Download Biosolubilisation of brown coal by yeast

Biosolubilisation of brown coal by yeast
Bartosz Strzelecki, Natalia Kwiatos, Irena Romanowska, Hanna Miszkiewicz, Stanisław Bielecki
Keywords: biosolubilisation, brown coal, yeast
equipment required: flasks 500ml, tube 10 ml, centrifuge,
execution time: 14 days
Description: In this study is used following medium:
 YPG: glucose (20 g/l), yeast extract (10 g/l), peptone (20 g/l), (Agar for solid medium
20 g/l). The medium was set to pH 6,5 before autoclaving (20 min, 121oC).
Microorganisms used in experiments are deposited in collection IBT. Yeast are cultivated on
solid YPG medium for 7 days and then flask with 100 ml of fresh liquid YPG medium and 0.1
% (w/v) sterile powder of brown coal is inucolated sterilely. The culture is incubated for 7
days at 20oC and 180 rpm. Inoculum prepared in this way is ready to use. Biosolubilisation is
conducted added 5% (w/v) of sterile pretreated brown coal and leading process for 14 days
on the rotary shaker (200 rpm) at 30oC. Samples for analysis are collected in sterile condition
every few days.
Variants:

Biosolubilisation of brown coal by fungi

Biosolubilisation of brown coal by bacteria