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RESULTS: Kirby Bauer Antibiotic Sensitivity Test/ Snyder Test The S. aureus (Gram +) plate: The S. aureus bacteria have zones of inhibition for all antibiotics tested (in fact, they overlap and can’t be measured). The only place that growth is seen is in the middle of the plate. The P. aeruginosa (Gram-) plate: Many of the antiobiotics did not develop zones of inhibition and are resistant. The E. coli (Gram -) plate: In the Kirby-Bauer table, E. coli is found under “Enterobaceriaceae”. Some antibiotics only work on Gram + or Gram - bacteria. We measure the diameter of the zone of inhibition in mm and look up in Kirby-Bauer Antibiotic Sensitivity table. If an organism is not listed in the table, we report “Cannot be determined”. In this picture, we can see colonies growing in the zone of inhibition. Each of these colonies would have to be isolated, grown in broth, and retested because it is highly possible that these bacteria are resistant to the antibiotic. It would be better to choose an antibiotic with a clear zone of inhibition. MIC- Minimum Inhibitory Concentrations are determined with agar-filled 96 well plates that have a variety of antibiotics at different concentrations. The \entire plate is inoculated with the test organism and is read by a machine. MIC is the lowest concentration of antibiotic that will kill the test organism.