Download Study of Cell Binding and Internalization

Study of Cell Binding and
Internalization of Immunoliposomes
on Human Cancer Ovarian
Cells(SKOV3)
By Sumaiya Tasneem
Introduction
• Many people say that they are going to find the cure for
cancer, however no cure for cancer has been discovered
yet.
• Nevertheless, numerous number of scientists and
researchers are working rigorously at it in order to find an
instant treatment.
• Ranging from chemotherapy and radiotherapy to
individual cancer drugs, it could be seen that there are
various treatments that are available.
• There is a new type of technique being worked on in order
to insert drugs targeting cancer cells into the body. The
drugs are going to be inserted into the body by means of
liposomes.
What are liposomes?
• A liposome is basically a
small spherical artificial
vesicle that holds drug inside
it.
• The walls are made of lipid
bilayers (amphiphiles) which
help the drug stay in and
prevent it from leaking out
while it has not reached its
specific site.
• In order for the liposome to
bind to a specific site,
antibodies are attached to
them with polymers.
Liposomes (cont’d)
• Liposomes with antibodies
(immunoliposomes) are
specified to attach to particular
cells, in our case it is cancer
cells.
• Cells have receptors called
antigens, which bond noncovalently to antibodies. That
means that the liposomes
attached to the antibodies are
going to attach themselves to the
cell, and possibly release their
drug inside the cell.
• Through the process of
endocytosis, the liposomes are
going to internalize into the
cells.
Overview of Experiment
• In this experiment, we are going to observe how many antibodies
we attach to the liposomes and how many of the liposomes bind to
target cells.
• Human cancer ovarian cells(SKOV3) are going to be used as our
target cells where the liposomes are going to release the drug.
• The antibody Herceptin also known as (Trastuzumab) is going to
be attached to polyethylene glycol (PEG), a polymer which is
attached to the liposome.
• Liposomes without antibodies are going to be used as our control,
whereas our experimental factor is going to be the liposomes with
antibodies.
• At the end of the experiment, we will observe the amount of
binding and internalization done by the different liposomes.
Materials
• Liposomes
• Polyethylene Glycol
(PEG)
• Human cancer ovarian
cells (SKOV3)
• Herceptin(Trastuzumab)
antibody
• Spectrofluorometer
Method
• Preparation of
liposomes with
malemide PEG.
• Activation of antibody
by Traut’s reagent.
• Binding of antibodies to
liposomes.
• Cell binding with
immunoliposomes and
control liposomes.
Connection to Human Life
• Antibodies help to specifically target the cancer
cells.
• This aim of these experiments is to design a
liposomal system that would specifically target the
cancer cells and reduce the toxicity.
• If the drug distribution in the cells work, then the
cancer cells specifically will be weakening,
causing the healthier cells to take over.
Acknowledgements
•
•
•
•
•
Professor Stavroula Sofou
Shrirang Karve
Polytechnic University
Dr. SAT Bhattacharya
Harlem Children Society
Work Cited
• http://www.unizh.ch/onkwww/lipos.htm
• http://www.cancerhelp.org.uk/help/default.asp?pa
ge=156
• http://www.avantilipids.com/PreparationOfLiposo
mes.html
• http://www.bartleby.com/65/li/liposome.html
• PEG-Immunoliposome, Bioscience Reports, Vol.
22, No. 2, April 2002, School of Pharmaceutical
Sciences, Teikyo University, Japan