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Refining dwarf galaxias (Galaxiella pusilla) habitat in the Upper Barwon River catchment` A report for the Corangamite Catchment Management Authority Prepared by: Environous 42b Fenton Way, Hillarys, WA, 6025 Telephone: (08) 6201 0808 Email: [email protected] Produced for: Corangamite Catchment Management Authority 64 Denis Street, Colac, Victoria, 3250 This publication may be cited as: Ryan, T., Saddlier, S. and Scarborough, T. (2013) Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment. Report for the Corangamite Catchment Management Authority. Cover photographs: Clockwise from upper left: a) Site 1 (Callahans Ck offtake; b) dwarf galaxias with caudal fin damage (Site 18); c) Site 19 - billabong on East Barwon River; d) mountain galaxias captured from site 3; e) walking down onto the East Barwon River floodplain; f) southern pygmy perch captured at site 5; g) dwarf galaxias captured from site 9; and h) dense vegetation at site 17, This document was produced by e n v i r o n o u s Pty Ltd. for the Corangamite CMA and may only be used for the purposes and in accordance with the Terms of the Engagement for which it was commissioned. Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment EXECUTIVE SUMMARY The aim of this study was to investigate other potential dwarf galaxias wetland habitat in the upper Barwon River catchment in an attempt to find additional populations. This process was intended to improve the level of understanding required for the development of the new Corangamite Waterway Strategy. It also provided a re-assessment of important sites identified in previous processes such as a review of Significant Fish Species Protection under Dry Inflow Conditions (Saddlier et. al. 2009) and HERO Significant Fish Project (CCMA 2010). Dwarf galaxias is considered to be a non-migratory species adapted to wetland environments and is able to survive short periods with very little water (Saddlier et. al. 2009). In these habitats, it is typically found in marginal vegetation in still or slow moving waters of roadside ditches, swamps and backwaters of creeks. It is considered ‘Vulnerable’ in Australia (EPBC Act 1999), ‘Vulnerable’ in Victoria (DSE 2009) and is listed under the Victorian Flora and Fauna Guarantee Act 1988. As non-migratory species, the protection of primary refuge wetland habitat for dwarf galaxias is the most important management priority. This refuge habitat must provide shelter from high stream flows, sustained water quality and suitable instream habitat to provide shelter, feeding and spawning opportunities. Dispersion of these species from the refuge habitat is expected to occur predominately in a downstream direction. Throughout this investigation, where primary refuge habitat is identified, it is considered to have a higher management priority. Identification of these important primary offstream refuge and spawning sites should be a priority. Prior to this study there was only one recognised population of dwarf galaxias within the Upper Barwon River catchment in a wetland on Gosling Creek. An additional 20 wetlands and stream sites were surveyed using basic fish surveys, water quality and habitat assessments. Prioritisation of these sites were made according to a set of parameters, including: • previously identified threatened fish populations; • newly determined dwarf galaxias populations; • presence/absence of eastern gambusia (Gambusia holbrooki); • presence/absence stock exclusion fencing; • relative quality of riparian and instream habitat; • security from disturbance; • security of water supply; and • connectivity to main watercourse. An additional primary dwarf galaxias refuge wetland was identified on the southeast side of the old railway bridge on the East Barwon River floodplain near Barwon Downs. It is essential that a comprehensive maintenance plan be developed and includes stock exclusion fencing, weed and pest protection, protection of existing water resources and flow regime and re-vegetation of the riparian zone. It is also important to undertake ongoing water quality and non-destructive fish surveys to assess the sustainability of the populations. Other secondary populations of dwarf galaxias were identified at another wetland near the old railway bridge (East Barwon) – NW (site 18) and the ‘excavated billabong’ (site 19). Unfortunately, the high numbers of eastern gambusia found at these sites negated the many positive aspects of these sites (such as water permanency, good habitat quality). It should however, be recognised that due to the lack of offstream billabongs in the Upper Barwon Catchment, these sites provide shelter for many fish species in times of high and low stream flow, and should be maintained and enhanced as a matter of priority. Another key conservation measure noted for dwarf galaxias is the translocation of fish into a conservation wetland. Key criteria for conservation wetlands for dwarf galaxias is similar Environous report for Corangamite Catchment Management Authority i Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment to the above criteria but also considers the location of the site as a suitable demonstration / educational site. According to these criteria, the five potential conservation wetlands have been ranked in order of suitability and management practicality. The higher ranked wetlands are not impacted by eastern gambusia, and appear to have sufficient elevation and currently support predatory native species capable of controlling an invasion of eastern gambusia. They also appear to have sufficient water depth and water security and therefore require less restoration, apart from stock exclusion fencing and riparian vegetation planting. These sites include the Billabong on Mahers Road (Site 5) and the Bird Reserve on Pennyroyal Valley Road (Site 11). Other potential conservation wetlands (Wetland on Wendy James property - Site 3 and the Dry Conservation Reserve on Muroon Road - Site 16) would require excavation and further investigation into the water availability. The wetlands at Pennyroyal Creek Cottages (Site 12) provided a range of potentially suitable dwarf galaxias habitat, but were not considered suitable due the occurrence of eastern gambusia, Recommendations 1. 2. 3. 4. 5. 6. 7. ii Maintain and where possible without disturbance, enhance habitat at the Old Railway Bridge (East Barwon) – SE (Site 17). (Actions to include permanent fencing, erosion control on the East Barwon, and Glyceria control); Continue encouragement of landholder participation in stock exclusion fencing of the Gosling Creek wetland (Site 9); Undertake regular water quality, habitat condition and fish composition assessments at sites 17 and 9; Investigate feasibility of developing conservation wetlands (sites 3, 5, 11, 16) Undertake stock exclusion fencing and native planting at other floodplain wetlands (including sites 18, 19); Wetlands assessed in this investigation (sites 5,9,11,16,17,18,19) be adopted and implemented into the Corangamite Waterway Strategy (2013 -2019) as key conservation features of the upper Barwon Catchment and Assess other floodplain wetlands in the Upper Barwon catchment based on the criteria in this report. Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment TABLE OF CONTENTS Introduction ....................................................................................................................... 1 Background ..................................................................................................................... 1 Methods ............................................................................................................................. 2 Site Selection ................................................................................................................... 2 Aquatic Fauna Surveys .................................................................................................... 4 Results ............................................................................................................................... 5 Sites 1 and 2: Callahan Creek offtake .............................................................................. 6 Site 3: Wetland at Wendy James ..................................................................................... 7 Site 4: Causeway at Wendy James.................................................................................. 8 Site 5: Billabong on Mahers Road .................................................................................... 9 Site 6 and 7: Dewing Creek Weir and Rowlands Road .................................................. 10 Site 8: Dewing Creek Weir Offtake................................................................................. 11 Site 9: Gosling Creek Wetland ....................................................................................... 12 Site 12: Pennyroyal Road Cottages ............................................................................... 15 Site 13: Pennyroyal Railway dam................................................................................... 16 Site 14: Barholm Road elongated dam........................................................................... 17 Site 15: Matthews Creek tributary - Middle Muroon Road .............................................. 17 Site 16: Dry conservation reserve – Muroon Road ......................................................... 18 Site 17: Old railway bridge (East Barwon) - SE ............................................................. 19 Site 19: Excavated billabong (East Barwon) .................................................................. 21 Site 20. Wetland behind Forrest football oval ................................................................. 22 Discussion ....................................................................................................................... 23 Primary refuge sites ....................................................................................................... 23 Gosling Creek Wetland............................................................................................... 24 Old railway bridge (Barwon Downs) – SE ................................................................... 24 Secondary refuge sites .................................................................................................. 25 Potential Conservation Wetlands ................................................................................... 26 Recommendations .......................................................................................................... 28 References ....................................................................................................................... 29 iii Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Introduction The Corangamite Regional Catchment Strategy 2013-2019 (CCMA 2013) has been developed to provide a vision for the integrated management of natural resources in the Corangamite region. One of the key components of the strategy is the objective for threatened flora and fauna: ‘To manage the threat of species extinction so that key populations are resilient and secure in the longer term.’ This paper aims to build on the knowledge and develop management recommendations regarding the threatened fish species dwarf galaxias (Galaxiella pusilla) and Yarra pygmy perch (Nannoperca obscura) in the Upper Barwon Catchment. These species are predominately wetland species and are dependent on refuge habitat during dry conditions. In 2009 the Department of Sustainability and Environment (DSE) and the Corangamite Catchment Management Authority (CCMA) conducted an extensive review of all sites within the Corangamite catchment where threatened fish species had been found. Results of this review were published in the report Significant Fish Species Protection under Dry Inflow Conditions (Saddlier et. al. 2009). Through this process and following an expert panel workshop, a number of management objectives were developed and a list of sites of higher priority was established. The CCMA subsequently developed the HERO Significant Fish Project (CCMA 2010). This Project has been divided into a monitoring plan, a long-term action plan for resilience works (involving re-vegetation works and fencing of waterways), and an emergency response action plan. The short-term emergency actions target non-migratory threatened fish species, dwarf galaxias (Galaxiella pusilla) and Yarra pygmy perch (Nannoperca obscura). Isolated populations of dwarf galaxias occurring in Gosling Creek and the upper East Barwon River have been monitored regularly (Ryan 2011), however, in recent years this population has been placed under constant pressure due primarily to unrestricted stock access, leading to a significant reduction in the long term security of this population. The aim of this study was to investigate other potential dwarf galaxias wetland habitat in the upper Barwon River catchment in an attempt to find additional populations. This process was intended to improve the level of understanding required for development of the new Corangamite Waterway Strategy. Background There are only two recognised populations of dwarf galaxias within the Corangamite region, one located in the Upper Barwon River catchment at Gosling Creek and the other further downstream of Barwon Downs in wetland margins of the East Barwon River. Dwarf galaxias is considered to be a non-migratory species adapted to wetland environments and is able to survive short periods with very little water (Saddlier et. al. 2009). In these habitats, it is typically found in marginal vegetation in still or slow moving waters of roadside ditches, swamps and backwaters of creeks. This species is small (as the name suggests) and ranges in length from 20 to 40 mm in length, is without scales, but has a distinct silvery-white belly. The males have three distinct longitudinal black stripes along each side of the trunk, and a distinct orange stripe between the mid and lowest black stripe. (Cadwallader & Backhouse 1983, McDowall 1980). It spawns in late winter– spring when females lay from 65–250 adhesive eggs usually on the underside of aquatic vegetation or on a hard surface such as a rock or timber which can be fertilised by a number of males (Saddlier, Hammer and Jackson 2010). Dwarf galaxias is endemic to south eastern Australia and occurs from the Mitchell River Basin in central Gippsland, westward where it is found in a variety of systems throughout southern Victoria, to the Cortina Lakes, near the Coorong in South Australia and in lowland regions of north Tasmania (Saddlier, Hammer and Jackson 2010). Dwarf galaxias is considered ‘Vulnerable’ in Australia (EPBC Act 1999), ‘Vulnerable’ in Victoria (DSE 2009) and is listed under the Victorian Flora and Fauna Guarantee Act 1988. 1 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment The maintenance of instream habitat is crucial for the survival of this species. In accordance with the draft action statement for dwarf galaxias, important management actions include stock exclusion fencing, maintenance of aquatic macrophytes, and maintenance of suitable water quality and water level (Saddlier, Hammer and Jackson 2010). A drought management strategy is recognised by Saddlier et. al. (2009) as a high priority and is supported by the CCMA through the HERO Project (CCMA 2010). Methods Site Selection GIS databases and imagery (MapInfo and LIDAR) were used to map the Upper Barwon River catchment in the vicinity of the Gosling Creek and Barwon Downs region to locate likely wetlands for assessment. Wetlands within the floodplain of East Barwon River channel and further upstream in tributaries of the system were selected as a priority for assessment. A full list of wetlands selected for survey are included in Table 1. Table 1: Priority Significant Species Survey sites 2 Environous report for Corangamite Catchment Management Authority 15 13 11 14 10 16 12 9 19 7 6 18 8 17 4 3 5 1 20 2 Legend Waterway – Blue Upper Barwon Landscape zone – Red Survey Sites – Green Stars Figure 1: Potential dwarf galaxias habitat inspected in this study Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment At the time of the surveys, significant rainfall had occurred resulting in flooding of the East Barwon River and its tributaries and floodplain habitat. Consequently, Yarra pygmy perch sites (generally more mainstream than Dwarf galaxias habitats) could not be investigated as planned. As a result, greater emphasis was given to assessing the potential refuge habitat of dwarf galaxias. Recent rainfall in this system was characterised by no rainfall from January through to April, with only small events of up to 6 mm occurring from this time to mid-May. Greater rainfall occurred in June with the largest event coinciding with the survey event where 31 mm was recorded on June 12 and 13 (Figure 1). The resulting flow event in the East Barwon River and tributaries provided the perfect opportunity to observe the interaction of offstream wetlands and the main discharge channels. Estimates of peak flow on June 13 were 223 ML/day at the East Barwon River Offtake and 1019 ML/day at the Callahan Creek Offtake (Victorian Water Resources Data Warehouse, 2013). Figure 2: Rainfall at Gerangamete for January to June 2013. Aquatic Fauna Surveys Based on previous sampling capture rates for these species the predominant survey method was actively sampling using fine mesh dip nets. Bait traps were also deployed overnight at site 17 to passively capture fish as the greater depth at this site precluded active sampling. In-situ water quality was undertaken using a TPS 90FL multimeter to measure temperature, electrical conductivity, pH, dissolved oxygen and turbidity. Fish identifications were confirmed from appropriate references including Kuiter et al. (1996) and McDowell and Frankenberg (1981). All species caught were measured (to nearest mm). Fish lengths were measured and calculated as Total Length (TL) for fish with rounded caudal, while Fork Lengths (FL) (measured to the bottom of the forked tail) were measured and calculated for fish with forked tails. 4 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Results The complete set of fish survey data for each site is provided in detail in the Appendix and a summary of results is provided in the following pages for each of the surveyed locations. The aim of the summary pages for each of the following 20 sites is to outline a range of factors for each site, including: the occurrence of previously identified threatened fish populations; the occurrence of newly determined dwarf galaxias populations; presence/absence of eastern gambusia (Gambusia holbrooki); whether stock exclusion fencing was present; the relative quality of riparian and instream habitat; security from disturbance; security of water supply and connectivity to main watercourse. 5 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Sites 1 and 2: Callahan Creek offtake Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion X X X Very High Water security Habitat quality Site security Connection to watercourse No High Medium Low Very Low X X X X Above the offtake, habitat was characterised by small interconnected pools containing low levels of aquatic vegetation. Security at the site was moderate due to protection in private property, however, the majority of the riparian vegetation consisted of exotic species. The large pool immediately upstream of the off-take appeared to lack significant habitat for dwarf galaxias of pygmy perch and appeared as though much of the habitat may dry completely during summer periods. Presumably some remnant pools persist, reflected in the presence of very low numbers of native fish captured (one mountain galaxias (Galaxias olidus) and two southern pygmy perch (Nannoperca australis)). Habitat below the offtake was dramatically different to that above the off-take, with fast flows being restricted to a relatively narrow watercourse. Stock exclusion fencing and native revegetation had improved terrestrial and aquatic habitat. Higher levels of aquatic vegetation were observed at this site compared to sites above the off-take, providing substantially greater levels of habitat for mountain galaxias which were observed in relatively high abundance compared to upstream. The higher instream velocity may however been prohibitive to the wetland specialised species such as dwarf galaxias and pygmy perch. 6 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 3: Wetland at Wendy James Yes Very High Water security Habitat quality Site security Connection to watercourse No X X X Presence of dwarf galaxias Presence of gambusia Stock exclusion High Medium Low Very Low X X X X This site appeared to provide reasonable habitat, with moderate levels of aquatic vegetation and water depth. A range of southern pygmy perch sizes were captured, suggesting that recruitment had been successful over recent successive years. It is unknown whether these individuals were permanent residents at this site or had migrated here when pools were connected during high flow events, as the permanency of water at this site is unknown. Although damage to this site by stock was evident (instream bank and bed damage and limited fringing wetland vegetation), positive aspects of this site include the persistence of aquatic vegetation, presence of southern pygmy perch and absence of eastern gambusia. These positive features together with the relative isolation of this site from the main water channel (apart from high flow events) contribute to it being considered a potential dwarf galaxias refuge site. Recommendations for this site include restriction of stock access and increasing the site depth (preferably by excavating adjacent to this pool rather than within it) to increase both the area and the ability of this site to maintain habitat through summer and/or drought conditions. 7 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 4: Causeway at Wendy James Yes Very High Water security Habitat quality Site security Connection to watercourse No X X X Presence of dwarf galaxias Presence of gambusia Stock exclusion High Medium Low Very Low X X X X This site has been modified substantially and appears to have been subjected to unrestricted stock access for some time. Riparian vegetation was predominantly made up of grasses, while instream habitat quality is considered poor, with aquatic vegetation present in only very low abundance. Despite considerable sampling, only one southern pygmy perch and one mountain galaxias were captured. It is likely that the majority of this site would be dry over dryer summer months, as deeper pool habitat was not observed. 8 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 5: Billabong on Mahers Road Yes Very High Water security Habitat quality Site security Connection to watercourse No X X X Presence of dwarf galaxias Presence of gambusia Stock exclusion High Medium Low Very Low X X X X On initial inspection, this site appeared to be excellent habitat for species such as dwarf galaxias, as aquatic vegetation was abundant throughout the site and a slightly deeper area was present at one end. A total of 27 southern pygmy perch were captured from this site, suggesting that this habitat was either permanently inundated or linked to waters at critical times, enabling this species to recolonise during wetter periods. This site represents a potential opportunity to work with the landholder to provide a refuge site into which dwarf galaxias can be stocked. If this site was to be considered for this purpose, it is recommended that (as in site 3), that a deeper permanent area adjacent to the site be created to provide a drought refuge for this species. Positive aspects of this site include the relatively deep water, persistence of aquatic vegetation, isolated location above the main channel, presence of southern pygmy perch and absence of eastern gambusia. Relatively easy restoration of this site would include excavation of a deeper pool along the western south western bank, stock exclusion fencing and revegetation. The close proximity to Mayers Road makes this a potentially good demonstration site. 9 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 6 and 7: Dewing Creek Weir and Rowlands Road Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion X X X Very High Water security Habitat quality Site security Connection to watercourse No High Medium Low Very Low X X X X Habitat near the offtake weir consisted predominantly of a very low amount of smaller woody debris as well as the boulder and rock which had been recently used to stabilise the bed and banks upstream and downstream of the newly constructed weir wall. Sampling at this site did not record the presence of any fish and it is possible that this site has been completely dry over the summer period. The complete lack of aquatic vegetation also suggests that this site has not received a permanent water supply over dryer months Sampling at Rowlands Road (300 m upstream) did not record the presence of fish or macroinvertebrates, suggesting that this site may be completely dry during the summer period. This was supported by the lack of established aquatic vegetation at this site. These two sites have a low conservation priority for dwarf galaxias due to low water security, poor aquatic habitat and potential for eastern gambusia to move into the stream. 10 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 8: Dewing Creek Weir Offtake Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion X X X Very High Water security Habitat quality Site security Connection to watercourse No High Medium Low Very Low X X X X While this site has been highly modified, resulting in a concrete lined channel, deep silt deposits have enabled dense levels of submerged aquatic vegetation to persist. This habitat supports moderate numbers of flat-headed gudgeon (Philypnodon grandiceps), which were easily captured from the channel bed. No other species were observed at this site. While this irrigation channel had potentially good water security, potential high water velocity (during irrigation periods) would be less suitable to the wetland specialised species. However, it is possible that regulation pondages associated with the irrigation systems (situated out of the main irrigation channel), could provide suitable refuge for these threatened species. 11 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 9: Gosling Creek Wetland Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion X Very High Water security Habitat quality Site security Connection to watercourse No X X High Medium Low Very Low X X X X A total of only two dwarf galaxias and three southern pygmy perch were captured from this site, despite extensive sampling efforts. While eastern gambusia was not captured in this assessment, they have previously been recorded at this location. Habitat at this site was dominated by high levels of submerged aquatic vegetation, predominantly water ribbon (Triglochin procera) and sedge species on a very soft silt/mud substrate. Cattle damage at this site was severe, with pugging being evident around the entire wetland margin as well as extending well into the wetland. This unrestricted cattle access has undoubtedly led to deterioration of instream habitat through the loss of aquatic vegetation, severe sedimentation and poor water quality and is most likely the reason for decreasing numbers of dwarf galaxias being captured at this site compared to previous sampling occasions. While some effort has been made to exclude stock and revegetate the surrounding area with native vegetation matching the Ecological Vegetation Class (EVC): Otway Plain EVC 53 – Swamp Scrub, resistance from the landholder to fence the wetland to prevent cattle access has undoubtedly had a major impact on population numbers. Under these circumstances, it is unlikely that this population will persist over the long term. It is clear that further negotiations are required with the landholder and where possible additional pressure should be applied to ensure compliance with obligations associated with this significant site. 12 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 10: Railway line dam off Gosling Creek Yes Very High Water security Habitat quality Site security Connection to watercourse No X X X Presence of dwarf galaxias Presence of gambusia Stock exclusion High Medium Low Very Low X X X X A total of three specimens of southern pygmy perch were captured from this location, however, eastern gambusia was not present. Habitat at this site was poor, as unrestricted cattle access has had an obvious impact on the riparian and instream habitat quality. Low levels of aquatic vegetation (sedge species) were present and provided some cover for the southern pygmy perch captured. Positive aspects of this site include isolated location above the main channel, presence of southern pygmy perch and absence of eastern gambusia. Restoration would include stock exclusion fencing, provision of offstream water points, excavation of a deeper pool and reintroduction of aquatic plant species. NO PHOTO TAKEN 13 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 11: Bird Reserve - Pennyroyal Valley Road Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion X X X Very High Water security Habitat quality Site security Connection to watercourse No High Medium Low Very Low X X X X This site represents a shallow wetland complex with two distinct (but linked) areas. The larger wetland had a maximum depth of approximately 30cm and possessed moderate levels of aquatic vegetation, while the second area was distinctly smaller in size but greater in depth (up to approximately 70cm depth) with moderate to high levels of aquatic vegetation. All fish captured from this site (southern pygmy perch) were found in the smaller (deeper) area. Although on private land, this site is used as a bird reserve, with a bird hide present on the wetland margin (between the wetland and road). Due to the protected nature of this site, this wetland habitat may represent an opportunity to work with the landholder to provide a conservation area for stocking with dwarf galaxias (or Yarra pygmy perch). If this option is considered, it may be advisable to increase the depth of some of the site to provide great water security and drought refuge zones within the wetland complex. 14 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 12: Pennyroyal Road Cottages Good quality habitat was observed at this site, with some dense patches of submerged aquatic vegetation present in both pools. Fish fauna consisted of two specimens of southern pygmy perch and over 300 eastern gambusia at this site. The dominance of eastern gambusia makes this site an unlikely candidate for dwarf galaxias translocation, as complete eradication of eastern gambusia and prevention of their return is very difficult to achieve. Sampling in the upper wetland did not record the presence of fish or macroinvertebrates, suggesting that this site may be completely dry during the summer period. NO PHOTO TAKEN 15 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 13: Pennyroyal Railway dam Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion No X X X Very High Water security Habitat quality Site security Connection to watercourse High Medium Low Very Low X X X X This site had presumably been created in a similar fashion to sites 16 and 17, in that it appears to have been created when earth was pushed up from the surrounding area to create an elevated terrace onto which the railway line could be constructed. Habitat at this site was poor with very little aquatic vegetation present. Because this site was difficult to sample using dip nets, a total of 10 bait traps were deployed overnight. Traps were set at dusk, during which time one specimen of short-finned eel (Anguilla australis) was observed in the shallows. Bait traps were retrieved early the next morning without result. The high elevation of this site provide some protection from invasion by eastern gambusia, and the relatively intact habitat, including stock exclusion fencing are positive aspects of this site as a conservation wetland. Its proximity to but separation from the Pennyroyal Creek may provide an ideal location for the preservation of Yarra pygmy perch that occur nearby below the offtake weir. The pine plantation along the margins of this billabong might however prove to be an impediment to the successful rehabilitation of this site. This plantation would currently shade aquatic vegetation and potentially add significant organic matter into the system. Replacement of the pine plantation with native vegetation would be a primary restoration activity. Other restoration activities would include removal of the predatory short finned eels, depth excavation (if necessary) and reintroduction of aquatic vegetation. NO PHOTO TAKEN NO WATER QUALITY TAKEN 16 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 14: Barholm Road elongated dam Yes No X X X Presence of dwarf galaxias Presence of gambusia Stock exclusion Very High Water security Habitat quality Site security Connection to watercourse High Medium Low Very Low X X X X Sampling at this site did not record the presence of fish or macroinvertebrates, suggesting that this site may be completely dry during the summer period. Wetland was a shallow depression without stock exclusion fencing and not considered to have a priority as a potential conservation wetland. NO PHOTO TAKEN NO WATER QUALITY TAKEN Site 15: Matthews Creek tributary - Middle Muroon Road Sampling at this site did not record the presence of fish or macroinvertebrates, suggesting that this site may be completely dry during the summer period. Instream habitat was of a reasonable standard with good fringing vegetation and some submerged vegetation, however the lack of overall water depth reflects the lack of water security present at this site. While eastern gambusia were not captured, it is highly likely that this species could recolonise this location. As a result, this location is not considered to have a priority as a potential conservation location. NO PHOTO TAKEN NO WATER QUALITY TAKEN 17 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 16: Dry conservation reserve – Muroon Road Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion X X X Very High Water security Habitat quality Site security Connection to watercourse No High Medium Low Very Low X X X X Although it was clear that this site was dry at the time of sampling, vegetation in the centre of this reserve and the spongy nature of the upper layer of soil and grass suggests that it may lie along a natural drainage line within the pasture. This was supported by the presence of frogs, which could be heard calling in the area. Positive attributes of this reserve include an excellent fringing tree cover, isolation from invasive pest fish and stock exclusion fencing. This location has potential as a conservation wetland, however, restoration efforts must include excavation to create deeper pools which are capable of retaining water throughout the year. It is therefore also necessary to determine the level of inflow into this site to establish whether sufficient surface runoff is available to maintain a constructed pool habitat. 18 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 17: Old railway bridge (East Barwon) - SE This site was presumably created after earthworks for the railway bridge (now under disrepair) created a deep depression and high earth wall to the north side. Habitat at this site was characterised by a deep pool, fringed by dense aquatic vegetation, dominated by ribbonweed (Triglochin procera) and Persicaria spp. This dense vegetation provides an ideal habitat for the large numbers of southern pygmy perch and dwarf galaxias found at this site (a total of 9 and 37 individuals respectively). A more recent investigation in July 2013 also found numerous dwarf galaxias but no eastern gambusia (pers. comm., Rhys Coleman, Melbourne Water, 2013). While the site is relatively secure from destruction as it can only to accessed via private property, the high potential for erosion or inundation is reason for concern. The elevation of this wetland appears to be at a critical height above the water level of the East Barwon to prevent the colonisation of eastern gambusia. Notably, eastern gambusia were not captured at the time of sampling, but during the rain event on June 12 and 13, elevated flow from the East Barwon River was flooding into the wetland. Other positive attributes of this site include diverse and abundant instream habitat, some riparian vegetation cover, sufficient water depth to provide water security in drought conditions and stock exclusion fencing. This wetland is considered to be the most important site for dwarf galaxias in the upper Barwon River catchment and should therefore be given the highest management priority. All efforts should be made to preserve the existing condition of the site from potential external influences such as eastern gambusia or sweet grass (Glyceria maxima). 19 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 18: Old railway bridge (East Barwon) - NW This habitat was presumably created in the same manner as that of site 17 and was just 40 m away on the northern side of the old railway bridge (~40m away) , with a high earth bank on the south side of the pool and a depression creating a long pool along the old railway line. Habitat at this site varied to that at site 16 in that the maximum depth was only around 80cm and had not been fenced off to cattle access (presumably due to the pool not posing a threat to cattle welfare). As a consequence, aquatic vegetation at this site was not as dense as that seen in site 16. The wetland was also at a lower elevation than site 17 and was flooded by the elevated river flows. As with Site 17, while this site can only be accessed from private land, the high potential for erosion or inundation is reason for concern. These factors were reflected in the range and number of fish species captured (three dwarf galaxias and greater than 250 eastern gambusia). It is significant to note that the three dwarf galaxias captured displayed some degree of caudal fin damage, presumably a result of aggressive behavior known to be displayed by eastern gambusia towards this species, particularly when present in high densities. As consequence the value of this site as a conservation wetland for dwarf galaxias is considered to be lower than for site 17. Shortened caudal fin (likely to have been nipped by eastern gambusia) 20 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 19: Excavated billabong (East Barwon) This site represents a permanent deepwater billabong, which has periodic connection with the main watercourse. Although stock have unrestricted access to this site, excellent habitat persists in the form of high levels of submerged and emergent aquatic vegetation that fringe the relatively deep waterbody. Consequently, high densities of fish were present, comprising high numbers of southern pygmy perch (66 were captured in total) and eastern gambusia (>200 individuals were observed). One specimen of dwarf galaxias was recorded at this site. As with sites 17 and 18, the high potential for erosion or inundation is reason for concern and results in a low site security score. Given the high numbers of eastern gambusia found at this site and the impacts this species has on dwarf galaxias, low numbers of this species are not surprising. It is unfortunate that the many positive aspects of this site (water permanency, habitat quality) are negated by the lower elevation of the wetland relative to the East Barwon River and presence of this introduced species. As a consequence, the value of this site as a conservation wetland for dwarf galaxias is similar to site 18 and is lower than for site 17. 21 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Site 20. Wetland behind Forrest football oval Yes Presence of dwarf galaxias Presence of gambusia Stock exclusion No X X X Very High Water security Habitat quality Site security Connection to watercourse High Medium Low Very Low X X X X On initial inspection, this wetland appeared to have been constructed well above the natural drainage line of the surrounding land, with no natural connection to watercourses within the area. Water level was low (less than 30cm) and habitat within the wetland was characterised by high levels of inundated grasses and some aquatic vegetation cover. Given the position of this wetland and the vegetation structure, it appears that this wetland periodically dries up over summer months and not surprisingly, did not appear to support a fish fauna. The ephemeral nature of this wetland makes it unsuitable as a refuge for fish species. Additionally, the high occurrence of the exotic sweet grass represents a significant risk to this wetland. NO WATER QUALITY TAKEN 22 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Discussion Most of the river frontage where dwarf galaxias have been captured in the upper Barwon catchment is privately owned and passes through a cleared rural landscape with a limited riparian zone and ‘less than 25% of original native vegetation remaining’ (CCMA 2013). The provision of additional wetland protection will rely on the participation of landholders. Stock exclusion fencing is likely to be the most important management tool for the restoration of native riparian vegetation. Livestock not only eat and trample the riparian vegetation, but also do the same to the aquatic vegetation and habitat. Protection and restoration of riparian (streamside) vegetation is also an important management tool. Riparian vegetation acts as a buffer zone for the instream environment and filters the hot summer sun, sediment input as well as other diffuse source pollutants such as nutrients and pollutants. It also contributes to organic matter within a waterbody (large woody debris, sticks and leaves) and provides a link between the terrestrial and the aquatic biota. Monitoring and management of the aquatic vegetation is likely to be an effective management tool for important dwarf galaxias populations. Management of aquatic vegetation may include water conductivity targets and appropriate wetting cycles for the taxa being managed which are predominately Triglochin, Myriophyllum, Vallisneria and Phragmites. Dwarf galaxias are reliant on an abundance and diversity of aquatic habitat for shelter, feeding and spawning. Conservation of this habitat is therefore of paramount importance when attempting to secure populations of this species. Natural flow regimes that regularly replenish the shallow freshwater habitats are essential for the survival of dwarf galaxias. Changes to natural flood and drying cycles, particularly in swamps and shallow creeks, through activities such as catchment clearing, establishing extensive plantations, construction of dams and direct extraction of water, pose threats to critical habitat (Saddlier et. al. 2010). Establishing greater water security should also be a priority for each of the primary refuge locations, particularly where additional water is available such as the upper and middle Barwon River catchment sites. In addition to these actions, it is important to recognise the potentially catastrophic effects of large numbers of eastern gambusia (as seen at site 18). Consideration must therefore be given to how secure a site is from colonisation by this highly invasive species. This process will also need to consider the water sources to supplement conservation wetlands and may involve finding alternative water sources and water filtration. Primary refuge sites As non-migratory species, the protection of primary refuge wetland habitat for dwarf galaxias is the most important management priority. This refuge habitat must provide shelter from high stream flows, sustained water quality and suitable instream habitat to provide shelter, feeding and spawning opportunities. Dispersion of these species from the refuge habitat is expected to occur predominately in a downstream direction. Throughout this investigation, where primary refuge habitat is identified, it is considered to have a higher management priority. Identification of these important primary offstream refuge and spawning sites should be a priority. Primary dwarf galaxias refuge wetlands identified include the wetland upstream of Division Road on Gosling Creek and the wetland on the south east side of the old railway bridge on the East Barwon River floodplain near Barwon Downs. Now that these sites have been identified, it is essential that a comprehensive maintenance plan be developed and include stock exclusion fencing, protection of water resources and flow regime and re-vegetation of the riparian zone. It is also important to undertake on-going water quality and non-destructive fish surveys to assess the sustainability of the populations. 23 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Gosling Creek Wetland The dwarf galaxias in Gosling Creek wetland (site 9) have been thought to be sustainable during previous surveys (Ryan 2011). Population numbers recorded during this current survey are the lowest recorded and some doubt exists on the ability of this species to maintain a robust population at this site. This is undoubtedly due (in part) to the impact of unrestricted stock access that occurs at this location. Cattle access has led to deterioration of instream habitat through the loss of aquatic vegetation, pugging of the wetland banks and poor water quality and is most likely the reason for decreasing numbers of dwarf galaxias being captured at this site compared to previous sampling occasions. Additional sediment is likely to have been deposited in the wetland due to a large erosional event upstream generated by a large flood event (1:100 ARI) in June 2012. In 2013 the CCMA completed extensive erosion control works upstream to prevent further sediment deposition downstream. While some effort has been made to revegetate the surrounding area with native trees and shrubs, resistance of the landholder to maintain stock exclusion fencing around the wetland has had a major impact on population numbers. It is likely that it is easier for the landholder to continue access to enable cattle to drink water and feed on the aquatic vegetation during dry periods. Under these circumstances, it is unlikely that this threatened population will persist over the long term. A more recent investigation in July 2013 found evidence of mature female dwarf galaxias, thus indicating breeding was still occurring (pers. comm., Rhys Coleman, Melbourne Water, 2013). This provides some additional confidence for the prospects of a population recovery if site restoration targets can be met. Recommendations for this site include continuing to encourage the landholder to fence off this site and instead encourage cattle access to the dam immediately upstream. There is an existing causeway between this dam and the dwarf galaxias wetland, thus providing a sufficient boundary for fencing and access. There appears to be sufficient water depth in the dwarf galaxias wetland, therefore further excavation is not required. A large floating mass of aquatic vegetation (predominately Myriophyllum) exists in deeper water outside the reach of the cattle. This mass of vegetation is likely to be providing refuge for the fish. Aquatic vegetation is likely to reestablish along the wetland margins once stock exclusion is achieved. Old railway bridge (Barwon Downs) – SE Of all sites supporting populations of dwarf galaxias, site 17 is the most significant. This is based upon a range of factors including the high numbers of individuals, the water holding capacity (affording the site some protection during drought periods), as well as the habitat quality and exclusion of stock from the site. It is possible that the presence of high numbers of southern pygmy perch at this site may also be a distinct advantage for the dwarf galaxias population. While the deleterious interactions of eastern gambusia towards dwarf galaxias are well known and have been directly observed during this survey (site 17), the impact of eastern gambusia on dwarf galaxias populations in the presence of high numbers of southern pygmy perch (and other native species) are not well understood. It is likely that complex interactions occur under these circumstances as is acknowledged by Rehage et al. (2004) who states that biotic interactions may enhance the resistance of communities to invasion and that invading organisms typically encounter a novel suite of competitors and predators, and thus their invasiveness may be affected by how they cope with these interactions. It must be noted that although we were unable to determine the exact depth of this pool, depth is obviously sufficient to support this healthy population of native species. Importantly, at the time of sampling, it was noted that the high rainfall event and subsequent overflow of the East Barwon onto the surrounding floodplain caused floodwaters to enter this site. Consequently, connection between this usually isolated pool and the greater Barwon River system was made 24 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment possible. As such, migration of species into this pool (including exotic species) and migration out of this pool (including dwarf galaxias) may occur. Significantly, eastern gambusia was not captured from this site, suggesting one of two scenarios. The first possibility is that this species has not entered the site in past flood events due to its higher elevation above the water level of the East Barwon River channel. Movement of small fish like eastern gambusia under flooding flow conditions would be difficult and therefore potentially restrict colonisation into this wetland. Conversely, it is also likely that the large population of southern pygmy perch population in this site is controlling eastern gambusia abundance. The critical aspect to this scenario is that there is sufficient diversity and quantity of aquatic habitat to provide refuge for the large dwarf galaxias and southern pygmy perch populations. Given that a connection between the main watercourse and this site currently exists and that the population of dwarf galaxias (and southern pygmy perch) appear to be in a very healthy state (with no sign of eastern gambusia persisting at the site), alterations to the flooding regime at this site should be discouraged. In short, without a full understanding of the complex interactions regarding flooding cycles and movement of eastern gambusia and dwarf galaxias to and from this site, abiotic changes to an apparently healthy population should be discouraged. The comparison of the two wetlands at this location provides the perfect opportunity to further investigate this relationship to provide information on the ideal wetland parameters required to conserve dwarf galaxias. Secondary refuge sites While the old railway bridge (East Barwon) – NW (site 18) is just 40m away from the primary refuge site (site 17), but has very different fish composition. While some dwarf galaxias were captured, the impact of eastern gambusia on individuals is clear. Low dwarf galaxias abundance was apparent and those captured showed obvious signs of aggression, presumably caused by the fin nipping of eastern gambusia. Control of eastern gambusia at this site is problematic, firstly due to the difficulty to completely eradicate this species from the site, and secondly, the inability to prevent further re-introductions during subsequent flood events. The lower elevation at this wetland would probably enable eastern gambusia to recolonise under most high flow events. One specimen of dwarf galaxias was captured from the ‘excavated billabong’ (site 19). Given the high numbers of eastern gambusia found at this site and the impacts this species has on dwarf galaxias, low numbers of this species are not surprising. As with site 18, it is unfortunate that the many positive aspects of this site (such as water permanency, good habitat quality) are negated by the presence of eastern gambusia. The occurrence of dwarf galaxias at these two floodplain wetlands demonstrates that this species may have dispersed in offstream wetlands throughout the Upper Barwon catchment. However, the difficulty in completely eradicating eastern gambusia from these low lying wetlands makes this site difficult to manage effectively and without substantial effort and resources. In an environment of increasingly scarce levels of both funds and physical resources, better value for money can be made by directing efforts to populations that do not contain large numbers of eastern gambusia. It should however, be recognised that as a result of substantial catchment modification, very few off stream wetlands have persisted in this catchment and any off-stream wetlands should be regarded as potential habitat for a range of threatened and non-threatened native fish species. These wetlands provide shelter for many fish species in times of high and low stream flow, and should be maintained as a matter of priority. As part of the on-going catchment restoration program, actions such as stock exclusion fencing along the elevated banks (above the appropriate flood level) and revegetation should be undertaken at these locations as a matter of priority. 25 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Potential Conservation Wetlands Another key conservation measure noted for dwarf galaxias is the translocation of fish into a conservation wetland. Key criteria for conservation wetlands for dwarf galaxias, in no particular order, include: lack of eastern gambusia; sufficient elevation to prevent re-colonisation of eastern gambusia; occurrence of other native wetland species; sufficient water quantity and water quality; sufficient pool volume and depth to sustain periods of drought as well as shallow ephemeral margins; abundant and diverse habitat particularly aquatic vegetation; stock exclusion fencing and riparian vegetation; and suitability as a demonstration / educational site. Potential conservation wetland sites identified in this assessment include the Wetland on Wendy James property (Site 3), the Billabong on Mahers Road (Site 5), the Bird Reserve on Pennyroyal Valley Road (Site 11), the wetlands at Pennyroyal Creek Cottages (Site 12) and the Dry Conservation Reserve on Muroon Road (Site 16). Ranking for each of these wetlands is presented in Table 2. Sites without the eastern gambusia populations indicate that recolonisation is less likely and will ultimately require less management resources. The complete eradication of eastern gambusia once they have been established is difficult and likely to be detrimental to other management practices. Therefore sites with eastern gambusia already present and/or likely to be reintroduced are not considered suitable as conservation wetlands. Based on this criterion alone, the wetlands at Pennyroyal Cottages are therefore not considered to be suitable as a priority conservation wetland. The elevation of the wetland above the 1:100 year flood level is also considered to be an important criteria to prevent infestation (Rhys Coleman, Pers. Comm. Melbourne Water, August 2013). In addition, the occurrence of other wetland native species is also considered to be a positive feature as it indicates suitability of the habitat and potentially represents a predatory influence on any re-introduced eastern gambsuia (particularly for southern pygmy perch and flat headed gudgeon). Sites with sufficient water security and pool depth are likely to involve fewer resources to establish as hydrological assessments and excavation would not be required. Conservation wetlands being developed by Melbourne water for dwarf galaxias are being excavated with deep pools to sustain periods of low flow and with gentle sloping banks to provide shallow ephemeral wetland habitat. Importantly, these wetlands are designed to dry out along the gently sloping margins to encourage greater productivity of various components of the wetland ecosystem that have evolved in ephemeral systems, including dwarf galaxias (Rhys Coleman, Pers. Comm. Melbourne Water, August 2013). An abundance and diversity of aquatic habitat is relatively easy to re-establish if not present and may include replanting aquatic plants and introducing large structures such as logs or rocks. Stock exclusion fencing is essential for all wetlands to protect the quality of the wetland habitat, and is also relatively inexpensive to achieve. A location with easy access and relatively high exposure (such as next to a regularly used road) would also have advantages in promoting the importance of dwarf galaxias and the importance of habitat restoration measures such as stock exclusion fencing. According to this criteria, the five potential conservation wetlands have been ranked in order of suitability and management practicality. The higher ranked wetlands are not impacted by eastern gambusia, and appear to have sufficient elevation and currently support predatory native species capable of controlling an invasion of eastern gambusia. They also appear to 26 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment have sufficient water depth and water security and therefore require less restoration, apart from stock exclusion fencing and riparian vegetation planting. These sites include 5 and 11 and are well-located adjacent roads thus providing good demonstration sites. Site 16 would require excavation and an investigation into the water availability and therefore is likely to be harder to establish. It does, however have existing fencing and good riparian vegetation and is located in an accessible area. Table 2: Ranking of Conservation Wetlands for dwarf galaxias translocation Criteria for Conservation Wetlands 27 Wetland at Wendy James (Site 3) Billabong on Mayers Road (Site 5) Bird Reserve Pennyroyal Valley Road (Site 11) Pennyroyal Creek Cottages (Site 12) Dry Conservation Reserve (Site 16) Eastern gambusia NO NO NO YES NO Elevation MODERATE MODERATE MODERATE MODERATE HIGH Other native species YES YES YES NO NO Water quantity and quality Wetland volume, depth and shallow margins LOW HIGH MODERATE MODERATE LOW MODERATE HIGH MODERATE MODERATE LOW Aquatic habitat MODERATE MODERATE MODERATE MODERATE LOW Fencing NONE NONE PRESENT PRESENT PRESENT Riparian vegetation GOOD NONE GOOD GOOD GOOD Demonstration/ education LOW HIGH HIGH MODERATE HIGH OVERALL RANKING 3 1 2 5 (NOT SUITABLE) Environous report for Corangamite Catchment Management Authority 4 Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Recommendations 1. Maintain and where possible without disturbance, enhance habitat at the Old Railway Bridge (East Barwon) – SE (site 17). (Actions to include permanent fencing, erosion control on the East Barwon, and Glyceria control) 2. Continue encouragement of landholder participation in stock exclusion fencing of the Gosling Creek wetland. 3. Undertake regular water quality, habitat condition and fish composition assessments at sites 17 and 9. 4. Investigate feasibility of developing conservation wetlands (sites 3, 5, 11, 16) 5. Undertake stock exclusion fencing and native planting at other floodplain wetlands (including sites 18, 19). 6. Wetlands assessed in this investigation (sites 3, 5, 9, 11, 16, 17, 18, 19) be adopted and implemented into the Corangamite Waterway Strategy as key conservation features of the upper Barwon Catchment. 7. Assess other floodplain wetlands in the Upper Barwon catchment based on Conservation Wetland Criteria in this report. 28 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment References Cadwallader, P. L. & Backhouse, G. (1983) A Guide to the Freshwater Fish of Victoria. (Government Printer: Melbourne). CCMA (2010) Pilot Emergency Response Action Plan for the Protection of Significant Fish Species. Corangamite Catchment Management Authority. CCMA (2013) Corangamite Regional Catchment Strategy 2013-2019, Corangamite Catchment Management Authority 2013 DSE (2009) Advisory List of the Threatened Vertebrate Fauna in Victoria - 2007, Department of Sustainability and Environment, Melbourne. McDowall, R.M. (1980) Freshwater Fishes in South-Eastern Australia. Reed: Sydney. Raadik, T. and Koster, K. (2000) Pre- and post-fishway construction assessments - Barwon and Moorabool Rivers, and Otway streams. Report for the Corangamite Catchment Management Authority. Department of Natural Resources and Environment, Arthur Rylah Institute for Environmental Research. Rehage, J.S., Barnett, B.K. and Sih, A. (2005). Behavioral responses to a novel predator and competitor of invasive mosquitofish and their non-invasive relatives (Gambusia sp.). Behavioral Ecology and Sociobiology 57: 256-266. Ryan T. and McGuckin J. (2008a). Selected fish surveys in the Barwon and Moorabool River catchments following the prolonged low flow period of 2006-2007. A report prepared by environous for the Corangamite Catchment Management Authority. Ryan, T. and McGuckin, J. (2007) Drought impact on river health in the Corangamite Region. Prepared for the Corangamite CMA. Ryan, T. (2011) Assessment of priority dwarf galaxias (Galaxiella pusilla) and Yarra pygmy perch (Nannoperca obscura) populations within the CCMA (2010). Report for the Corangamite Catchment Management Authority. Saddlier, S., Ryan, T and Wooley, J. (2009). Corangamite CMA — Significant fish species protection under dry inflow conditions. Arthur Rylah Institute for Environmental Research Technical Report Series No. 198. Department of Sustainability and Environment, Heidelberg, Victoria. Saddlier, S, and Hammer, M. 2010. National Recovery Plan for the Yarra Pygmy Perch Nannoperca obscura. Department of Sustainability and Environment, Melbourne. Saddlier, S., Jackson, J. and Hammer, M. 2010. National Recovery Plan for the Dwarf Galaxias Galaxiella pusilla. Department of Sustainability and Environment, Melbourne. Victorian Water Resources Data Warehouse (2013) “www.vicwaterdata.net” 29 Environous report for Corangamite Catchment Management Authority Refining dwarf galaxias Galaxiella pusilla habitat in the Upper Barwon River catchment Appendix: Summary of Fish Assessments No. Site Date 1 Callahan Creek - u/s offtake 12/06/2013 2 Callahan Creek - d/s offtake 12/06/2013 3 Wetland at Wendy James 12/06/2013 4 Causeway at Wendy James 5 Billabong on Mahers Road 6 7 Dewings Creek Weir Dewing Creek at Rowlands Road bridge 8 12/06/2013 12/06/2013 12/06/2013 12/06/2013 Irrigation channel at Dewing Creek Weir 12/06/2013 9 Gosling Creek Wetland 12/06/2013 10 Railway line dam off Gosling Creek 14/06/2013 11 Bird Reserve - Pennyroyal Valley Road 12/06/2013 12a Pennyroyal Creek Cottages - lower wetland 12/06/2013 12b Pennyroyal Creek Cottages - upper wetland Length Fish type No. (mm) G. olidus 1 57 N. australis 1 58 N. australis 1 56 G. olidus 4 G. olidus 1 63 G. olidus 1 51 G. olidus 1 60 Engaeus sp. 1 ~10 N. australis 1 54 N. australis 1 48 N. australis 1 34 N. australis 1 28 N. australis 1 47 G. olidus 1 50 N. australis 1 55 N. australis 1 46 N. australis 1 37 N. australis 1 36 N. australis 23 No fish us or ds No fish or inverts P. grandiceps P. grandiceps P. grandiceps P. grandiceps P. grandiceps G. G. N. N. N. N. N. N. N. N. N. N. N. N. N. N. G. G. G. G. 1 34 1 39 1 43 1 56 3 pusila 1 pusila 1 australis 1 australis 1 australis 2 australis 1 australis 2 australis 1 australis 1 australis 1 australis 1 australis 1 australis 1 australis 1 australis 1 australis 1 holbrooki 1 holbrooki 1 holbrooki 1 holbrooki 300 36 30 40 36 50 12/06/2013 13-14/6/13 A. australis 14/06/2013 15 Matthews Creek tributary - Middle Muroon Road 13/06/2013 16 Dry conservation reserve (Muroon Road) 13/06/2013 17 Old railway bridge (East Barwon) - SE 13/06/2013 18 Old railway bridge (East Barwon) - NW 13/06/2013 19 Excavation billabong (East Barwon) 14/06/2013 20 Wetland behind Forrest football oval 30 14/06/2013 G. G. G. G. G. G. N. N. N. N. N. pusila pusila pusila pusila pusila pusila australis australis australis australis australis Easter gambusia present No eastern gambusia 27 28 28 30 39 26 30 46 52 14 36 46 13 Pennyroyal Railway dam 14 Barholm Road elongated dam Comment 1 1 1 1 1 32 1 1 1 1 5 24 26 25 34 22 G. pusila 1 G. pusila 1 G. pusila 1 G. holbrooki 1 G. holbrooki 1 G. holbrooki 1 G. holbrooki 1 G. holbrooki 1 G. holbrooki 15 G. holbrooki 250 N. australis 1 N. australis 1 N. australis 1 N. australis 1 N. australis 1 N. australis 1 N. australis 60 G. holbrooki 1 G. holbrooki 1 G. holbrooki 1 G. holbrooki 1 G. holbrooki 1 G. holbrooki 200 G. pusila 1 P. australis 50 33 33 32 39 34 18 24 17 Observed No fish or inverts would dry out 10 bait traps - not fish of inverts Observed No fish or inverts would dry out No fish or inverts would dry out Wetland dry but fenced and well vegetated Only 5 - 10% habitat sampled 32 38 31 37 Serious fin nipping observed Observed 63 58 47 38 17 24 26 27 22 20 26 Observed 27 No fish or inverts would dry out (High above floodplain) Environous report for Corangamite Catchment Management Authority