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STUDY GUIDE Plant Life Cycles In lab, we tried to copy down and make sense of the life cycles of mosses, ferns, gymnosperms, and angiosperms. This study guide will hopefully help you understand plant life cycles and their evolution. Understanding Haploidy, Diploidy, and Alternation of Generations One way to better understand the life cycles of plants is to compare them to the life cycles of humans. Remember from prior lectures and labs that a diploid cell is one that has two sets of chromosomes (2n chromosomes), one set inherited from each parent. A haploid cell is one that has only one set of chromosomes (n chromosomes.) In humans, sperm and eggs are haploid. Study Question 1: Are most of our cells haploid or diploid? What is the type of cell division that can turn a diploid cell into a haploid cell? Study Question 2: Do our sperm and eggs divide into multicellular organisms before fertilization? Does the egg divide after it has been fertilized? What does fertilization do to the chromosome number in an egg? Do humans normally have any multicellular haploid phase in their life cycle? Alternation of Generations: Instead of producing sperm or egg directly, meiosis in plants in the diploid sporophyte stage produces spores. These are single cells which can be male or female and can divide. When these spores divide by mitosis, they make haploid gametophytes. It is the gametophytes that produce sperm or eggs. Sperm can fertilize eggs, and the resulting zygote can develop into a new sporophyte. In other words, while humans directly create gametes by meiosis, and these gametes can be fertilized without dividing further, plants reproduce differently. In plants, there's a delay and a great deal of mitosis before gametes are produced to be fertilized. This delay is the gametophyte generation. Plants alternate between sporophyte and gametophyte generations. STUDY GUIDE Plant Life Cycles Study Question 3: In humans, we can get, at most, four sperm from a single meiosis event. Is this true in plants, which use meiosis to produce male and female gametophytes, which then divide more to produce sperm and eggs? Why or why not? Alternation of Generations in Bryophytes: In bryophyte mosses, the gametophyte is the majority of the moss's structure, near the ground. It produces eggs and sperm in special chambers, and sperm can be dispersed from one gametophyte to another by rain or other water to fertilize an egg. Once an egg is fertilized, it grows into a diploid sporophyte that is still attached to the gametophyte. That sporophyte makes haploid spores that become new gametophytes. Alternation of Generations in Ferns: In ferns, unlike bryophytes, the gametophyte stage is reduced to a small prothallus. Fertilization occurs under or near the ground, and the resulting sporophyte is the majority of the plant. A big, leafy fern we see in the woods is a sporophyte, and its spores can be found under its leaves. Study Question 4: On land, there's a higher chance of sunlight and other environmental dangers causing mutations. Suppose a mutation "breaks" one copy of a gene at some random point in a plant's life. Which kind of plant, a bryophyte or a fern, would seem to be at more risk of suffering severe consequences from losing one working copy of a gene? Why? Alternation of Generations in Gymnosperms and Flowering Plants: Matters become a little more complicated when we reach the first seed plants. In gymnosperms, as in ferns, the sporophyte is the main phase of the life cycle. However, the haploid gametophyte is even less prominent. We'll use pines, STUDY GUIDE Plant Life Cycles a conifer, as our example. A pine tree is mostly diploid sporophyte, and makes male and female cones In the male cones, meiosis occurs to give rise to spores, and these spores give rise to pollen. This pollen is the male gametophyte, making sperm cells. In the female cones, meiosis also gives rises to spores, but the gametophyte those spores make is contained entirely inside the ovule (future seed). This female gametophyte produces an egg that is fertilized by the sperm cells, giving rise to a new sporophyte embryo. In other words, the gametophyte stage of a gymnosperm is reduced to a tiny portion of the life cycle - pollen and part of the ovule. Flowering plants are similar to gymnosperms in this way; they, too, reduce the role of the gametophyte to pollen and the egg-producing cells of the future seed. One difference is that in flowering plants, the seed is protected in an ovary. Study Question 5: Is the outside of a pine cone diploid or haploid? What about the inside of a mature pine seed? The inside of a pollen grain? The petals of a flower? The seed of an apple? Summary Table: Group of Plants Bryophyte mosses Ferns Gymnosperms Angiosperms Mostly gametophyte or mostly sporophyte? Where is/are the gametophyte(s)? Where is the sporophyte?