anealation, and denaturation
Chemical and enzymatic sequencing
Enzymatic degradation (DNaseI, S1 and P1 nucleases, BAP dephosphorylation)
Drug–DNA target interactions (Taq DNA polymerase experiments)
Basic oligonucleotides protocols
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Basic cell culture protocols [fixed (HL60), and in-suspension (SF295) cell lines]
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Propagation and maintenance
Viability essay (MTT, Trypan Blue)
Flow cytometry
Protein overexpression
Separation, characterization, and analytical techniques
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Affinity, and size–exclusion chromatography
MALDI–TOF with PSD studies (protein, peptides, oligonucleotides) (AXIMA–CFR™ Plus system)
PAGE (peptides, proteins, oligonucleotides)
RP–HPLC (peptides, proteins, and oligonucleotides)
Bidimensional FT–NMR experiments (peptide conformation/aggregation studies in phospholipid bilayers)
Giu lio Amadei, Ph.D. E-mail: ma il@g iu lioamadei.com
P UBLICATIONS AND PRESENTATIONS
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Platinated Copper(3 -Clip-Phen) Complexes as Effective DNA-Cleaving and Cytotoxic Agents
Özalp-Yaman, S.; de Hoog, P.; Amadei, G.; Pitié, M.; Gamez, P.; Dewelle, J.; Mijatovic, T.; Meunier, B.; Kiss, R.;
Reedijk, J. Chemistry 2008, 14(11), 3418
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DNA cleavage and binding selectivity of a heterodinuclear Pt–Cu(3-Clip-Phen) complex
DeHoog, P.; Pitié, M.; Amadei, G.; Gamez, P.; Meunier, B.; Kiss, R.; Reedijk, J.
J. Biol. Inorg. Chem. Manuscript DOI 10.1007/s00775-008 -0346-y on-line Feb 2008
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Towards the Discovery of a Potent HPPK Inhibitor
Amadei, G.; Park, M.; Ji, X.; Yan, H., M, Michejda, C. J.
Conference : Interd isciplinary Retreat, Rocky Gap, Cumberland, MD, 2005
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Toward a Feasible Solution to the Multi Drug Resistanc Problem
Amadei, G.; Tarasova, N. I.; Nelson, C.; Michejda, C. J.
Structural Biophysics Laboratory, NCI-Frederick, P.O. BOX B, Frederick, MD 21702
Conference: Third NCI Fellows & Young Investigators Retreat, Ocean City, MD, 2003
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Transmembrane inhibitors of polytopic membrane proteins
Tarasova, N. I.; Amadei, G.; Seth, R.; Hrycyna, C. A.; Gottesman, M. M.; Michejda, C. J.
Structural Biophysics Laboratory, NCI-Frederick, Frederick, MD, USA.
Abstracts of Papers, 224th ACS National Meeting, Boston, MA, United States, August 18-22, 2002, MEDI-407
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Effect of some macrocyclic ligands on the rate of reduction of perchlorate ion by titanium(III)
Amadei, G. A.; Earley, J. E., Sr.
Department of Chemistry, Georgetown University, Washington, DC, USA.
Croatica Chemica Acta 2001 , 74(3), 601-606
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Reduction of perchlorate ion by titanous ions in ethanolic solution
Earley, J. E., Sr.; Tofan, D. C.; Amadei, G. A..
Department of Chemistry, Georgetown University, Washington, DC, USA.
Environmental Science Research 2000 , 57(Perchlorate in the Environment), 89-98.
Publisher: Kluwer Academic/P lenum Publishers
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Reduction of perchlorate ions by titanous complexes
Earley, J. E.; Amadei, G. A..
Chemistry Department, Georgetown Univers ity, Washington, DC, USA.
Book of Abstracts, 219th ACS National Meeting, San Francisco, CA, March 26-30, 2000 , INOR-577.
Publisher: American Chemical Society, Washington, D. C
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Synthesis of the {Co[(6 -amino-6-(4-aminobenzyl) -1,4,8,11 -tetraazacyclotetradecane)][PO4]}, a potential
phosphate-binding site for molecular recognition
Amadei, G. A.; Earley, J. E.
Department of Chemistry, Georgetown University, Washington, DC, USA
Inorg. Chimica Acta 1999 , 293(2), 245-247.
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Rapid reduction of perchlorate ion by titanous ion in ethanolic solutions
Earley, J. E., Sr.; Tofan, D. C.; Amadei, G. A..
Department of Chemistry, Georgetown University, Washington, DC, USA.
Preprints of Extended Abstracts presented at the ACS National Meeting, American Chemical Society, Divis ion of
Environmental Chemistry 1999 , 39(2), 90-92.
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Synthesis of 6 -amino-6-(4-aminobenzyl)-1,4,8,11 -tetra-azacyclotetradecane, a new pentadentate polyamine
ligand, and crystal structure of its Co(III) complex
Amadei, G. A.; Dickman, M. H.; Wazzeh, R. A.; Dimmock, P.; Earley, J. E.
Department of Chemistry, Georgetown University, Washington, DC, USA.
Inorg. Chimica Acta 1999 , 288(1), 40-46.
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Giu lio Amadei, Ph.D. E-mail: ma il@g iu lioamadei.com
AF FILIATION IN SCIENTIF IC SOCIETIES
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American Chemical Society
Sigma Xi–Georgetown University Chapter (Full member)
Canadian Society for Chemistry
REF ERENCES
Available upon request
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