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FROG DISSECTION PROCEDURE: • GO to the website for the virtual dissection: http://www.mhhe.com/biosci/genbio/virtual_labs/BL_16/ BL_16.html • PLACE the frog in the dissecting pan belly side up. • FOLLOW along with the steps of the virtual lab to start your dissection. • USE tweezers to lift the THIN skin away from the body cavity. • CUT the same way to remove the THIN layer of muscles so that you can see the organs. • If there are fatty bodies (bright orange, yellow greasy noodles), use your scissors to CUT them away. Be careful that you cut out the fatty bodies and NOT the intestines! • If you have a female frog you may see hundreds of eggs which look like black dots. REMOVE these eggs. • IDENTIFY each of the structures in the table below and check the box to show you’ve found them. o ASK for help from your teacher if you cannot identify a structure. • FOLLOW the guidelines below to begin your dissection. • LAY OUT the structures on a paper on your table to show your teacher as you cut them away STRUCTURE / ORGAN GALL BLADDER LIVER HEART and LUNGS I HAVE FOUND IT WHAT DOES IT LOOK LIKE AND WHERE IS IT? HOW DO I REMOVE IT? Under the liver there will be a Use scissors to carefully dark green small “grape” which cut it out stores the liquid bile Use scissors to carefully Brown colored large structure cut it out. Do not damage with three lobes (parts) the organs underneath! At the top of the liver there is a small triangular structure which Use scissors to cut both is the heart. Next to it are two the heart and lungs out. little bags which are the lungs I HAVE REMOVED IT FROG DISSECTION (continued) STRUCTURE / ORGAN I HAVE FOUND IT WHAT DOES IT LOOK LIKE AND WHERE IS IT? HOW DO I REMOVE IT? I HAVE REMOVED IT Remove the “food tube” from the frog by cutting at the top and bottom of it. You may also need to cut connective tissue. Stretch the tube out and MEASURE the following lengths: DIGESTIVE SYSTEM Under the liver you should Length of frog = ______cm now see the entire digestive tube. Length of tube =_____cm Length of small intestines =______cm STOMACH SMALL INTESTINE LARGE INTESTINE IDENTIFY the stomach, small intestine, and large intestine Cut lengthwise along the stomach to This leads into the small see if there is any food inside the intestines. stomach! Notice the stomach musclesthey looked ribbed. Notice the very small blood vessels The long thin tube that exits that are around the small intestine. the stomach This is where take the digested food is taken into the blood stream. The wider tube that leads from the small intestine Cut into the large intestine to see if there is any waste (poop) there!! Remove the KIDNEYS, which are two red organs located against the back of the frog’s abdomen. - These can be removed by gently lifting them and cutting through the membrane that’s attached to the back. On both sides of the spine and extending down to the legs, you will find NERVE BUNDLES that look like little white threads. - If you follow up the spine and look at the back on both sides of it, you will find more small bundles of nerves branching out on both sides.