Download AFB Stain Kit Procedure for Raymond A Lamb STAINMATE

AF.B. STAIN KIT PROCEDURE
Optimized for Raymond A Lamb STAINMATE™ Automated Stainer
Item# KTA202
KIT
COMPONENTS
INCLUDED:
1% ACID ALCOHOL
ADVANCED CARBOL FUCHSIN
(Revised 04/28/11)
LIGHT GREEN COUNTERSTAIN
STORAGE NOTE: STORE ADVANCED CARBOL FUCHSIN AT ROOM TEMPERATURE.
PRODUCT NOTE: ADVANCED CARBOL FUCHSIN SHOULD BE FILTERED IF A “GOLD SHEEN" FROM
OXIDATION DEVELOPS ON THE SURFACE.
PRINCIPLE: This kit demonstrates Acid Fast Bacteria and Tubercle Bacilli.
SPECIMEN: Any well fixed paraffin embedded tissue cut at 6 microns.
QUALITY CONTROL: American Master*Tech Scientific Recommended Control Slide:
Acid Fast Bacteria, CSA0125P
PROCEDURE:
1. Deparaffinize slide with Xylene or Xylene Substitute and hydrate through alcohols.
STAINMATE™
2. Rinse
slide in PROGRAMMING
Tap water. STEPS
STEP
#
BATH
#
TIME
IN BATH
(SECONDS)
AGITATION
IN BATH
YES/NO
TIME
OVER BATH
(SECONDS)
AGITATION
OVER BATH
YES/NO
COMPONENTS
00
01
02
03
04
05
06
07
08
09
10
11
1
3
3
3
3
2
2
2
3
4
5
5
900
1
1
1
180
10
10
10
60
60
60
0
NO
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
NO
5
1
1
1
5
1
1
5
5
5
0
0
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
NO
NO
ADVANCED CARBOL FUCHSIN
RUNNING TAP WATER
RUNNING TAP WATER
RUNNING TAP WATER
RUNNING TAP WATER
1% ACID ALCOHOL
1% ACID ALCOHOL
1% ACID ALCOHOL
RUNNING TAP WATER
LIGHT GREEN
REAGENT ALCOHOL
REAGENT ALCOHOL*
* Following STAINMATE™ Step 11; For optimal staining results slide should be removed from Reagent Alcohol within
3 to 4 minutes from the STAINMATE™ Automated Stainer and proceed manually to procedure Step 3.
3. Dehydrate slide through 2 changes of Absolute Alcohol.
4. Clear slide through 3 changes of Xylene or Xylene Substitute.
5. Coverslip using a permanent mounting media.
RESULTS:
Tubercle Bacilli, other Acid Fast Bacteria: BRIGHT RED
Other Tissue: LIGHT GREEN
LEPRA BACILLUS DEMONSTRATION, NOCARDIA DEMONSTRATION:
To demonstrate Lepra Bacillus, use AMT Leprosy STAINMATE Procedure, Fite's Stain Kit.
To demonstrate Nocardia, use AMT Nocardia STAINMATE Procedure, Fite's Stain Kit.
Human Spleen
REFERENCE: A.F.I.P. Manual, pg. 176; Mallory Pathological Technique, pg. 273. Automated staining procedure
modified by American MasterTech.
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