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Tokyo University Of Agriculture
How are plants and animals different?
•
If it is hot, we can move and find a cooler place
•
Plants cannot move so they have to adapt to survive extreme conditions.
Tokyo University Of Agriculture
Plants and animals are made from cells
onion
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Plant cells have some special features
Animal Cell
Plant Cell
+ vacuole (water storage)
+ cell wall (strength)
+ chloroplast (f00d)
Tokyo University Of Agriculture
Plant and animal cells are organised
DNA lives here
Tokyo University Of Agriculture
What is DNA?
•
DNA is a long thin
fiber
•
2 strands coil around
each other into a
double helix
•
Between the strands
are bases, A C T G
•
To fit inside a cell it
must be coiled up
into a chromosome
Tokyo University Of Agriculture
A chromosome
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DNA is organised into genes
Tokyo University Of Agriculture
The study of gene function =
genetics
Tokyo University Of Agriculture
What is my job at Nodai?
Tokyo University Of Agriculture
I study a plant called Physcomitrella
patens
I am a moss!
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Some facts about moss
•
Mosses are small
•
They are some of the oldest plants on earth
•
Very tough
•
Grow where bigger plants cannot
•
Easy to take care of in the lab
Tokyo University Of Agriculture
People working in the lab
Tokyo University Of Agriculture
Moss is easy to grow
Tokyo University Of Agriculture
Moss is easy to grow
Tokyo University Of Agriculture
I study proteins called AGPs
and their role in the cell wall
Tokyo University Of Agriculture
AGPs are involved in plant growth
Root
growth
Pollen
tube extension
Apoptosis
Somatic
embryogenesis
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Microscopy
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Microscopy
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Microscopy
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We use antibodies to detect AGPs in
plants
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Antibodies let us visualise the structure
of the plant
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To understand the role of the protein,
we must investigate the gene
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Genetic engineering
Insert into vector
Modify the gene
“Make more protein” = Over-express
“Make no protein” = Knockout
“Make it visible” = Fluorescent tag
Code for 1 protein
Put back into plant
Cut!
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Analyse transgenic plants
Overexpression
Knockout
More of the gene = bigger plants
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Why is genetic engineering useful?
•
Make stronger plants (no need for chemical fertilizers)
•
Make plants disease resistant
•
Increase crop yield (cheaper food)
•
Add more nutrition to the plant: e.g. golden rice
Increased β carotene
Prevents blindness
Tokyo University Of Agriculture
Summary
•
Plants play an important role in our daily lives
•
You can become a scientist if you study hard
•
My job has enabled me to travel to France,
Germany, Italy, USA, Czech Republic and Japan!
•
Maybe you can get a job in Manchester!
Tokyo University Of Agriculture
Thank you for listening!
My research is funded by
JSPS
Japan Society for the Promotion of Science