Download Gram-Negative and Gram-Positive Bacteria, including Antibiotic

CleaningandDisinfectionProtocolforGram-Negativeand
Gram-PositiveBacteria,includingAntibioticResistantBacteria
Thisdocumenthasbeendevelopedinaccordancewithcurrentapplicableinfectioncontroland
biosecurityguidelines.Itisintendedforuseasaguidelineonly.Atnotimeshouldthisdocumentreplace
existingdocumentsestablishedbythefacilityunlesswrittenpermissionhasbeenobtainedfromthe
responsiblefacilitymanager.
PREFACE
Bacteriaarealargedomainofsingle-celled,prokaryotemicroorganisms.Typicallyafewmicrometresin
length,bacteriahaveawiderangeofshapes,rangingfromspherestorodsandspirals.Thevast
majorityofthebacteriainthebodyarerenderedharmlessbytheprotectiveeffectsoftheimmune
system,andafewareevenbeneficial.However,afewspeciesofbacteriaarepathogenicandcause
infectiousdiseases.Themodeoftransmissionforbacteriaischaracterizedbythespecificbacteria;
however,themostcommonroutesareviaindirectordirectcontactofinfectiousparticles,contactwith
orinhalationofrespiratorydroplets.Somebacteriamayalsobetransmittedbyingestionof
contaminatedfood,ormaternal-to-newborntransmission.VegetativeBacteriaincludingantibiotic
resistantorganismsareeasilyinactivatedbyroutinesurfacecleaninganddisinfection.Atpresentthere
isnoscientificevidencetoshowthatantibioticresistanceequatestochemicalresistance.
ThefollowinglistprovidesexamplesofsomepathogenicGramNegativeandGramPositiveBacteriafor
birds,mammalsandprimates.
Acinetobacteriwoffi
Leptospiragrippotyphosa
Rhodococcusequi
Bordetellaavium
Leptospirainterrogans
Salmonellaenterica
Bordetellabronchiseptica
Listeriamonocytogenes
Salmonellaenteritidis
Brucellaabortis
Micrococcusluteus
Salmonellatyphimurium
Burkholderiamallei
Moraxellabovis
Serratiamarcescens
Campylobacterjejuni
Morganellamorganii
Shigelladysenteriae
Chlamydiapsittaci
Mycobacteriumavium
Staphylococcusaureus
Corynebacteriumfaecium
Mycobacteriumbovis
Staphylococcusaureus(MRSA)
Corynebacteriumkutscheri
Mycoplasmacynos
Staphylococcusepidermidis
Corynebacterium
pseudotuberculosis
Mycoplasmamycoides
Staphylococcusepidermidis,
MRSE
Enterococcusfaecalis
Mycoplasmapneumonia
Staphylococcushyicus
Enterococcusfaecalis
(VancomycinResistant)
Pasteurellaanatipestifer
Staphylococcuspseudintermedius
Enterococcusfaecium
Pasteurellamultocida
Streptococcusagalactiae
Escherichiacoli
Proteusmirabilis
Streptococcusequi
zooepidemicus
Escherichiacoli(Extended
SpectrumBeta-lactamase)
Proteusvulgaris
Streptococcuspneumoniae
Fusobacteriumnecrophorum
Pseudomonasaeruginosa
Streptococcuspneumoniae
(PenicillinResistant)
Helicobacterpylori
Pseudomonasaeruginosa
(TetracyclineResistant)
Trueperellapyogenes
Pseudomonasmaltophilia
Yersiniaenterocolitica
Klebsiellapneumoniae
PREPARATION
Transmissionofvegetativebacteriacanbeattributedtodirectandindirectcontactandcontactwithor
inhalationofrespiratorydroplets.Somebacteriamayalsobetransmittedbyingestionofcontaminated
food,fecesormaternal-to-newborntransmission.Appropriatepersonalprotectionshouldbetakenfor
thoseresponsibleforthedecontaminationofacage,kennel,andstalloranimalhousingarea.
Appropriatebiosecuritypracticesshouldbeapplied,includinglimitingtheamountofaerosolsgenerated
anddisturbancetodust/soilintheareatobecleanedanddisinfected.
PROTECTIVEBARRIERS
Appropriatepersonalprotectionshouldbetakenforthoseresponsibleforthedecontaminationofthe
areaandmayincludethefollowing:
1. Disposablegloves.Glovesshouldbechangedasrequired(whentorn,whenhandsbecomewet
insidethegloveorwhenmovingbetweenanimalhousingareas)
2. Householdglovescanbeworn,buttheymustbediscardedwhenthecleaningiscomplete.
3. ProtectiveEyewear(goggles,faceshieldormaskwitheyeprotection)asappropriatebasedon
tasktobecompleted
4. Masks(surgicalorproceduralmaskssufficient)asneeded
5. Gownsasneeded
PRODUCTS
AllAcceleratedHydrogenPeroxide®(AHP®)disinfectant-cleanerproductshavebeenapprovedforsale
asadisinfectantbyeitherHealthCanadaortheUSEnvironmentalProtectionAgency(EPA)andcarry
eitheraDINorEPAregistrationnumberindicatingtheirapprovalforsaleasadisinfectant.
AHP®disinfectantsareregisteredasaHospitalGradeDisinfectantwhichdenotesthattheproductshave
beenprovenefficaciousagainstthethreemainsurrogatebacteriadesignatedbothHealthCanadaand
theEPAforBactericidalactivity;Staphylococcusaureus,PseudomonasaeruginosaandSalmonella
enterica(formerlyknownasSalmonellacholeraesuis)withcontactstimesof1minuteto10minutes
dependingontheformulationand/ordilutionused.Whilemanyregistereddisinfectantproductscarry
claimsagainstAntibioticResistantOrganismssuchasMRSAorVREitisimportanttounderstandthat
resistancetoAntibioticsdoesnotequatetochemicalresistance.
ProductName
Prevail™
Rescue™
Intervention™
Market
CompanionandFarmAnimal
CompanionAnimal
FarmAnimal
Country
Canada
USA
USA
RECOMMENDEDPROCEDURESFORCLEANINGANDDISINFECTIONOFANIMAL
CAREEQUIPMENT
Contaminatedanimalcareequipmentshouldbeclearlyidentifiedandkeptseparatefromclean
equipment.Animalcareequipmentinclude:bloodpressurecuffs,stethoscopes,thermometers,
handlingorrestrainingequipment,feedingandwateringappliances,etc.
1. UsetheAHP®solutiontoremovesoilandbodymaterials(e.g.blood,organicsoils)fromheavily
soileditems
2. ApplyAHP®solutionevenlyoversurfaceensuringthatallsurfacesarethoroughlywet.Items
thatcanbeimmersedmaybeplacedintoacontainerofAHP®solution.
3. AllowtheitemstoremainincontactwiththeAHP®solutionfortheappropriatecontacttime
(refertothelabeloftheAHP®solutionbeingused).
4. Afterthecontacttimehasbeenachievedremoveanyexcesssolutionwithacleancloth,sponge
orpapertowel.
5. Itemsthathavebeenimmersedshouldberemovedfromthesolutionaftertheappropriate
contacttime,rinsedwithpotablewaterandallowedtoairdry.
RECOMMENDEDPROCEDURESFORCLEANINGANDDISINFECTIONOFKENNELS
ANDCAGES:
TheAHP®technologyisdesignedtobebothanexcellentcleanerandeffectivedisinfectantandcanbe
usedinplaceofadegreaserordetergent.
1.
2.
3.
4.
Removeallanimalsandfeedfromtheareatobecleanedanddisinfected.
Removealllitterandfecalmatterfromfloors,wallsandsurfacesofkennelsorcages.
Emptyorcoverallfeedingandwateringappliances.
ThoroughlycleanallsurfaceswithsoaporadetergentoradilutedsolutionofAHP®andrinsewith
water.
5. ApplytheAHP®solutiontofloors,wallsandothersurfacesofthekennelsorcagesensuringthe
surfacesarethoroughlywetandremainwetfortheappropriatecontacttime.
6. Allowsurfacestodrybeforereintroducinganimalsbackintothekennelsorcages.
7. Cleananddisinfectallequipmentusedtoremovelitterandfecalmattersuchasforks,shovelsand
scrapers.
RECOMMENDEDPROCEDURESFORCLEANINGANDDISINFECTIONOFANIMAL
HOUSINGFACILITIES:
TheAHP®technologyisdesignedtobebothanexcellentcleanerandeffectivedisinfectantandcanbe
usedinplaceofadegreaserordetergent.
1. Removeallanimalsandfeedfromtheareatobecleanedanddisinfected.
2. Removealllitter,beddingandmanurefromfloors,wallsandsurfacesofcages,stallsorotheranimal
handlingareas.
3. Emptyorcoverallfeedingandwateringappliances.
4. ThoroughlycleanallsurfaceswithsoaporadetergentoradilutedsolutionofAHP®andrinsewith
water.
5. ApplytheAHP®solutiontofloors,wallsandothersurfacesofthecages,stallsorotheranimal
handlingareasensuringthesurfacesarethoroughlywetandallowtoremainwetforthe
appropriatecontacttime.
6. Allowsurfacestodrybeforereintroducinganimalsbackintothekennelsorcages.
7. Cleananddisinfectallequipmentusedtoremovelitter,beddingandmanuresuchasforks,shovels
andscrapers.