Download Department of Epidemiology - UCLA Fielding School of Public Health

Department of Epidemiology
School of Public Health
University of California, Los Angeles
Spring, 2008
Molecular Epidemiology of Cancer
EPI 243 (4 Units)
Tuesday and Thursday 10:00 AM-11:50 A.M., 71-257 CHS
Instructor:
Zuo-Feng Zhang, M.D., Ph.D.
Professor
Office: 71-225 CHS
Telephone:
825-8418
Fax:
206-6039
E-mail:
[email protected]
Office Hour: Thursday 12-2, or by appointment.
4/1 (wk 1.1)
Introduction - Course outline and requirements
Overview of the basic concepts of molecular epidemiology
4/3 (wk 1.2)
Molecular Markers of exposures
-Issues of the using adducts in epidemiologic studies
-Methods of Detection
-Protein adducts
-DNA adducts
-Serum/plasma concentration
Summary of Molecular Epidemiology
4/8 (wk 2.1)
Molecular Markers of Early Biological Response
Oncogenes and Tumor Suppressor Genes
-Discovery of oncogenes and tumor suppressor gene
-Mechanisms of oncogene and tumor suppressor gene formation
-Methods of Detection
-Function of oncogenes and tumor suppressor genes
-Oncogene/tumor suppressor genes and Epidemiologic Studies
4/10 (wk 2.2) Molecular Markers of susceptibility
-Potentially useful susceptibility markers
-Methods of Detection
-Phase I enzymes and genes (p450th)
-Phase II enzymes and genes (GST=s)
-NAT 1 and 2
4/15 (wk 3.1) Molecular Biology in Epidemiology
Dr. Jianyu Rao, Associate Professor of Pathology and Epidemiology
-Introduction
-Basic Principles of Molecular Biology
-Core Techniques of Molecular Biology
-Application
4/17 (wk 3.2) Design Considerations in Molecular Epidemiology (1)
-Introduction
-Using biomarkers in Epidemiological Research
-Molecular Epidemiologic Study Designs
4/22 (wk 4.1) Design Considerations in Molecular Epidemiology (2)
-Power estimate
-Interaction assessment
-Case-Case approach for interaction
4/24 (wk 4.2) Design Consideration: Biologic Specimen Collection, Process, Transportation and
Banking
-Utility of a bank for molecular epidemiologic studies
-Major influence on the utility of biologic banks
-Bias in studies involved banked materials
4/29 (wk 5.1) Design Consideration: Selection and Evaluation of Molecular Markers
-Properties of Markers
-Sate of Testing
Validation of Molecular Markers
-Measurement validity
-Internal Validity
-External Validity
Some Practical Considerations for Planning Genetic Association Studies Based on
TagSNPs
Shehnaz K. Hussain, Ph.D., Sc.M.
UCLA Division of Cancer Prevention and
Control Research, School of Public Health and
Jonsson Comprehensive Cancer Center
5/1 (wk 5.2)
Applications: Intermediate Markers in Chemoprevention
Jian-Yu Rao, MD
5/6 (wk 6.1)
Applications: Susceptibility Markers in Cancer Epidemiology
Zuo-Feng Zhang, MD, PhD
5/8 (wk 6.2)
Applications: Exposure Markers in Cancer Epidemiology
Binh Yang
5/13 (wk 7.1) New Technology: High-throughput SNP Identification
Jeanette Papp, Ph.D.
Assistant Adjunct Professor
Director, Sequencing and Genotyping Core
Department of Human Genetics
5/15 (wk 7.2) Summary of Molecular Epidemiology of Cancer
Zuo-Feng Zhang, MD, PhD
5/20 (wk 8.1) Final Examination
5/22 (wk 8.2) Molecular Epidemiology Lab (1)
(1) Collection of Buccal Cells (10-15 minutes)
(2) Centrifuge Buccal Samples
(3) Use of Pipette
(4) Sample digestion
5/27 (wk 9.1) Molecular Epidemiology Lab (2)
(1) extract DNA – Part I
(2) leave in freezer
5/29 (wk 9.2) Molecular Epidemiology Lab (3)
(1) extract DNA – Part II
6/3 (wk 10.1) Molecular Epidemiology Lab (4)
(1) set up for PCR – GST M1, T1, beta-globin
(2) PCR Amplification
6/5 (wk 10.2) Molecular Epidemiology Lab (5)
(1) load gel eletrophoresis
(2) run gel, get picture of gel
Course Grade
Participation
Lab
Final:
Letter grade:
10%
35%
55%
S/U
10%
35%
55%
For the letter grade, A, B, or C will be assigned according to percentiles and the distribution
curves of the scores by students.
Each week, 1-3 readings for next lecture will be handed out. Students will be expected to read
these papers.
Course Description
To introduce students to basic concept in molecular epidemiology of cancer, including molecular
biology in epidemiology, selection, evaluation, and validation of biological markers, Design
considerations statistical methods in molecular epidemiology. We will discuss practical issues
such as biologic specimen collection, transportation, process and banking. The measurements
and methodological issues of three major biological markers including exposure marker,
susceptibility markers, and earlier biological response markers will be discussed.
To introduce basic concepts and methodology of molecular epidemiology of cancer, and to
review current molecular epidemiologic research of cancer in recent medical and epidemiological
literature. S/U or letter grade.
Suggested Text Books for Epidemiology 243:
1. Schulte PA and Perera FP. Molecular Epidemiology. Academic Press, Inc., New York, 1993,
3-44.
2. Hulka BS, Wilcosky TC, Griffith JD (Eds). Biological Markers in Epidemiology. Oxford
University Press, New York, 1990
3. Franks LM & Teich NM (Editors): Introduction to the cellular & molecular biology of cancer.
Oxford University Press, Oxford, 1998.
4.Toniolo P, Boffetta P, Shuker D, Rothman N, Hulka B and Pearce N (eds): Application of
biomarkers to cancer epidemiology. International Agency for Research on Cancer (IARC)
Scientific Publication No. 142, 1997.