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Animal Use Protocol – Hazardous Agent Addendum (Chemicals / Toxins / Hazardous Drugs) IACUC # _________________ **All sections of this form must be filled out. Areas highlighted in [red] on this template are either recommended text to be reviewed for the particular agent (eg. Toxicity information) or recommended choices (eg. Recommendation of lab coat, gloves, and use of a fumehood/BSCII for a particular material). Once you have completed all areas of this form for your specific usage, taking into account the [red] recommendations, please change all text to [black]. Please remove these instructions prior to submission to the IACUC** 1. Identify the chemical / toxin / hazardous drug to be administered: Streptozotocin a. Briefly describe purpose: Click here to enter text. b. Specify the amount administered (e.g., dose, concentration, volume): Click here to enter text. c. Indicate the route of administration (include range of needle gauge if applicable): Click here to enter text. d. Indicate the frequency of administration: Click here to enter text. e. Indicate the maximum duration of exposure: Click here to enter text. f. Identify the location (Building / Room) where the chemical / toxin / hazardous drug will be administered: Click here to enter text. 2. Describe the predicted effects of the chemical / toxin / hazardous drug on the animal, i.e. toxicity to animal. Due to its selective toxicity towards beta cells of pancreatic islets, Streptozotocin is used to induce diabetes in experimental animals by causing inflammation and damage to the pancreatic islets. It specifically targets beta cells, entering via the glucose transporter GLUT2 and causing alkylation of DNA. DNA damage induces activation of poly ADP-ribosylation, depletion of cellular NAD+ and ATP, and formation of superoxide radicals, leading to the destruction of beta cells. Streptozotocin administration may result in hypoinsulinemia and consequent hyperglycemia. Streptozotocin is a known carcinogen and may also have mutagenic, cytotoxic, teratogenic and reproductive effects. 3. Chemical / toxin preparation prior to administration: a. Will any preparation of the chemical/toxin be required prior to administration (e.g. mixing, dilution, or dissolving of powders)? ☐ Yes ☐ No If yes, the following questions must be addressed: i. Describe the preparation process here: Click here to enter text. ii. Location where preparation will take place: Click here to enter text. iii. Will preparation be restricted to any of the following types of equipment? ☐ Chemical fume hood ☐ Class II Biosafety Cabinet connected to an exhaust system (hard-ducted or canopy-connected) ☐ Class II Biosafety Cabinet exhausted into the room ☐ Other, please specify: Click here to enter text. iv. Which pieces of personal protective equipment will be required for preparation? ☐ Gloves (material should be chosen to offer protection against the specific chemical to be used) ☐ Dust mask ☐ N95 respirator (enrollment in EHS fit testing program required) ☐ Other respiratory protection, please specify: Click here to enter text. ☐ Face Shield ☐ Protective laboratory eyewear ☐ Shoe Covers AUP Hazardous Agent Addendum (Chemicals / Toxins / Hazardous Drugs) OAWA Version Date: 07/2015 Animal Use Protocol – Hazardous Agent Addendum (Chemicals / Toxins / Hazardous Drugs) IACUC # _________________ ☐ Laboratory coat, gown or uniform ☐ Additional, please specify: double gloves 4. Describe how the chemical / toxin / hazardous drug is metabolized in the animal (include the excretion products, concentration and timeline). Metabolites of streptozotocin have been detected in urine up to 24 hours after administration, therefore bedding should be considered hazardous for 48 hrs post-administration. Up to 20% of administered streptozotocin is metabolized and/or excreted by the kidney. Data has shown that 60-70% of the administered dose of Streptozotocin was found in urine 4 hours postadministration, with 10-20% of the dose being unchanged drug. Excretion is negligible 36-48 hrs after administration. 5. Describe the safety procedures and personnel protective equipment research staff will use while administering these agents and while handling animals and animal bedding. a. Will administration occur using any of the following types of equipment? ☐ Chemical fume hood ☐ Class II Biosafety Cabinet connected to an exhaust system (hard-ducted or canopy-connected) ☐ Class II Biosafety Cabinet exhausted into the room ☐ Other, please specify: Click here to enter text. b. Which pieces of personal protective equipment will be required for administration and handling of animals and animal bedding? ☐ Gloves (material should be chosen to offer protection against the specific chemical to be used) ☐ Dust mask ☐ N95 respirator (enrollment in EHS fit testing program required) ☐ Other respiratory protection, please specify: Click here to enter text. ☐ Face Shield ☐ Protective laboratory eyewear ☐ Shoe Covers ☐ Laboratory coat, gown or uniform ☐ Additional, please specify: double gloves 6. Discuss the length of time the animals will be kept following exposure to the chemical / toxin / hazardous drug. Click here to enter text. 7. Animal housing: a. Following treatment, does the animal or its bedding pose a hazard to other animals, staff, or the environment? ☐ Yes ☐ No If yes, explain your answer. Bedding from animals that have been administered streptozotocin should be considered hazardous for 48 hrs post-administration. Veterinary staff will be notified 48 hours before administration and a door sign posted when using streptozotocin in the animal facility. The door sign will remain posted until bedding is no longer considered hazardous. Note that Streptozotocin is a U-listed hazardous waste agent and must be disposed of through EHS. Individuals who are pregnant or who may be pregnant should be advised of the risks associated with handling Streptozotocin and given the option to remove themselves from related procedures. b. Identify location(s) of animal housing post-exposure: Click here to enter text. 8. Will animals be returned to the animal facility following exposure? AUP Hazardous Agent Addendum (Chemicals / Toxins / Hazardous Drugs) ☐ Yes ☐ No OAWA Version Date: 07/2015 Animal Use Protocol – Hazardous Agent Addendum (Chemicals / Toxins / Hazardous Drugs) IACUC # _________________ If no, go to item 11. If yes, answer questions 9 -11 below. 9. Confirm that the animal facility manager or supervisor has been, or will be, consulted to ensure proper cage documentation and handling prior to initiating this work. ☐ Confirmed. 10. Please list precautions and safety procedures Veterinary Resources staff should follow when handling the animals and animal bedding. Specifically, identify which category of personal protective equipment (PPE) will be required for handling the animals and animal bedding: ☐ Standard PPE [facility scrubs or lab coat over street clothes, facility issued shoes, gloves (only when handling animals or cages) & particle mask (optional)] ☐ ABSL2 PPE (facility scrubs, facility issued shoes, lab coat, shoe covers, gloves & particle mask) ☐ Additional PPE, please specify: Click here to enter text. 11. If a toxin, is it classified as a select agent, but permissible below certain amounts (see list: http://www.selectagents.gov/PermissibleToxinAmounts.html)? ☐ Yes ☐ No a. If yes above, have you registered this toxin through the University’s due diligence program (https://afcf.umaryland.edu/ehs/sec/duediligence/)? ☐ Yes ☐ No AUP Hazardous Agent Addendum (Chemicals / Toxins / Hazardous Drugs) OAWA Version Date: 07/2015