Download MAJOR SCIENCE COOL

Programmable Cool-Hotter
Dry Bath Incubator
INSTRUCTION MANUAL
_____________________________________________________________________
1
INDEX
Introduction----------------------------------------------------------------------------------------3
Controls and Features----------------------------------------------------------------------------4
Operation Instructions---------------------------------------------------------------------------6
Temperature Calibration-----------------------------------------------------------------------11
Troubleshooting Guide-------------------------------------------------------------------------13
Additional Information-------------------------------------------------------------------------14
Part List-------------------------------------------------------------------------------------------15
Warranty------------------------------------------------------------------------------------------16
Warning :
1.
2.
Read and carefully following manual instructions.
Do not alter equipment. Failure to adhere to these directions could result in
personal and/ or laboratory hazards, as well as invalidate equipment warranty.
2
1. Introduction
Overview
Programmable COOL-Hoter dry baths use interchangeable block modules for a
variety of applications including ligation, storage, restriction digests, denaturing DNA,
BUN, melting agar, coagulation studies, in situ hybridization and Hot Start
thermocycler reaction. All models incorporate a PI controller for easy temperature
selection, rapid heat up and cooling down with excellent stability. Temperature may
be set in 0.1 ℃ increments from -10℃ to 100 ℃. The unit contains “Constant
Operation” and “Programmable Mode. On “Constant Operation” can set Temperature
(from –10 ~ 100℃) and Timer (from 0 ~ 9999 mins). On “Programmable Mode” can
set 4 steps and cycle time(from 0 ~ 9 cycle). Each step can set Temperature (from –10
~ 100℃) and Timer (from 0 ~ 9999 mins). The unit can use RS232 port to do data
log.
Warnings
1. Use a properly grounded electrical outlet of correct voltage and current handing
capacity.
2. Disconnect from power supply before maintenance and servicing. Refer servicing
to qualified personnel.
3. In the event solution is accidentally spilled into the bath, disconnect grounded plug
and turn incubator upside down to avoid solution contacting the internal
components. Remove bottom cover and inspect to assure solution has not contacted
elements, thermostat or connector. Replace damaged parts.
4. Do not use in the presence of flammable or combustible material; fire or explosion
may result. This device contains components, which may ignite such materials.
5. Refer servicing to qualified personnel.
6. Ensure that the dry bath is connected to electrical service according to local and
national electrical codes. Failure to properly connect may create a fire or shock
hazard.
7. Always use the supplied handle to remove hot blocks to avoid burning your hand.
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2. Controls and Features
Please refer to figure 1 on the following page for the location of the different keys.
PAGE
SELECT PAGE
START
STOP
START and STOP Key
ENTER
Select Next or Last Page
Activate and stop operation
of the unit
ARROW Key (LEFT)
Moves cursor to the left
ARROW Key (UP)
Moves cursor upwards
Increase values
ARROW Key
(RIGHT)
Moves cursor to the right
ARROW Key
(DOWN)
Moves cursor downwards
Decreases values
SET Key
Enables the alteration of a
selected value
Heating
Indicate ON Heating
Status
Red LED light ON to
indicate Heating
Cooling
Indicate ON Cooling
Status
Green LED light ON
indicate Cooling
RS 232 Port
data
Power Switch
A Main Power Switch. Press
in “I” to switch on the power
supply. Press “O” to switch
off the power.
AC Socket
For AC Inlet and fuse Holder
I
O
Connect computer to record
4
INDICATE ON HEATING STATUS
ARROW KEY for RIGHT
ARROW KEY for UP
ARROW KEY for LEFT
Heating
Cooling
PAGE
ENTER
START
STOP
COOL-HOTER
DRY BATH INCUBATOR
INDICATE ON COOLING STATUS
SELECTPAGE
ARROW KEY for DOWN
START& STOP KEY
SETKEY
Control Pannel
AC INLET
RS 485 PORT
FUSE HOLDER
POWER SWITCH
I
O
Rare of the Unit
figure 1
5
3. Operation Instructions
Constant Operation :
1. Place the Dry Bath Incubator on a sturdy, level surface in a safe, dry place away
from laboratory traffic.
2. Ensure that the AC power switch is OFF, then plug the three-pronged power cord
into a grounded three-prong AC outlet of the appropriate voltage ( 115V or 220V
as indicated on the rating sticker near the AC cord on the back of the unit ).
3. Select a suitable module block and put it into the Dry Bath Incubator.
4. Switch the main power ON.
ENTER
5. Use
and
to select “Constant Operation”. And then press
.
Consta nt Op era tio n
TC
( 0.0)
6. Use
and
Min
0
to move cursor to which want to set Temperature or Time.
ENTER
7.
Press
and then use
and
ENTER
Finally, press
again.
START
STOP
8. Press the
to start heating or cooling.
START
STOP
9. Press
again to stop the unit.
6
to increase or decrease value.
Programmable Mode :
1. Place the Dry Bath Incubator on a sturdy, level surface in a safe, dry place away
from laboratory traffic.
2. Ensure that the AC power switch is OFF, then plug the three-pronged power cord
into a grounded three-prong AC outlet of the appropriate voltage ( 115V or 220V
as indicated on the rating sticker near the AC cord on the back of the unit ).
3. Select a suitable module block and put it into the Dry Bath Incubator.
4. Switch the main power ON.
ENTER
5. Use
and
to select “Programmable Mode”. And then press
.
Prog ra m m a b le Mod e
Prog ra m Num b er
( 00)
ENTER
6. Press
and then then use
and
to set program file number (It
ENTER
can maximum save 10 file from 00 ~ 09).
Press
again.
PAGE
7. Press
or
to next page.
Tem p.
Step 0 ( 0.0)
Step 1
0.0
Step 2
0.0
Step 3
0.0
7
Tim er
0
0
0
0
8.
Use
;
;
Step; Temperature or Time
and
to move cursor to which want to set
ENTER
9. Press
and then use
and
to increase or decrease value.
ENTER
Finally, press
10. Press
again.
to next page.
Cyc le Tim e s ( 00)
ENTER
11. Press
and then use
and
to increase or decrease value for
ENTER
cycle times. Finally, press
again.
START
STOP
12. Press the
to start heating or cooling.
START
STOP
13. Press
again to stop the unit.
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Annealing Program :
(This program application, please see page 15 for more detail.)
1. Place the Dry Bath Incubator on a sturdy, level surface in a safe, dry place away
from laboratory traffic.
2. Ensure that the AC power switch is OFF, then plug the three-pronged power cord
into a grounded three-prong AC outlet of the appropriate voltage ( 115V or 220V
as indicated on the rating sticker near the AC cord on the back of the unit ).
3. Select a suitable “Annealing Block” and put it into the Dry Bath Incubator.
4. Switch the main power ON.
ENTER
5. Use
and
to select “Annealing Program”. And then press
.
Anne a ling Prog ra m
Initia l Tem p.
95.0
Desc end ing Tem p. 5.0
Hold ing Tim e
5
Fina l Tem p.
4.0
6. Use
;
Parameter ---
;
and
to move cursor to which want to set
a. Initial Temp.：Start reaction Temp.
b. Descending Temp.：To decrease Temp. on each step
c. Holding Time：Holding time for each step
d. Final Temp.：Temp. for end step
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ENTER
7. Press
and then use
and
ENTER
Finally, press
again.
START
STOP
8. Press the
to start heating or cooling.
START
STOP
9. Press
again to stop the unit.
10
to increase or decrease value.
4. Temperature Calibration
Dry Bath Incubator with the optional block has been calibrated as a set. But, the
different kinds of block which the ÌT are not the same. For optimum accuracy
control temperature or change different kinds of block. Dry Bath Incubator should be
calibrated in accordance with the procedure outline below.
1. Insert a 45 mm calibrated laboratory Thermometer into the Thermometer holding
port which on the block.
ENTER
2. Please switch the main power OFF/ON and press
key simultaneously and
don’t release them until display over 1100, which on the up left of display. Please
see below. And then release them immediately.
1100
Consta nt Op era tion
Prog ra m m a b le Mo d e
3. Below page is under Calibration Mode.
INNER CALIBRATION
TARGETTEMPT. ( 75.0)
S:
0.0 R :
28.0
Tim e : 0
Ra te : 959
ENTER
4. Press
and then use
and
to increase or decrease TARGET
TEMP value. The target temperature is that you want to accuracy control
ENTER
temperature value. And then press
again.
START
STOP
5. Press the
to start heating or cooling.
6. Until “R” (Real Temperature) reach TARGET TEMP., the timer will be starting to
count up.
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INNER CALIBRATION
TARGETTEMPT. ( 75.0)
75.0
S:
0.0 R :
28.0
Tim e : 0
Ra te : 959
7. Until “Time” display 29 mins, you can set “S” value to the same as thermometer.
ENTER
And then press
.
INNER CALIBRATION
TARGETTEMPT. ( 75.0)
75.0
S : ( 0.0 ) R :
28.0
Tim e : 0
Ra te : 959
8. Please wait for some minutes, that microprocessor will auto adjust temperature
until LED display value is the same as thermometer.
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5. Troubleshooting Guide
Many operating problems may be solved by reading and carefully following the
instructions in this manual. Some suggestions for troubleshooting are given below.
Should these suggestions not resolve the problem, contact the SERVICE
DEPARTMENT or your distributor for assistance. If required, please include a full
description of the problem.
Problem
Comments
LCD do not light up
1. Check the fues (3A)
2. Ensure that the AC power switch is
ON
3. Check the three-pronged power cord
are properly plugged into a grounded
three-prong AC outlet of the
appropriate voltage.
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6. Additional Information
Maintenance
The Dry Bath Incubator may be cleaned with a moist cloth containing a mild soap
solution. The blocks are aluminum and may be cleaned with any of the commercial
aluminum cleaners on the market.
Specifications
a.
Model: MC-01N
b.
c.
d.
Microprocessor controller
Display: 128 x 64 Dot Matrix Display
Operating range: -10℃ to 100 ℃
e.
f.
g.
h.
Timer: 0 – 9999 mins
Program: Max. 4 steps.
Cycles Times: 0 ~ 9
Adjust the temperature: in 0.1 ℃
i.
j.
Outside Dimension: 20 x 29 x 14 cm (W x D x H)
Block Dimension: 125 x 90 x 40 mm (W x D x H)
k.
Operation Voltages: 110 or 230 voltage 50/60 Hz
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7. Parts List
Item
Parts Number
Description
1
MC-01-CPU
CPU Board
2
MC-01-PB
Power Board
3
MC-01-TECPB
TEC Power Board
4
MC-01-TC
TC Thermocouple K Type
5
MC-01-F
Fuse 4A/250V
6
MC-01-NF
Noise Filter; Model YL06TI
7
MC-01-PS
Power Switch
8
MC-01-MT
Main Transformer
9
MC-01-BR
Bridge Rectifiers, 20A/600V
10
MC-01-FAN
Fan, 110 V
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8. Warranty
MAJOR SCIENCE., warrants apparatus of its manufacture against defects in
materials and workmanship, under normal service, for one year from the date of
receipt by the purchaser. This warranty excludes damages resulting from shipping,
misuse, carelessness, or neglect. MAJOR SCIENCE liability under the warranty is
limited to the receipt of reasonable proof by the customer that the defect is embraced
within the terms of the warranty. All claims made under this warranty must be
presented to MAJOR SCIENCE within one year following the date of delivery of the
product to the customer.
Major Science Co., Ltd.
No. 7, Hwai-Der Street, Pan-Chiao City, Taipei Hsien 220, Taiwan
Tel : 886-2-2256-6980
www.major-sci.com.tw
Fax : 886-2-2256-6942
E-mail : [email protected]
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