Download Nelly Aggangan - IUFRO Working Party 2.09.02

Fourth
International
Conference
of the IUFRO
Working
Party 2.09.02
Somatic Embryogenesis and Other
Vegetative Propagation Technologies
Sept. 19-23, 2016 – La Plata, Argentina
Nelly Siababa AGGANGAN
National Institute of Molecular Biology and
Biotechnology (BIOTECH)
University of the Philippines Los Baños
Philippines
[email protected]
Biography
Dr. Nelly S. Aggangan has been a researcher of the National Institute of Molecular Biology and
Biotechnology (BIOTECH), University of the Philippines Los Baños, College, Laguna, Philippines since 1980.
She is Scientist 1 under the Science Career Service of the Department of Science and Technology. She
holds a PhD degree in Forest Biotechnology from Murdoch University, Perth, Western Australia; MS and
BS degrees in Forestry from UPLB. She also had a two year Postdoctoral Fellowship at the National
Institute of Forest Sciences (NIFoS, formerly Korea Forest Research Institute) in Suwon, South Korea
where she developed protocol on mycorrhizal inoculation of somatic embryogenesis derived microplants.
Currently, she is doing similar researches on the application of selected mycorrhizal fungi in combination
with other beneficial microorganisms on micro and macropropagated plants (fruit crops and reforestation
species) and on raised seedlings.
Dr. Aggangan’s research activities address ecosystem renewal and sustainable productivity through
the development and commercialization of various microbial-based (bio)fertilizers and related studies.
She worked mainly on producing and commercializing mycorrhizal fungi that are effective in promoting
plant growth, survival and yield of high value crops and forest tree species. She has isolated and produced
mycorrhizal fungi that are tolerant to different environmental stresses and as biocontrol to root
pathogens such as nematodes and Fusarium wilt causing organisms. She also developed a new, easier,
cheap and more efficient production protocol, light weight and soilless carrier for both local and
international use.
http://www.iufro20902.org
Fourth
International
Conference
of the IUFRO
Working
Party 2.09.02
Somatic Embryogenesis and Other
Vegetative Propagation Technologies
Sept. 19-23, 2016 – La Plata, Argentina
Mycorrhization improved growth and survival of somatic
embryogenesis-derived
microplants
from
Kalopanax
septemlobus and Liliodendron tulipifera
In order to establish some cultural practices that can improve growth and survival of somatic
embryogenesis-derived microplants during acclimatization period, two concurrent experiments were
conducted. Kalopanax septemlobus and Liliodendron tulifipera microplants were uninoculated or inoculated
with arbuscular mycorrhizal fungi (AMF) during transfer from aseptic culture to individual container
filled with either sterile or non-sterile peat perlite vermiculite medium. Kalopanax were inoculated with
unidentified species of Glomus and Acaulospora (AMM6) collected in Bonghwa closed mine tailings, Korea
and Glomus etunicatum from the Philippines. Liliodendron microplants were inoculated with G. etunicatum,
G. macrocarpum and Gigaspora margarita. After one month incubation in acclimatization room, the
seedlings were transferred in a glasshouse. At this stage, inoculated plants were greener, with broader
leaves and well developed root system and had higher (90-95%) survival than the uninoculated ones. At
harvest, inoculated Kalopanax and Lilidendron were 50% and 2-4x (depending on the mycorrhizal species
studied), respectively, heavier than the uninoculated counterpart when grown in sterile medium. Plant
growth was better in sterile than in non-sterile medium throughout the five months observation period. G.
etunicatum consistently promoted the highest plant height, diameter, leaf length, leaf width, and dry
weight and nutrient uptake in both sterile and non-sterile medium. On the other hand, control plants
consistently had the lowest growth and nutrient uptake. G. macrocarpum and G. margarita produced
higher percent root colonization than G. etunicatum but these were less effective in promoting plant
growth and nutrient uptake. In conclusion, better growth and survival were obtained in sterile medium
than in non-sterile one. AMF inoculation is important in improving growth, survival and nutrient status of
SE-derived K. septemlobus and L. tulipifera. G. etunicatum promoted the highest leaf, stem, root and total
plant dry weight in both sterile and non-sterile medium. Further experimentations are needed to confirm
these initial findings.
Authors: Aggangan NS1, HK Moon2, YW Kim2 and SH Han2. 1National Institute of Molecular Biology
and Biotechnology, University of the Philippines Los Banos, College, Laguna 4031 Philippines
Email: [email protected]; 2 National Institute of Forest Sciences (NIFoS), Suwon 441-847, Korea;
http://www.iufro20902.org