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Biological Assessment Christian Wolter Leibniz-Institute of Freshwater Ecology and Inland Fisheries REFORM Summer School, Wageningen (NL), 28 June 2015 Aim Learn about the what and how to assess ecologic effects of hydromorphological change, degradation and rehabilitation Taxa Indicators & metrics Methods Application Aim What is lost or gained? How do biota respond? Kuban, Foto: Sukhodolov Elbe, Foto: Pusch What should be measured and how? Lahn, Foto: Hering Lahn, Foto: Hering What is lost • Littoral habitats • Habitat complexity • Large wood • Depth & width variability • Flow velocity patterns • Lateral connectivity • Flood plains Who is affected Floodplain vegetation Amphibians Mussels Snails Riparian vegetation Carabid beetles MI Juvenile fish Aquatic plants Adult fish Macroinvertebrates (MI) Adult fish Floodplain vegetation How to sample Transects through the active floodplain III II I I II III Foto: WSA Brandenburg How to sample Riparian vegetation 10-30 m buffer stripes along the river margins Foto: WSA Brandenburg How to sample Aquatic vegetation Hydrophytes & helophytes Helophyte abundance Weber et al. (2012) How to sample Aquatic vegetation How to sample Riparian beetles pitfall traps Pitfall trap Hand collection (source REFORM sampling protocols) How to sample Macro-Invertebrates How to sample Standard electric fishing Wading: 1 anode per 5 m wetted width Boat: 1 anode along the banks Single pass Fish How to sample Fish Potential electric fishing stretches How to sample Fish Add. gears in large rivers Methods overview Sampling objectives determine effort 1. Biodiversity / species inventories All gears/sources of evidence; qualitative sampling; all habitats, seasons, …; highest species identification efforts 2. Stock development / abundance trends Species of interests only; standardised gears / sampling; quantitative sampling; time series 3. Status assessment / measure evaluation Indicator taxa; standardised gears / sampling; quantitative sampling; all habitats; BACI Methods overview European methods 21 macrophyte-based assessment schemes Metrics: abundance, biomass, composition, diversity, growth forms 29 benthic inverts assessment schemes Metrics: abundance, biomass, composition, diversity 20 fish-based assessment schemes Metrics: abundance, biomass, composition, diversity, age structure Indicators Status assessment Biomass Abundance Benthic inverts Cyprinid fish Coregonid fish oligotrophic Submerged plants Emerged plants Percid fish Water quality hypertrophic Status assessment Indicators Growth forms (plants) Diversity Species composition Age structure (fish) low Habitat complexity/diversity high Indicators Status assessment Aquatic plants Benthic invertebrates Fish rheophilic limnophilic rheobiont larvae juv 0.3 adults 0.7 1.0 Flow velocity (m/s) 2.0 Status assessment Indicators More specifically … … gravel preferring and gravel-depending species e.g., lithophilic fish, gravel spawner with benthic larvae Foto: Jörg Freyhof Status assessment Indicators More specifically … at the reach level Fish assemblage integrates over functional process zones Functional process zone Fish zonation Fish Region Index Species Alosa fallax Barbus barbus Chondrostoma nasus Leuciscus leuciscus Salmo trutta ER MR HR EP MP HP 3 4 5 6 7 8 3 9 2 7 3 3 8 1 1 4 4 3 5 5 2 FRI S²FRI 7.75 6.08 5.83 5.75 3.75 0.20 0.45 0.33 0.93 0.57 Functional process zone Fish zonation Number of region-specific species How to assess what Riparian vegetation Floodplain vegetation Amphibians Mussels Snails Carabid beetles Juvenile fish Macroinvertebrates Aquatic plants Adult fish 1.Objectives 2.Indicators 3. Sampling strategy 4. Sampling sites