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Marking Beans Part 2 – Advanced Population Study Lab
Name: _______________________
PART 2- Checking your assumptions
Background:
In tagging or mark recapture studies there are certain assumptions made about the population
of tagged and untagged individuals. These assumptions are beliefs about the behavior and
survival of these individuals. If the assumptions are violated (when our beliefs do not match
what actually happens) then the population estimate from mark recapture studies will be biased.
There are many advanced tagging models which are specifically parameterized to accommodate
when the assumptions of a basic tagging model are violated. These advanced tagging models use
additional parameters to ensure the estimates of abundances produced by these models are
unbiased.
Some Assumptions in Basic Tagging Studies (there are many more):
1. Tagged individuals are not killed by the tagging process (there is no short term instantaneous
mortality caused by tagging).
2. Tagged individuals completely mix with the untagged population immediately after tagging.
3. Tags are retained by the tagged individuals (there is no tag loss).
4. Over time the mortality (death rate) of tagged individuals is not different from the mortality of
untagged individuals.
5. The tag does not affect behavior.
6. Tagging is done at one point in time (each tagged individual has an equal probability of being
recaptured).
7. Tagged and untagged individuals have the same probability of recapture (includes
assumptions 1-6).
8. Species are not too rare (there is a relative high probability of capture).
9. The proportion of individuals tagged is a representative sample of the entire population (there
is a relative high probability of recapture).
Procedure:
1. Get your bag of white beans. Do not count the number of beans in your bag.
2. Your group will either choose or be assigned an assumption of mark recapture studies to
explore. Design a way to mimic a violation of this assumption, that is come up with an example
and manipulate the beans in the bag to show a case when the belief is not true. We will use black
beans again to represent marked individuals.
3. Collect data for the case where the assumption you have is violated.
4. Record data in the below table.
Assumption: _________________________
Total Marked (M)
(Number of Black
Beans Added to Bag)
Data Tables and Tables for Calculations
1
2
3
4
Sample
Size (K)
Marked in
Sample
(m)
Percent
Marked in
Sample (p)
Total
Population
Estimate
Mean
5
6
7
Standard Deviation
8
9
Standard Error of the
Mean
Sample
Size (K)
Marked in
Sample
(m)
Percent
Marked in
Sample (p)
Total
Population
Estimate
Population Estimate
𝑁=
𝑀 ×𝐾
1
=𝑀×𝑝 =
π‘š
(π‘‡π‘œπ‘‘π‘Žπ‘™ π‘€π‘Žπ‘Ÿπ‘˜π‘’π‘‘ )×(π‘€π‘Žπ‘Ÿπ‘˜π‘’π‘‘ 𝑖𝑛 π‘†π‘Žπ‘šπ‘π‘™π‘’ )
π‘†π‘Žπ‘šπ‘π‘™π‘’ 𝑆𝑖𝑧𝑒
Mean
𝑛
1
π‘₯Μ… = βˆ‘ π‘₯𝑖
𝑛
𝑖=1
Standard Deviation
10
βˆ‘π‘›π‘–=1(π‘₯𝑖 βˆ’ π‘₯Μ… )2
𝑆=√
π‘›βˆ’1
Standard Error (of the
Mean)
𝑆
π‘†π‘‘π‘Žπ‘›π‘‘π‘Žπ‘Ÿπ‘‘ π·π‘’π‘£π‘–π‘Žπ‘‘π‘–π‘œπ‘›
𝑆𝐸𝑋 =
=
π‘ π‘žπ‘Ÿπ‘‘ (π‘ π‘Žπ‘šπ‘π‘™π‘’ 𝑠𝑖𝑧𝑒)
𝑛
√
Questions:
1. What is the mean of your percent tagged fish from the 10 samples?
2. What is your mean estimate of the total population size from the 10 samples?
3. What is the standard error (of the mean) for the mean estimate of total population size
from the 10 samples?
4. How does your estimate compare to the estimate you had in part 1 (the earlier lab in
which the assumptions were not violated)? What was the result of violating the
assumption? Discuss why you think the estimate changed in this way.
5. Describe an additional reason that would lead to bias in the population estimate from a
tagging study (note: your reason should not be found in the list on the first page of this
worksheet)?