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Answers for student worksheet – Reverse Transcriptase
Slide 1
1) Reserve Transcriptase is a RNA-dependent DNA polymerase.
2) A polymerase is an enzyme, which is a protein in structure.
3) The reverse transcriptase shown in the picture is a crystallographic
structure, which composes of two subunits.
Slide 2
1) The function of the reverse transcriptase is to transcribe single strand RNA
into double-stranded DNA.
2) cDNA is the abbreviation for complimentary DNA.
3) Normal transcription involves the synthesis of RNA from DNA, while
reverse transcription involves the synthesis of cDNA from RNA.
Slide 3
1) The retro virus uses the reverse transcriptase during the process of reverse
transcription.
2) The retro viruses are RNA viruses, and it is found in HIV patients.
3) Without reverse transcriptase, the virus cannot replicate and continue to
reproduce.
Slide 4
1) Single strand of DNA is created from the viral Reverse Transcriptase.
2) Mutations often occur during the reverse transcription process and thus
casing drug resistance in the treatment of the cancer patient.
Slide 5
Antiviral drug is also commonly known as reverse transcriptase inhibitor.
Slide 6
1) RT-PCR is the abbreviation for reverse transcription polymerase chain
reaction.
2) Regular PCR applies to DNA strand only.
3) Reserve transcriptase is used in the medical field to produce insulin.