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Plant-Based Biopharmaceutical Production under Controlled Environments Jessica Gamboa- University of Arizona Biosystems Engineering Chieri Kubota, Ryo Matsuda, Lucrecia M. Alvarez, & Guy A Cardineau Our Eventual Goal Use plant made pharmaceuticals to produce an easily deliverable form of the vaccine for the plague (Y. pestis) This will be done using plant-made pharmaceuticals grown under a controlled environment Plague Plague is an infectious disease that is caused by the bacterium Yersinia pestis, it can be deadly. The F1-V protein is used as an antigen fusion protein against plague. Experimental Goal To quantify… plant growth development photosynthesis total soluble protein concentration The tomato plants were modified to produce edible F1-V protein, a form of the vaccine for the plague Greenhouse growth is advantageous because high production and containment is achievable Items measured in experiment Stem growth Leaf production Light-saturated rate of photosynthesis in leaves Weekly fruit yield Total soluble-protein concentration F1-V concentration in fruits Plants Grown in Greenhouse ‘Durinta’ - Commercial greenhouse cultivator ‘TA234’ - Wild Type ‘T22.1’ - Transgenic line ‘T3D1.2’ - Supertransformed line Plant Growth and Leaf Production Stem Length ‘Durinta’>‘T22.1’=‘TA234’= ‘T3D1.2’ Number of Leaves ‘Durinta’= ‘T22.1’ =‘TA234’=‘T3D1.2’ Weekly Stem Elongation Averages 35 Centimeters 30 25 20 15 10 5 0 Durinta T22.11 T3D1.2 TA234 Weekly Leaf Development Number of Leaves 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 Durinta T22.11 T3D1.2 TA234 Photosynthesis, Fruit Yield and Total Soluble Protein Light-saturated rate of photosynthesis ‘Durinta’ ≥ ‘T22.1’ = ‘TA234’ = ‘T3D1.2’ Fruit Yield ‘Durinta’ > ‘TA234’ > ‘T22.1’ =‘T3D1.2’ Total Soluble Protein in Green Fruits ‘T22.1’ > ‘T3D1.2’ > ‘TA234’ >‘Durinta’ Conclusion Transgenic tomato plants showed a lower growth rate and lower yield, but produced more protein than ‘Durinta’, the commercial greenhouse standard. Transgenic tomato plants are considered promising candidates for high value protein production.