Download Zopiclone/Eszopiclone

Meprobamate
ELISA Kit
Zopiclone/Eszopiclone
ELISA Kit
Simple Sensitive Solutions for Forensic Drug Detection
Summary
Product Features
Zopiclone and eszopiclone are non-benzodiazepine hypnotic
agents. They are used in the treatment of insomnia in humans.
Currently, eszopiclone is being marketed within the US as
Lunesta by Sepracor Inc. Zopiclone, manufactured by SanofiAventis, is only available outside of the U.S. and sold under
several other brand names including Imovane and Zimovane.
• Detects zopiclone and eszopiclone
• Ultra sensitive detection
• Validated for human urine, whole blood and serum
• Single and bulk packaging available
• Compatibility with automated and semi-automated instruments
Zopiclone and eszopiclone are currently classified schedule IV
under the Controlled Substance Act in the U.S. The potential
for abuse and toxicity due to overdose has been observed for
both drugs.Human urine and blood remain important matrix
types for testing of drugs of abuse. These two drugs can be
found in both matrices as parent and metabolite forms.
Data from European Medicine Agency Study 194-026 suggest
concentrations of eszopiclone below 10 ng/mL in the plasma
have minimal effect on the EEG and cognitive impairment.
The point at which eszopiclone plasma concentrations fall
to 10 ng/mL is approximately 8 hours following a 3.5 mg
eszopiclone dose. This study supports the development of a
highly sensitive enzyme-linked immunosorbent assay (ELISA)
for the detection of zopiclone and eszopiclone in human urine
and blood.
References
Baselt, R.C. Disposition of toxic drugs and chemicals in man, 7th edition, Foster City, California:
Biomedical Publications, 2004.
Moffat, A.C. Osselton, M.D., Widdop, B. Clarke’s Analysis of Drugs and Poisons, 3rd edition, London,
UK: Pharmaceutical Press, 2004.
Summary of Protocol
- Add 20 µL / 50 µL samples and controls
- Add 100 µL ready-to-use enzyme conjugate solution
- 45 minute incubation at room temperature
- Wash plate 3 times
- Add 100 µL Substrate TMB
- 30 minute incubation at room temperature
- Add 100 µL Stop Solution
- Measure OD at 450 nm
Sensitivity and Specificity
Cross-Reactivity Profile
Drug
I-50 (ng/mL)
Percent Cross-Reactivity
Zopiclone
3.5
100
Eszopiclone
1.8
194.4
N-desmethyl-zopiclone
3.5
350
Zopiclone-N-Oxide
3.5
100
6-(5-Chloro-2-pyridyl)-6,7dihydro-7-hydroxy-5H-pyrrolo[3,4b]
pyrazin-5-one
800
0.4
Withdrawal Assessment Report for Lunivia. 2009, European Medicines Agency. 1-101.
Clarke, J. Zopiclone/eszopiclone forensic assay review. Neogen Corporation, 2013.
Sample Dilutions
The recommended sample dilution is 1:10 for urine, 1:5 for serum and 1:20
for whole blood.
Order Details
Single Kit - 96 well package
Bulk Kit - 480 well package
For Forensic Use Only
Product No. 133819
Product No. 133815
Neogen Corporation
944 Nandino Blvd., Lexington, KY 40511 USA
Phone: 859/254-1221
Phone: 800/477-8201 (USA/Canada)
Fax: 859/255-5532
Orders/Email: [email protected]
Web: www.neogen.com/Toxicology
©Neogen Corporation, 2013. Neogen is a registered trademark of Neogen Corporation, Lansing, Michigan. All other trademarks are the property of their respective owners.
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