Download Human Xenograft Models

Human Xenograft Models
Introduction
Mouse models of cancer have consistently been used to determine the in vivo activity of new anti-cancer
therapeutics prior to clinical development and testing in humans.
The most common models are xenografts of human tumors and cell lines grown subcutaneously in
immunodeficient mice such as athymic (nude) or severe combined immune deficient (SCID) mice. These
mouse strains exhibit very high take rates for xenografts, making them ideal hosts for in vivo propagation
of human tumor cells.
Xenograft tumors are usually established via subcutaneous inoculation of a predetermined number of
tumor cells into the flank of nude mice. Xenograft models are commonly used to determine ideal drug
dosing, treatment schedules, and routes of drug administration that maximize anti-tumor efficacy and
therapeutic window. The following are a few examples of cancer xenograft models performed by Noble
Life Sciences.
Antitumor Activity
of Gemcitabine in the ASPC-1
Xenograft
Protocol
Human Pancreatic Cancer Xenograft Model
Human cancer cells were injected
subcutaneously into the right flank of 8 week old
female NU NU nude mice (Crl:NU-Foxn1nu):
4000
)
ASPC-1 human pancreatic cancer cells

HCT-116 human colon cancer cells

A549 lung cancer cells

SKOV-3 ovarian cancer cells
Vehicle Control
Gemcitabine 100 mg/kg IP Days 12, 19, & 26
3
Tumor Volume (mm

Antitumor Activity of Gemcitabine in the ASPC-1
Human Pancreatic Cancer Xenograft Model
3000
2000
1000
0
Tumor size measurements were initiated 8 days
post inoculation and monitored three times per
week.
Tumor measurements were evaluated using
calipers; tumor size was calculated using the
2
following formula: MIN (L:W) x MAX(L:W)/2 .
Data are plotted as mean tumor volume +/standard error of the mean.
5
10
15
20
Treatment
Tumor Volume (mm3)
on Day 36
Vehicle
1,599
Gemcitabine
1,171
1200
400
200
0
3
Tumor Volume (mm
3
600
)
1400
1200
)
1400
800
1000
800
600
400
200
10
15
20
Days after Tum or Inoculation
25
40
45
T/C Value (%)
on Day 36
P value
0.019
1000
800
600
400
200
0
0
5
35
Subcutaneous SKOV-3
Human Ovarian Cancer
Xenograft Model
1200
1000
30
73
1400
0
25
Days after Tum or Inoculation
Subcutaneous A549
Human Lung Cancer
Xenograft Model
Tumor Volume (mm
Tumor Volume (mm
3
)
Subcutaneous HCT-116
Human Colon Cancer
Xenograft Model
0
0
10
20
30
Days after Tum or Inoculation
40
0
10
20
30
40
50
Days after Tumor Inoculation
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