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Fourth International Conference of the IUFRO Working Party 2.09.02 Somatic Embryogenesis and Other Vegetative Propagation Technologies Sept. 19-23, 2016 – La Plata, Argentina Nelly Siababa AGGANGAN National Institute of Molecular Biology and Biotechnology (BIOTECH) University of the Philippines Los Baños Philippines [email protected] Biography Dr. Nelly S. Aggangan has been a researcher of the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Baños, College, Laguna, Philippines since 1980. She is Scientist 1 under the Science Career Service of the Department of Science and Technology. She holds a PhD degree in Forest Biotechnology from Murdoch University, Perth, Western Australia; MS and BS degrees in Forestry from UPLB. She also had a two year Postdoctoral Fellowship at the National Institute of Forest Sciences (NIFoS, formerly Korea Forest Research Institute) in Suwon, South Korea where she developed protocol on mycorrhizal inoculation of somatic embryogenesis derived microplants. Currently, she is doing similar researches on the application of selected mycorrhizal fungi in combination with other beneficial microorganisms on micro and macropropagated plants (fruit crops and reforestation species) and on raised seedlings. Dr. Aggangan’s research activities address ecosystem renewal and sustainable productivity through the development and commercialization of various microbial-based (bio)fertilizers and related studies. She worked mainly on producing and commercializing mycorrhizal fungi that are effective in promoting plant growth, survival and yield of high value crops and forest tree species. She has isolated and produced mycorrhizal fungi that are tolerant to different environmental stresses and as biocontrol to root pathogens such as nematodes and Fusarium wilt causing organisms. She also developed a new, easier, cheap and more efficient production protocol, light weight and soilless carrier for both local and international use. http://www.iufro20902.org Fourth International Conference of the IUFRO Working Party 2.09.02 Somatic Embryogenesis and Other Vegetative Propagation Technologies Sept. 19-23, 2016 – La Plata, Argentina Mycorrhization improved growth and survival of somatic embryogenesis-derived microplants from Kalopanax septemlobus and Liliodendron tulipifera In order to establish some cultural practices that can improve growth and survival of somatic embryogenesis-derived microplants during acclimatization period, two concurrent experiments were conducted. Kalopanax septemlobus and Liliodendron tulifipera microplants were uninoculated or inoculated with arbuscular mycorrhizal fungi (AMF) during transfer from aseptic culture to individual container filled with either sterile or non-sterile peat perlite vermiculite medium. Kalopanax were inoculated with unidentified species of Glomus and Acaulospora (AMM6) collected in Bonghwa closed mine tailings, Korea and Glomus etunicatum from the Philippines. Liliodendron microplants were inoculated with G. etunicatum, G. macrocarpum and Gigaspora margarita. After one month incubation in acclimatization room, the seedlings were transferred in a glasshouse. At this stage, inoculated plants were greener, with broader leaves and well developed root system and had higher (90-95%) survival than the uninoculated ones. At harvest, inoculated Kalopanax and Lilidendron were 50% and 2-4x (depending on the mycorrhizal species studied), respectively, heavier than the uninoculated counterpart when grown in sterile medium. Plant growth was better in sterile than in non-sterile medium throughout the five months observation period. G. etunicatum consistently promoted the highest plant height, diameter, leaf length, leaf width, and dry weight and nutrient uptake in both sterile and non-sterile medium. On the other hand, control plants consistently had the lowest growth and nutrient uptake. G. macrocarpum and G. margarita produced higher percent root colonization than G. etunicatum but these were less effective in promoting plant growth and nutrient uptake. In conclusion, better growth and survival were obtained in sterile medium than in non-sterile one. AMF inoculation is important in improving growth, survival and nutrient status of SE-derived K. septemlobus and L. tulipifera. G. etunicatum promoted the highest leaf, stem, root and total plant dry weight in both sterile and non-sterile medium. Further experimentations are needed to confirm these initial findings. Authors: Aggangan NS1, HK Moon2, YW Kim2 and SH Han2. 1National Institute of Molecular Biology and Biotechnology, University of the Philippines Los Banos, College, Laguna 4031 Philippines Email: [email protected]; 2 National Institute of Forest Sciences (NIFoS), Suwon 441-847, Korea; http://www.iufro20902.org