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CHAPTER 1 INTRODUCTION A. Background of the Study Our planet Earth is called a green planet, mainly because it contains life.. Everything we eat comes directly or indirectly from plants. Plants are sources of oxygen. They are also used as medicine. Many other products, come from plants and shape up our lives like lumber, fuel, paper, fabrics, beverages, and spices. Nowadays, people mostly grow plants like vegetables in their garden to reduce the expense they spend in the market. But, some of these plant owners are cigarette users. Sometimes, they throw their cigarette butts or their unfinished lighted cigarette on the soil near the plant. This can cause soil pollution. There are over 4,000 chemicals in cigarettes which could have various toxic, mutagenic and carcinogenic effects. Three of the most widely known chemicals are nicotine, carbon monoxide, and tar. Nicotine is a mild stimulant found in tobacco plants. It is a strong poisonous drug and it is the main ingredient in insecticides. Carbon monoxide is a poisonous gas which is also found in the exhaust of a car. Tar is the oily material which remains after tobacco passes through the filter. These chemicals which cause soil pollution, also cause diseases among human beings who are directly or indirectly affected with cigarette filters which are designed absorb vapor and to accumulate particulate smoke components. Filters also prevent tobacco from entering a smoker's mouth and provide a mouthpiece. As the cigarettes contain many toxic substances that harm the environment especially the soil, the researchers decided to study the effects of extracted chemicals from cigarette butts in tap water since cigarette butts also contain chemical components as the smoke passes through the filter as soil contaminant on mongo plants. B. Statement of the Problem This study aims to answer the following questions: Main Problem: Can the chemicals present in the cigarette butts cause physical changes to mongo plants? Sub-problems: 1. What are the physical characteristics of mongo plants exposed to tap water with no chemicals from the cigarette butts in terms of: a. Leaf Color b. Number of leaves c. Plant Height d. Plant Weight 2. What are the physical characteristics of mongo plants exposed to the 1st squeezed cigarette butts in tap water as soil contaminant in terms of: a. Leaf Color b. Number of leaves c. Plant Height d. Plant Weight 3. What are the physical characteristics of mongo plants exposed to the 2nd squeezed cigarette butts in tap water as soil contaminant in terms of: a. Leaf Color b. Number of leaves c. Plant Height d. Plant Weight 4. What are the physical characteristics of mongo plants exposed to the 3rd squeezed cigarette butts in tap water as soil contaminant in terms of: a. Leaf Color b. Number of leaves c. Plant Height d. Plant Weight 5. What are the physical characteristics of mongo plants exposed to the 4th squeezed cigarette butts in tap water as soil contaminant in terms of: a. Leaf Color b. Number of leaves c. Plant Height d. Plant Weight 6. Is there a significant difference in the physical characteristics of mongo plants exposed to the 1st, 2nd, 3rd, and 4th squeezed cigarette butts in tap water as soil contaminant? 7. Is there a significant difference in the physical characteristics of mongo plants exposed to tap water and squeezed cigarette butts in tap water as soil contaminant? C. Objectives of the Study This research aims to: 1. determine if the chemicals present in cigarette butts can cause physical changes in mongo plants in terms of: a. Leaf Color b. Number of leaves c. Plant Height d. Plant Weight 2. compare the physical changes of mongo plants exposed to tap water from the extracted chemicals from cigarette butts with the 1st, 2nd, 3rd, and 4th attempt of squeezing exists. 3. determine if a significant difference in the physical characteristics of mongo plants exposed to tap water in 1st, 2nd, 3rd, and 4th attempt of squeezing exists. 4. determine if a significant difference in physical characteristics of mongo plants exposed to tap water and squeezed cigarette butts in tap water as soil contaminant exists. D. Hypotheses of the Study 1. There is no significant difference in the physical characteristics of mongo plants exposed to the 1st, 2nd, 3rd, and 4th squeezed cigarette butts in tap water as soil contaminant. 2. There is no significant difference in the physical characteristics of mongo plants exposed to tap water and squeezed cigarette butts in tap water as soil contaminant. E. Significance of the Study Many hazardous substances are present in cigarettes that are frequently disposed of by pouring them down drains, or even dumping them directly into the ecosystem, that can allow chemicals to seep into land or water supply that plants depend on. This study relates to the important issue of pollution and of caring the environment. The study aims to determine and identify the effects of cigarette butts to plants. Future researchers can use the data and results as basis to support their study. Furthermore, environmentalist, household members, students and smokers can acquire knowledge and be aware of the effects of cigarette butts to the environment especially to plants. F. Scope and Limitations of the Study The researchers will focus on the physical changes of mongo plants such as the color of the leaves, number of leaves, height of the plant, and weight of the plant. This study will limit to the use of cigarette butts, mongo plants and tap water. There will be 15 mongo plants to be tested on each set-up, one exposed to t the 1st, 2nd, 3rd, and 4th squeezed cigarette butts in tap water while the other is only exposed to tap water. Each mongo plant will be watered with 100ml of tap water. The researchers will determine the mongo plants' biomass to get the weight of the plants. The experimentation will be held on the researchers' residences. G. Definition of Terms Cigarette It is a small roll of finely cut tobacco leaves cylinder of thin paper for smoking. The cigarette is ignited at one end and allowed to burn slowly without flame; its smoke is inhaled from the other end, which is held in or to the mouth and in some cases a cigarette holder may be used as well. Cigarette Butt It is the remains of cigarette after smoking. It consists of a tissue tube which holds a filter. It typically comprises about 30% of the cigarette’s original length. In this study, chemicals squeezed from the cigarette butts are the contaminants. Mongo (Vigna radiata) It is a fast growing plant, also known as green bean. This plant will be used in this study. Biomass It is a biological material derived from living, or recently living organisms, in the context of biomass for energy that is often used mean plant based material, but biomass can equally apply to both animal and vegetable derived material. Contaminant It is a biological, chemical, physical, radiological substance (normally absent in the environment) which, in sufficient concentration, can adversely affect living organisms through air, water, soil, or food. CHAPTER II REVIEW OF RELATED LITERATURE AND STUDIES Plants grow everywhere and are essential to our lives as we use them as food, drugs, and fibers for cloth, and wood for energy and building materials. Other food either comes directly or indirectly from animals which exist only because they feed on or by eating plants or by eating other animals, which in turn ultimately depend on plants. Plants also provide essential habitat and food for many species of animals. Plants produce oxygen which is necessary for many forms of life on earth and they are important link in the global budget for carbon dioxide, which plays a major role in the "greenhouse effect" associated with global warming. Plants are important part of our aesthetic experience. We use them to decorate our streets, parks, yards and homes and many people find great pleasure and recreation in observing wildflowers and natural plant communities. Plants are also popular subjects for photographers and artists. (University of Wisconsin Green Bay, 2006) With the many benefits that we get from plants, we need to see to it that our our soil is healthy for our own plants to grow and to provide our needs. But one plant, tobacco is really harmful for some plants. The plants in the tobacco family, like tomato, potato and several flowering plants like Nicotiana, can get a leaf blight-any blight causing a browning and falling of the leaves of a plant- from coming in contact with cigarettes, butts, or even hands that have recently touched cigarettes. These are some evidences of bad effects brought by tobacco plant on other neighbouring plants.( Contents of Cigarette Butts, Yahoo.com , 2008) Traditional butts are made of “synthetic polymer cellulose acetate” and never degrade. These butts take up approximately 12 years to break apart. Yet within an hour of contact with water cigarette butts can begin teaching chemicals such as cadmium, lead, and arsenic into the marine environment. (One huge problem, Two solutions, August 2005) Arsenic comes in two forms: Organic and Inorganic. It is the organic that can cause health risks. Arsenic present in soil can prove to be dangerous to humans. (The Effects of Arsenic on Plant Surrounding, January 2004) In the case of cigarette smokers, every time a smoker lights up, they are not only filling their lungs with over four thousand chemicals, but the air around them as well. While the smoke of one cigarette may not contribute much to air pollution, when you consider the amount of smokers in the world and the number of cigarettes they smoke a day, the problem becomes far more serious for planet earth. The cigarette’s contribution to pollution does not end with the air. Discarded cigarette butts again may seem like a small problem until you consider how many there are. It’s estimated that there are millions of them lying on the ground and in the water – each one taking an estimated twenty-five years to decompose. When cigarette butts wind up in the water, small animals and waterfowl mistake them food. This often causes them to die. Furthermore, while in the water, the chemicals leak directly out into the body of water. (Articlesbase.com, 2010) There are so much more on the side effects of cigarette as there are harmful chemicals in one cigarette butt. Among the volatile gaseous phase of tobacco are nitrosamines, sulfur containing compounds, and hydrocarbons. Nicotine is a colorless volatile liquid alkaloid found in smoking and smokeless tobacco which turns brown and acquires the odor of the tobacco upon exposure to the air. (Tobacco, yaia.com, 2001) Cigarettes are made from four components: 1. Filters; 2. Tobacco; 3. Additives; and 4. Cigarette Wrapper. Filters found in cigarettes are specifically designed to absorb vapors and to accumulate smoke components. Filters also prevent tobacco from entering a smoker's mouth and provide a mouthpiece that will not collapse as the cigarette is smoked. 95% of cigarette filters are made of cellulose acetate (a plastic), and the balance are made from papers and rayon. The cellulose acetate tow fibers are thinner than sewing thread, white, and packed tightly together to create a filter; they can look like cotton. Other materials have been tried and rejected in favor of the taste that acetate produces. Filters vary in filtration efficiency, depending on whether the cigarette is to be "light" or regular. The paper used to wrap the acetate cellulose plug is impervious to air for regular cigarettes, or is ventilated and very porous in "light" cigarettes, allowing more air to enter the smoke mix. A polyvinyl acetate emulsion is used as the glue to attach the plug to the wrapper and to seam the wrapper.The tipping paper, often printed to look like cork, covers the filter plug and attaches the filter to the column of tobacco. Tipping paper is formulated to not adhere to the lips of smokers. (Cigarette Butt Litter, longwood.edu, 2000) The cigarette was redesigned and a new filter was added to most cigarettes. Filters were found to absorb and remove vapors and various constituents of cigarette smoke, such as tar and nicotine, in machine measure analyses. (National Cancer Institute [NCI], 2001) Tobacco and Nicotine in Cigarettes, the cultivated tobacco, Nicotiana tabacum, is a member of the nightshade family of plants. It is a broad leaf native of tropical America that is cultivated as an annual. Depending on the type of tobacco and its growing location, the leaves of the tobacco plant have different tastes, burning properties, aromas, color, and nicotine content. Tobacco leaves contain several alkaloids (see below), including the highly toxic alkaloid nicotine. Nicotine is the drug in tobacco that causes addiction in smokers according to the US Department of Health and Human Services. Nicotine is a powerful insecticide and among the deadliest of all plant products in its pure form. According to the US Department of Health and Human Services, it raises blood pressure, affects the central nervous system, and constricts blood vessels in humans. Nicotine is a colorless liquid that is highly soluble in water, and is readily absorbed through the skin in its pure form. In tobacco, there is much nicotine component which can also be found on other chemical forms. (Cigarette Butt Litter, longwood.edu, 2000) There are potentially hundreds of additives are mixed with tobacco during the manufacturing process. Additives to smoking tobacco include flavorings and humectants that are used to keep tobacco moist. According to a publication written for the tobacco industry, additives can constitute ten percent of the weight of the "tobacco" portion of a cigarette, and four per cent of the entire cigarette. From the additives, a complete list of 1,400 potential tobacco additives, include sweeteners and flavors such cocoa, rum, licorice, sugar, and fruit juices is considered a trade secret. Since tobacco is not classified as a food or drug, there are no legal maximums on agricultural chemicals or chemical additives cigarettes may contain. One of the widely used cigarette additive is menthol with its ability to provide flavor and to serve as an anesthetic. When burned, many additives form new compounds, possessing unique properties. For example, glycerol produces acrolein, a chemical which has been found to interfere with the normal clearing of the lungs. (Whelan, 1984) There is a need to create a filter to minimize harmful effects of cigarette. The cigarette was redesigned and a new filter was added to most cigarettes. Filters were found to absorb and remove vapors and various constituents of cigarette smoke, such as tar and nicotine, in machine measure analyses. (National Cancer Institute[NCL], 2001) More so, Nicotine is a mild stimulant found in tobacco plants, and can be smoked or chewed. It contains thousands of carcinogenic substances, most notably carbon monoxide and tar. Carbon monoxide is poisonous and found to be around 200 times more absorb-able into the blood stream than oxygen, therefore reducing the amount of oxygen available to the body through the bloodstream. Tobacco has been found to cause cancer, narrow the arteries and generally leads to a declining health due to its numerous substances taking their toll on the body. Nicotine is smoke to produce a mild relaxing effect and is believed to induce a feeling of psychological dependence. (Biologyonline.org, 2008) There was a comparative study conducted comparing cigarette to other materials as shown below: Familiar Chemicals in Cigarettes Chemical Found in: carbon monoxide car exhaust nicotine bug sprays tar material to make roads arsenic rat poison ammonia cleaning products hydrogen cyanide gas chamber poison cyanide deadly poison acetone nail polish remover butane cigarette lighter fluid DDT insecticides formaldehyde to preserve dead bodies sulfuric acid car batteries cadmium used to recharge batteries freon damages earth's ozone layer geranic acid a fragrance methoprene a pesticide maltitol a sweetener not permitted to be used in foods in the U.S. Sources: Dr. Joel Dunnington, Tobacco Almanac, Revised, May 1993. Another is the "tar" which is often referred to in connection with cigarettes is not a black petroleum tar product, but instead refers to the hundreds of substances and additives found in tobacco. Tar, when cool, is a sticky yellow-brown substance and the U.S. Department of Health and Human Services states that it is composed of organic and inorganic chemicals, including some carcinogens. The U.S. Federal Trade Commission defines tar as "total particulate matter…less nicotine and water." A similarly affecting chemical present in a cigarette, is the alkaloids, which according to the Concise Oxford Dictionary of Botany, are.. “a group of basic nitrogenous compounds of a complex nature. They are derived from plants and have powerful pharmacological effects. More than 1000 alkaloids are known from 1200 plant species. Their function is uncertain but in some species, they confer a degree of protection from insect attack. Pharmacologically powerful alkaloids derived from plants include cocaine, morphine, and strychnine, but they are not good when they touch the soil, they could kill the plants.” (Cigarette Butt Litter, longwood.edu, 2000) Considering the harmful effects of cigarette on the soil, they also ruin the water, even the human beings. Cigarette butts can take up to 12 months to break down in fresh water and up to 5 years to breakdown in seawater. These include chemicals such as carbon monoxide, hydrogen, cyanide, nitrogen oxides, polycyclic aromatic hydrocarbons, ammonia, acetaldehyde, formal dehyde, benzene, phenol, argon, pyridines, and acetone, over fifty of which are known to be carcinogenic to humans.( Elli Slaughter, Fall 2010) With excessive pollution derived from cigarette, either the soil, or the whole planet earth environment is greatly affected. Approximately 5 million hectares (600 million trees) of forest are destroyed each year to provide trees to dry tobacco. This is same size of 3.6 million Aussie Rules Football fields. If butts from these cigarettes were placed end to end, they would circle the planet 16 times. (Oxygen.org.au, 2006) CHAPTER III METHODOLOGY A. Research Design The study will deal with the determination of the effects of squeezed cigarette butts on the physical characteristics of mongo plants such as the color and number of leaves, height and weight of the plants. Two set-ups will be prepared, one will serve as the control group and the other as an experimental group that is exposed to squeezed cigarette butts in tap water as soil contaminant. Each set-up will have 15 mongo plants to be tested. The amount of water that will be used to every attempt of squeezing will be the same. The researchers will spend three weeks to observe changes in the physical appearance of the mongo plants. The data on the changes of physical characteristics of mongo plants will be recorded on a weekly basis. B. Materials/Equipment Materials: Equipment: Mongo Seeds Beaker Deep containers Gloves Soil Rack Tap Water Tape Measure Cigarette Butts C. General Procedure Collection of Materials Preparation of Squeezed Cigarette Butts in Tap Water In preparing the squeezed cigarette butts in tap water, the researchers will measure 100 ml of tap water for each set-up. Each set-up in the experimental group will only have the same 100 cigarette butts that will be used. And these cigarette butts in tap water will be squeezed 4 times only. Monitoring of Plant Growth D. Experimental Setup Table 1 Table on the Different Set-ups Used Set-ups Amount of Tap Water Number of Mongo Plants Number of Cigarette Butts A Control Group 100 ml 15 none B 1st Squeeze 100 ml 15 100 pieces C 2nd Squeeze 100 ml 15 100 pieces D 3rd Squeeze 100 ml 15 100 pieces E 4th Squeeze 100 ml 15 100 pieces E. Statistical Tools for Data Analysis